a

Total: Abl

Myc

P-TyrIP:Flag

Flag-Myc WT

Flag-Myc 5YF

+ ++

Abl + +

bGST

GST-Myc 1-262

+ +-Abl

Total:

P-Tyr

GST

+ -+- +-

GST

GST Myc 1-262

GST Myc 1-262Abl-

----

-

-

++--

+ + --

- +--

Bcr-Abl

Flag

P-Tyr

Flag

Flag-Myc WT

Flag-Myc Y74F

Bcr-Abl

Total

IP Myc

c

Bcr-Abl

pY74

Myc

Vinculin

Myc WT

Myc 5YF

+ + +Bcr-Abl+

+

-

+ - -

----

-

-

Total

Myc T58A + ----d

Sanchez-Arévalo et al. Fig. S2