target exon 1 atg
TRANSCRIPT
Plasmid ID Gene targeted Talen Sequence (Left talen/spacer/right talen)
binding sequences
tag MethodSegment I. Segment II. Segment III. Segment IV.
SD_14 SORL1_ATG-Left Set1 T GCAGTAGCGTTCGCC cgaacatggcgacac GGAGCAGCAGGAGGG A GCAG TAGC GTTC GCC GFP Musunuru-
Cowan
SD_15 SORL1_ATG-right Set1 T GCAGTAGCGTTCGCC cgaacatggcgacac GGAGCAGCAGGAGGG A CCCT CCTG CTGC TCC RFP Musunuru-
Cowan
target Exon 1 ATG
parental GFP control
TALEN SORL1
16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 17 18 19
WT homKO
het KO
het KO
Primer for screening
TALsorlafw: AGAAAGTGCGCGAAAGGGAC 230 bp vor ATG
TALsorlarev: TTGCAAAACTGCTCACCTGTC 301 bp nach ATG
Produkt: 531 bp
clone 17 – ATG KO
clone 6 – ATG WT
250
50
SORLA
α-tubulin