swine influenza panel ( 신종플루 ) cdc swine flu test kit incorporates biosearch technologies’...
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Swine Influenza Panel ( 신종플루 )CDC Swine Flu Test Kit Incorporates Biosearch Technologies’ Black Hole Quencher(BHQ) Dye
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T: 02)516-7331 F: 02)516-5123
Responding to a Public Health Emergency
In response to the outbreak of swine flu, or 2009 H1N1, the declaration of a public health emergency by the Secretary of Health and Human Services, and requests by the US Center for Disease Control and Prevention’s (CDC), the US Food and Drug Administration (FDA) issued an Emergency Use Authorization (EUA) for the emergency use of the Swine Influenza Virus Real-time RT-PCR Detection Panel (Swine Flu Test Kit). The CDC readily developed the Swine Flu Test Kit to detect and identify cases of the swine influenza A (H1N1) virus, and is the only FDA-authorized distributor of this molecular diagnostic test to public health and other qualified laboratories.
Diagnosing the Swine Flu - BHQ Dye to Detect Swine Influenza Strains
The Swine Flu Test Kit is a panel of dual-labeled probes and primers for use in real-time PCR to detect swine flu RNA in nasal and/or nasopharyngeal swab specimen. The CDC designed InfA and swInfA (NP gene) probe and primer sets to universally detect all swine influenza A strains as well as a swH1 (HA gene) probe and primer set to universally detect the swine H1 influenza strain. Available to the public online, the CDC posted its protocol of real-time PCR for detecting swine influenza A/H1N1 (CDC REF.#I-007-05), which lists FAM and Biosearch Technologies’ Black Hole Quencher® (BHQ®-1) dye as the official fluorophore and quencher labels for the Swine Flu Test Kit.
As the inventor of the Black Hole Quencher dyes, Biosearch Technologies is the sole manufacturer of the true dark quencher currently used for labeling probes implemented in swine flu diagnostic kits.
Order Information – 미국 CDC 권고 Swine Flu Realtime PCR Primer & Probe VAT 별도
Cat. No Swine Flu Panel 보장량 5'Intern
al3'
Purification
Price
INFA-P-5 Universal Influenza A Probe 5 nmol FAM BHQ-1 Dual HPLC 240,000INFA-F-20 Universal Influenza A Forward Primer 20 nmol RPC 30,000INFA-R-20 Universal Influenza A Reverse Primer 20 nmol RPC 30,000SWINFA-P-5 Swine Influenza A Probe 5 nmol FAM BHQ-1 Spacer 3 Dual HPLC 280,000SWINFA-F-20
Swine Influenza A Forward Primer 20 nmol RPC 30,000
SWINFA-R-20
Swine Influenza A Reverse Primer 20 nmol RPC 30,000
SWH1-P-5 Swine Influenza H1 Probe 5 nmol FAM BHQ-1 Spacer 3 Dual HPLC 280,000SWH1-F-20 Swine Influenza H1 Forward Primer 20 nmol RPC 30,000SWH1-R-20 Swine Influenza H1 Reverse Primer 20 nmol RPC 30,000RNP-P-5 Human RnaseP Positive Control Probe 5 nmol FAM BHQ-1 Dual HPLC 240,000
RNP-F-20Human RnaseP Positive Control Forward Primer
20 nmol RPC 30,000
RNP-R-20Human RnaseP Positive Control ReversePrimer
20 nmol RPC 30,000
한국공식대리점 부경에스엠
Real Time qPCR TaqMan Probe
부경에스엠 www.bookyungsm.co.kr
T: 02)516-7331 F: 02)516-5123
PCR Primer Pairs
TaqMan Probe 구매시 적용
Dual HPLC Purification
Multiplexing Recommendations for Dual-labeled Dark-Quenched Probes & Primers
