protein self-assembly: · protein self-assembly: dynamic biomimetic materials state key laboratory...
TRANSCRIPT
State Key Laboratory of Supramolecular Structure and Materials Jilin University
超 分 子 结 构 与 材 料 国 家 重 点 实 验 室
Junqiu Liu
Nov 17 2015 Finland
Smart Enzyme Design and1048579Protein Self-assembly1048579Dynamic Biomimetic Materials1048579
State Key Laboratory of Supramolecular Structure and
Materials Chemistry College Jilin University China
Annual Seminar of Programmable Materials (OMA)
Outline
Enzyme Design
Recognation Interaction
High activity High stability
Protein Assembly
Recognition
Research motivation
Enzyme Design High activity High stability
High coordination
Enzyme Application Enzyme regulation
self-assemblyhydrogel
nanostuctures
Model
mimic
Accurate
mimicking
Static
state
Allosteric
state
Janne A Ihalainen etal Nature 2014
Self-assembly
Biomimetic
self-assembly
I Biomimetic enzymes
II Biomimetic light-harvesting
system
IIIBiomimetic muscle
I
II
III
Catalytic
group Recogniation
Fersht A R etal Nature 2000 403617
Isomerase
Phosphate Synthase
Biomimetic Enzyme Design
Leigh D A etal Science 2013 339 189
Dynamic Biomimetic Enzyme Design
Glutathione Peroxidase (GPx)
GPx catalyzed reaction
ROOH + 2GSH ROH + H2O + GSSG
GSH Recognition site
IEnzyme Design
Active site
GPx Active Site
Liu etal J Am Chem Soc 2005 127 11588
Liu etal J Am Chem Soc 2004 126 10556
Liu etal Angew Chem Int Ed 2009 48 2020
Liu Wulff J Am Chem Soc 2004 126 7452
Liu Wulff AngewChemInt Ed 2004 43 1287
Liu Wulff J Am Chem Soc 2008 130 8044
Liu etal J Am Chem Soc 2004 126 16395
Liu etal AngewChemInt Ed 2010 49 3920 Liu etal J Am Chem Soc 2013 135 10966
Liu etal AngewChemInt Ed 2013 525590
Liu etal ACSnano 2012 2013 2014 2015
Enzyme and Artificial Enzyme Design
Molecular System
Supramolecular System
Liu etal Angew Chem Int Ed 2014 13536
GS
T Ser9 TeCys9 Cys9
Te-GST
P Z 5 1 H 2 O 2
c a t a l y s t h y d r o p e r o x i d e a c t i v i t y
1
S e - G S T
G S T
N a t i v e G P X
0
3000
5 7 8 0
Te-GST 6000
Introduce catalytic site into binding site
Catalytic site
识别部位 Binding site
GPx activity of Telluro-GST
Liu et al Angew Chem Int Ed 2009482020 Chem Soc Rev 2011 40 1171
1 Design on natural protein
2 Smart enzyme design
Calcium ion switched selenoenzyme design
Luo Liu et al Angew Chem Int Ed 2014 13536 ndash13539
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Outline
Enzyme Design
Recognation Interaction
High activity High stability
Protein Assembly
Recognition
Research motivation
Enzyme Design High activity High stability
High coordination
Enzyme Application Enzyme regulation
self-assemblyhydrogel
nanostuctures
Model
mimic
Accurate
mimicking
Static
state
Allosteric
state
Janne A Ihalainen etal Nature 2014
Self-assembly
Biomimetic
self-assembly
I Biomimetic enzymes
II Biomimetic light-harvesting
system
IIIBiomimetic muscle
I
II
III
Catalytic
group Recogniation
Fersht A R etal Nature 2000 403617
Isomerase
Phosphate Synthase
Biomimetic Enzyme Design
Leigh D A etal Science 2013 339 189
Dynamic Biomimetic Enzyme Design
Glutathione Peroxidase (GPx)
GPx catalyzed reaction
ROOH + 2GSH ROH + H2O + GSSG
GSH Recognition site
IEnzyme Design
Active site
GPx Active Site
Liu etal J Am Chem Soc 2005 127 11588
Liu etal J Am Chem Soc 2004 126 10556
Liu etal Angew Chem Int Ed 2009 48 2020
Liu Wulff J Am Chem Soc 2004 126 7452
Liu Wulff AngewChemInt Ed 2004 43 1287
Liu Wulff J Am Chem Soc 2008 130 8044
Liu etal J Am Chem Soc 2004 126 16395
