studies on the gastric juice protein part ii...

TitleSTUDIES ON THE GASTRIC JUICE PROTEIN PART IIPAPER ELECTROPHORESIS-POLAROGRAPHIC STUDYOF GARTRIC JUICE PROTEIN

Author(s) KAKEI, MAMORU

Citation 日本外科宝函 (1959), 28(7): 2621-2642

Issue Date 1959-08-01

URL http://hdl.handle.net/2433/206972

Right

Type Departmental Bulletin Paper

Textversion publisher

Kyoto University

STUDIES ON THE GASTRIC JUICE PROTEIN

PART II PAPER ELECTROPHORESIS-POLAROGRAPHIC

STUDY OF GARTRIC JUICE PROTEIN

¥)y

MAMORU KAKE!

From the 2nd Surgical Division, Kyoto University Medical School

(Director: Prof. Dr. YAsU.¥JAsA Ao¥Acn)

and the 1st Clinic of the Internal Medicin, Kyoto University Medic旦lSchool (Director : Prof. Dr. Grnrcm 羽TAKIBAKA)

(Received for publication July 18, 1959)

INTRODUCTION

2621

The complexity of the protein component of gastric juice has been recognized

only recently. The most convincing evidence of its complexity was afforded b~’ the

study of the electrophoretic patterns and fractionation of the juice collected from several dogs by GROSSBERG, KoMARov and SHAY.1> Since then electrophoretic studies

of gastric juice have been reported by many investigators.2J寸JA charcteristic chan-ge in peptide and protein contents of gastric juice with gastric cancer, using polarographic and fractional precipitation methods was noted in a previous report.1〔）

This paper describes my further e百ortsto clarify the origin, nature and clinical

significance of the gastric peptide and protein, appl:yァinga combined new method

of paper electrophoresis and polarography.

I. FUNDAMENTALS

MATERIALS

A) 10 acid gastric juice specimens obtained from 10 normal cases.

B) 10 anacid gastric juice specimens obtained from 10 cases ＼＼北hachlorhyclr旬without gastric cancer.

C) 10 dialysed gastric juice specimens prepared as follows; 10 acid gastric

juice (material A) were dial,¥'Sed through a cellophane membrane against frequently exchanged cold distilled water in a refrigerator for 24 hours.

D) 10 dialysates of the acid gastric juice (material A).

E) Methanol filtrate prepared from acid gastric juice (material A) as described previously .10J

F) Blood serum of a healthy person.

G) Pepsin digest€d serum obtained as follows :

Serum was adjusted to pH 2.0 with 0.1-N HCl solution, mixed with crystal pepsin at 10-3 concentration and incubated at 37°C for 15 minutes, centrifuged for 15

minutes at 3000 rpm.

H) Pepsin digested anacid juice prepared as described above.

2622 日本外科宝函第28会第7号

I) B-bile aspirated through a duodenal tube from a healthγperson.

J) Gastric juice collc~tc:l frοm 2 Pavlov’S 1nuch dogs two and four weeks

after operation. Onl~＇ juices not contaminated with bile, blood or food were used, the contamin-

ation with saliva was minimized by strict precautions taken during collection of the

juice.

METHODS

The samples of gastric juice m℃1・G su¥Jscquentl}ァ白lkr仁d through a filter paper

(T,,::o Roshi :¥o. 5c). The materials other thanに rumwc1℃ neutralized with IN-

.:¥aOH anrl freeze-dried b~· b可〕hilization.

Electrophoresis : Lγophilized materials were dissolved at 5 % conceritration in a

！日ratebuffer at pH 9.0 and ionic str2ngth of 0.12 (prepared b:: dissolving 15.3 g

Na,B10i and 1.24 g H1B01 in lOOOml of distilled water as described by GLAss)11'.

Each 0.05 ml of this solution containing 2.5 mg of d1">' material or 0.05 ml of

serum were a11pliぷlto the center of each of the Toyo Roshi No. 51 paper strips,

3 cm widふ mountedin one cell of Toyo Roshi Co., and electrophoresis was carried

out against the same borate bu百er at 0.45 ma. per cm and 5 volts per cm, for 8

hours at room temperature. Imrnecliatcl~· after electrophoresis five strips were

removed from the cell, and 3 out of 5 strips were oven-dried for 30 minutes at

100~仁 Tnc first strip was stained with brom phenol blue stain12> (abbreviated as

BPB stain hereafter), the second stri1】 withperiodic Shiff stain12> (PAS stain), the

third日tripwas sprayecl with 0.2/r ninh~·drine in butanol saturated with acetate

bu町erof pH 5.0 after spraying with the same acetate buffer and heated to 90ごcfor 15 minutes.13＇人 11stained strips ＂℃ I℃ then scanned and traced in a densitometer

(Toyo Roshi C仏）, using a 2 mm slit.

Elution : The remaining乙strips wer℃ not dried or stain, but ＼＼℃1℃ cut into

16叩広mcnts, 10 mm wide, from 8 cm on the cathodic side of the application

p.>int to S cm on the anodic side. numbered from CS to AS, 1℃メpectiveb・.2 segments

con＂＜.：.~］ LJJld ing to each numl >er ＼γじl℃ put together into one jar of the same number目

2 ml of NaCl solution 01・2ml of 10 % sulfosalic~·lic acid was added to each of the

16 jars. These wじreplaced in a refrigerator for 12 hours.

Polarograph : 14nsns 16 electrol~·sis cells n℃l℃ numbered from CS to AS, cleansed

and dried thoroughly. Into each cell n’as poured 1.0 ml of trivalent cobalt test solution which was prepared as follow品：

人） 2-N ammonium chlorick' solution

B) 2×HJ 'N l1cxaminic cobaltic chloride (lutcosali) 飢えution C) 2-N ammonium hyclrυxiclc solution

The test solution ＼γas prepared Iηv adding ju日tbefore the cxperimcnts in this

order; 1 vol. of i ¥. 1 vol. of H, S ml. of C. To each c仁llof the same number was

aclclcd 1.0 ml of each υ1・tlie16 eluate日. PolarogTam'> wcr心 taken immediately in

each cc:ll und.::r the same conditions 出 prじγiously (L'icribccl. ScnsibilitY of the

galvanometer was 1/50. The series of polarograms from CS to人8,thus obtained,

STUDIES ON THE GASTRIC JUICE PROTEIN 2623

was called a paper electrophoresi~←polarogram (abbreviated as PPgram).

RESULTS

1) Comparison between acid and anacid gastric juice Materials r¥ and B were used in this stud~·. The average imper electrophoretic

pattern of acid gastric juice as compared to that of anacid gastric juice is shown in Fig. 1 (a and b). ¥Vith the BPB ぉtai11,5 main peaks are found in the electro-phoregram of the acid gastric juice. The~· are named P1 to Pり countingfrom the

Fig・. I Comparison of electrophoregram of acid and an畠cid広astricjuice before and after dialysis, dialysate, and methanol白ltrate.

