Sympathetic denervation in the mice

~ My life in San Diego

生科 04 楊慧儒 891644

2002.12.06

劉新光 教授 John H. K. Liu, Ph.D.

the regulation of intraocular pressure An elevated intraocular pressure ─

primary open-angle glaucoma the circadian (24-hour) rhythms of intraocular pr

essure and related ocular functions. Human study─ eye drop for glaucoma

Summer research

Superior cervical ganglionectomy Way to measure the pupil size of the mice Test of eye drop on postsurgical mice

Superior Cervical Ganglion (SCG)

Superior Cervical Ganglion (SCG)

Sympathetic nerve─change in pupillary reflex Removal or sectioning of this part can reduce th

e circadian IOP elevation The outcome of removal of SCG or cutting the c

ervical sympathetic trunk will mimic human Horner’s syndrome─drug test

PAST~ Palpebral ptosis and become irreversible from 20th hr Palpebral retraction

Pharmacology of the sympathetic nervous system

hydroxyamphetamine

Drug to Test Horner’s Syndrome

Hydroxyamphetaine(HA)

& Tyramine(TA)

Dilation→

no dilation

Postganglionic

Cocaine(C) Dilation→

No dilation

Pre-,postganglionic

Epinephrine(E) No dilation→

Dilation

Postganglionic

Ganglionectomy

Mouse(C57BL/6)

Male, 30g, 4 mice

Anesthesia

(Ketamine+Xylazine)

Fix it on the bench and preparation of instruments

Surgery

Toe & tail

Ganglionectomy

Shave Incision Find SCG Cut SCG (right side) Suture back the fats &

the skin

Ganglionectomy

Measurement of pupil size

Anesthetized Ketamine pentobarbital

Conscious Tube Cone

Ruler

Eye Drop Test

Solution (saline) 1 micro-liter, twice, on each eye Record every 5-10mins Experimental time: 70mins

M: mouse number M0: normal mouse (day): the day after surgery

Result- Hydroxyamphetamine & Tyramine

Conscious Ketamine Pentobarbital

HA (1%) M1(9),M2(8),

M0

M1(7),M2(6) M1(10)

HA (0.1%) M2(7)

TA (5%) M1(15),M2(14),M3(7),M0

TA (1%) M1(16),M2(15),M3(8)

TA (0.1%) M1(17),M2(16),M3(9)

Result- Cocaine

Conscious Pentobarbital

Cocaine M1(18),

M2(17),

M4(1,3),M0

M2(9)

Result- Epinephrine

Conscious

Epinephrine 0.1% M1(10)

M2(13)

M3(6,10,15)

M4(8)

M0

Discussion

Compare to rabbits: Cocaine─ 5~30mins Hydroxyamphetamine─ 30~45mins

Cocaine, hydroxyamphetamine, and tyramine work on rabbits but not mice.

Only using epinephrine at certain day after surgery(10th-15th days) can have significant result to confirm the success of surgery.

Furthermore

Increase the number of experiment Find a critical way to measure the pupil size of

mice Electrode stimulation The mechanism of drug effect

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