G. Torzillo Istituto per lo Studio degli Ecosistemi

Sede di Firenze

Produzione di Idrogeno mediante microorganismi fotosintetici.

Energia e idrogeno: Le esperienze e le strategie europee. Il nuovo bando

energia del 7PQ. Le iniziative in Italia. Milano, 12 luglio, 2012

- Gaffron and Rubin, 1942

Metabolic pathways in Chlamydomonas reinhardtii

Le nostre principali attività di ricerca svolte nell’ambito

del progetto Hydrobio (FISR, MIUR, 2004-2009), EBH2

(Regione Toscana)

1) Selezione di ceppi di Chlamydomonas reinhardtii con elevati tassi di produzione di H2

2) Ottimizzazione delle condizioni colturali

3) Verifica all’aperto in fotobioreattori

PsbA gene – D1 protein

(Courtesy of U. Johanningmeier)

D1

294 153

Chlamydomonas reinhardtii mutants screened for the H2 production at ISE-FI

1) Photobioreactor;2) Intermediate bottle for gas-liquid conversion;3) Liquid-accumulating bottle; 4) Electronic balance; 5) PC with interface for data recording and storage; 6) - 7) - 8) Probes: Temp, pH, Eh; pO2; PAM-2100; 9) Magnetic mixing system.

a) b)

c) d)

Produzione di H2 mediante batteri fotosintetici

Brodi colturali arricchiti con acqua di vegetazione, quale sottoprodotto

dell’industria olearia , sono stati utilizzati per la produzione di energie verdi

Brodi colturali arricchiti con acqua di scarico, sono stati utilizzati per la

produzione d’idrogeno. In collaborazione CNR-ISE- UNIFI ( Prof Roberto

De Philippis).

(8 photons)

237 KJ/mole H2 x 2 moles

(209KJ/ mole quanta in PAR) x (8 moles of photons) PE = = 28.3%

28.3 x 0.45 = 12.7% (of solar light)

2H2O O2 + 4 e- + 4H+ 2H2

An ultimate potential objective might be to increase BioHydrogen

production as close as possible to about 600,000 m3 ha-1 y-1, which

represents solar light conversion efficiency to H2 of about 10% in sunny

areas.

H2ase

Recenti pubblicazioni

1) Torzillo G., Scoma A., Faraloni C., Ena A., Johanningmeier U. (2009). Increased hydrogen photoproduction by means of a sulfur-deprived Chlamydomonas reinhardtii D1 protein

mutant. Int J Hydrogen Energy: 34: 4529-4536.

2) Giannelli L., Scoma A., Torzillo G. (2009). Interplay between light intensity, chlorophyll concentration and culture mixing on the hydrogen production in sulfur-deprived C. reinhardtii

cultures grown in laboratory photobioreactors. Biotechnol Bioeng104: 76-90.

3) Faraloni C., Torzillo G. (2010) Phenotypic characterization and hydrogen production in Chlamydomonas reinhardtii QB binding D1 protein mutants under sulphur starvation: changes

in chlorophyll fluorescence and pigment composition. J Phycol, 46: 788-799.

4) Torzillo G. Giannelli L., Martinez-Roldan A.J., Verdone N., De Filippis P., Scarsella M., Bravi M. (2010). Microalgal culturing in thin-layer photobioreactors. Chem Eng Trans , 20: 265-

270.

5) Torri C., Samorì C., Adamiano A., Fabbri D., Fartaloni C., Torzillo G. (2011). Preliminary investigation on the production of fluels and bio-char from Chlamydomonas reinhardtii

biomass residue after bio-hydrogen production. Biotechl. Biores. (in press) Doi 10.1016/j.biortech.2011.01.064.

6) Faraloni C., Ena A., Pintucci C., Torzillo G. (2011). Enhanced hydrogen production by means of sulfur-deprived Chlamydomonas reinhardtii cultures grown in pre-treated olive mill

wastwater. Int. J. Hydrogen Energy 36: 5920-5931.

7) Scoma A., Giannelli L Faraloni C., Torzillo G. (2011). Outdoor H2 production in a 50-liter tubular photobioreactor by means of a sulfur-deprived culture of the microalga

Chlamydomonas reinhardtii . J. Biotechnol. (in press). Doi: 10.1016/j.jbiotec.2011.06.040

8) Scoma A., Giannelli L Faraloni C., Torzillo G. (2011) Sustained H2 production in a Chlamydomonas. reinhardii D1 protein mutant. J. Biotechnol. (in press). Doi:

10.1016/j.jbiotec.2011.06.019

9) Scarsella. M., Torzillo G., Cicci A., Belotti G., De Filippis P., Bravi M. (2011). Mechanical stress tolerance of two microalgae. Process Biochemistry (in press). Doi:

10.1016/j.procbio.2011.07.002

10) Carlozzi P. (2009). The effect of irradiance growing on hydrogen photoevolution and on the kinetic growth in Rhodopseudomonas palustris, strain 42OL. Int. J. Hydrogen Energy,

34(19): 7949-7958.

