The country of origin:Japan The place of origin: Yamagata Pref.

西瓜（CITRULLUS LANATUS）果提取物

/中文名称

Natural ingredients・ Extracted from watermelon grown in Japan (Yamagata Prefecture)・Suitable for natural cosmetic products

Environmentally conscious・ We use unripe watermelons that were thinned out during crop cultivation・An ecological product making use of watermelons that would otherwise be discarded

Skin improvement effects• Is likely to improve skin smoothness• Increases natural moisturizing factors (NMFs) that moisturize the skin• Reduces redness of the skin by suppressing inflammatory effects

Promoting cell growth・ Promotes growth of normal human cells, thus promoting health skin cell turnover

Anti-aging effects (anti-glycation and anti-oxidation effects)• Suppressing formation of advanced glycation end products (AGEs), which may cause dull, lusterless skin, exerting anti-glycation properties• Anti-oxidation effects

FeaturesWhat is Obanazawa?

Suika (Watermelon) Extract Obanazawa is extracted from unripe watermelons that were thinned out. Differences in terms of extract effect were found between ripe and unripe fruits.

Suika (Watermelon) Extract Obanazawa contains amino acids such as citrulline, GABA, and glutamic acid, as well as malic acid and shikimic acid, which provide peeling effects.

・ Obanazawa is a city located in northeast part of Yamagata Prefecture.・ The climate of the basin, which is characterized by a large temperature difference between night and day, is ideal for cultivation of watermelons, making Obanazawa City the biggest watermelon producer in Japan during the summer. (Based on data from the website of Obanazawa City, Yamagata Prefecture, as of April 2019)

Obanazawa City

YamagataPrefecture

Differences between thinned-out unripe watermelon and ripe watermelon

Cell growth

High

Low

Effect

Low

High

Effect

Anti-inflammatory See inside (Anti-inflammatory effect)

for the testing method

High

Low

Effect

Anti-oxidationTest methodThe peroxyl radical (a type of active oxygen) scavenging activity was measured using the TRAP assay.

Test methodEach sample was added to normal human cells (fibroblasts). After incubating the cells for 3 days, the number of cells was measured.

Based on in-house data

© 2015-2019 KOKEN CO., LTD. C-1-3002-1-02-00

Comparison at 0.5% solid content Comparison at 0.5% solid content

Using extract derived from

Obanazawa Watermelon grown

in Yamagata Prefecture

Ripe

Unripe

Distilled water

Cell growth(%) 120

140

160

100

80

60

40

20

0 Unripe, Suika (Watermelon) Extract Obanazawa

Distilled water Ripe, Suika (Watermelon) Extract

(prototype)Nitric oxide production(%)

120

100

80

60

40

20

0

０ Ripe, Suika (Watermelon) Extract (prototype)

Ripe, Suika (Watermelon) Extract

(prototype)

Unripe, Suika (Watermelon) Extract Obanazawa

Unripe, Suika (Watermelon) Extract Obanazawa

μmol

Tro

lox

eq./g

100

80

60

40

20

0

Using extract derived from

Obanazawa Watermelon grown

in Yamagata Prefecture

Suika (Watermelon) Extract Obanazawa

Safety evaluation Human repeated insult patch test (HRIPT): Negative

Product No. Product name INCi name Other ingredients Package

WME-131 Suika (Watermelon) Extract Obanazawa BG CITRULLUS LANATUS (WATERMELON) WATER, AND BUTYLENE GLYCOL 1 kg FRUIT EXTRACT

WME-121 Suika (Watermelon) Extract Obanazawa PE WATER, AND PHENOXYETHANOL 1 kg

The content in this document is prepared based on documents and experimental data available at the time; however、 the content does not comprise a quarantee. Please note in advance that the content

described in this document may be changed depending on the circumstances.All intellectual property rights including copyrights and design rights of data described here and related documents belong

to Koken Co., Ltd., and any unauthorized reproduction, reprinting or citation is strictly prohibited. Please refer to the MSDS of the product in advance for the safe use of these ingredients.

