utilizing powerpoint effectively

Putting PowerPoint to Work for You

A 10 Step Program of sorts…

Admitting that there is a problem …




Step 1: Hold up your end with “compelling material”.

“Substance” vs. “Style”


“Substance” vs. “Style”


HRC PH

X



Step 1: Hold up your end with compelling material.

Step 2: Keep it simple.


Ooooouuuuu….

Aaaaaahhhhhhhhhhhhhhh…

LOOK @ ME…

I am Special… with a capital S…


If you utilize slide transition animations:

Be consistent from start to finish

Shy away from sounds

And…





Step 3: Minimize the number of slides & text.


The competition is rough...

http://www.nature.com/neuro/journal/v10/n8/index.html

http://www.ok-magazine.com/current_issue.php


Then…

Constructing a PowerPoint presentation…is like preparing for a backpacking expedition.

•Plan ahead. •Review your goals.•Plan for emergencies.


TEXT: Less > More...

Sentences :o(

Bullet Points :o)

Consistent Style

Key Idea Only


6 X 6


Where are we going and why?

Outline the itinerary.

Give some background info.

Get the tour bus moving.

Don’t be afraid to pull the bus over.

Bring it back to the beginning.

Think APA writing format…

Think tour bus…





Step 3: Minimize the number of slides and text.

Step 4: Don’t parrot PowerPoint.







Step 5: Time your remarks.


About 100 words per minute…

Repeat questions posed to you…

It is OK to pause before you answer a question…

Keep a healthy pace but keep in mind that if the audience senses that you are rushing...

Time your remarks…








Step 6: Give it a rest.









Step 7: Careful with fonts and backgrounds.

Some fonts are:

– really ugly– hard to read and annoying– suitable for comics– More science fiction than

scientific

Careful with fonts styles…



Arial

Sans Serif

Times New Roman

The Big 3


Oh so temping…

You don’t design ads for



Backgrounds

Ask yourself:•Does it help the presentation?

•Work with my text & graphics?

•Does it seem out of place?

•Will they wonder if I forgot to take my meds?

Making PowerPoint work for you.

A 10 Step Program of sorts


The competition for the audience’s attention is rough.

Don’t give them another excuse to tune you out!

http://www.nature.com/neuro/journal/v10/n8/index.html

http://www.ok-magazine.com/current_issue.php

Are You Kidding Me?


The beauty of “white space”…

Clean & crisp

Text pops off the page

Keeps the focus on your message


http://lifesciences.umaryland.edu/downloads/GPILS%20Folder.pdf


Final word on backgrounds …

Ok, white space is not always best

Think contrasting colors

Think readability & clarity

Then… Try it out









Step 7: Careful with backgrounds and fonts.

Step 8: Import images, graphics and other media


Thank you, Al Gore …


Got images?


RE: Graphs...

2-D vs. 3-DAdios grid-linesContrast with colorWider is betterProvide the punch-line

Note the increase in mean GRE scores

1050

1100

1150

1200

1250

1300

1350

2006 2007

Biochemistry

Microbiology

MolecularMedicine

Neuroscience

For entering studentsN = 36 in 2006N = 33 in 2007


It took centuries to decipher the Rosetta stone…

Your audience has 1 minute…


Good slide vs. poor slide…

Sometimes…

the difference is subtle…

• Fatty acid-binding protein (FABPs) belong to a family of intracellular lipid binding proteins of low molecular mass (14–15 kDa) with the putative general function of lipid trafficking (1).

• The precise physiological functions of these proteins are as yet unclear, but it is hypothesized that they are important in intracellular transport and targeting of FA to specific membranous organelles and metabolic pathways.