Liu etal AngewChemInt Ed 2010 49 3920 Liu etal J Am Chem Soc 2013 135 10966
Liu etal AngewChemInt Ed 2013 525590
Liu etal ACSnano 2012 2013 2014 2015
Enzyme and Artificial Enzyme Design
Molecular System
Supramolecular System
Liu etal Angew Chem Int Ed 2014 13536
GS
T Ser9 TeCys9 Cys9
Te-GST
P Z 5 1 H 2 O 2
c a t a l y s t h y d r o p e r o x i d e a c t i v i t y
1
S e - G S T
G S T
N a t i v e G P X
0
3000
5 7 8 0
Te-GST 6000
Introduce catalytic site into binding site
Catalytic site
识别部位 Binding site
GPx activity of Telluro-GST
Liu et al Angew Chem Int Ed 2009482020 Chem Soc Rev 2011 40 1171
1 Design on natural protein
2 Smart enzyme design
Calcium ion switched selenoenzyme design
Luo Liu et al Angew Chem Int Ed 2014 13536 ndash13539
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Recognition
Research motivation
Enzyme Design High activity High stability
High coordination
Enzyme Application Enzyme regulation
self-assemblyhydrogel
nanostuctures
Model
mimic
Accurate
mimicking
Static
state
Allosteric
state
Janne A Ihalainen etal Nature 2014
Self-assembly
Biomimetic
self-assembly
I Biomimetic enzymes
II Biomimetic light-harvesting
system
IIIBiomimetic muscle
I
II
III
Catalytic
group Recogniation
Fersht A R etal Nature 2000 403617
Isomerase
Phosphate Synthase
Biomimetic Enzyme Design
Leigh D A etal Science 2013 339 189
Dynamic Biomimetic Enzyme Design
Glutathione Peroxidase (GPx)
GPx catalyzed reaction
ROOH + 2GSH ROH + H2O + GSSG
GSH Recognition site
IEnzyme Design
Active site
GPx Active Site
Liu etal J Am Chem Soc 2005 127 11588
Liu etal J Am Chem Soc 2004 126 10556
Liu etal Angew Chem Int Ed 2009 48 2020
Liu Wulff J Am Chem Soc 2004 126 7452
Liu Wulff AngewChemInt Ed 2004 43 1287
Liu Wulff J Am Chem Soc 2008 130 8044
Liu etal J Am Chem Soc 2004 126 16395
Liu etal AngewChemInt Ed 2010 49 3920 Liu etal J Am Chem Soc 2013 135 10966
Liu etal AngewChemInt Ed 2013 525590
Liu etal ACSnano 2012 2013 2014 2015
Enzyme and Artificial Enzyme Design
Molecular System
Supramolecular System
Liu etal Angew Chem Int Ed 2014 13536
GS
T Ser9 TeCys9 Cys9
Te-GST
P Z 5 1 H 2 O 2
c a t a l y s t h y d r o p e r o x i d e a c t i v i t y
1
S e - G S T
G S T
N a t i v e G P X
0
3000
5 7 8 0
Te-GST 6000
Introduce catalytic site into binding site
Catalytic site
识别部位 Binding site
GPx activity of Telluro-GST
Liu et al Angew Chem Int Ed 2009482020 Chem Soc Rev 2011 40 1171
1 Design on natural protein
2 Smart enzyme design
Calcium ion switched selenoenzyme design
Luo Liu et al Angew Chem Int Ed 2014 13536 ndash13539
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Self-assembly
Biomimetic
self-assembly
I Biomimetic enzymes
II Biomimetic light-harvesting
system
IIIBiomimetic muscle
I
II
III
Catalytic
group Recogniation
Fersht A R etal Nature 2000 403617
Isomerase
Phosphate Synthase
Biomimetic Enzyme Design
Leigh D A etal Science 2013 339 189
Dynamic Biomimetic Enzyme Design
Glutathione Peroxidase (GPx)
GPx catalyzed reaction
ROOH + 2GSH ROH + H2O + GSSG
GSH Recognition site
IEnzyme Design
Active site
GPx Active Site
Liu etal J Am Chem Soc 2005 127 11588
Liu etal J Am Chem Soc 2004 126 10556
Liu etal Angew Chem Int Ed 2009 48 2020
Liu Wulff J Am Chem Soc 2004 126 7452
Liu Wulff AngewChemInt Ed 2004 43 1287
Liu Wulff J Am Chem Soc 2008 130 8044
Liu etal J Am Chem Soc 2004 126 16395
Liu etal AngewChemInt Ed 2010 49 3920 Liu etal J Am Chem Soc 2013 135 10966
Liu etal AngewChemInt Ed 2013 525590
