r apid r eversed p hase u ltra -p erformance l iquid c hromatography a nalysis of the m ajor c ocoa...

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RAPID REVERSED PHASE ULTRA-PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS OF THE MAJOR COCOA POLYPHENOLS AND INTER-RELATIONSHIPS OF THEIR CONCENTRATIONS IN CHOCOLATE 學學學學學：學學學學： 09/12/10

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Page 1: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

RAPID REVERSED PHASEULTRA-PERFORMANCE LIQUIDCHROMATOGRAPHY ANALYSIS

OF THE MAJOR COCOA POLYPHENOLS AND INTER-RELATIONSHIPS OF THEIR

CONCENTRATIONS IN CHOCOLATE

學生：林瑋婷報告日期： 09/12/10

Page 2: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

IntroductionChocolatepolyphenols

ExperimentExtraction ProcessUPLC Analysis

Results and discussionConclusions

OUTLINE

Page 3: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

INTRODUCTION

ChocolatePolyphenols

Catechin Epicatechin B2 (epicatechin-4 β -8-epicatechin) B5 (epicatechin-4β-6-epicatechin) C1 (epicatechin-4β-8-epicatechin-4β-8-epicatechin) tetramerD (epicatechin-4β-8-epicatechin-4β-8-

epicatechin-4β-8-epicatechin)

Page 4: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

(+)-Catechin

(−)-Catechin

(−)-Epicatechin

Procyanidin B2

Procyanidin B5

http://upload.wikimedia.org/wikipedia/commons/a/af/Procyanidin_B2.svg

http://upload.wikimedia.org/wikipedia/commons/a/af/Procyanidin_B2.svg

http://upload.wikimedia.org/wikipedia/commons/a/ac/Procyanidin_B5.svg

Page 5: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

EXPERIMENT

Page 6: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

EXPERIMENT

Page 7: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

RESULTS

Page 8: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

RESULTS

Page 9: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

RESULTS

Page 10: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

RESULTS

Page 11: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

RESULTS

Page 12: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

RESULTS

Page 13: R APID R EVERSED P HASE U LTRA -P ERFORMANCE L IQUID C HROMATOGRAPHY A NALYSIS OF THE M AJOR C OCOA P OLYPHENOLS AND I NTER - RELATIONSHIPS OF T HEIR C

DISCUSSION

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