In addition, care should be taken to comply with regulations in each country on expressions concerning consumer products

-4-14 Koraku, Bunkyo-ku, Tokyo 112-0004, 」apan E-mail: customer@kokenmpc.co.jp TEL: 81—3-3816-3562 FAX: 81-3-3816-35四 www.kokenmpc.co.JpKO KEN CO., LTD.,

KDKEN

Apphcat1ons: Lotions, essences,

C『eams, etc.

Raw material exclusively for cosmetic manufacturing

The cell growth effect was achieved by adding Suika (Watermelon) Extract Obanazawa.Suika (Watermelon) Extract Obanazawa is expected to prove effective in promoting skin cell turnover.

Skin-moisturizing, NMF-related gene expression increased by adding Suika (Watermelon) Extract Obanazawa.

Suika (Watermelon) Extract Obanazawa showed NO production inhibition and was found to have an anti-inflammatory effect.

Hair follicle-related gene expression increased as a result of adding Suika (Watermelon) Extract Obanazawa.

Suika (Watermelon) Extract Obanazawa was found to be effective in improving skin smoothness.

Effects related to natural moisturizing factors (NMFs)

Epidermal keratinocytes Skin fibroblasts

Test methodEach sample was added to normal human cells (epidermal keratinocytes, and skin fibroblasts). After incubating the cells for 4 days, the number of cells was measured.

Test methodSuika (Watermelon) Extract Obanazawa or aminoguanidine hydrochloride solution was added to a solution of dissolved glucose and bovine serum albumin and heated at 60℃ for 48 hours. After heat- solution was diluted 10-fold with water and the fluores- cence intensity (Ex, 370nm; Em, 440nm) was measured.

Test methodSuika (Watermelon) Extract Obanazawa was added to human fibroblasts, and the cells were incubated. The gene expression was analyzed by DNA microarrays.

Test methodImmune cells (RAW264.7 cells, macrophage-derived) were stimulated with lipopolysaccharide to induce an immune response (nitric oxide production).Samples diluted to different concentrations were added to the immune cells. After incubating overnight, nitric oxide production was measured.

Test methodSuika (Watermelon) Extract Obanazawa was added to fibroblasts, and the cells were incubated. The gene expression was analyzed by DNA microarrays.

Test methodThe sample was applied onto the inner side of the forearm once daily, and the skin condition was observed withVisioscan VC98. The skin condition was quantified using dedicated analysis software.

Based on in-house data

Suika(Watermelon)Extract Obanazawa showed concentration-dependent anti-glycation effect. Based on in-house data

0

10

20

30

40

50

60

AG

Promoting cell growth Skin improvement effects

Anti-glycation effect Anti-inflammatory effect Effects related to hair

GlycationA chemical reaction (Maillard reaction) of amino acids or proteins with sugars to form brownish, advanced glycation end products (AGEs).

Effects on skin tissues・Decreases skin elasticity・Causes skin drabness・Decreases skin radiance

Based on in-house data

Based on in-house data Based on in-house data

Smoothness of the skin

Based on in-house data

Period after application (days)

Poorer

Better

0

0.5

1

1.5

2

2.5

0% 0.05% 0.1%

Gene expression

(FLG

)

0

0.5

1

1.5

2

2.5

Viable cell number (%)

Viable cell number (%)

Suika (Watermelon) Extract Obanazawa Suika (Watermelon) Extract ObanazawaSuika (Watermelon) Extract Obanazawa

Suika (Watermelon) Extract ObanazawaAqueous solution containing 0.5% productPurified water

Suika (Watermelon) Extract Obanazawa

5060708090

100110120130140

0.002% 0% (0.2% Blending ratio) (0.8% Blending ratio) (0.2% Blending ratio) (0.8% Blending ratio)

(0.3% Blending ratio) (1% Blending ratio) (3% Blending ratio)

(5% Blending ratio) (10% Blending ratio)

0% 0.05% 0.1%

Suika (Watermelon) Extract Obanazawa(5% Blending ratio) (10% Blending ratio)

0.008% 5060708090

100110120130140

0.002% 0%

0% 0.003% 0.01% 0.03%

0.008%

0%

20%

40%

60%

80%

100%

120%

AGEs formation

suppression ratio (%)

No production ratio

Gene expression (FZD3)

(18% Blending ratio)

Suika (Watermelon) E血actObanazawa

出V,E 0.04

0.02

゜-0.02 . . . -0.04

゜14 28