qualityfoodsafetyproductfolderen_11-2012

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QUALITY & FOOD

SAFETY SOLUTIONS

Services and products for quality management:

- DMDnutrient media for the microbiological detection of beverage-spoiling micro-organisms

- Analytical Services, accredited laboratory for product analysis

- Technical Services, specialist advice about quality management

WE BRING IDEAS TO L IFE.


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Premium beverages require a constantly high level of product and

process safety.

Standardised quality assurance of the products, combined with a

continuous optimisation process, is a central component of modern

quality management in accordance with DIN EN ISO 9001, IFS and BRC.

As well as product analysis, it includes regular inspection of all process

steps relevant to quality. The influential factors are measured and

analysed so that the findings gained can be applied as quality assurance

measures.

Our customers recognize Dhler as a supplier of professional services

and products (DMD) which are indispensable for quality assurance,

especially in breweries and the non-alcoholic beverage industry.

DMD

Under the brand Dhler Microsafety Design (DMD) Dhler offers

microbiologically selective nutrient media for almost all sectors of

the beverage and brewing industry.

They are easy to use and achieve fast, and above all reliable, results.

Almost all are ready-to-use and can be deployed without additional

costs or time-consuming treatment measures.

ANALYTICAL & TECHNICAL SERVICES

Dhler offers quality assurance services. These include analyses of

your products in our laboratories and specialist advice from our

technical field service, always giving you an independent

perspective in all questions relating to quality assurance in

production.

QUALITY & FOOD

SAFETY SOLUTIONS


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Dhler Microsafety Design

Simple Fast Safe

Dhler has been established as a supplier of microbiological nutrient

media in the international beverage industry for more than 25 years.

The media systems are recommended by ministries and institutes and

even quoted in the standards authority benchmarks. This international

acceptance is why our customers put their trust in the quality of the

DMDproducts.

DMD benefits:ready-to-use nutrient mediasimple applicationselective propertiesfast, safe and reliable results

In close cooperation with

professors from internationally

renowned universities, institutes

and laboratories, we develop products tailored to market demands

and strive for innovative solutions which fulfil your requirements.

DMDCULTURES

In the soft drink and juice industry for the detection

of harmful moulds, yeast and bacteria p. 4 5SSL-Broth for enrichment

OFS-Agar

TransFastSystem

- TransFast-Broth for enrichment

- TransFast-Gel

In breweries for the detection of all relevant beer

and wine spoiling bacteria p. 6 7

NBB-Agar (NBB-A)

NBB-Broth (NBB-B)

NBB-Concentrate (NBB-C)

NBB-Powder (NBB-P)

In the juice industry for the detection of

Alicyclobacillus p. 8

BAT-Agar

BAT-Broth

Guaiacol detection kit

For the detection of water-specifi c germs p. 8

Nutrient Agar in accordance with DEV

LMC, concentrated lactose-pepton-broth in

accordance with DEV

For the validation of aseptic fi lling lines p. 9

AVM Aseptic Validation Medium

For the hygienic monitoring in the fi lling area p. 9

NBB-B Enrichment Medium (NBB-B-AM)

Total view p. 10


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OFS-AGAR (pH 5.0 +/- 0.1)

For the detection of yeast, moulds, acetic acid and

lactic acid bacteria

APPLICATION

- analysis of ready-to-drink beverages and rinsing water

samples in the non-alcoholic beverage, dairy and pressing

sectors as nutrient media from membrane filters

- in the pour plate method

- swabs from a wide range of samples

BENEFITS

- reliable results after 3 days

- easy to use

- very good quantitative and qualitative evaluation of results

- some results can be evaluated macroscopically

SSL-BROTH (pH 5.0 +/- 0.1)

For the enrichment of beverage-spoiling bacteria

APPLICATION

- for the enrichment of beverage-spoiling bacteria

- can be used with filtered and non-filtered drinks

- nutrient media for swab samples

BENEFITS

- inoculation of the sample after just 1-2 days

- ready-made easy to use media

- provides clear results on trace infections in combination

with OFS agar

OFS-Agar, SSL-Broth, TransFast-

System for soft drinks

The Dhler nutrient media for non-alcoholic beverages allow the user

to detect harmful micro-organisms in the entire beverage production

process chain reliably and quickly.

The cultures work extremely selectively. Only the bacteria responsible

for the spoiling find the ideal conditions to grow quickly. Almost all

nutrient media are ready for use, and additional mixing or sterilisation

is not necessary.

IN THE SOFT DRINK AND JUICE INDUSTRY FOR THE DETECTION OF

HARMFUL MOULDS, YEAST AND BACTERIA


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TransFastSystem an evaluation

within seconds

With TransFast, Dhler has succeeded in developing a completely new

microbiological detection method in terms of how fast results are

achieved.

