Tokyo Medical and Dental University , Tokyo, Japan, 2000-2005

Ginsenoside Re, a Main Phytosterol of Panax ginseng,Activates Cardiac Potassium Channels via a Nongenomic

Pathway of Sex Hormones

Chang-Xi Bai, Asami Kaihara, Eri Ozaki, Takashi Kawano,Yutaka Nakaya, Muhammad Awais, Moritoshi Sato, Yoshio Umezawa, and

Junko Kurokawa

Department of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University

MOLECULAR PHARMACOLOGY Vol. 70, No. 6

Copyright © 2006 The American Society for Pharmacology and Experimental Therapeutics

28134/3156556

Mol Pharmacol 70:1916–1924, 2006

Both panaxadiols and panaxatriols activate IKs.

NO produced by eNOS, but not nNOS, is responsible for IKsenhancement by ginsenoside Re.

Signaling cascade of IKs enhancement by ginsenoside Re. A–C,effects of SH-6 (A), an Akt inhibitor, PP2 (B), a c-Src inhibitor, and

wortmannin (C), a PI-3 kinase inhibitor, on ginsenoside Re-induced IKsenhancement.

Effects of preincubation with various blockers on ginsenoside Re (3 M)-induced IKs enhancement

Involvement of sex hormone receptors on IKs enhancement by ginsenosides

Effects of ginsenoside Re on Akt phosphorylation

Ginsenoside Re binds to the human AR, ER, and PR

Effects of ginsenosides on proliferation of MCF-7 and LNCaP

Effects of ginsenoside on recruitmentof coactivator examined by

FRET experiments

Nontranscriptional Regulation of Cardiac Repolarization

Currents by Testosterone

Chang-Xi Bai, MD, PhD; Junko Kurokawa, PhD; Masaji Tamagawa, BE;Haruaki Nakaya, MD, PhD; Tetsushi Furukawa, MD, PhD

Department of Bio-informational Pharmacology , Medical Research Institute, Tokyo Medical and Dental University

Effects of testosterone on APD

Effects of testosterone on membrane currents

Dose-dependence of ICa,L suppression and IKsenhancement by testosterone

Effects of nisoldipine and chromanol 293B on APD shortening

Role of NO in testosterone-induced APD shortening, ICa,L suppression, and IKs enhancement

Effects of inhibitors of the signal molecules in thenongenomic pathway of testosterone

Phosphorylation of Akt and NOS3 bytestosterone

Effects of testosterone on isolated Langendorff-perfused hearts

Proposed schematic model for regulatory mechanismof testosterone on ICa,L and IKs

Role of Nitric Oxide in Ca2 Sensitivity of the SlowlyActivating Delayed Rectifier K Current in

Cardiac Myocytes

Chang-Xi Bai, Iyuki Namekata, Junko Kurokawa, Hikaru Tanaka, Koki Shigenobu, Tetsushi Furukawa

Department of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University

Effects of A23187 on [Ca2]i, APD, and IKs

Effects of IKs blocker and ICa,L blocker onA23187-induced APD shortening

NO and NOS3 play a role in the A23187-induced APD shortening and IKs enhancement

CaM, but not Akt, is involved in A23187-induced IKs enhancement and APD shortening

Effects of A23187 on interactions of NOS3 with Cav-3 and CaM

Effects of digoxin and rise in [Ca2]o on[Ca2]i, APD, and IKs

The University of Oklahoma Health Sciences Center2005-2009

Activation of TRPP2 through mDia1-dependent voltage gating

Chang-Xi Bai1, Sehyun Kim1, Wei-PingLi1,3, Andrew J Streets2, Albert CM Ong2

and Leonidas Tsiokas1,*

Department of Cell Biology, University of Oklahoma Health SciencesCenter, Oklahoma City, OK, USA

The EMBO Journal (2008) 27, 1345–1356 | & 2008 European Molecular Biology Organization | All Rights Reserved 0261-4189/08

www.embojournal.org

&2008 European Molecular Biology Organization The EMBO Journal VOL 27 | NO 9 | 2008

THE

EMBO

JOURNAL

Colocalization of mDia1 and TRPP2 at the plasma membrane of LLC-PK1 cells

Voltage-dependent block of endogenous TRPP2 by mDia1 in LLC-PK1 cells

Voltage-dependent block of TRPP2 by activated mDia1

Structure–function analysis of mDia1

Voltage-dependent gating of TRPP2 by purified mDia1 in inside-out patches

Activation of TRPP2 by EGF through mDia1-dependent gating

Proposed scheme of the mDia1-dependent regulation of TRPP2

Formation of a new receptor-operated channel by

heteromeric assembly of TRPP2 and TRPC1 subunits

Chang-Xi Bai1*, Aure´lie Giamarchi 2*, Lise Rodat-Despoix 2, Franc¸oise Padilla 2, Tamyra Downs1,

Leonidas Tsiokas1+ & Patrick Delmas 2+

EMBO reports

scientific report

Figure 5. Co-localization of TRPP2 and TRPC1 in primary cilium and association in rat kidney membranes(A) LLC-PK1 cells were stained with a monoclonal antibody to acetylated a-tubulin (611B) (red) or rabbit polyclonal antibody to TRPC1 (green). mIMCD3 cells were stained with a mouse polyclonal antibody to TRPC1 (1F1) (red) or rabbit polyclonal antibody to TRPP2 (green). ( B) Mouse pre-immune serum (lane 2) or mouse polyclonal a-TRPC1

(lane 3) was used to immunoprecipitate endogenous TRPC1. Lane 1: 100 µg kidney membrane lysates were immunoblotted with chick en a-TRPP2.

199

1 2 3

TRPP2

lysate

s (

inp

ut)

Pre

-im

mu

ne

a-T

RP

C1

A B

a-tubulin TRPC1 merge

TRPC1 TRPP2 merge

LL

C-P

K1

mIM

CD

3

128

85