BPB

PAS Ninhydrine

a) acid gastric juice c) after dialysis

，， nr 、、、，、

’、ー’－．／・ー『・ヲ＂－・，，－・、・~・ー・、＼‘，一司．ー、

2101234 ,_,

b) anacidεastriじ juice d) dialysate

6 ｛＋？ 6

e) methanol白ltrate

／＼ r ' ' ＇’ l ” l

6 54321012345 1－令，1-1

most anodic peak to the most cathodic peak. On the other hand, in the electrophore-gram of anacid gastric juice 3 (P_・, PJ, P.1) out of 5 peaks are prominent, and the remaining 2 peaks, P1 and Po are vc1・＞.low or absent. With the PAS stain only one difinite peak is found in the elcctrophoregrams of both acid and anacid gastric juice. The peak of the anacid juice is more prominent but its distribution is more narrowed on the anodic side than that of acid juice. With the ninhydrine stain, the peaks are more abundant and prominent in the electrophoregram of the anacicl juice than that of the acid gastric juice.

The PPgrams of acid and anacicl gastric juice a1℃ shown in Fig. 2 (a and b).

第 7号

charaterized by vcr~· low, or absent protein waves

in other wurcls, their distribution is narrowed on

第28巻日本外科宝函

The PPgram of anacid juice is

in segments人～ A,,(',and （‘＂

2624

Comp:nison of PPgram of acid and anacid gastric juice before and after dialysis, dialysate, and methanol filtlate.

Fig・. 2

ρw pL

nu

－－

f

I

I

t

eo a

σh ju

－－

nL a

a

d w

．、、、、、‘

， Z

ωH 甘レIof

／／／／

)

《lI dialysate.

(:5

A6 As A今C今C5


e) methanol filtrate.

C6 C~ C今

both the anodic and cathodic sides and by the t~·pical double wave with the low

minimum point on both sides. The paper electrophoregram stained ＂社 hBPB stain

and the pattern of PPgram are nearly the same (as shown in Fig. 1). However,

in the PPgram of the acid gastric juice protein waves have a wide range, from

segment A.1 to℃， with a single wave shape and markedly inhibited cobalt maximum

on the cathodic side, and the t~マpical de〕ublewave shape on the anodic side. Espec’

ially in S色gment

shapes of other segments.

2) Influence of dialysis on electrophoregram and PPgram of acid gastric juice

Materials A, C and E were used in this study. The averaged paper electro-

phoregrams of acid gastric juice after dialysis are shown in Fig. 1 (c). With

regard to the paper electrophoregram stained with BPB stain, all peaks are increased

in sharpness but the height of P5 and P』 aredecreased by dial:yァsis. In the electro-

phoregram stained with PAS stain dial~·sis has little influence on the peak. With

regard to the electrophoregram stained with ninhydrine stain peaks are markedl~·

diminished or disappear after dialysis. The electrophoregrams of dialysate and

methanol filtrate are shown in Fig. 1 (d and e）.人fter dialysis the protein wave

of segments A4 and A5 are slightly increased in height, but those of Ci, CJ and C"

are decreased or have disappeared, and show a more prominent cobalt miximum at

Ca, C4 and C1・ The PPgrams of the dialy泊 te and methanol filtrate are similar

(Fig. 2 d and e). Protein waves are present from C to A2 as single waves. This

single wave is named the peptide wave.

,_,

3) Protein precipitable by sulfosalic:yァlicacid in PPgram

Fig・. 3 Comparison of two PPgram obtained from 0.95% NaCl eluate and 10% sulfosalicylic acid eluate.

a) acid gastric juice.

ー一一・ patternof PPg・ramobtained from 0.95% NaCl eluate. ・一一 patternof PPgram obtained from 10% su日osalicylic

acid eluate.

,_,.,

2626 日本外科宝函第28巻第7号

b 1 anaci<l gastric juice.

，＿》 fす｝

c) tur‘bidity of electrophoretic fraction with sulfosalicylic acid added (anacid juice;,

5ιz十，

The pattern of PPgrams obtained from 10% sulfosalicylic acid and 0.95% NaCl

eluates of acid ancl anacid juice are shown in Fig. 3. In acid gastric juice these

two patterns a1℃ similar, but in anacid gastric juice the former is lower in segments

Ai, A,, A 1, and C¥ than the latter. The turbidity of 0.95% NaCl eluates with

Fig" 4 Changes in PPgram after pepsin digestion of anacid gastric juice and serum.

C6 Cs

""' /{(" 八、八今

/ al anaιid gastric juice.

〆•

b 1 digest~d juice.


じJserum.

が〆〆C6. Cs C今 C3 C2 C1 I A1 A2

<-•f---0 ー→＜＋l

,r 、

d) ・digested serum.

〆〆〆〆 F /I" I C6 Cs C今 C3 C2 C1 I A, A1 A3

tー｝←－6」斗r+J

Fig. 5

a〕 caninegastric juice collected 2 weeks after operation of pouch.

CG

bl canine gastric juice collecteJ

4 weeks after operation of pouch.

2'328 日本外科宝函第28巻第7号

Fig・. 6 PPgram of bile.

10拓 sulfosalicylicacid was scanned, traced and shown in Fig. 3 (c). Segment

A3 is the most turbid. 4) Influence of HCl-pepsin digestion on PPgram of anacid gastric juice and

serum Materials F, G and H were used in this study. The PPgram of the anacid

juice specimen, serum, the pepsin digested anacid juice specimen and serum are

shown in Fig. 4. In the PPgrarn of the digested gastric specimen the protein M引℃日 occurin a wide range from A0 to C,, as a single wave form on the cathodic

side and as a double wave on the anodic side. In other words, PPgram of the

pepsin digested anacid gastric specimen is similar to that of the acid juice specimen.

In the PPgram of native serum protein waves occur from A3 to C.1 and have

the typical double wave. The protein wave in segment A" and Ai are the most

prominent and characteristic, corresponding to albumin, and di汀erentfrom thヒwavecorresponding to globulin.

In the PPgram of digested serum, protein waves have a wide range from A,

to c, as t~·pical single w引でs,or anomalous waves with a high minimum point.

5) In日uenceof operation on PPgrams of canine gastric juice

Material J was used. The PPgrams of gastric juice collected from pouch dogs

2 and 4 weeks after operation are shown in Fig. 5. In the PPgram taken 2 weeks

after operation the peptide ＼＼’aves appear as far on the cathodic side as segment

CJ・ Inthe PPgram 4 weeks after operation the protein waves are found in an almost normal range.

6) PPgram of bile

This is shown in Fig. 6. The protein waves are found from i¥; to C2 as the

characteristic double wave with marked！~· inhibited cobalt maximum.