While classic media are poured into Petri dishes as agar, the carrier

system in TransFastis a transparent gel. The beverage sample, which

is usually enriched in advance, is placed with the gel into a transparent

tube and incubated. Depending on the bacterial concentration, first

results can be seen after just 24 hours.

Another innovation is an incubator with interior lighting. The direct

illumination of the transparent samples allows any possible

contamination to be assessed in a fraction of a second. Each beverage-

spoiling micro-organism, whether it be bacteria, yeast or mould, has a

unique macroscopic appearance.

There is no need for the time-consuming use of Petri dishes!

Just turn on the light and you will be able to assess the overall condition

of the samples immediately.

TRANSFAST-BROTH (pH 6.1 +/- 0.1)

For the enrichment of beverage-spoiling bacteria

APPLICATION

- can be used with compounds and ready-to-drink beverages

- optimal in combination with TransFast-Gel

BENEFITS

- inoculation of the sample after just 1-2 days

- high sample volume ensures statistical evaluation

- ready-made easy to use media

TRANSFAST-GEL (pH 4.3 +/- 0.1)

For the detection of yeast, moulds, acetic acid and

lactic acid bacteria

APPLICATION

- analysis of ready-to-drink beverages, beverage compounds,

rinsing water enriched with TransFast-Broth

- preparation of the sample in sterile TransFast-Tubes

BENEFITS

- evaluation of the samples in seconds due to uncomplicated

screening

- ready-made media replace time-consuming liquefying of

the agar

- first results after 24 hours depending on the initial

contamination

- selective on beverage-spoiling micro-organisms


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NBB

Under the NBBbrand, we offer nutrient media for the microbiological

detection of beer-spoiling micro-organisms in breweries, which are

characterised by being easy and quick to use and by their excellent

selectivity.

The microbiological complexity of beer demands high standards of an

extremely sensitive culture for the detection of beer-spoiling bacteria.

The result is a very selective and fast-working medium: NBB. Depending

on the sample types in the brewery, NBB-Agar (NBB-A), NBB-

Broth (NBB-B), NBB-Concentrate (NBB-C) and NBB-B enrichment

media (NBB

-B-AM) are used. This means that the entire spectrum ofsamples which are taken in a brewery including turbid and yeast-

containing beers are covered. The precise ratio of special growth

materials and inhibitors allows the development of an ideal medium

with outstanding selective properties for harmful bacteria in beer.

Brewers yeast and harmless secondary flora are largely inhibited. NBB

is also suited for detecting pests in the wine industry.

NBB-BROTH (NBB-B) (pH 5.8 +/- 0.1)

Analysis of yeast samples and cloudy, yeast-containing beer samples

APPLICATION

- as nutrient medium for membrane filters

- pre-enrichment of beer samples, even for methods in

molecular biology (VIT, PCR)

BENEFITS

- broth works selectively, specifically for beer

- reliable results after just 3 days

- fast macroscopic evaluation using incorporated

indicator colour- easy to use

- also available in pre-filled test tubes for immediate use

NBB-AGAR (NBB-A) (pH 5.8 +/- 0.1)

Analysis of fi ltered beers

APPLICATION

- as nutrient medium for membrane filters


- swabs from a wide range of samples from the brewery

(beer samples, water samples)

BENEFITS

- agar works selectively, specifically for beer- reliable results after just 5 days

- fast evaluation using incorporated indicator colour

- very good quantitative and qualitative evaluation of results

- easy to use

IN BREWERIES FOR THE DETECTION OF

ALL RELEVANT BEER AND WINE SPOILING BACTERIA


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Alternative to the ready-to use products NNB is also available as a

powder. The essential growth materials, the inhibitors and the indicatorcolour are incorporated.

For the making of the agar or the broth, own bier may be used to obtain

a highly specific medium.

NBB-CONCENTRATE (NBB-C)

(pH 6.4 +/- 0.1)

Analysis of cloudy and yeast-containing beer

samples and yeast samples

APPLICATION

- as additive in the shelf life test samples

- pre-enrichment of beer samples, even for methods in


BENEFITS

- adjustable selectivity of the culture using different

concentrations and your own beer- records the full spectrum of beer-spoiling micro-organisms,

from obligate to indicator bacteria, depending on the

concentration used


NBB-POWDER (NBB-P)

For the preparation of NBB-Agarand Broth with own beer

APPLICATION

- use is analogous to NBB-B when the product is dissolved

in demineralised water and decarbonated beer

- in addition with agar-agar use is analogous to NBB-A when

dissolved in demineralised water and decarbonated beer

BENEFITS

- adaptation of the proven NBBproperties to the selectivity

of your beers

- small pack size makes it especially suitable for use withminimal cooling options


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BAT-Agar, BAT-Broth and

Guaiacol detection kit

Alicyclobacillus can damage beverages by the formation of an off-flavour

(guaiacol, 2,6-dibromphenol, 2,6 dichlorophenol), although only

A. acidoterrestris, A. acidiphilus and A. herbarius have shown

the potential for forming off-flavours up to now. The detection of

Alicyclobacillus alone therefore does not provide conclusive information

on the extent or risk posed of damaging a beverage. Instead, the risk

potential for the formation of off-flavours must be verified using the

Guaiacol detection kit, an enzyme test.

