supplementary figure 1 colorimetric analysis of inorganic p by molybdenium staining of hydrochloric...
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Supplementary figure 1 Colorimetric analysis of inorganic P by molybdenium staining of hydrochloric extracts of rice grains in rice grains (non-embryo half). Left: 1A to 1D and 7E to 7H: P standards at amounts of 0.155μg, 0.465μg, 0.93μg, and 1.395μg, respectively; 2A to 2H: Os-lpa-XS110-1; 3A to 3H: Os-lpa-XS110-2; 4A to 4H: heterozygous Os-lpa-XS110-3; 5A to 5E and 6A to 6E: T1 seeds of T-DNA activation tagging line 4A-02500; 7A to 7D:
Dongjin (parental variety of 4A-02500). Right: 8A-8E, P standards at amounts of 0.00, 0.155μg, 0.465μg, 0.93μg, and 1.395μg, respectively; 9A to 9H: XS110; 10A to 10H: Os-lpa-XS110-2; 11A to 11H: homozygous Os-lpa-XS110-3; 12A to 12H, 13A to 13H, 14A to 14H, 15A to 15H, 16A to 16H and 17A to 17H; 18A to 18H, 19A to 19H: T2 seeds from individual T1 plants of 4A-02500 at T2 generation.
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[mV] InsP3 InsP4
InsP5
InsP6
Os-lpa-XS110-3
Xiushui 110
2 4 6 8 10 12 14
retention time [min]
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resp
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[mV]
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InsP6
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InsP6
Os-lpa-XS110-2
Standard inositol phosphates
Supplementary figure 2. HPLC of acid-soluble Ins phosphates in wild type Xiushui 110 and its two LPA mutants. InsP6 is
hexakisphosphate, or phytic acid. InsP3, InsP4, and
InsP5 are tri-,
tetrakis- and pentakisphosphates, respectively.
WTM LPA
R S B R S B
WTM LPA
R S B R S B
Actin
MRP5
bp
400300
300200100
Supplementary figure 3. RT-PCR analysis of OsMRP5 gene using total RNA isolated from root (R), sheath (S) and blade (B) of 14-day old seedlings. M: DNA marker; WT: parent variety ‘Dongjing’; LPA: seedlings of low phytic acid seeds of T-DNA insertion lines at T3 generation. RT-PCR was performed different tissues of. The actin gene was used as control.