DISぐUSSION

Elcctrophoretic studies on dial~’日cd gastric juice protein have been reported I〕）＇

ma町Y investigators, but there have been few on nondialysed gastric juice protein.

In the previous report10J it was demonstrated that a detectable amount of

peptide wa日 consistentlypresent in acid gastric juice, and this peptide was soluble

in methanol and was dial；γsable through a cellophane membrane. The p~tide in


the methanol filtrate and dialysate have the same cathodic electrophoretic mobility in a borate bu在日・ ofpH 9.0 and the same polarographic single wave as shown in

Fig. 1 and 2. It seemed that both peptides are almost identical.

The,di百erencebetween the PPgram with and without dialysis as shown in Fig.

2 also indicates that a considerable amount of dialysable peptide is present in acid gastric juice.

The protein of anacid juice is eletrophoretically in narrow range both on the cathodic and anodic side from A3 . to C 1, and polarographically characterized b~· a

typical double wave. However, the protein of acid juice is electrophoretically in a

wide range from AョtoC1, and pol.arographically characterized on the cathodic side

by a single wave, which is similar to the wave of pepsin digested serum, and by

at~’pical double wave on the anodic‘side. Particularl~’ the protein wave in segment

Ai shows a charateristic double wave with a very low minimum point. Because this segment A1 corresponds to segment M1 of GLAss,17i this protein wave is prob-

ably produced b：－’ the mucoprotein of GLAss. This protein wave is named the mucoprotein wave. The mucoprot~in wave is more prominent and typical in the

PPgram of acid juice after dialysis, and is not found in the PPgram of anacid juice. When anacid gastric juice is di乞ested bJ・ HCI pepsin, the mucoprotein-like

wave is found in the same segment A 1・ Fromthese data the possibilitJ’ cannot be

excluded that mucoprotein is also the pepsin digestive product.

It is evident that the protein of anacid gastric juice has an electrophoretically

and polarographically di百erentnature from the protein of acid gastric juice. It may be emphasized that the anacid pattern is changed to the acid pattern by pepsin

digestion just as the native serum pattern is changed to the peptide pattern. GLAss isi has reported that the electrophoregram of the gastric jucie of patients with

gastric atrophy is characterized by the complete absence of pepsin, M1, Y and Z

peaks and an absence of, or a very low, X peak. These characteristics of gastric atrophy are always found as the pattern of anacid juice. It is more reasonable for

the characteristic pattern of gastric juice of patients with gastric atrophy to be called

anacid pat七emnot atrophic pattern, although achlorhydria and gastric atrophy are

intimately related. In other words, the gastric juice must always be anacid for the atrophic pattern 句 appear.

In anacid gastric juice protein precipitable by sulfosalicylic acid is consistently

present as described previously.10i This protein is electrophoretically distributed from

A3 to C1, especially in segment A3, like serum protein.

GLAssm reported that soluble mucus was present in filtered gastric juice and

corresponded to the middle peaks of the electrophoretic pattern of whole gastric juice, and was precipitated with trichloracetic acid. The protein precipitable by sulfosal-

icylic acid may be identical with GLASS’s soluble mucus. It is interesting that HC、ト

pepsin digestion causes this protein in anacid juice to disappear, w

mucoprotein-like protein appear.

With regard to dialysed acid gastric juice, the protein waves in segments c~ ，

C3, C4 and Cs presumably correspond to the X, Y, Z components of GLAss.11i He


reported that the X component contained almost no carbohydrate, only a small

amount of protein and much nondialysaiJle material unidentified 邸内t. The protein

waves in日cgmentsC'i and c, are much lower than would appear from the densito・

metric tracing stained with BPB stain. These data indicate that the protein waves

in segments C" and C, correspond to the X component of GLAss. The Y, and z components are the most positivelyア charged cathodic components, and contain no

protein or carbohydrates at all according to GLAss. The protein waves in segments C,, Cr. are vc1・:_-low or absent and are characterized IJy the inhibited cobalt maxim-

um, although height of the cobalt maximum is increased to some extent after

dial.mis. This indicates that at segment Co, C5 cobalt maximum inhibiting high

molecular substances are present. In the PPgram of bile the cobalt maximum of

the protein wave is markedl~· inhibited, especially on the anodic side. Inhidition

of the cobalt maximum is closely related to surface active agents in general. The

substance inhibiting the cobalt maximum in bile is presumably bile pigment, because bile pigment has anodic clectrophoretic mobility. In the PPgram of gastric juice

obtained from pouch dogs 2 weeks after operation the protein waves appear widely

on the cathodic side as far as segment G;. It seems likely that an increased amount

of protein, which is sec1℃tcd from the mucosa of the post-operative gasfritis, is digested

h~· pepsin and broken clown to peptide. BALLE-HELAERs20' and SAsAi''' have reported

that the first maximum of a protein double ＇＇’ave originates in protein-bound poly-saccharides in general. Indeed, in the area showing the typical double wave polysac-

charide is al ＇＇ァa~·s present in the PAS stain. On the contrary, in areas showing a

single wave polysaccharide can not be found by PAS stain. Therefore the peptide in

methanol filtrate or dial｝判tte may not be conjugated with polysaccharide. The single wave with high minimum point is also found in serum albumin22'23' and ovoalbumin初 25'denatured bv alkali or heat.

The characteristics of wa＇℃ sha1ic rn町’ bestudied as an important approach to the problem of the structure of protein molecules.

II. CLINICAL OBSERVATION

On the basis of the experimental results described above, clincal studies were

carried out with special emphasis on cancer of the stomach.

l¥L¥. TERIALS

Studies were carried out on 59 subjects with the following diseases : Gastric cancer 19, precancerous state 2, peptic ulcer 7, duodenal ulcer 8, aplastic anemia 3,

other diseases with acid gastric juice 13 (gastric ptosis, chronic gastritis, cholelith-

iasis), gastric polyp 1, leiomyoma of the stomach 1, other diseases with anacid

gastric juice, 5. 人11cases with the exception of 3 with aplastic anemia were

operated upon, and the白nal diagnosis was confirmed in all instances ]),・ histologic

exa.mination of the resectecl specimen in the Pathological Laboratory of the KJ・oto Umver日ityHospital.

METHODS

1） λspiration of gastric juice


After fasting for about 12 hours, gastric juice was aspirated through a REHFuss stomach tube, followed b~’ caffeine stimulation (KATcH-KALK method), or sometimes by histamine stimulation. During the aspiration of the specimens, the greatest possible care was taken to avoid contaminations with saliva, blood or bile. Specimens apparently contaminated with bile, blood or food were not used. The specimens were subsequently filtered through filter paper (Toyo Roshi No. 5c).