Nutrient Agar, LMC-Concentrate

in accordance with DEV

The requirements of the administrative bodies expect from beverage

producers that process water meets the quality criterion of drinking

water. This applies both for water processed in the product and for

water used for rinsing and cleaning.

Our nutrient agar in accordance with DEV allows the user to detect the

total amount of colonies in water and in ready-to-drink beverages.

With the LMC-concentrate, we are offering a product for the fast and

uncomplicated detection of E.coliand coliform bacteria.

IN THE JUICE INDUSTRY FOR THE

DETECTION OF ALICYCLOBACILLUS SPP.

FOR THE DETECTION

OF WATER-SPECIFIC GERMS

BAT-AGAR, BAT-BROTH (pH 4.0 +/- 0.1)

AND GUAIACOL DETECTION KIT

APPLICATION

-BAT-Broth is used for the enrichment of samples for the

detection of Alicyclobacillus

-BAT-Agar is used for the classic detection of Alicyclobacillus

-the Guaiacol detection kit is an enzyme test to determinethe potential for the formation of off flavours through

Alicyclobacillus

BENEFITS

-determination of Alicyclobacillus in accordance withIFU method No.12

-reliable, sensitive and economical method of analysis forAlicyclobacillus, even in complex products

-reliable results after just 2 days

-very easy to use

AGAR NUTRIENT IN ACCORDANCE

WITH DEV (pH 7.2 +/- 0.1)

APPLICATION

- determination of the total viable count in water, food and

beverage and rinsing water samples

- nutrient medium for membrane filters


- swabs from a wide range of samples

BENEFITS- nutrient medium for water analysis in accordance with the German

uniformity process (DEV) and drinking water regulations

- reliable results after just 1-2 days

- simple to use

LMC-CONCENTRATE AS PER DEV (pH 7.2 +/- 0.1)

APPLICATION

- detection of E.coliand coliform bacteria in water

BENEFITS

- samples are taken directly in the bottle pre-prepared with

nutrient media and are also incubated in this


- fast evaluation using incorporated indicator colour


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AVM Aspetic Validation Medium

Aseptic filling lines must be tested for functionality after installation. It is

recommended to use a standardised nutrient solution (AVM) which is

filled aseptically under production conditions, just like a finished

product. The filled bottles are incubated for 5 days and assessed visually

checking the turbidity from contaminations.

In addition, the AVMoffers the option of checking the performance of

the equipment components as a challenge test by selective inoculation

with a germ suspension.

NBB-B Enrichment Medium

The risk of secondary contamination by beverage-spoiling micro-

organisms is highest in the filling and packaging area. It is therefore

even more important to know the current hygiene status of the plant at

all times. As part of an analysis of weak spots, critical areas in filling

lines are defined and regularly sampled using streak swabs. The

incorporated indicator colour in NBB-B-AM makes a positive result

turn from red to yellow. If the percentage of positive samples remains

above a safety threshold of 20% for a longer period, this indicates

persistent biofilms in which harmful organisms and other problematic

bacteria will sooner or later be able to implant and adapt. A thoroughgeneral cleaning must be undertaken immediately or the risk of

secondary infections grows due to the generation of aerosols.

FOR THE VALIDATION

OF ASEPTIC FILLING LINES

FOR THE HYGIENIC MONITORING

IN THE FILLING AREA

AVM ASEPTIC VALIDATION MEDIUM

APPLICATION

-validation of aseptic filling lines

-requalification after maintenance or reconstruction work on

the line

BENEFITS

-AVMis available as a ready-to-use solution, as a 5-fold

concentrate or as a powder-flexible pH-settings enables the application from acid to

neutral range

-reliable results after just 5 days

-can detect trace or scattered contamination

-simple visual assessment through formation of cloudiness

in result

-evaluation of the entire fill volume without the need for

statistical multiplication

-standardised tool as part of certification in accordance with

standards such as DIN EN ISO 22000, BRC, IFS and the

HACCP system

NBB-B ENRICHMENT MEDIUM (NBB-B-AM)

(pH 5.8 +/- 0.1)

APPLICATION

-as nutrient medium for swab samples from filling andproduction

-for the detection of indicator bacteria for the prevention ofsecondary contamination