• Intestinal fatty acid-binding protein is abundantly produced in the enterocyte, where a second fatty acid-binding protein (FABP), liver FABP (LFABP), is also highly expressed (2)

Fatty Acid binding proteins

http://www.jbc.org/cgi/content/full/281/20/13979#REF1

http://www.jbc.org/cgi/content/full/281/20/13979#REF2

• family of intracellular lipid binding proteins

• low molecular mass (14–15 kDa)

• putative function in intracellular lipid trafficking

• tissue specific expression of different FABPs• intestine expression both L-FABP and I-FABP

• the functional roles of these FABPs in lipid absorption are as yet unclear

• may regulate FA traffic among organelles

Fatty Acid Binding Proteins (FABPs)

• family of intracellular lipid binding proteins

• low molecular mass (14–15 kDa)

• function in intracellular lipid trafficking

Fatty Acid Binding Proteins (FABPs)


It a scary world out there…

In our lecture halls…

Neuromuscular Junction(Motor Neuron to Muscle Fiber Synapse)

Freeze fracture cleaves membranes in hydrophobic plane of bilayer

Electron micrograph

Vesicle Trafficking and ExocytosisLearning Objectives:1. Systems used to study vesicle fusion & exocytosis

a. Yeast secretory mutantsb.* C. elegans movement mutants (uncoordinated)c.* Drosophila mutantsd. Cell free Golgi vesicle trafficking assaye. Protein purification, antibodies, interacting proteins

[synaptic preps: Torpedo, brain]f. Synaptic preparations for electrophysiology

- squid giant synapse• SNARE hypothesis

a. NSF, SNAPs and SNAP Receptors• Identity of t- and v- SNARES• SNARE zipper mechanism (postdocking)• Role of NSF & SNAPs

• Very rapid, Ca-dependent exocytosis at synapses

• Ca sensor: Synaptotagmin

Unsaturated fatty acids inhibit 2nd phase.

Sphingolipid and cholesterol cause distinct phase to form in pure lipid mixtures containing

phospholipid with saturated fatty acid chains

Mix DOPC (unsat’d) or DPPC (sat’d) at different ratios, with sphingolipid +/- cholesterol, at 23oC or 37oC. Assay by fluorescence quenching of marker that only sees sol phase lipid. Phase separation indicated by break in slope of quenching curves.

See 2nd phase at 23oC with increasing DPPC .

Need cholesterol to obtain 2nd phase at 37oC.

Cholesterol promotes 2nd phase formation

Human Genome Project1990-2003

HAPMAP2002 –

http://www.hapmap.org/

ENCODE Project 2003 -

ENCyclopedia of DNA Elements:identify all functional elements in the human genome sequence

compare the genetic sequences of different individuals to identify chromosomal regions where genetic variants are shared.

3: Examine Region

Chromosome-wide summary data is

shown in overview

Default tracks show HapMap genotyped SNPs, named genes

from Entrez, and alternative mRNA splicing patterns

Electricalactivationof skeletal

muscle forceproduction

Single stimulus, single twitch

Multiple stimuli, unfused or fused tetanus

Prolonged depolarization,long contracture then relax( Not physiological )

Force

Force

Force

AP

Time


There is hope.

Overview

1. ESI mass spectrometry

2. Protein-Protein Interaction and Pathways

high voltagehigh voltage

samplesample

vv11

m1

vv22

m2

vv33

m3

m1 m2 m3

detectordetector

drift regionlaserlaser

Matrix-assisted Laser Matrix-assisted Laser Desorption/Ionization (MALDI)Desorption/Ionization (MALDI)

Time-of-Flight (TOF) AnalyzerTime-of-Flight (TOF) Analyzer

MALDIMALDI

•Differentiation - need to generate multiple cell types•Morphogenesis - cells need to be spatially organized

How do you generate an organism from a single cell?

in situimmuno

How do we experimentally identify a tissue or cell type?

1. Histology

smooth muscle nerve cells










Step 8: Import other images, graphics and other media.

Step 9: Distribute handouts at the end.










Step 8: Import other images, graphics and other media.

Step 9: Distribute handouts at the end.

Step 10: Edit ruthlessly before presenting.


Get acquainted with the F7 key.

Employ the eyes of others.

If your not sure… cut it out.

Don't speak without rehearsing.

Even Seinfeld rehearses and…

He has nothing to say…

Promoting Excellence in Biomedical Education

lifesciences.umaryland.edu

utilizing powerpoint effectively

Design