pBAD24 (control) pLau80 (FtsZ-YFP) pBAD24/ybeM pBAD24/ybiY

5 μm

B

A

Supplementary Data Figure S3. ORFs fsaA, ybiY, aceE, lipA, sdaA, and pflC do not cause substantialfilamentation when overexpressed. The ORFs were expressed from pBAD24 in BW25113 by induction with0.2% L-arabinose in M9 medium. Cells were fixed at OD600 = 0.8. (A) Cell volume distributions of the cellpopulations were determined by Coulter cytometry. The pflC expression strain shows a mild cell division defect ordelay. (B) BW25113 + pBAD24/ybeM and BW25113 + pBAD24/ybiY strains examined by phase-contrastmicroscopy. Expression of ybeM appears to cause a minor effect on cell length, compared to the control and ybiYexpression strains.

1 2 3 4 5 6 Cell volume (µm3)

0

10

20

30

4

0

(%) F

requ

ency

Control (pBAD24)

fsaA

ybiY

aceE

lipA

sdaA

pflC