Product Information and Testing - Amended

©2010 WiCell Research Institute The material provided under this certificate has been subjected to the tests specified and the results and data described herein are accurate based on WiCell's reasonable knowledge and belief.  Appropriate Biosafety Level practices and universal precautions should always be used with this material.  For clarity, the foregoing is governed solely by WiCell’s Terms and Conditions of Service, which can be found at http://www.wicell.org/privacyandterms. 

Product Information

Testing Performed by WiCell

Test Description Test Provider Test Method Test Specification Result

Post-Thaw Viable Cell Recovery WiCell SOP-CH-305 ≥ 15 Undifferentiated Colonies, ≤ 30% Differentiation Pass

Identity by STR UW Molecular Diagnostics Laboratory

PowerPlex 1.2 System by Promega

Consistent with known profile Pass

Sterility - Direct transfer method Apptec 30744 Negative Pass Mycoplasma Bionique M250 No contamination detected Pass Karyotype by G-banding WiCell SOP-CH-003 Normal karyotype Pass

Amendment(s):

Reason for Amendment Date

CoA updated to include copyright information. See signature

CoA updated for format changes, including adding fields of thaw recommendation, vial label, protocol, and banked by, and removal of footnotes. 08-JUL-2010

CoA updated for clarification of passage number 01-NOV-2010

Original CoA 14-SEP-2010

Product Name WA07 Alias H7 Lot Number WB0024 Parent Material WA07-MCB-05 Depositor University of Wisconsin – Laboratory of Dr. James Thomson Banked by WiCell Thaw Recommendation Thaw 1 vial into 3 wells of a 6 well plate. Culture Platform Feeder Independent

Medium: mTeSR1

Matrix: Matrigel

Protocol WiCell Feeder Independent Protocol Passage Number p31

These cells were cultured for 30 passages prior to freeze, 4 of them in mTeSR1/Matrigel. WiCell adds +1 to the passage number at freeze so that the number on the vial best represents the overall passage number of the cells at thaw.

Date Vialed 15-June-2010

Vial Label WB0024 WA07 P27 LK 15 JUNE 10

Biosafety and Use Information Appropriate biosafety precautions should be followed when working with these cells. The end user is responsible for ensuring that the cells are handled and stored in an appropriate manner. WiCell is not responsible for damages or injuries that may result from the use of these cells. Cells distributed by WiCell are intended for research purposes only and are not intended for use in humans.

Product Information and Testing - Amended

©2010 WiCell Research Institute The material provided under this certificate has been subjected to the tests specified and the results and data described herein are accurate based on WiCell's reasonable knowledge and belief.  Appropriate Biosafety Level practices and universal precautions should always be used with this material.  For clarity, the foregoing is governed solely by WiCell’s Terms and Conditions of Service, which can be found at http://www.wicell.org/privacyandterms. 

Date of Lot Release Quality Assurance Approval

14-September-2010

1/3/2014

X AMCAMCQuality AssuranceSigned by:  

Page 1 of 1

WiCell Cytogenetics Report: 000033445555

WWIISSCC 99448888

Report Date: August 05, 2010

Case Details:

Cell Line: WWAA0077--WWBB00002244((99448888))

Passage #: 3322

Date Completed: 88//55//22001100

Cell Line Gender: FFeemmaallee

Investigator: WWiissccoonnssiinn IInntteerrnnaattiioonnaall SStteemm CCeellll BBaannkk

Specimen: hhEESS oonn MMaattrriiggeell

Date of Sample: 77//2233//22001100

Tests,Reason for: WWBB TTeessttiinngg

Results: 4466,,XXXX CCoommpplleetteedd bbyy MMSS,, CCGG((AASSCCPP)),, oonn 88//33//22001100

RReevviieewweedd aanndd iinntteerrpprreetteedd bbyy , PPhhDD,, FFAACCMMGG,, oonn 88//55//22001100

Interpretation: NNoo aabbnnoorrmmaalliittiieess wweerree ddeetteecctteedd aatt tthhee ssttaatteedd bbaanndd lleevveell ooff rreessoolluuttiioonn..

Cell: SS0022--0066

Slide: 55--77

Slide Type: KKaarryyoottyyppiinngg

# of Cells Counted: 20

# of Cells Karyotyped: 3

# of Cells Analyzed: 8

Band Level: 450-525

Results Transmitted by Fax / Email / Post Date:__________________________ Sent By:____________________________ Sent To:_______________________ QC Review By: ______________________ Results Recorded: ______________