Liu etal ACSnano 2012 2013 2014 2015
Enzyme and Artificial Enzyme Design
Molecular System
Supramolecular System
Liu etal Angew Chem Int Ed 2014 13536
GS
T Ser9 TeCys9 Cys9
Te-GST
P Z 5 1 H 2 O 2
c a t a l y s t h y d r o p e r o x i d e a c t i v i t y
1
S e - G S T
G S T
N a t i v e G P X
0
3000
5 7 8 0
Te-GST 6000
Introduce catalytic site into binding site
Catalytic site
识别部位 Binding site
GPx activity of Telluro-GST
Liu et al Angew Chem Int Ed 2009482020 Chem Soc Rev 2011 40 1171
1 Design on natural protein
2 Smart enzyme design
Calcium ion switched selenoenzyme design
Luo Liu et al Angew Chem Int Ed 2014 13536 ndash13539
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Catalytic
group Recogniation
Fersht A R etal Nature 2000 403617
Isomerase
Phosphate Synthase
Biomimetic Enzyme Design
Leigh D A etal Science 2013 339 189
Dynamic Biomimetic Enzyme Design
Glutathione Peroxidase (GPx)
GPx catalyzed reaction
ROOH + 2GSH ROH + H2O + GSSG
GSH Recognition site
IEnzyme Design
Active site
GPx Active Site
Liu etal J Am Chem Soc 2005 127 11588
Liu etal J Am Chem Soc 2004 126 10556
Liu etal Angew Chem Int Ed 2009 48 2020
Liu Wulff J Am Chem Soc 2004 126 7452
Liu Wulff AngewChemInt Ed 2004 43 1287
Liu Wulff J Am Chem Soc 2008 130 8044
Liu etal J Am Chem Soc 2004 126 16395
Liu etal AngewChemInt Ed 2010 49 3920 Liu etal J Am Chem Soc 2013 135 10966
Liu etal AngewChemInt Ed 2013 525590
Liu etal ACSnano 2012 2013 2014 2015
Enzyme and Artificial Enzyme Design
Molecular System
Supramolecular System
Liu etal Angew Chem Int Ed 2014 13536
GS
T Ser9 TeCys9 Cys9
Te-GST
P Z 5 1 H 2 O 2
c a t a l y s t h y d r o p e r o x i d e a c t i v i t y
1
S e - G S T
G S T
N a t i v e G P X
0
3000
5 7 8 0
Te-GST 6000
Introduce catalytic site into binding site
Catalytic site
识别部位 Binding site
GPx activity of Telluro-GST
Liu et al Angew Chem Int Ed 2009482020 Chem Soc Rev 2011 40 1171
1 Design on natural protein
2 Smart enzyme design
Calcium ion switched selenoenzyme design
Luo Liu et al Angew Chem Int Ed 2014 13536 ndash13539
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Fersht A R etal Nature 2000 403617
Isomerase
Phosphate Synthase
Biomimetic Enzyme Design
Leigh D A etal Science 2013 339 189
Dynamic Biomimetic Enzyme Design
Glutathione Peroxidase (GPx)
GPx catalyzed reaction
ROOH + 2GSH ROH + H2O + GSSG
GSH Recognition site
IEnzyme Design
Active site
GPx Active Site
Liu etal J Am Chem Soc 2005 127 11588
Liu etal J Am Chem Soc 2004 126 10556
Liu etal Angew Chem Int Ed 2009 48 2020
Liu Wulff J Am Chem Soc 2004 126 7452
Liu Wulff AngewChemInt Ed 2004 43 1287
Liu Wulff J Am Chem Soc 2008 130 8044
Liu etal J Am Chem Soc 2004 126 16395
Liu etal AngewChemInt Ed 2010 49 3920 Liu etal J Am Chem Soc 2013 135 10966
Liu etal AngewChemInt Ed 2013 525590
Liu etal ACSnano 2012 2013 2014 2015
Enzyme and Artificial Enzyme Design
Molecular System
Supramolecular System
Liu etal Angew Chem Int Ed 2014 13536
GS
T Ser9 TeCys9 Cys9
Te-GST
P Z 5 1 H 2 O 2
c a t a l y s t h y d r o p e r o x i d e a c t i v i t y
1
S e - G S T
G S T
N a t i v e G P X
0
3000
5 7 8 0
Te-GST 6000
Introduce catalytic site into binding site
Catalytic site
识别部位 Binding site
GPx activity of Telluro-GST
Liu et al Angew Chem Int Ed 2009482020 Chem Soc Rev 2011 40 1171
1 Design on natural protein
2 Smart enzyme design
Calcium ion switched selenoenzyme design
Luo Liu et al Angew Chem Int Ed 2014 13536 ndash13539
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Leigh D A etal Science 2013 339 189