2) The free acidity was titrated by using as indicator ToPFER’s reagent. 3) Polarograms were taken on 3 fractions: buffered fraction (Fb), sulfosalicy-

lic filtrate fraction (Fs) methanol filtrate fraction (Fm) as described previously.10) 4) Paper electrophoregrams of BPB stain, PAS stain and ninhydrine stain,

and PPgrams were made on each of the nondialysed lyophilized gastric juice spec-imens as described above.

5) Paper electrophoregrams of BPB stain were divided into 5 areas correspon-ding to the 5 main peaks. These areas were termed B1, B", BJ, B4 and Bo, counting from十5cm to十3cm,from+ 3cm旬十lcm, from十lcmto-lcm, from-lcm to-3cm, and from -3cm to -5cm, respectively, and each area was measured by a planimeter. With regard to the PPgram the wave height of each area was calcul-ated as follows: the wave height of Bi, Bi, BJ, B4 and B5 was repesented by the wave height of segment A4, averaged wave height of two segments A3 and Ai, A1 and C1, C, and CJ, and the wave height of sement C1, respectively.

Table 1

e

m

a

N

O

N

一

｜ tイEle仁空o~h~~主f士Free acidit刊－ I I I I Value Value Value ¥ B1 B2 ~ I B4 ¥ Bs B1 B2 B3 I B4 Bs ピ

274monOTA4よbqdnヨ一nu

qd4つん

A‘ηLの4。，uηL？－ヮ“

一

qJ

tAduzに

dηJUFhJ91utAウd内

LnU

一4H宮

oonvnutiFDのJunuoonudcd

一pb

1ム41。，U1A1AtA1A叩i

？

A一1A

3354010952

一5

qu司

υaqqus公司

υ司uquqJqJ

－qd

7943769709

一1

つ＆つ＆ウ

bquつιuqdquηLqdつJv－

QU

1560330984

一5

TA－－っ－

TAη4nL

’AtA－

1A

4709040928

－9

2123212Il

－－

no。ο守’nvにdマ’4・TLOOPD

－pb

。ι。ι。J－ηノMのAMη’U1Aqd1・・つ＆

一のふ

9924960176

一6

nLつん勺

δηLtAの，Lη。。，

U内Jhqム一。，“

円

tのι00ワ

tQuqJ4－pb’iηL－’

A

1AnL’且つ＆

1A1Aのム

1Aquっ’u一つ

4

2931702429

一9

’A唱

A司Lti

p

0

0

0

一

ono泊

DoooonA

pnDamp－ppap－Pη

一

yαywuyyyyym一

hnhnhhhhhb一

r同

一

e－

一

m

一lm

州

一

t

－k

．1a

似

a

一e

－為・

IAMhimu

g

－u

問

自

問

’

ka．官

、

hataa

一，u

r

－kahd

凶

bkn

－口

北－

S沼

aa－－

ιaau一口、

日

日

－

mkkaEtn比b一

m－r】

aivaaaε

一z

g

一ugTHhHHTMK↑

鋭、

d

－

－r

－

e

ρし

w

－

－

aE3130733690－町

司

tqdnv’inLa告に

JW店VGV弓

r

一

qruqム今、υ勺ο内

J勺O勺οηJqdqru一

anacid gastric cancer 。。内ノ嗣εu

qι

nU4uEEinU4笹田ヨマ，tA7too

qυA’q’－A噌つ’M1Aマi？uE且？“

ハU弓’44EFDAせ00口daιτtihud3424211222

247 Usui anacid 18 7 7 284 Baba anac】d 0 15 40 40 5 ' 0 40 35 16 9 29 15 17 292 Nishi an acid 40 22 23 306 Tani no anacid 5 31 30 31 3 IO 32 33 24 i I 37 20 20 324 Mori anacid 6 30 22 36 6 9 55 23 13 0 23 6 ll 333 Ueyanagi anacid 2 34 31 30 3 6 35 40 118 I 15 5 ヨ348 Yamagata anacid 111 32 28 27 2 10 40 30 18 2 20 8 9 350 Akamatsu anacid 5 31 47 17 0 0 37 4.J 19 0 22 12 IO 354 Kinoshi』ta anacid 6 15 I 28 32 19 I I 0 38 35 I 16 1 I 11 4 i

average value 1

1

4

つ－！

っbー

マt－

co qυ

－ nud qu

pnV

1

4

“．

「

11111

守

4。，fats

l

にdqο QJ 。ι戸、JV 12

2632

precancerous states

日本外科宝函第28巻第7号

338 1 Mise 361 Kawasima

average value

peptic ulcer

hypo hypo I !ll! I ~； i ~~ I ii I ：~ )Jtl叫！引！？し！？

304 Oka 311 1 Nakazima 323 I Ukita 325 I Murata 339 I Odati 352 I Kimura 362 I Yokota

average value

duodenal ulcer

hyper nor mo hyper hyper hyper

1 hyper hypo

quRυqtUFORdAUハUnmu

－－

ηLtA唱

AtAqぺuti－

唱

A

5

5

4

8

3

5

2

一5

8

3

0

7

1

4

1

一FO

－－‘ltinLつbtA唱

A一

Tよ

2

7

1

0

3

6

0

1

川

qυnJAAQυqdnJA時

A

n

J

8

5

6

0

1

6

3

一7

nLqυのム

η4つムつム

ηJ一つ’U

7

0

9

5

2

9

4

一7

1ATよTA1Aつ＆

TATA

－－A

弓

tnutAつhFbAせQd一

戸

b

つ白tAのん

411A

一1よ

A吐勾

400ワ’

η3nuoO一

Fb

のん

η41AnLつ＆

qυつん

ηJU

FU4‘qJ

’iワtFDA－にd

ηペUηLnノしvn〆』

ηノun，iMηム一

η／“

nUQU弓

dFDQdnUA宮

nu

η乙1ムのん

11司

1ηLのん一つ－

Aq

’I1iFhdRυ唱

IRd

一Ru

ti－－

n4141i11

喝

a

i

t－

301 I Okuwa 309 I Matsutani 328 I Yamasaki 330 I Minami 336 I Fukui 340 I Yasui 363 I Yamamoto 371 I Ebara

average value

hyper hypo hyper hyper hyper h:yアperhyper hyper

つ白内LFhvRunua生ヴdno

一

η41ATATAqu1i1A

一

4nbpbっd1AqunLnL

一一qυ

円uqu円

tヮ“

nuA－nL氏U

一PU

z－

－

2甲

AI1

－41

14ququpbqυQνA＆

QU

一J

3

3

3

3

3

3

3

2

一J

9

1

3

6

1

0

4

0

一9

2

3

2

2

3

3

3

3

一会

6

1

2

4

5

4

8

3

一9

叩

AのJunJJUのム

ti

’Atiηι

一

11

qu只unuワuウ

d弓

d’IQd一

FU

4OAT－ηL1ATA1i14

一74

9

9

2

3

7

0

5

9

↑

4

‘1つんのLつん句AのLつ’uqu一

ηι

0

2

6

2

7

3

1

5

一7

3

2

2

2

2

3

3

2

一宮

4AnuaunLauQdQUAせ－

qu

つム

titiηL11111A噌

i一

唱

A

d生っ－

4t1よ

Tよ胃

iえdqυ

戸、υ

匂

1ムTAT－

ηん内ノ釦

11111lTよ

aplastic anemia

泌5 1 Miy?si ! hyper 16 I 20 30 I 24 I 10 20 17 1 30 I 25 i 8 18 337 I ma1 nor mu 20 I 19 22 I 24 I 15 24 22 ' 30 ! 18 ' 16 13 35S '. Yamamoto j hyper 16 j 20 24 j 22 j 18 ! 16 30 ：必 l12 I 2 14

