pemanfaatan ekstrak etanol buah sosis (kigelia...
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PEMANFAATAN EKSTRAK ETANOL BUAH SOSIS (Kigelia africana (Linn.)
Benth.) SEBAGAI ANTIOKSIDAN ALAMI DAN ANTIBAKTERI DALAM
PEMBUATAN SABUN TRANSPARAN
UTILIZATION OF SAUSAGE FRUIT (Kigelia africana (Linn.) Benth.)
ETHANOL EXTRACT AS A NATURAL ANTIOXIDANT AND
ANTIBACTERIAL IN TRANSPARENT SOAP
Oleh,
Birgitta Eknis Putri
NIM: 652010006
TUGAS AKHIR
Diajukan kepada Program Studi: Kimia, Fakultas: Sains dan Matematika guna
memenuhi sebagian dari persyaratan untuk mencapai gelar Sarjana Sains (Kimia)
Program Studi Kimia
Fakultas Sains dan Matematika
Universitas Kristen Satya Wacana
Salatiga
2014
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PEMANFAATAN EKSTRAK ETANOL BUAH SOSIS (Kigelia africana (Linn.)
Benth.) SEBAGAI ANTIOKSIDAN ALAMI DAN ANTIBAKTERI DALAM
PEMBUATAN SABUN TRANSPARAN
UTILIZATION OF SAUSAGE FRUIT (Kigelia africana (Linn.)
Benth.) ETHANOL EXTRACT AS A NATURAL ANTIOXIDANT AND
ANTIBACTERIAL IN TRANSPARENT SOAP
Birgitta Eknis Putri*, Hartati Soetjipto**, Sri Hartini**
*Mahasiswa Program Studi Kimia Fakultas Sains dan Matematika
**Dosen Program Studi Kimia Fakultas Sains dan Matematika
Universitas Kristen Satya Wacana, Salatiga
Jl. Diponegoro No. 52-60, Salatiga 50711, Jawa Tengah – Indonesia
ABSTRACT
The objectives of this study were to determined the total phenolic content, antioxidant
activity and antibacterial activity of ethanol extract of the sausage fruit as the natural
active ingredients in soap transparent. The sausage fruit extract was obtained by ethanol
maceration method. The total phenolic content was measured by folin ciocalteau
reagent, antioxidant activity was test by using DPPH (1,1-Diphenyl-2-Picryl-hidrazyl)
reagent where as antibacterial activity was performed by using paper discs method
againts gram positive bacteria [Bacillus subtilis (ATCC 6051)] and gram negative
bacteria [Escherichia coli (ATCC 0091IFO)]. The quality of transparent soap which is
obtained in this study was compared to SNI 06-3532-1994 bath soap. The extract was
concentration were used in this study are 0%; 0,5%; 1,0%; 1,5%; 2,0%; 3,0%; and
4,5%. the data were analized by Randomized Completely Block Design (RCBD), 7
treatments and 4 replications. The average total phenolic content, antioxidant
activity and IC50, and Minimum Inhibitor Concentration (MIC) were determined with
the test Honestly Significant Difference (HSD) with significance level 0f 5%. The
results of the study showed that the total phenolic content of the extracts was
6,45±0,59 mg EAG/ g and antioxidant activity with IC50 8,56±0,84 mg/ L. Antibacterial
activity of the sausage fruit extract was more effective to gram positive than gram
negative bacteria. The optimal concentration of fruit extracts in transparent soap making
process was 2,0%. The transparent soap obtained fulfilled SNI 06-3532-1994, except
for the value of moisture content.
Keywords: Kigelia africana, polyphenols, antioxidant, antibacterial, transparent soap