Dynamic Biomimetic Enzyme Design
Glutathione Peroxidase (GPx)
GPx catalyzed reaction
ROOH + 2GSH ROH + H2O + GSSG
GSH Recognition site
IEnzyme Design
Active site
GPx Active Site
Liu etal J Am Chem Soc 2005 127 11588
Liu etal J Am Chem Soc 2004 126 10556
Liu etal Angew Chem Int Ed 2009 48 2020
Liu Wulff J Am Chem Soc 2004 126 7452
Liu Wulff AngewChemInt Ed 2004 43 1287
Liu Wulff J Am Chem Soc 2008 130 8044
Liu etal J Am Chem Soc 2004 126 16395
Liu etal AngewChemInt Ed 2010 49 3920 Liu etal J Am Chem Soc 2013 135 10966
Liu etal AngewChemInt Ed 2013 525590
Liu etal ACSnano 2012 2013 2014 2015
Enzyme and Artificial Enzyme Design
Molecular System
Supramolecular System
Liu etal Angew Chem Int Ed 2014 13536
GS
T Ser9 TeCys9 Cys9
Te-GST
P Z 5 1 H 2 O 2
c a t a l y s t h y d r o p e r o x i d e a c t i v i t y
1
S e - G S T
G S T
N a t i v e G P X
0
3000
5 7 8 0
Te-GST 6000
Introduce catalytic site into binding site
Catalytic site
识别部位 Binding site
GPx activity of Telluro-GST
Liu et al Angew Chem Int Ed 2009482020 Chem Soc Rev 2011 40 1171
1 Design on natural protein
2 Smart enzyme design
Calcium ion switched selenoenzyme design
Luo Liu et al Angew Chem Int Ed 2014 13536 ndash13539
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Glutathione Peroxidase (GPx)
GPx catalyzed reaction
ROOH + 2GSH ROH + H2O + GSSG
GSH Recognition site
IEnzyme Design
Active site
GPx Active Site
Liu etal J Am Chem Soc 2005 127 11588
Liu etal J Am Chem Soc 2004 126 10556
Liu etal Angew Chem Int Ed 2009 48 2020
Liu Wulff J Am Chem Soc 2004 126 7452
Liu Wulff AngewChemInt Ed 2004 43 1287
Liu Wulff J Am Chem Soc 2008 130 8044
Liu etal J Am Chem Soc 2004 126 16395
Liu etal AngewChemInt Ed 2010 49 3920 Liu etal J Am Chem Soc 2013 135 10966
Liu etal AngewChemInt Ed 2013 525590
Liu etal ACSnano 2012 2013 2014 2015
Enzyme and Artificial Enzyme Design
Molecular System
Supramolecular System
Liu etal Angew Chem Int Ed 2014 13536
GS
T Ser9 TeCys9 Cys9
Te-GST
P Z 5 1 H 2 O 2
c a t a l y s t h y d r o p e r o x i d e a c t i v i t y
1
S e - G S T
G S T
N a t i v e G P X
0
3000
5 7 8 0
Te-GST 6000
Introduce catalytic site into binding site
Catalytic site
识别部位 Binding site
GPx activity of Telluro-GST
Liu et al Angew Chem Int Ed 2009482020 Chem Soc Rev 2011 40 1171
1 Design on natural protein
2 Smart enzyme design
Calcium ion switched selenoenzyme design
Luo Liu et al Angew Chem Int Ed 2014 13536 ndash13539
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Liu etal J Am Chem Soc 2005 127 11588
Liu etal J Am Chem Soc 2004 126 10556
Liu etal Angew Chem Int Ed 2009 48 2020
Liu Wulff J Am Chem Soc 2004 126 7452
Liu Wulff AngewChemInt Ed 2004 43 1287
Liu Wulff J Am Chem Soc 2008 130 8044
Liu etal J Am Chem Soc 2004 126 16395
Liu etal AngewChemInt Ed 2010 49 3920 Liu etal J Am Chem Soc 2013 135 10966
Liu etal AngewChemInt Ed 2013 525590
Liu etal ACSnano 2012 2013 2014 2015
Enzyme and Artificial Enzyme Design
Molecular System
Supramolecular System
Liu etal Angew Chem Int Ed 2014 13536
GS
T Ser9 TeCys9 Cys9
Te-GST
P Z 5 1 H 2 O 2
c a t a l y s t h y d r o p e r o x i d e a c t i v i t y
1
S e - G S T
G S T
N a t i v e G P X
0
3000
5 7 8 0
Te-GST 6000
Introduce catalytic site into binding site
Catalytic site
识别部位 Binding site
GPx activity of Telluro-GST
Liu et al Angew Chem Int Ed 2009482020 Chem Soc Rev 2011 40 1171
1 Design on natural protein
2 Smart enzyme design
Calcium ion switched selenoenzyme design