average value 171初 I25 1 23 ! 15 I 20 . 25 34 I 2s 13 ¥ 15

Others 1 acid 1

1 I 12 I 16

345 I Nakatani 346 I Funagoshi 351 I Nakae 365 I Suzumura 307 I Hatano 322 I Masuda 347 I :¥Tishig-ami 360 I Takarada 370 I Wakabayashi 232 I Asai 220 I Hiranabe 391 I Izmniyama 380 I Itり

average value

hyper hyper hyper hyper normo hyper hyper normo nor mo hyper normo nor mo nor mo

gastric polyp

266 j Ta叫 a j anacid

Leiomyoma of the stomach

270 I Hoki anacid

AA

ηLnunUF300にdQdQd－bFO7勺

δ

Q

U

5

14つ白内41ATA1ょ

Il

－－’i11‘1

1

i

且ゐつんつん

QdFUA官円

jnJ只UFbquqυqδFb

ハJnJndndqυquηJquqυ勺

3ηJnJnJ

匂

υ

3

4

9

1

7

0

6

2

5

9

8

1

8

0

qυヮ“

ηJbnJqLquつdquqυη4つ’uつdqL2JV

巧

tqdFbqδつムウ

4pbqοnUFOOOハUウd

氏υ

1A1ょ1ム1414司

A噌

in4141i11のム

1A

’A

4FbpU4nundヴ

4ηOFbRdn4Qd14

一qU

14吋

i1ょ1AηJhtiti

’i’i

唱

A

’i一

司

i

O

5

7

0

2

4

8

5

1

i

5

5

0

7

一6

ηLつJunLqυつんつん

n，ムワム

qυのムつLqdのム一ワム

8

2

8

9

3

6

3

6

5

6

7

7

4

一7

2

j

2

川1

J

1

2

1

1

1

2

7

一一2

2

1

9

7

8

2

4

0

8

9

0

1

1

一

D

つん

ηLT4Tよ14η，Lワムのム

TAT－η乙nLη4

一

qU

FO7anunU弓

tFbaU氏U1AFUPonU月

i

一A時

A

Il－

－

1

1

1

1

1

1

1

1

1

4斗ムヴdFbnUA品

IPOワtnUAせ弓

tTA氏uqd

－iのんつんつん

qJ14qυつム

1Aヴム

1ATA

QU

戸

b

勺’qLrooocOのんハU・4FOのム

dq

ーム

TATAηJU

のんのム

ti

’A唱A唱

i

qU7’巳

1uハUハUCO勾

tnuqJ7anuιvnD

1Aワムヲ“。4qu1ム

qυっ“

14つμT4Tよ

1 I 40 I 44 i 14 I 1 I 4-j 43 I 37 I 16 I 0 I 22 I 13 I 14

7 I 42 I 31 J 14 I o J 15 J 46 I 31 I s 1 o I 31 I

2633 STUDIES ON THE GASTRIC JUICE PROTEIN

nhMA坐poqddA20D

っυAaにdqJdιτ

勺，

CO

戸、u日vdAτQdpo

ー

ハUnununununu

5 I 39 I 42 J 13 I

nuqJnUTAAunu

anacid anacid anacid anacid anac!d an acid

Others (anacid)

I Murata [ Hosok i

IM包Kaimot 0

Kanda 止。

average value

329 356 367 358 373 380

5 4 9

non~cancer (acid) average

’，， 4 16 !

4 4

丹、

υ。。つLOO

－

S斗鼻l

p

ヘυつLPhv

2

nu

nL l

Fhυ ー

RESULTS

Detailed data of the polarograms of 3 fractions (Fb, Fm, Fs), the electropho圃

regram of BPB stain, and the PPgram of 59 cases are presented in Table 1. No.

380 is a patient with caffeine refractoryァ anacidjuice, but with acid juice following

histamine stimulation.

1) Comparison between gastric cancer

gastric juice

The average electrophoregrams and PPgrams of bothシgroupsare shown in Fig. 7.

group with acid group and non-cancer

Comparison between gastric cancer and non-cancer cases with acid gastric juice.

Fig・. 7

non-c且ncerb1 PPgram.

r-ー「

~ B, ~

a 1 BPBstain. tu cancer

ιーー－85

」；口；lf

ao

:2..0'

10

Bz

BPB stain: In Fig. 7 a the BPB staining area of Bi of the cancer group is

smaller than that of the non-cancer group. On the contrary, the area of B5 of the

cancer group is larger than that of the noncancer group. The remaining areas of

both groups are almost identical.

PPgram : In Fig. 7b there is vc1・~· little difference . between the two groups in

wave height (area), but a marked difference in wave form. The typical mucoprotein

wave (segment A1) is found in only 2 out of 10 cancer cases but is found in all

except 2 noncancer cases, being especially prominent in cases of duodenal ulcer. The

peptide wave (on cathodic side) of gastric cancer is charcterized by a high minimum

point against the wave height, and by markedly -inhibited cobalt maximum. The

63 84 82 島B+ 85


Fig. g Proportion of height of minimum point to protein wave

height at segmel!t C2 、.vaveheight

100

qo

~o

'ID

60

50

ω

30ι ：タ＇~J(20

10

． • non-cancer case.

X cancer case ．．． ')( )(

x

Prote tn wt.Yo otεegrue11t C2,

nl)n-e‘mcer. cancer.

~·暗号 of minlm~

0 Io 20 30 40 50 60 刊、 so 可九相

Fig. 9 Comparison of cancer and noncancer in height of cobalt maximum at Cs

100 ．．．．． ~o ．・．80 ．． 70 ． 60 －．．． 50 ．． ω ．・．．

－・30 ．． 20

．．．．・．．

10 ．．・3e.sfric non-

cancer canαr

Fig. IO Comparison between gastric cancer and non-cancer cases with anaci<l gastrc juice.

a> BPB bl PPgram 一一→【non-cancer

仁トでcancer

ltO

口一！日t

’’’’1 ．．

．．－－

p

・FE－－E・E・－EBEE－d

・・－－

－－

B~ B+ 83’ Bz 81 B!i B+ B3 B2’ B1

proportion of the height of the minimum point to the wave height in segment Ci

and a typical peptide wave of cancer and non-cancer cases are shown in Fig. 8.

