Speaker : Shiue-Tai ChenAdviser ：鄒文雄 老師

結 構 生 物 學結 構 生 物 學Structural BiologyStructural Biology

期 中 報 告

PDB ID： 1HD2

Crystal Structure of Human Peroxiredoxin 5, a Novel Type of Mammalian

Peroxiredoxin at 1.5 Å Resolution

Jean-Paul Declercq,Christine Evrard,Andre Clippe et al. Journal of Molecular Biology 311(4): 751-759 2001

Reactive oxygen species (ROS) are constantly generated under normal conditions as a consequence of aerobic metabolism.

ROS include free radicals such as the superoxide anion (O2

.-), the hydroxyl radical (HO.), and th

e nonradical hydrogen peroxide (H2O2).

To cope with the deleterious effects of reactive oxygen species, aerobic organisms are equipped with antioxidant enzymes.

More than a decade ago, a novel family of peroxidases named peroxiredoxins (Prxs) emerged. (Chae,etc.1994)

Topological diagram showing the arrangement of the secondary structural elements in Peroxiredoxin 5

Ribbon diagrams showing the overall organization of Peroxiredoxin 5.

(b) being a top view of (a).

(a) Molecular surface coloured according to the local electrostatic potential,ranging from blue (the most positive region) to red (the most negative). (b) A diagram in which the side-chains of some important residues. (a) was produced using GRASP (b) using MOLSCRIPT26 and Raster3D.

View of the surrounding of the active-site pocket.

View of the surrounding of the active-site pocket.

(c) Stereoscopic view oriented like (b) in which the electron density of a s-A28 map has been contoured at a level of 2 s around the side-chains

and the benzoate ion. (c) was produced using using O.

What I will do?What I will do?

1. 使用 alignment in EBI and using GeneDoc in PSC

，為了比對 PeroxiredoxinⅠ~Ⅵ 胺基酸序列的特異性及保留性，與功能的關係。

2. 利用 Swiss pdb Viewer 軟體及網頁搜尋的PDB ID 檔，來觀察 PeroxiredoxinⅤ 與分子內或分子間的交互作用 (interaction) ，與酵素活性的關係。

What I will do?What I will do?

3. 使用上述的方法，來討論我們實驗室利用不同的物種 cloning 出來的 Peroxiredoxin 與分子生物學研究結果的相關性。

O2 O2-. SOD

H2O2

GPxH2O

ONOO-

NO.

NO2.

OH.

Fe2+

Fe3+

Catalase

PL

PLOOH

PLOH

Prx

Cu+

Cu2+

Prx

PHGPx

Zachary A. Wood,etc.2003

Zachary A. Wood,etc.2003

K. Henkle-Du¨hrsen *, A. Kampko¨ tter,2001

PDB IDPDB ID ：： 1HD21HD2