-for all beverage-producing plants

BENEFITS

-the indicator bacteria at the source of any secondaryinfection are detected clearly and quickly, especially in the

filling area

-as macroscopic evaluation using incorporated indicator

colour

-easy to use

-the hygiene standard of a plant can be permanentlymonitored and controlled using periodic analyses of weak

spots

-pre-enrichment of beer samples, even for methods in



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DescriptionpH

(+/- 0,1)

IncubationReference

time [d] temp. [C]Page

mplex agar for the enumeration of the total plate count, e.g. in water as per DEVutsche Einheitsverfahren - German standard published by DIN)

7,2 2 27 29 DIN

for water analysis Minera l- und

Tafelwasser VO2 or 1 20 or 37

8

old concentrated selective broth for the analysis of E.Coli and Coliforms in water samples as perV (Deutsches Einheitsverfahren - German standard published by DIN)

7,2 2 37 DIN, ISO,

TrinkWV,

Mineral- u.Tafelwasser VO

8

ective agar for the quantitative detection and isolation of beer or wine spoilers in filtered beer or

e used for membrane filtration or pour plate method, air sampling

5,8 5 7 27 29 MEBAK

(Mitteleuro-pische Brau-technischeAnalysen-kommision)

ective liquid enrichment medium for the qualitative detection of beer and wine spoilers in culture

st

5,8 3 5 27 29

bes filled with NBB-Broth for the direct use 5,8 3 5 27 29

wder for the preparation of NBB-Agar and NBB-Broth

ective liquid enrichment medium, concentrated, for the detection of beer spoilers in unfiltered beer 6,4 5 10 27 29

ective agar for the quantitative detection of beer spoilers in filtered beer 5,5 7 27 29

ective liquid enrichment medium for the qualitative detection of beer spoilers in culture yeast 5,6 7 27 29

ar for isolating and determining colony count of yeast and moulds 5,6 3 5 27 29

ar for the detection of wild yeast 4,8 2 5 27 29

ar for the differentiation of culture and wild yeast 5,8 2 5 27 29

ar for the cultivation of fastidious bacteria (mesophilic and thermophilic) 7,5 1 3 27 29

6 7

ective agar for the quantitative detection and isolation of beverage spoilers used for membraneation or pour plate method

5,0 2 3 27 29 IFU

(InternationalFederation ofFruit Juice

Producers)

ective liquid enrichment medium as prestage for trace contamination 5,0 1 2 27 29

ective liquid enrichment medium as prestage for trace contamination with higher pH, even formples with lower pH

6,1 1 2 27 29

ective and transparent liquid Gel for the qualitative detection of beverage spoilers 4,3 1 2 27 29

ective agar for the quantitative detection and isolation of Alicyclobacilllusspp. 4,0 2 27 29 IFU

(InternationalFederation ofFruit JuiceProducers)

ective liquid enrichment medium as prestage for trace contamination 4,0 2 27 29

ymatic detection of guaiacol forming Alicyclobacilli for assessing the risk potential of off-flavour

ming

1 47

4

5

8

trient medium for the validation of aseptic filling lines for beverages

d for the validation of filling line components within challenge-testing

3< pH


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DHLERGROUP

Riedstrasse 7-964295 DarmstadtGermanyPhone +49 6151 306-0Fax +49 6151 306-278

www.doehler.comdmd@doehler com

ANALYTICAL SERVICES

Our accredited Analytical Services laboratory supports you with

analyses of your ready-to-drink beverages

- chemical/physical analysis (Brix, total acid, carbonation, pH)

- microbiological analysis (aerobic and anaerobic beverage-spoiling

microorgansims)

Further analytical laboratories offer you very specific testing, such as

flavour and sugar analyses and the detection of specific pesticides.

Make use of our comprehensive experience and benchmarks based on

the large number of beverage samples which we receive from the

international beverage industry for analysis.

You will save on investment and resources in the establishment and

maintenance of your own laboratories, space and staff. You can also

have your own results confirmed periodically by our laboratory, giving

security and creating trust.

Testing procedures, such as specific and non-specific cultivation, are

accredited compliant to DIN EN ISO/IEC 17025:2005 by Deutsche

Akkreditierungsstelle Chemie GmbH in representation of the German

Accreditation Council (DAkkS) for the subject areas of chemistry and

biology.

TECHNICAL SERVICES

Take advantage of the many years of experience of our technical staff

and save yourself time and money when completing projects at your

own company.

The DhlerGroups technical customer services / global technical

support are an essential part of the integrated solution from the raw

fruit ingredient to the ready-to-drink beverage.

Our technical customer services support you on site in

- sample fillings

- initial fillings

- recipe modifications

- mixing products

With its high expertise, our technical customer services also performs

analyses in microbiology and for hygiene management.

This provides you with an independent evaluation of the hygiene status

of your production equipment and filling lines.

qualityfoodsafetyproductfolderen_11-2012

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