theoretical analysis - edgeedge.rit.edu/edge/p15080/public/final documents/p15080 poster - final...
TRANSCRIPT
Key w = Wall Shear Stress
μ = Dynamic Viscosity
u/ y = Velocity Gradient
Q = Volumetric Flow Rate
b = Channel Width
h = Channel Height
Design Concept Complete System
Growth Chamber
1. Peristaltic Pump 2. Tubing 3. Barbed Fitting 4. Stopcock 5. Threaded Luer
6. Polycarbonate Lid 7. Growth Chamber 8. Scratch Guard 9. Glass Slides
10. Bolt/Nut 11. Growth Media 12. Media Reservoir 13. Lid 14. Syringe Filter
Conclusions
Final Product
• Dynamic flow chamber is mechanically and biologically sound
• Flow functionality of the device is compatible only with endothelial cell types
• Device is ready to be used in a teaching laboratory setting
Recommendations • Design of a standalone environmental chamber
in place of a laboratory incubator • Upgrade to a digital pump for more accurate
control of flow rates and shear stresses • Use of an alternate chamber material less
prone to deflection during precision machining to hold tighter tolerances
Theoretical Analysis
COMSOL Modeling of Wall Shear Stress COMSOL Modeling of Flow Rates in the Chamber
Wall Shear Stress
Between Parallel Plates
General Wall Shear Stress
Results
Oriented Bovine Aortic Endothelial Cells, 10x
Seeded Slide within Growth Chamber
1. 1” Bolts 2. Scratch Guard 3. Aluminum Body 4. Aluminum Insert
5. Slide Gaskets 6. Seeded Slides 7. Lid Gasket 8. Polycarbonate Lid
9. Washers 10. Wing Nuts 11. 3/4” Bolts
𝜏𝑤 = 𝜇𝜕𝑢
𝜕𝑦 𝜏𝑤 =
6𝜇𝑄
𝑏ℎ2
System Operating within an Incubator Cell Observation on Microscope
Engineering
Requirement
Number
Function Specification Unit of MeasureMarginal
Value
Ideal
ValueExp. Results
ER5 System Operation Shear Stress Dynes/cm2 0-20 <=20 10
ER9 Ease of Use Assembly Time Minutes <180 120 27.11
ER16 Functionality Cell Growth Cell Density 70%-100% 85% 85%
ER19 Cost Cost of Unit U.S. Dollars ≤1550 0 1000
Core Customer Criteria Customer
Requirement
Number
Customer Requirement Description
CR2 Maintain sterility of the system
CR5 Allows adjustment of flow rate for control of shear stress (0 to 20 dynes/cm^2)
CR7View cells under the following microscopes:
Fisher Scientific MicroMaster Cat. No.12-575-250, Leica DM IL LED Fluo, EVOS® XL AMEX1000.
CR8 System can be assembled in 30 min by a 3-5th year BME student
CR9 Necessary hardware can be run through the autoclave between uses
CR11 Ability to operate with endothelial cell types "CPA 47" and "BCE C/D-1b"
CR14 Maintain functionality within tolerances for minimum of 5 years
CR19 No part of the device can be cytotoxic
Team P15080 From left to right:
Collin Burkhart (ME), Sarah Tran (ME), Robert Repetti (BME), Katelyn Busse (BME), Michelle Garofalo (ME), Morgan Stoessel (BME)
Special Thanks to: Dr. Jennifer Bailey, Dr. Daniel Phillips, Gerald Garavuso,
the Brinkman Lab, and the Biomedical Engineering Department
Current cell culturing techniques used for education and research typically grow cells under static conditions. While this is suitable for some applications, it is not an accurate representation of how cells grow within the body. The purpose of this project is to design a system that allows cell culturing to be performed in a dynamic environment, better modeling cell growth under in vivo conditions.
Project Purpose