The two groups are compared in terms of the height of the cobalt maximum at

segment Cs as shown in Fig. 9. Moreover, the cases characterized by a high min-

JO

2.0

10

r---,


Fig. 11 PPgrams of cancer and non-cancer

Cc. c~ c.. cち c12_1三~i」九、A2peptic ulcer

precancerous state

No. 338 (hypoacid〕

6

訓

1l

．

ぺ

i

J

A

ilv＼

内

十

11

ん

AH

WJfii

llrM

h

t

4

d

lF

F

F

f山V

－

1－／Lm

J

i

l

lL

／1

1f

叶

rlJム

－

d

l

1

0

A

／

11

／じ↑

4

1

I

I

P

E－

－

／

t

』

附

仰

JJJ＼

～

パ

刊

し

川

l

t

a

川

r卯

日

1jJpk

e

y

J〈

氾

h

阿

U

〆’t、

自

3

・v九

勺4

4

‘

punノ“

rh

’

叶

Mvk

ll〆Q

a

F

－u

C6 Cs

2635

2636

川4

(6

日本外科宝函

gastric cancer i¥o. 348 (anacid)

achlorhydria

II

/"'

Cら

ト＼0. 367 r anacid I

第28~会第7号

／ ,.

、，Ac

imum p >int indicate a high Fm value on fractional polarographic anal~吋日．

2) （‘1Jl11]l'.tric.;・i11 k't ＼＼で℃n!nth groups with anacid juice

The 2広rnup,;wc1じ compar~：d in terms of the average ckdrけplmrcgramof BPB stain and lhc PPgram as shown in Fig. 10. There is little cli町crencc between the

2 groups in regard to the electrnphoregram and PPgram which are characterized

b＞守 anarrow ran広eof

double waves. In a fじw caneじrca日じヘ anomalous wa ＼＇じベ礼ppじ礼1・at scgmen十日 A可 and

,¥ Thcsじ anomalouswa v品 di:-;apJk:aral"Lc:1・dial＞刊i日．

Typical PPgrams of cancer and non-cancer caメピメ a1℃ shown in Fig. 11.


gastric polyp !¥¥o. 266 (anacid;

C6 Cs C今 C3

C6 Cs Ct, C3 As Ab

／

／ノ

Fig. 12 Electrophor唱egramof PAS stain ，『h、，、、

3 2 。

十一・－cancerand non-cancer (anacid) -----cancer (acid juice) 一一一一non-cacncer(acid juice)

2 3 4- 5

3) Electrophoregram of PAS stain

2637

The two group3 are compared in terms of the average electrophoregram of PAS stain as shown in Fig. 12. In the anacid gastric juice there is scarcely any di百er-~nce between the two groups, but in acid gastric juice there is a di百erenceon the anodic side as shown in Fig. 12.

4) Electrophoregram of ninhydrine stain.

In the electrophoregrams of cancer cases the peaks are more numerous and prominent than in non-cancer cases as shown in Fig. 13. These peaks are dimin-ished or disappear after dialysis.

DISCUSSION

Although the electrophorctic pattern of gastric juice is variable as has been

2638 日本外科宝函：~~28、会第 7 号

Fig" 13 Electrophoregr旦m of ninhydrine stain. a) non-cancer cases.

q 8 '7 （ー？

b) cancer cases.

7 8 ｛十7

'l t十｝

reported b~· ma川・ im·c~tigators,' ＞→＞，23> 5 main comp_inents are generally found in

the author's data.

The component (B,) of the most anodic electrophretic mobilit~－， which corres-

ponds to the component l¥I, of GLAS!", 17> is incrca日eelin acicl nmトcancerjuice, especially

in duodenal ulcer, dccrca~引l in acid gastric cancer juice, and absent in all anacid

ca日cs. These data aどrec¥¥・ith the data of other ill¥℃討ii日＼tors.L3＞・si,9>This component

is p1〕larographicallyrepresented as the charactcriメtic protein wa ¥'C w hie h is named

the mucoprotcin wave. The mucoprotcin wave is t~·pical in acid non司 cancerjuice,

especially prominent in duodenal ulcer, low or at~·pical in acid gastric cancer

and not pr℃sent in achlo1・hyclria. It is interこsting that th2 ri叩 and fall of the

mucoprotein wa ＼℃ parallel日 thatof the glandular mucoprotein obtained l乃ア the

acetone precipitation method of GLAss.27> The mucop1叶仁i11 ＼＼川で ma~’ be related to

th;:; intrinsic !'acor of （句ASTLl,,Ji了causeglandular mucoprokin scn・cs a日 the intrinsic

factor according to GLAss.盟）入lthough NoRPOTHu ancl GRossEERG1> ha＼℃ 1℃ported

that thじ fastanodic moving component l℃pn::sent日 thesame cnz.' matic action as

pepsin, the mucoprotein wave evidently cli百en;from the prnt仁inwa＼℃ of pepsin itself.

The component (B,) of acicl gastric cancer stai11日 υ111.'・ slight!.'・ with PAS stain.

The rc・a-;1m for thi河 differencema）’ be that glandular muco1】roteinis decreased in acid cancer.

The component (B,) of the fastest cathodic electrophoretic mobility, which

STUDIES ON THE GASTRIC' JUICE PROTEIN 2639

includes components Y and Z of GLAss,11> is increased in acid gastric cancer, and

absent in achlorhydria, and docs not stain 1γith PAS stain. This component is

characterized b~， a low cobalt maximum in the PPgram. The protein ¥vave of

this component is much lower than would appear from the densitometric tracing

stained with BPB stain, especially in acid garstric cancer. The cobalt maximum is

markedly inhibited in acid gastric cancer. It indicates that there is a large amount

of cobalt maximum inhibiting factor and a low polarographic-active substance in the

gastric juice of patients with gastric cancer. The author has rcccnl;., obtained some

evidence that the toxohormrnひrcr淀川cmay l::e located in this ccmp;nrnt, but this is still under investigation.

The slow cathodic moving component polarographically represents a single wave

which is named the peptide wave in the acid cases, and a tγpical double wave in

the anacid cases. There is a difference between acid cancer and non-cancer in respect

to the form of the peptide wave. Gastric cancer is characterizcd by a peptide wave with a relativアelyhigh minimum point to wave height ratio as shown in Fig. 8. It is unexpected that there is no significant di汀erencebebveen cancer and non-cancer

group with regard to m’ave height of Rand B5・ Thecase characterized bγa protein

wave with a high minimum point in the PPgram represents a high protein value in

the methanol filtrate (Fm). This indicates that the1・cis a large amount of peptide

in acid gastric juice obtained from gastric cancer.