Luo Liu et al Angew Chem Int Ed 2014 13536 ndash13539
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
GS
T Ser9 TeCys9 Cys9
Te-GST
P Z 5 1 H 2 O 2
c a t a l y s t h y d r o p e r o x i d e a c t i v i t y
1
S e - G S T
G S T
N a t i v e G P X
0
3000
5 7 8 0
Te-GST 6000
Introduce catalytic site into binding site
Catalytic site
识别部位 Binding site
GPx activity of Telluro-GST
Liu et al Angew Chem Int Ed 2009482020 Chem Soc Rev 2011 40 1171
1 Design on natural protein
2 Smart enzyme design
Calcium ion switched selenoenzyme design
Luo Liu et al Angew Chem Int Ed 2014 13536 ndash13539
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
2 Smart enzyme design
Calcium ion switched selenoenzyme design
Luo Liu et al Angew Chem Int Ed 2014 13536 ndash13539
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
ATP-switched smart selenoenzyme design
A) B)
Adenylate kinase
Anti-mitochondrial damage model
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Hou Liu etal ACSnano 2012 6 8692
Design an
active site
Self-assembly
3 Enzyme Design on Protein Assemblies
Enzyme design on natural protein nanotube
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
4 Enzyme design by self-assembly
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Catalysts Substrate Relative
activity
PhSeSePh TNB 1
NT(12) TNB 0
NT (12bSe) TNB 67080
NT (12bTe) TNB 254580
NT (12bTe2c) TNB 305000
GPx activity
Enzyme design on giant nanotubes
with molecular imprinting
Liu etal AngewChemInt Ed 2010493920
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Self-Assembly
Construct of the thermosensitive nanoenzyme
Self-Assembly
Thermosensitive nanostructures construct between the Nanotubes and Vesicle-like Structures
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Turn on off enzyme activity
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
5 Enzyme design on self-assembled peptides
Amphiphilic peptide self-assembly
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Liu Zou etal ACSnano 2014 11715
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
6 Mimicking Helice Structure
Dong Liu et al Angew Chem Int Ed 2015 3140
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Liu Shen etal Chem Soc Review 2010 In press
Liu etal Chem Soc Rev
2011 40 1171
(Invited review
Inside Front Cover)
Top ten accessed articles
Liu etal Chem Soc Rev
2012 41 7890
(Invited review)
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Kcat K uncat
N N H
1
N N H
N N H 2
N H 2
2
N N H
N
H 2 N
N N H
3
100
4040
410000
Liu Wulff J Am Chem Soc 2008 130 8044 (Top 10 Articles Published in the Same Domain No1)
7 Stablization of Transition-state by
Molecularly Imprinting
功能单体
TS Recognition Site
Catalytic sites in TS site
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Wulff Liu
Acc Chem Res 201245239
(Invited reviewFront cover )
Artificial active site
Energy levels in catalysis
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
protein
How to control the protein-protein interaction
Protein
Interaction face
How to control the orientation of the protein assembly
IIProtein Assembly
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
1048579 D Baker etal Science 2012 336 1175
Protein Self-Assembly with Atomic Level Accuracy
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Enzyme Design on Protein assembly in vivo
Tezcan F A etal Science 2014 346 1525
With in vivo activity
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
