Suppl. Fig. S1. a) Potato somatic hybrids between C-13 and S. cardiophyllum confirmed with possessing parental bands by SSR marker STM0031, M = 50 bp DNA ladder. b) Chloroplast-type markers ALC1/ALC3 showed cytoplasm types (W/α and W/γ) of somatic hybrids based on 622 bp amplification (Lössl et al. (2000), M = 1 kb DNA ladder. P1= C-13, P2 = S. cardiophyllum, P1+P2 = pooled parental DNA; somatic hybrids #1-4 = Crd-6, Crd-10, Crd-16 and Crd-23, respectively.

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Suppl. Fig. S2. AFLP primer pairs combination (EcoRIACA+MseICTG) confirmed hybridity of the potato somatic hybrids C-13 (+) S. cardiophyllum possessing parental bands. (a) C-13 (215 bp), (b) S. cardiophyllum (162 bp), and (c) somatic hybrid (162 and 215 bp) analyzed on the “3500 Genetic Analyzer” (ABI). Note: because of less peak intensity of 215 bp of somatic hybrid (c image panel) Y-axis scale is reduced to 800 to visualize the peak.

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Suppl. Fig. S3. Ploidy estimation of somatic hybrids and their parents by flow cytometry analysis a) tetraploid common potato cv. Kufri Jyoti (4x), and b) tetraploid somatic hybrid clone Crd-6 (4x).

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Suppl. Fig. S4. Potato somatic hybrids of C-13 (+) S. cardiophyllum: a) pollen viability and b) berry formation of cross between Kufri Girdhari x somatic hybrid (Crd 23)

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