VISCOSITY

Transport property defined as the retarding effect of slower layers of a fluid on an adjacent faster layer.

VISCOSITY

• Viscosity is a transport property that is observed when there is a momentum or velocity gradient.

• It is defined as the retarding effect of a slower layer of a fluid on an adjacent faster layer.

• Flux = Jx α -dv/dx where the proportionality constant = η = viscosity coefficient or constant ( g/(cm-s)

Experiment

1. Measure viscosity by measuring time (t) it takes a fluid to fall between two marks of viscometer. ηρ = Bt where ρ = fluid density and B = viscometer constant.

2. Determine B using water

3. Determine η of BSA as a function of its concentration

Experiment (2)

4. Determine effective spherical radius of BSA protein.

5. Repeat steps 3 and 4 for denatured BSA.

Two-Day Expt

• Day 1: Native BSA: make stock solution and its dilutions; measure η = f(c). Denatured BSA: make stock solution

• Day 2: d-BSA: make dilutions and measure η = f(c).