1 Protein Assembly by Host- guest Interaction
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Enzyme functionality
Liu etal AngewChemInt Ed 2013 525590
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Unpublished results
Control the orientation
of the protein assemblies Host-guest interaction
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
2 Accurate Control of Protein Self-assembly
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
noncovalent
interaction
network
Metal ion
site
equiv
Metal coordination site
1
2
4
3
5
Self-assembly nanoring
a b
c
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
42
nm
GST Dimer
Liu etal J Am Chem Soc 2013 135 10966 (Highlight by Nature China)
All rings
Diameter of 367nm
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Control of the size of
nanorings
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Unpublished results
4 Control the dynamic motion
Ca2+-
binding
protein
Protein nanospring
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
3-10nm
+ +
+
+
+ +
+ +
+
3nm
11nm
_ _
_ _
_ _
_
_
_ _
_ _
_ _
Liu etal ACS Nano 2014 8 3743
5 Charge Drived Protein Assembly
Mimicking light-harvesting system by Quantum Dots
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Self-Assembly Induced by ldquoSoft Nanoparticlesrdquo
Liu etal ACS Nano 2015 9 5461
Enzyme coorperation on
protein assembly
GPx
SOD
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Self-Assembly of Cricoid Proteins Induced by ldquoSoft Nanoparticlesrdquo
Mimicking light-harvesting system
Liu etal ACS Nano 2015 revised
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
201
401
501
201
401
501
201
401
Zn2+ protein
501
201
401
IIIDesign of 2D Protein Assemblies
1 Zn2+binding models through metal
coordination
AFM images of assemblies
Smac protein
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
3 Enzyme Regulation
Protein Hydrogel Self-Healing
Glucose oxidase
(GOX)
Catalase
(CAT)
Enzymes
cooperation
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Self-healing behavior of protein hydrogel
Liu etal AngewChemInt Ed 2014 53 9343-9346 (Hot paper)
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Enzymes Design
bull Host-Guest artificial enzymes
bull Suparmolecular artificial enzymes
bull Mechanism study of the nature of enzyme catalysis
bull Cooperation between recognition and catalysis
Protein Assembly
bull Enzyme design and artificial enzymes
bull Protein assembly structure
bull Light-harvesting system
Summary
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Acknowledgment
The National Natural Science Foundation
The High Technology Research Development Plan
The Major State Basic Research Development Program
Financial support
Collaborators
Prof Jiacong Shen Prof Guimin Guo
Prof August Bock Lehrstuhl fur Mikrobiologie der Universitat Munchen
Prof Louis A Pete Silks Los Alamos National Laboratory
Prof Gunter Wulff Institute of Organic and Macromolecular Chemistry
Germany
Prof Xi Zhang Tsinghua University China
Dr Zheyuan Dong Huijun Yu Shizhong Mao
PhDStudent Jing Li Xin Huang Xiaoman Liu Yan Ge Xiangqiu Li
Ping Wu Zhiming ZhangXiaoguang Wang Yuetong Ji
MsStudant Chunqiu ZhangYongguo Wang Yong Tang Zhenhui Qi
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Liursquos Research Group (刘俊秋教授研究组)
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室
Key Laboratory for Supramolecular
Structure and Materials
Thank you
吉林大学超分子结构与材料教育部重点实验室