It is evident that the low or atJァpical mucoprotein wa＼℃ and the peptide wave with high minimum point and markedly inhibited cobalt maximum can be used as

factors in the diagnosis of gastric cancer, if the gastric juice is acid.

The distribution of proteins in the elctrophoretic pattern of anacicl gastric juice

appears similar when polarographγis applied to the eluates of segments cut from paper electrophoretic strip日. The clectropho1℃tic distribution of the proteins of anacid

gastric juice is in a narrow range both on the anodic and cathodic side. These data agree with the data of other investigators.≫,s> 9'-lB>,19> The protein waves when

present are al wa:-・s found a日 tγpical double waves in the PPgram. There is no

di町erencebetween anacid cancc1・ and non-cancer in respect to the electrophoregram

of BPB stain and PAS stain, and PPgram. In consequence the di首erencial diag-

nosis between anacid cancer and non-cancer is impossible. The incidence of atrophic

gastritis is too low to diseuss.

The slow anodic moving components (B" and BJ), ＂七ichpresumably correspond

to the mucoproteose of GLAss,11> are large in anacid cases. These data agree with the data of other invcstigators.3>·8＞.~＞， 18>·19> The protein waves of these components

are found as typical double wa vc日 inthe anacid cases, and as atypical double waves

with a high minimum l〕ointin the acid cases. Because the protein precipitable by

sulfosalicylic acid is distributed in the same area 出 described above, the typical

double wave appears in this area in anacicl cases.

It is evi【lentfrom the protein w

electrophoretic mobilih is not alwa；.・sthe same.

In the elcctroph01℃gram of the ninhydrine stain, the peaks are abundant and

2640 日本外科宝函第28誉第7号

prominent in anacid gastric cancer. These peaks are diminished or disappeared

after dial~·sis. The author has recently proved b~’ using high voltage paper electro-

phoresis and polarograph~· that ninh川lrine stained substances are amino acid and

dialysable low molecular pepticlc.29i It is evident that a large amount of amino acid

is present in the gastric juice of patients with gastric cancer. These data agree

with Onucm et al3°) and G1LLIGAN's31i data obtained l〕yusing paper chromatography.

SUMMARY

1) Five main components were found in the electrophoretic pattern of gastric

juice.

2) The p:1〕tidein t'1e methanol filtrate and dialysate was distributed mainly

on the cathodic side, polarographically represented as a single wave (peptide wave)

and did not stain with P人Sstain.

3) There is a significant difference betw’ecn acid and anacid cases in the elec-

trophoregram and PPgram. In the electrophoregram of acid cases there is a wide

distribution. In the anacid cases the distribution is narrow. The PPgram of the

acid ca巴swa日 characterizedb>・ a single wave on the cathodic side, b:< an atypical

double wave on anodic side and 1Jγa mucoprotein wave on the most anodic side,

that of the anacid ca日C日 wa日 charactcriz仁clii>・ a t~ pical double wave.

4) The PPgram of the anacid cases wa日 changedIηv pepsin digestion to

resemiJ!c the PPgram of acid cases.

5） λlbumin w山 pJlarographically i・cpresented b：＞・ ahigh protein wave with a

low minimum 1nint di百eringfrom a globulin wave. The PPgram of serum was

changed IJ>' pepsin digestion to the peptide pattern.

6) The mucoprotein wave was t~·pical and prominent in acicl non-cancer,

at~·pical or low in acid cancer and not present in anacid caccs.

7) The peptide wave of acid cancer was characterized b）’ a high minimum point.

8) The cobalt maximum on the cathodic side was markedlyア inhibitedin acid cancer.

9) The amount of amino acid was increased in cancer espediallγin anacid cancer.

A(守K:-JOWLEDGE:¥IEi"T

The乱uthorwishes to express his sincere gratitude to Dr. TOKIO s~SAI of the Institute for Chemical Research of K:-・oto Unれでrsityand to Associate Professor Dr. C'rru.rr Kr川JllAof 2nd Surgical Division of Kyoto Uni,・ersity :¥Iedical School for their constant help during this study, and to Professor Dr. Hm:・:o TAKA.IIAT3U and Dr. AKIRA MrzuTANr of the Pathological Division of the Tuberculous Research Institut巴 ofKyoto Unれでrsity,and to Associate Professor Dr. JrN TmmA and Dr. YAsc.,IIr T¥K.11;1 of Centrc.l Laboratory of Kyoto Pniversity Hospital for their kind advice and encourag・ement.

BIBLIOGRAPHY

1 • Crossberg, A. L., Komarov, S A, and Shay, H.: Proteins of Canine Gastric Juice. Elec-trophoretic Characte1包ationand Separation of Fractions. Am. J. Physiol., 165, !, 1951.

STUDIE SON THE GASTRIC JUICE PROTEIN 2641

2) Henning, N., und Kinzlmeier, H.: ElectrophoreseuntersU<:hun邑・enan menschlichen Magen-

saften. Deut. Med. Wschr., 77, (33/34), 998, 1952.

3) Renninふ N., Kinzlmeier, H., und Demling, L. : Uber die electrophoretischdarstellbaren

Proteine normaler und pathologischer l¥Iagensafte. Miinch. Med. Wschr., 95, 423, 1953. 4) Norpoth, L., Surman, T., und Cli〔isges,J.: Uber den Ort der Magenferments in Electroph-

oreeediagram. Klin. Wschr., 31, 1003, 1953.

5）：白 Pugh,B. L., Glass G. B. J., and羽To!f,S.: Electrophoretic Studies of the Mucin Fractions from the Human Gastric Juice. Proc. Soc. Exper. Biol. & Med., 79, 674, 1952.

6〕 Mack,M. H., Wolf, S., and Stern, K. G.: Preliminary Electrophoretic Analysis of Human Gastric Juice. J. Clin. Imest., 32, 862, 1953.

7) Yamakawa, H.: Electrophoretic Analysis of Human Gastric Juice. Jap. J. Gastroenterolo-gy, 51, 484, 1954.

8) Shinoda, K.: Studies on the Paper El2ctrophoresis of Human Gastric Juice. J. Jap. Society Int. Med., -45, 348, 1956.

9) Yoshikawa, H.' Study on Protein of G::tstric .Juices. I. Electrophoretic and Pohrographic

study. Sapporo Med. J., 12, 419, 1957. IO) Kak巴i,M.: Pobro邑:raphicStudies on th号 Prot<?inof the Gastric Juice in Patients with Ga-

stric Disorders with Special Emphasis on the Cancer of the Stomach. Arch. J ap. Chir, 28,

17!, 2603, 1959. 11) Glass, G. B. J., Steph主nson, L., and Rich, M.: Paper-el号ctrophoretic Analysis of Gastric

juice. Fed. Proc., 15, 75. 1956.

