the role of mirna in cancer
TRANSCRIPT
Follow up miRNA In Cancer
Supervised by : Dr.Moein
Presented by : Mohsen Koolivand MSc student of Clinical Biochemistry
September 2015
Hormozgan University of Medical Sciences Faculty of Medicine Bandar Abbas, Iran
Contents:
Introduction Of Cancer
Investigate The Molecular Mechanisms Of Cancer
Introduction of miRNA
miRNA Role In Cell
The Use Of miRNA For Diagnosis and Treatment Of Cancer
Conclusion
Resources
Introduction
M.Koolivand MSc 4
Cancer definition
M.Koolivand MSc 5
The Molecular Mechanisms Of Cancer
• Genomic instability and mutations• Evading growth suppressors• enabling replicative immortality• Activating invasion and metastasis• tumor promote inflammatory• Angiogenesis• evading immune destruction• sustaining proliferative signaling• Reprogramming energy metabolism
M.Koolivand MSc 6
EPIDEMOLOGY OF CANCER
M.Koolivand MSc 7
Introduction of miRNA
MicroRNAs (miRNAs) are endogenous small noncoding RNAs (20 – 23
nucleotides) that negatively regulate the gene expressions at the
posttranscriptional level by base pairing to the 3' untranslated region of target
messenger RNAs.
Hundreds of miRNAs have been identified in humans and evolutionarily
conserved from plants to animals.
It is revealed that miRNAs regulate various physiological and pathological
pathways such as cell differentiation, cell proliferation, and tumoriogenesis.
M.Koolivand MSc 8
M.Koolivand MSc 9
miRNA Role In Cell
Cell Cycle
Differentiation Apoptosis
Cell MetabolismDevelopment
M.Koolivand MSc 10
M.Koolivand MSc 11
M.Koolivand MSc 12
M.Koolivand MSc 13
M.Koolivand MSc 14
Development & Differentiation
M.Koolivand MSc 15
M.Koolivand MSc 16
Apoptosis
Apoptosis describes the orchestrated collapse of a cell characterised
by membrane blebbing, cell shrinkage, condensation of chromatin,
and fragmentation of DNA followed by rapid engulfment of the
corpse by neighbouring cells.
It is distinguished from death by necrosis by the absence of an
associated inflammatory response.
M.Koolivand MSc 17
M.Koolivand MSc 18
M.Koolivand MSc 19
miRNA Role In Cancer
1- miRNAs are generally downregulated in cancer
2- Mechanisms for miRNA deregulation and escape from miRNA- mediated repression
3- miRNA regulation of key cancer-related pathways
M.Koolivand MSc 20
M.Koolivand MSc 21
M.Koolivand MSc 22
M.Koolivand MSc 23
M.Koolivand MSc 24
M.Koolivand MSc 25
Examples
M.Koolivand MSc 26
M.Koolivand MSc 27
M.Koolivand MSc 28
M.Koolivand MSc 29
The Use Of miRNA For Diagnosis and Treatment Of Cancer
Circulating miRNAs in cancer: from detection to Therapy
Detection methods to quantify serum miRNAs in cancerpatients
Methods
qRT-PCR
TaqMan miRNA array
Microarray
TaqMan low density array
NanoString-nCounter microRNA assays
LNA™
M.Koolivand MSc 30
Example
Metastatic breast cancer lung cancer
Pancreatic CancerHepatocellular Carcinoma
M.Koolivand MSc 31
M.Koolivand MSc 32
Conclusion
Progresses on the biology of miRNAs greatlyenhance our understanding of their functions with respect to basic mechanisms of oncogenesis and diagnostics and prognostics.
cell-free miRNAs might play an increasing role as non-invasive tools for the detection of cancer in early stages and as biomarker to monitor prognosis and response to therapy. However, knowledge on the biological functions of extracellular miRNAsis in its infancy.
