yuan-yun lin_isomrm
TRANSCRIPT
Yuan-Yun Lin , Ling Chao*, Kevin C.-W. Wu* Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan
Discussions
Objective
Acknowledgements
Characterization
SEM of MCNs
E-mail ：[email protected] and [email protected]
Synthesis of A Novel Phospholipid Layer/Mesoporous Carbon Nanoparticles (MCNs) Core/Shell Structure as An Anticancer Drug Nanovehicle
for Efficient Drug Loading
Synthesis of a Novel Phospholipid Layer/MCNs
Experimental
Doxorubicin
Hydrophobic Drug Loading
Lipid coating
MCNs
Vial DOPC@CCl4
Remove organic solvent under N2
Mix well
Add solution
Drug Loading Testing
centrifugation Optical Microscope Fluorescence Photometer
Analysis
TEM of MCNs
50 nm
BET of MCNs
Relative Pressure (p/p0)
Volu
me
Abs
orbe
d (c
m3 g
-1)
Pore Diameter (nm) V
P
0
50
100
150
200
250
300
350
0 0.2 0.4 0.6 0.8 1 1.2
0 0.05
0.1 0.15
0.2 0.25
0.3 0.35
0.4 0.45
0.5
0 50 100 150 200
Drug Loading
0
20
40
60
80
100
Load
ing(
%)
Results
Because of their high surface area and the hydrophobicity, mesoporous carbon nanoparticles (MCN) were successfully synthesized and utilized as a potential drug nanocarrier. In addition, a phospholipid layer was applied as a gate-keeper to control the load/release behavior of drugs. The MCN/phospholipid core/shell structure here provides a new alternative for next-generation bio-medical applications.
The research was supported by the National Taiwan University.
Synthesis of a Novel Phospholipid Layer/Mesoporous Carbon Nanoparticles for Intracellular Drug Delivery.
MCNs
Drug loading Lipids mixing
!
Cell Culture
(a) (b) drug
100 nm
5 µm
MCN+doxo.: 16.23 µmole/ doxo./g MCN MCN+doxo.+lipid: 8.76 µmole/ doxo./g MCN
MCN+doxo. MCN+doxo.+lipid
Texas Red @DHPE
MCN
fluorescence