11) Carlozzi P., Lambardi M. (2009). Fed-batch operation for bio-H2 production by Rhodopseudomonas palustris (strain 42 OL). Renewable Energy, 34: 2577-2584.

12) Carlozzi P., Buccioni A., Minieri S., Pushparaj B., Piccardi R., Ena A., Pintucci C. (2010). Production of bio-fuels (hydrogen and lipids) through a photofermentation process. Biores.

Technol. 101: 3115-3120.

13) Carlozzi P, Scoma A, Pintucci C, Ena A. (2010). Co-production of bioH2 and biomasses rich in oil from two Rhodopseudomonas palustris strains: 42OL and 6A. In Clean Energy:

Resources, Production and Developments. Ed: Aiden M. Harris, Nova Science Publishers Inc, New York, Chap. 13, pp. 163-178, ISBN: 978-1-61761-509-2

14) Carlozzi P., Pintucci C., Piccardi R., Buccioni A., Minieri S., Lambardi M. (2010). Green energy from Rhodopseudomonas palustris grown at low to high irradiance values, under fed-

batch operational conditions. Biotechnol. Lett. 32: 477-481.

15)Rodolfi L, Chini Zittelli G, Bassi N, Padovani N, Biondi N, Bonini G, Tredici M R (2009). Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in

a low-cost photobioreactor. Biotechnol Bioeng 102: 100-112.

16)Tredici M.R., Chini Zittelli G., Rodolfi L. (2010). “Photobioreactors”. In: Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, Vol 6. Flickinger

M.C. (ed). J. Wiley & Sons Inc., New York, pp. 3821-3838.

17) Bondioli P., Della Bella L., Rivolta G., Casini D., Prussi M., Chiaramonti D., Chini Zittelli G., Bassi N., Rodolfi L., Tredici M.R. (2010). Oil production by the marine microalga

Nannochloropsis sp. F&M-M24. Proc. of 18th Europ. Biomass Conf., 3-7 May, Lyon, France. pp. 538-541. ISBN 978-88-89407-56-5; DOI: 10.5071/18thEUBCE2010-VP1.3.53

18) Chini Zittelli G., Rodolfi L., Bassi N., Tredici M.R. (in press) Photobioreactors for microalgae biofuel production. In: "Algae for Biofuels and Energy" (eds M. Borowitzka and N.

Moheimani).

19)Bondioli, L. Della Bella, G. Rivolta, G. Chini Zittelli, N. Bassi, L. Rodolfi, D. Casini, M. Prussi, D. Chiaramonti, M.R. Tredici. Oil production by the marine microalgae Nannochloropsis

sp. F&M-M24 and Tetraselmis suecica F&M-M33. Bioresource Technology (submitted 12.12.2011).

20) Adessi, A., Torzillo G., Baccetti E., De Philippis R. (2012) Susstained outdoor H2 production with Rhodopseudomonas palustris cultures in a 50 L tubular photobioreactor. Int. J. of

Hydroge energy;

21) Carlozzi P. (2012). Hydrogen photoproduction by Rhodopseudomonas palustris 42OL cultured at high irradiance under a semi-continuous regime. J Biomed Biotechnol (in press)

22) Samori C Torri C, Fabbri D, Falini, Faraloni et al., (2012) ChemSus Chem. (in press)

23) Torzillo G. Seibert (2012) Hydrogen production from Microalgae In Richmond ed. 2012 (in press).

24) Torzillo G., Faraloni C., Giannelli L. (2012) Biotechnology of hydrogen production with the microalga C. reinhardtii (The Science of Algal Fuels ( Gordon R., Seckbach eds), 2012

AN IMPLEMENTING AGREEMENT OF THE

INTERNATIONAL ENERGY AGENCY

H Y D R O G E N I M P L E M E N T I N G A G R E E M E N T

Subtasks 2010-2015

A. Bio-inspired Systems

B. Dark BioHydrogen Fermentation Systems

C. Basic studies for Light-Driven BioHydrogen

Production

D. Biological Electrochemical Systems

E. Overall Analysis

A collaborative research and development (R,D&D) program

created in 1977 on a task-shared “bottom-up” basis

“Bio-Inspired Hydrogen and BioHydrogen”

Michael Seibert, Operating Agent

IEA

Task-21:

MIUR (HYDROBIO)

Regione Toscana (EBH2; PROSEV;

OLIVARE)

Support

ENEL Brindisi (CO2-capture)

BANCHE (CRF; MPS)

EU: Oli-PHA- European project (2012-

2015) - AQUAGRIS; ALGINET,

H2 2H2O O2 +

GRAZIE

per

l’attenzione