vere chip tm
DESCRIPTION
Biochip VerechipTRANSCRIPT
VereChipTM
Khilda Afifah23214316
Mengintegraskan Biochips dengan silicon heaters, temperature sensors and
miniaturized 25μl volume allow the PCR module
Product
VereFlu untuk mendeteksi Influenza A: H1, H3, H5, H7, H9 with subtype ID’s of H1N1, A/H1N1-
2009, H3N2, H5N1 and H9N2 and Influenza B.
VereMTB to detect, differentiate and identify: 10 different mycobacterium strains with special
emphasis on Mycobacterium tuberculosis Complex (MTBC) and its Resistance to Rifampicin
and/or Isoniazid from Pulmonary Clinical Specimens or Cultivated Samples (MDR-TB)
VereTrop to detect, differentiate and identify Multiple Tropical Diseases
VereTheat to detect, differentiate and identify Anthrax, Small Pox, Plague and Tularemia
VereFoodborne to detect, differentiate and identify multiple foodborne Pathogens in one test
Verevet to detect, differentiate and identify multiple Avian Pathogens in one test
VereMERS to detect and identifies Middle East Respiratory Syndrome Coronavirus (MERS-CoV)
Spesifikasi VereFoodborneTM
Detects multiple foodborne pathogens
Multiple Probes (with duplicates) for Bacillus spp., Campylobacter jejuni / lari /
coli, Clostridium perfringens, Cronobacter sakazakii, Listeria spp., Salmonella
spp., Escherichia coli, Shigella spp., Staphylococcus aureus, Vibrio cholera, Vibrio
parahaemolyticus, stx1A gene, stx2A gene, etc
Process controls on each chip:
• PCR: Positive and Negative Controls
• Hybridization: Orientation and Hybridization Probes
Limit of Detection: 100 copies of extracted genomic DNA
Sample Types**: meat, fish, vegetables, milk, water, processed foods, stool or
vomit sample
Every chip is bar-code
Integrasi VereFoodborneTM
VerePLEXTM Biosystem menggabungkan biologi
molekuler, mikrofluida dan mikroelektronika untuk
membawa masa depan diagnostik. VerePLEXTM Biosystem
termasuk komponen-komponen berikut:
Temperature Control System (5 random access
modules)
Optical Reader
Biosystem Software
Barcode Reader
Feature & Cara Kerja Multiplex amplification reactions
Multiple probes per target ensures reliable detection of subtypes in every test
Small sample volume requirement
Fast and programmable temperature ramp rate
PCR yield is comparable to standard thermal cyclers
40% faster than conventional thermal cyclers
Proprietary microfluidic interface: contact surfaces are biocompatible and do not inhibit the PCR reaction
Short time required for fluidic operations
Technology & Manufaktur Chip silikon yang inovatif menggabungkan Micro-Electro Mechanical Systems
(MEMS) dengan mikrofluida yang memungkinkan integrasi multiplexing amplifikasi
nukleat asam dan deteksi dalam satu perangkat
The integrated silicon heaters, temperature sensors and miniaturized 25μl volume
allow the PCR module to perform the rapid heating and cooling cycles required for
highly reliable, end-point PCR.
The microfluidic integration of the PCR and hybridization modules ensures that
system operation does not require highly-trained personnel, and greatly simplifies
the typical lab procedures.
Diproduksi dengan kontrol room kelas 100 dan quality control 6 sigma metodology
dan termasuk on-the fly spotting with error detection
http://vereduslabs.com/technology/technologyverechip-technology/
6 sigma metodology (DMAIC)
Define adalah fase menentukan
masalah, menetapkan persyaratan-
persyaratan pelanggan, mengetahui
CTQ (Critical to Quality)
Measure adalah fase mengukur tingkat
kecacatan pelanggan (Y)
Analyze adalah fase menganalisis
faktor-faktor penyebab masalah/cacat
(X)
Improve adalah fase meningkatkan
proses (X) dan menghilangkan faktor-
faktor penyebab cacat
Control adalah fase mengontrol kinerja
proses (X) dan menjamin cacat tidak
muncul
Proses Fabrikasi
A variety of structures was made using this process flow: microchannels, mixers, microreactors, micropillar filters,
capillary pumps and valves, droplet generators (for digital droplet polymerase chain reaction, or PCR), mergers,
and splitters
a) A silicon wafer (grey color) is covered with an oxide layer (green color).
b) Then, a nitride layer (blue color) is added.
c) An etch is performed to remove nitride and oxide, and silicon is excavated to form the coarse structures.
d) The exposed silicon surface is oxidized. This oxide will serve as protection during the definition of fine structures.
e) The nitride is removed and the fine structures are etched
f) the oxide is removed
g) the silicon is bonded to Pyrex
h) A backside etch is performed to open fluidic connections.http://www.meddeviceonline.com/doc/silicon-a-material-with-huge-potential-for-lab-on-chips-0001
Contoh Teknologi dengan PCR
http://www.meddeviceonline.com/doc/silicon-a-material-with-huge-potential-for-lab-on-chips-0001
Pertama, sampel darah diambil
Kedua, pompa mendorong darah dan reagen ke
modul pra-amplifikasi. Modul ini terdiri dari mixer,
reaktor termal untuk PCR, dan filter.
Ketiga, dalam modul analisis, DNA dimurnikan
dicampur dengan reagen, allele-specific PCR
dilakukan sehingga hanya fragmen DNA dengan
SNP diperkuat, dan produk sampingan dari
amplifikasi terdeteksi dengan detektor
elektrokimia
Kelebihan
Memudahkan untuk mengontrol epidemi
pada makanan
Tes yang dipilih generik dan spesifik dari
patogen makanan yang di uji
Lab on Chip yang dirancang portable dan
dapat dioperasikan dengan mudah tanpa
membutuhkan tenaga ahli
Probe dapat di upgrade yang
memungkinkan dapat mendeteksi patogen
baru hasil dari mutasi
Perbandingan dengan Metode Konfensional (bacteriological Analytical Manual)
Terimakasih Banyak