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TRANSCRIPT
The Impact of Diets supplemented with Medium Chain Fatty Acids on the Performance of Broilers Challenged with Clostridium perfringensIr. Manu De Laet – Product Manager Poultry @ Nuscience
Necrotic Enteritis (NE):
- Necrotic enteritis is an acute enterotoxemia
- Causative agent is a gram-positive, anaerobic bacteria: Clostridium perfringens
- Birds predisposed to NE when high levels of animal byproducts, wheat, barley, oats, or rye in diet
- Clinical illness: a severe depression followed by a sudden increase in flock mortality
- NE usually persists in a flock for 5 – 10 days, and mortality is 2% – 50%
1. Introduction
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1. Introduction
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Necrotic Enteritis Control:• Traditionally been accomplished by adding antibiotics in the feed:
– Virginiamycin (20 g/ton feed)– Bacitracin (50 g/ton feed)– Lincomycin (2 g/ton feed)– Ionophore-class anticoccidials
• Treatment for NE is most commonly administered in the drinking water
• Antibiotic free feeds ⇒ result in increased use of coccidiosis vaccines ⇒ resulting in early circulation of mixed Eimeria infections ⇒ increases the incidence of NE
Slide 4Source: Review on antimicrobial resistance
Deaths attributable to antimicrobial resistance every year by 2050
1. Introduction
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1. Introduction
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1. Introduction
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1. Introduction
• 1200 Cobb 500 males 0-42 days
• Randomized block design• 10 replicates / treatment• 30 birds / pen
• Treatments:• Negative control• Positive control (55 ppm BMD in starter/grower, 22 ppm Stafac in finisher)
• Aromabiotic® FULL (0.18 – 0.14 – 0.09 %)
• Aromabiotic® HALF (0.09 – 0.07 – 0.05 %)
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2. Trial
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2. Trial
Medium Chain Fatty Acids (MCFA) are :• saturated (=no double bonds or ring structures) , • unbranched (=linear molecule),• monocarboxylic acids (= only 1 carboxylic group –COOH),• with a chain length of either 6, 8, 10 or 12 carbon atoms.
Trivial name Systemic name Structural Formula
Caproic acid Hexanoic acid C6:0
Caprylic acid Octanoic acid C8:0
Capric acid Decanoic acid C10:0
Lauric acid Dodecanoic acid C12:0
Bacteria Host level
Highly Antimicrobial
Animal
Reducing pathogen virulence
Improving intestinal morphology
Improving immunity
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Thompson & Hinton, 1997 Santoma et al, 2006
Van Immerseel et al, 2004Boyen, 2008
Trial at ILVO, 2010
Piepers & De Vliegher, 2012
2. Trial
NE-challenge model • Day 0: Coccivac-B vaccination (live vaccine)
• Day 7: exposure to mild challenge litter
• Day 17: oral inoculation with Clostridium perfringens
• Day 21: lesions scoring (0 - 3) + weighing
• Day 42: weighing
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2. Trial
Negative control
Positive control
Aromabiotic® FULL
Aromabiotic® HALF
Body weight (g) 568b 622a 640a 614a
FCR (mortality adjusted) 1.38a 1.34b 1.34b 1.34b
Mortality (%) 1.36a 1.36a 1.00a 1.33a
Lesion score (score 0-1-2-3) 2.00a 1.23b 1.27b 1.67ab
Results 21 days
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a,b numbers on one row with different superscripts are significantly different (P < 0.05)
3. Results
Negative control
Positive control
Aromabiotic® FULL
Aromabiotic® HALF
Body weight (g) 1964b 2130a 2111a 2064a
FCR (mortality adjusted) 2.07b 1.97a 1.98a 2.01ab
Mortality (%) 9.01a 3.79b 2.62b 3.72b
EPEF 197.9c 245.7a 245.6a 234.7a
Results 42 days
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a,b numbers on one row with different superscripts are significantly different (P < 0.05)
3. Results
Conclusions
• The NE protocol used was highly succesful: Clear induction of lesions in all groups Poor performance High mortality in negative control group
• Aromabiotic® FULL was ás succesfull as the positive control (with antibiotics) in alleviating the negative effects of NE.
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3. Conclusions
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Thank you for your attention!Questions?