single cell assay on cd-like lab chip using centrifugal single cell trap microsystem research...
Post on 22-Dec-2015
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Single cell assay on CD-like lab chip using centrifugal
single cell trap
Microsystem Research Center, Korea Institute of Science and Technology
報告人:微機電所陳治忠
Types of signaling
Conventional method to isolate single cell
Chip design consisting of chamber channel and valve, and trap
Experimental setup
Control module CCD Motor parts of for rotating chip
Microfluidic component on CD-chip
Photo image of microfluidic pattern Schematic sideview of CD-chip
Cell trap assay
Cell trap VS duration time Cell trap VS trap size
Survival rate of cells with the treatment of PFA
Single cell assay at CD-chip MTT assay of crowed cells at 96 well plates
Rate of apoptotic cells with the expose of UV
Fluorescence microscope image of dead cellsFluorescence intensity data was cutoff at 10% of average value
Conclusion
• The ratio of trapping cells were almost 80% and more than 600 cells can be test in a experiment
• The trapping efficiency was main effected by the size of trapping hole
• Cytotoxicity assay showed the single cell might not be affected by the neighboring cells
• Fluorescein isothiocyanate (FITC)