Overexpression of sweetpotato expansin cDNA (IbEXP1) increases seed

yield in Arabidopsis

 

Jung Myung Bae1,*, Man Sup Kwak1, Seol Ah Noh1, Mi-Joung Oh1, Youn-Sung Kim2, Jeong Sheop

Shin1

 

1Division of Life Sciences, Korea University, Seoul 136-701, Korea

2NH Seed Research and Development Center, Gyeonggi-do 456-824, Korea

 

Col-0 IbEXP1-ox #1 IbEXP1-ox #4 IbEXP1-ox #22

Protein (μg)a 3.18±0.04 5.04±0.16 5.01±0.11 4.04±0.18

Starch (μg)b 0.98±0.06 1.55±0.13 1.48±0.03 1.54±0.06

Table S1 Protein and starch content in IbEXP1-ox seeds.

aTotal protein content per each seed. One hundred mature dry seeds from Col-0 or Ib-EXP1-ox plants were used.bTotal starch content per each seed. Mature dry seeds (1 g) from Col-0 or IbEXP1-ox plants were used. Starch content per seed was calculated on the basis of the 1,000-seed weight in Table 1.

0

10

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30

Col-0 Exp-1 Exp-4 Exp-22Ros

ett

e le

af l

en

gth

(m

m)

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4

8

12

Ros

ett

e le

af w

idth

(m

m)

Col-0 Exp-1 Exp-4 Exp-22

a

bc c

ab

c bc

Fig. S1 Rosette leaf development in IbEXP1-ox Arabidopsis plants (a) Rosette leaf length of IbEXP1-ox plants at 3 weeks after planting. (b) Rosette leaf width of Ib-EXP1-ox plants at 3 weeks after planting. (a and b) Rosette leaf measurements were made on the fourth rosette leaf to emerge from T3 homozygous plants of each line. Mean values of >12 plants with standard deviations are shown. Different letters above the bars indicate significantly different means (P < 0.05) as analyzed by Dun-can’s multiple range test using the IBM SPSS Statistics 21 program

a

b

Fig. S1

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60

0

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Ro

sett

e le

af le

ng

th (

mm

)R

ose

tte

leaf

wid

th (

mm

)

Col-0 Exp-1 Exp-4 Exp-22

Col-0 Exp-1 Exp-4 Exp-22

Fig. S2

a

b

Fig. S2 Rosette leaf size in IbEXP1-ox lines at reproductive stage. (a and b) Size of rosette leaves. The length (a) and width (b) of fourth rosette leaf to emerge from T3 homozygous plants of each line was measured. Data were collected from Col-0 and Ib-EXP1-ox lines at 6 weeks after planting. Mean values of >12 plants with standard devi-ations are shown. Different letters above the bars indicate significantly different means (P < 0.05) as analyzed by Duncan’s multiple range test using the IBM SPSS Statistics 21 program.

a a a a

a a a a

Exp-1 Exp-4 Col-0

Fig. S3 Rosette leaf development in IbEXP1-ox lines at reproductive stage. Picture was taken at 8 weeks after planting.

Fig. S3

b

c

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leng

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Col-0 Exp-1 Exp-4 Exp-22

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1.6

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que

thic

knes

s (m

m)

Col-0 Exp-1 Exp-4 Exp-22

2.0

Exp-1 Col-0a

Fig. S4

Fig. S4 Silique phenotype in IbEXP1-ox lines. (a - c) Silique size comparison between Col-0 and IbEXP1-ox plants. Thickness (b) and length (c) of siliques were measured with siliques on the primary inflorescence stem at 6 weeks after planting. Different let-ters above the bars indicate significantly different means (P < 0.05) as analyzed by Duncan’s multiple range test using the IBM SPSS Statistics 21 program. Mean values of >50 siliques from at least six different plants with standard deviations are shown.

a

a

b b b

a aa

Fig. S5

Col-0 IbExp1-ox

Fig. S5 Branching phenotype in IbEXP1-ox plants. Picture was taken prior to seed har-vest at 7 weeks after planting. Aerial part of individual Col-0 or IbEXP1-ox plant #1 in Fig. 4 was enlarged.