12) Mori, G., and Kobλyashi, l¥I. : Practice of Paper Ehctroph川、巴雪is.Nankodo （勺.LTD, Tokyo

Japan, 1956.

13) Akabori, S., and Mizushima, :U. : Chemistry of Protein. Vol I. Kyoritsu Shuppan℃り.LTD.,

Tokyo Japan, 1954. 14) Brezina, M., und Zuman, P.: Die Pol且rographiein der l¥kdizine, Bi'Jchemie and Pharm-

azie. Leipzig, Akademische Verlagsgeselshaft, 1956. 15) Kolthoff, I. M., and Lin邑・ane,J. J .. Pohrography. Second edition. Interscience Publishers,

New York & London, 1952. 16) Homoll王a,J., and Mydlil, V.: Blood Proteins in Infants from a Quantit抗 iveand Qualitative

Point of View. Ann. Paediat., 185, 129, 1955. 17) Glass, G. B. J., Rich, M., and Stephanson, L.: Coinparative Study of Mucoproteins of Human

Gastric Juice and Serum. Gastroenterology, 30, 598, 1956. 18) Glass, G. B. J.: Gastric Atrophy. Its Clinical Significance and Methods of Detection. Am. J.

Digest. Diseases, New series 2, 709., 1957. 19) Glass, G. B. J.: The Deri,・ation and Physiological Significance of Glandular Mucoprotein of

Human Gastric Juice. The Relation of its Secretion to Atrophic Processes in the Stomach. J.

Nat. Cancer Inst., 13, 1013, 1953. 20) Balle-Helares, E.: Recherches experimentales sur la doubl巴 vague de Brdicka. Ann. de

Biol. Clin., No3-4 Mars-Avril, 1956. 21) Sasai, T.: Clinico-experimental Studi巴son th巴 Significanceof the Forms of the Polarog-r-

aphic Protein Double Wave. (I) Changes of the Filtrat巴 Wave(Brdicka)byMeans of Serum

Denaturation. Bull. Inst. Chem. Research, Kyoto. Univ., 34, 321, 1956. 22) Sasai, T., and Egawa, M.: Polarographic Studies on th巴 AlkalineDenaturation of Serum

Protein. (II). Bull. Inst. Chem. Research, Kyoto. Univ., 22, 62, 1950.

23) Sasai, T., Egawa, M., and Usami, H.: Pol引・ographicStudies on the Alkalin巴 Denaturation

of the Serum Protein. (IV). The Relationship Between the Serum Protein v司Taveand their Fraction. Bull. Inst. Chem. Research, Kyoto Univ., 25, 54, 1951.

24) Hata, T., and Matsushita, S.: Biochemical Studies on Sulfhydryl Group. VII. Bull. Research

Inst. Food Science, Kyoto Univ・＇7, 33, 1951. 25) Hata, T., and Matsushita, S. : Biochemical Studies on Sulfhydry 1 Group. VIII. Polaro邑-raphic

Observation on the Enzymic Digestion of Ovalbumin. Bull. Research Instit. Food Science,

Kyoto Univ, 10, 59, 195'.;. 26) Latner, A. L., Ungley, C'. C., Cox, E. ¥'., and R且in巴， L.:Electrophoresis of Human Gastric

2642 日本タ係ヰ宝函第28巻第7号

Juice in Relation to Castle’s Intrinsic Factor. Brit. Med. J., 28, 467, 1953.

27) Glass, G. B. J., and Boyd, L. J .. The three :¥Iain Components of the Human Gastric Mucin:

Dissoh・ed Mucoproteose, Dissolved Mucoprotein, and l¥lucoid of the Gastric Visibl2 Mucus.

Gastroentero!ogy, 12, 821 (part I'I, 835 (part II), 849 (part III), 1949.

28〕 Glass,G. B. J.: Hematopoietic Ac:ti、ityof Glandular Mucoprotein of Human Gastric Juice.

Gastroenterology, 23, 219. 1953.

29) Kakei, :11.. et al: Studies on Gastric Juice Protein. V. Characteristics of the Gastric Peptide

and Tissue Proteins Obsen・ed by Paper-electrophoresis-polarography. Reported at 45th

General Meetings of the Gastroenterological Society of J ap. 1959.

30) .Ohuchi, K., and A－.；、•ataguchi, J. : Free Amino acid in Gastric Juice of Patients with Gastric

or Duodenal Ulcer and Gastric Carcirιma with Special Reference to the Change after

Operation. Tohoku J. exp. Med., 67, 123, 1958.

31) Gilligan, P. R., Moor, J. B., and Warren, S.: Paper Partitio口 Chromatography of Free

Amino Acid and Peptides of ;¥lonnal Human Gastric Juice. J. :¥Tat. Cancer Inst., 12, 657, 1951.

和文抄録

胃液内蛋白の研究

第 2報胃液内蛋白の癒紙電気泳動ーポー

ラログラフ的研究

京都大学医学部外科学教室第2講座（指導：青柳安誠教授）

京都大学医学部内科学教室第 1講座（指導：脇坂行一教授）

箆守

凍結乾燥した胃液について鴻紙電気泳動を行い，そ

の鴻紙を 1糎巾に切って溶出しポーラログラフで蛋白

波を撮って検ベる新しい方法（PPgram）を用いて，

胃疾患特に胃癌の胃液内蛋白像の変化を検索して次の

結果を得た．

1) 胃液蛋白i士電気泳動上5つの分画に分れる．

2) メタノー＇~·泌液及び透析外液内のPeptid巴は泳

動上主として陰極側に伶勤しP E》AS ~色では；~ らずP

ポーラログラフでは一重波を示す．

3) 有酸胃液と無酸胃液とでは泳郵図上でも，PP-

gram上でも明らかに異ったPatternを示す.1'!Pち有

酸では広い泳動図ら無酸では狭い泳動図を示し，ま

たPPg-ramでは，有畿では陰短側で一重波を陽極側

では二重波を示し，特トこIはも陽極側は特異な蛋白波

':¥Iucoprotein Wave）を示した．一方無酸では総て

二重波を示した．

4) Mucoprotein Wave は有酸非癌例で高し特

に十二指腸波疹例で典型的且つ著明でP 胃癌例は低い

か非典型的であり，無骸例は桜p 非癌をとわずMuco・

protein Waveは見られなかった．

5) 無酸のPPgramはp 倹酸胃液蛋白を Pepsinで

消化することによって有酸の PPgramに変えること

が出来た．

6) 有酸の胃癌例では陰極側の PeptideWaveは

Minimum Pointが高く，コベルト極大も特に強く抑

制されるのが特異である．

7) 宵癌胃液内にはアミノ酸の増量を認めた．

以上の結果から，胃癌胃液の PPgram上の特長は

鑑別診断上：G:i主あるものとちえられる．

studies on the gastric juice protein part ii...

Documents