BHQTM Quencher & Dye Selection Chart
기타 규격 및 자세한 제품 정보는 w w w .biosearchtech.com 에서 확인 하실 수 있습니다
Cat NO Description Scale Yield Cat NO Description Scale YieldDLO-RFB-5
5' FAM / 3' BHQ-1 50nmol 10nmol DLO-TB2-5
5' TAMRA / 3' BHQ-2 50nmol >10nmol
DLO-FB1-5 100nmol >10nmol DLO-TB2-2 200nmol >25nmol DLO-FB1-2 200nmol >25nmol DLO-TB2-1 1µmol >60nmol
DLO-FT-5 5' FAM / 3' TAMRA
100nmol >10nmol DLO-C590B2-
5 5' CAL Fluor Red 590 (TAMRA) / 3' BHQ-2
50nmol >10nmol
DLO-FT-2 200nmol >25nmol DLO-C590B2-
2200nmol >25nmol
DLO-F(T-B1)-2
5' FAM / Internal T-BHQ-1 200nmol >10nmol DLO-Q5B2-5 5' Quasar 570(Cy3) / 3’ BHQ-2
50nmol >10nmol
DLO-F(T-B1)-1
/ 3’ C3 1µmol >30nmol DLO-Q5B2-2 200nmol >25nmol
DLO-TET-5 5' TET / 3' TAMRA
50nmol >10nmol DLO-Q6B2-5 5' Quasar 670(Cy5) / 3’ BHQ-2
50nmol 10nmol DLO-TET-2 200nmol >25nmol DLO-Q6B2-2 200nmol >20nmol
DLO-TEB1-5 5' TET / 3' BHQ-1
50nmol >10nmol DLO-Q7B2-5 5' Quasar 705(Cy5.5) / 3’ BHQ-2
50nmol >3nmol DLO-TEB1-2 200nmol >25nmol DLO-Q7B2-2 200nmol >10nmol DLO-CGB1-5 5' CAL Fluor Gold 540 (TET,
JOE) / 3' BHQ-1
50nmol >10nmol DLO-CAB2-5 5' CAL Fluor Red 610 (TEXAS RED)
/ 3' BHQ-2
50nmol >10nmol
DLO-CGB1-2 200nmol >25nmol DLO-CAB2-2 200nmol >25nmol
DLO-HB1-5 5' HEX / 3' BHQ-1
50nmol >10nmol DLO-C635B2-
5 5' CAL Fluor Red 635 (LC Red 640)
/ 3' BHQ-2
50nmol >3nmol
DLO-HB1-2 200nmol >25nmol DLO-C635B2-
2200nmol >10nmol
DLO-COB1-5 5' CAL Fluor Orange 560 (VIC,HEX) / 3' BHQ-1
50nmol >10nmol DLO-B2P6-2 5’ BHQ-2 / 3’ Pulsar 650
200nmol >10nmol DLO-COB1-2 200nmol >25nmol DLO-B2P6-1 1µmol >40nmol
Cat NO Description Scale YieldP2-5
Primer Pair (Cartridge Purified, up to 30 base)
50nmol >11nmol P2-2 200nmol >45nmol P2-1 1µmol >225nmol
InstrumentCalibration required
Multiplexing Degree
DYE 1 DYE 2 DYE 3 DYE 4 DYE 5
Prism 7700 YES Duplex FAM CAL Fluor Gold 540 SuperROX Prism 7900 YES Duplex FAM CAL Fluor Gold 540 SuperROX Prism 7000 YES Duplex FAM CAL Fluor Gold 540 SuperROX Prism 7300 YES Duplex FAM CAL Fluor Orange 560 SuperROX Prism 7500 YES 4-Plex FAM CAL Fluor Orange 560 TAMRA SuperROX Quasar 670
iCycler iQ YES 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red
610Quasar 670
iQTM 5 YES 5- Plex FAM CAL Fluor Gold 540CAL Fluor Red
590CAL Fluor Red
610Quasar 670
SmartCycler YES Triplex FAM CAL Fluor Orange 560CAL Fluor Red
635
SmartCycler II YES 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red
610Quasar 670
Rotor-Gene 3000
NO 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red
610Quasar 670
Rotor-Gene 6000
NO 5- Plex FAM CAL Fluor Orange 560CAL Fluor Red
610Quasar 670 Quasar 705
MasterCycler YES Duplex FAM CAL Fluor Gold 540 Opticon 2 YES Duplex FAM CAL Fluor Orange 560
Chromo 4 YES 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red
610Quasar 670
MX3000P NO 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red
610Quasar 670
MX4000 NO 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red
610Quasar 670
LightCycler 1.2 YES Duplex FAM Pulsar 650 LightCycler 2.0 YES Triplex FAM CAL Fluor Red 610 Pulsar 650
LightCycler 480 YES 4-plex FAM CAL Fluor Orange 560CAL Fluor Red
610Quasar 670
Determined through research at Biosearch
Determined through research of a collaborator
Predicted based on instrument specifications