M.Koolivand MSc 33
1.http://www.biooncology.com/molecular-causes-of-cancer 2. https://www.qiagen.com3. http://www.nature.com/4. http://www.ncbi.nlm.nih.gov/pubmed 5. http://www.sciencedirect.com6. https://scholar.google.com 7. http://www.slideshare.net/ 8. https://www.linkedin.com/ 9.http://wikipedia.org
مهدوی. 10 مجید مولکولی و سلولی شناسی زیست11. María José Bueno, Marcos Malumbres, Cell Division and Cancer Group, Spanish National Cancer
Research Center (CNIO), Madrid, Spain, MicroRNAs and the cell cycle
Resources:
M.Koolivand MSc 34
12. Martin D. Jansson, Anders H. Lund, Biotech Research and Innovation Centre and Centre for Epigenetics, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen,
Denmark, MicroRNA and cancer13. Shan Deng,1 George Adrian Calin,4 Carlo M. Croce,5 George Coukos1-3 and Lin Zhang1,2,
1Center for Research on Early Detection and Cure of Ovarian Cancer; 2Department of Obstetrics and Gynecology; 3Abramson Family Cancer Research Institute; University of Pennsylvania; Philadelphia, Pennsylvania USA; 4Department of Experimental Therapeutics; Department of Cancer Genetics; University of Texas; MD Anderson Cancer Center; Houston, Texas USA; 5Departments of Molecular Virology; Immunology and Medical Genetics; Ohio State University; Columbus, Ohio USA, Mechanisms of microRNA deregulation in human cancer
14. Fazli Wahid a, Adeeb Shehzad a, Taous Khan b, , You Young Kim a, , a School of life ⁎ ⁎Sciences and Biotechnology, College of Natural sciences, Kyungpook National University, 1370 Sangeok-dong, Buk-ku, Taegu 702-701, Korea b Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, Pakistan, MicroRNAs: Synthesis, mechanism, function, and recent clinical trials
15. Minlee Kim1,2 and Frank J Slack2, MicroRNA-mediated regulation of KRAS in cancer16. Jingcao Huang1,2, Hui Lyu1, Jianxiang Wang2, Bolin Liu1, 1Department of Pathology, School of Medicine, University of Colorado Anschutz Medical Campus,
Aurora, CO, USA; 2State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China
M.Koolivand MSc 35
17. Benjamin L Jackson1*, Anna Grabowska1 and Hari L Ratan1,2, MicroRNA in prostate cancer: functional importance and potential as circulating biomarkers
18. Wen-Tao Wang and Yue-Qin Chen*, Circulating miRNAs in cancer: from detection to therapy
19. Srivatsava Naidu, Peter Magee and Michela Garofalo*, MiRNA-based therapeutic intervention of cancer
20. Veronica Balatti*, Yuri Pekarky and Carlo M Croce, Role of microRNA in chronic lymphocytic leukemia onset and progression
21. Ingrid Garajová,1,2 Tessa Y. Le Large,1 Adam E. Frampton,3 Christian Rolfo,4 Johannes Voortman,1 and Elisa Giovannetti1,5, Molecular Mechanisms Underlying the Role of MicroRNAs in the Chemoresistance of Pancreatic Cancer
22. M Jovanovic1,2 and MO Hengartner1,, 1Institute of Molecular Biology, University of Zurich and ETH Zurich, Zurich, Switzerland and 2PhD Program in Molecular Life Sciences, University of Zurich and ETH Zurich, Zurich, Switzerland, miRNAs and apoptosis: RNAs to die for
23. Dr. Manash K. Paul,Department of Biology,Indian Institute of Science Education and Research,Mohali; www.iisermohali.ac.in, Cancer and Therapeutics
24. Webinar, February 23rd , 2012, Adam Baker, PhD. [email protected] , microRNA discovery and biomarker development in clinical samples
25. Kaveri Sidhu†, Neetu Rohit Kapoor†,Vijaya Pandey† andVijay Kumar* Virology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi,
India, The “macro” world of microRNAs in hepatocellular carcinoma
M.Koolivand MSc 36
26. MicroRNAs in Cervical Cancer: Evidences for a miRNA ProfileDeregulated by HPV and Its Impact on Radio-ResistanceAbraham Pedroza-Torres 1,7, Eduardo López-Urrutia 1,2, Verónica García-Castillo 2,Nadia Jacobo-Herrera 3, Luis A. Herrera 4, Oscar Peralta-Zaragoza 5,César López-Camarillo 6, David Cantú De Leon 4, Jorge Fernández-Retana 1,2,Jorge F. Cerna-Cortés 7 and Carlos Pérez-Plasencia 1,2,8,*27. QIAGEN GeneGlobe Pathways Molecular Mechanisms of Cancer 28.
M.Koolivand MSc 37
Thanks For Your Attention