Protein PurificationProtein Purification

0800-009-695

/////12841 :0800-0 09-695Mail:Kimforest@gmail.com TEL:02-2790-2222 FAX:02-27929986

5

-- HiScaleHiScale ColumnColumnXK

Simple manual purification of antibodiesSimple manual purification of antibodiesrProteinrProtein A/G A/G GraviTrapGraviTrap TMTM

Cubee 2~1.5ml tube x 8 / 0.5ml tube x 8 / 0.2ml tube x 167200rpm

HisHis--tagged protein purificationtagged protein purification

Ni Sepharose

XK or Spin column 5 /25/100ml ~ 40 mg/ml 90 Ni Sepharose 6 FF 17-5318-06 /-01/-02

XK, Tricorn or Spin column 25 / 100 ml ~ 40 mg/ml 34 Ni Sepharose HP 17-5268-01/-02

Quantity Binding capacity Particalesize (um) Product Code number

Metal Chelate Affinity

XK or Spin column 50 ml ~ 40 mg/ml 90 Chelating Sepharose 6 FF 17-0575-01

XK or Spin column 25/100 ml ~ 40 mg/ml 90 IMAC Sepharose 6 FF 17-0921-07/-08

XK, Tricorn or Spin column 25/100 ml ~ 40 mg/ml 34 IMAC Sepharose HP 17-0920-06 /-07

Quantity Binding capacity Particalesize (um) Product Code number

Pre-packed Columns

Anti-His Antibody

5x1/5x5ml23 umol Cu2+/ml medium34HisTrap Chelating HP17-0408-01/-0409-03

5x1/5x5ml> 40/200 mg His-tagged protein34HisTrap HP17-5247-01/-5248-02

5x1/5x5ml> 40/200 mg His-tagged protein34HisTrap IMAC HP17-0920-03/-0920-05

5x1/5x5ml> 40/200 mg His-tagged protein90HisTrap IMAC FF17-0921-02/-0921-04

5x1/5x5ml~ 40/200 mg His-tagged protein90HisTrap FF crude11-0004-58/-5286-01

170ul (2.6ug/ul)Anti-His Antiboddy27-4710-01

5x1/5x5ml ~ 10 / 50 recombinant GST 90 HisTrap FF17-5319-01/-5255-01

20 ml 800 mg His-tagged protein90 HisPrep FF 16/10 28-9365-51

10x 1 ml ~ 40 mg His-tagged protein 90 His GraviTrap11-0033-99

50x100 ul~750g His-tagged protein 34 His SpinTrap28-4013-53

Quantity Binding capacity Particalesize (um)

Product Code number

MBPMBP--& Strep (& Strep (llll))--taggedtagged

Dextrin Sepharose / StrepTactin Sepharose

Dextrin Sepharose

XK, Tricorn or Spin column 10 ml ~ 6 mg/ml 34 StrepTactin Sepharose HP 28-9355-99

XK, Tricorn or Spin column 25 ml 7~16 mg/ml 34 Dextrin Sepharose HP 28-9355-97

Quantity Binding capacity

Particalesize (um)

Product Code number

Strep Tactin Sepharose

Pre-packed Columns

HiTap column5x1/5x5ml~6/30 mg Strep(II)-tagged protein34StrepTrap HP28-9075-46/-9075-48

HiTap column 5x1/5x5ml~10/50 mg Strep(II)-tagged protein34 MBPTrap HP 28-9187-78/-9187-80

Quantity Binding capacity Particalesize (um) Product Code number

>tagged-protein Transition metals : Ni2+, Cu2+, Zn2+,Co2+, Fe2+, Fe3+, Cd2+and Hg2+

1.2.3.4.5.: : His GraviTrap/: HisTrap

Strep(ll) His-TagDual-tagMBPfolding

Cu2+ ,Zn2+, Co2+,Ni2+

GSTGST--tagged protein Systemtagged protein System

Cleavage enzymes Cleavage enzymes

Factor Xa protease: Mr 28~30 kD

Thrombin protease: Mr 37 kD

PreScission Protease: Mr 46 kD

XK or Spin column 10 ml > 5 mg/ml 90 Glutathione Sepharose 4B 17-0756-01

XK or Spin column 25 / 100ml ~ 10 mg/ml 90 Glutathione Sepharose 4 FF 17-5132-01 /-02

XK, Tricorn or Spin column 25 /100 ml ~ 10 mg/ml 34 Glutathione Sepharose HP 17-5279-01 /-02

Quantity Binding capacity

Particalesize (um)

Product Code number

pGEX Vectors products

Glutathione Sepharose

GST- Pre-packed Columns

Glutathione Sepharose

Cleavage site

Detection Anti-GST Antibody

0.5ml ,50 dectectionsAnti-GST antibody27-4577-01

HiTrap column5x1/5x5ml~ 10 / 50 recombinant GST34GSTrap HP17-5281-01/-5282-02

75ulAnti-GST HRP ConjugateRPN1236

HiTrap column5x1/5x5ml ~ 10 / 50 recombinant GST 90 GSTrap 4B28-4017-45/-4017-48

HiTrap column5x1/5x5ml ~ 10 / 50 recombinant GST 90 GSTrap FF 17-5130-01/-5131-02

HiPrep column 20 ml 200 recombinant GST 90 GSTPrep FF16/10 28-9365-50

Spin column 50 columns 400g GST-tagged protein 90 GST SpinTrap purification Modules 28-9523-59

Quantity Binding capacity Particalesize (um)Product Code number

New ! HiScaleXKadaptor)

XK columnadaptor) Pressure limit -- XK 16, XK 26 5 bar

C columnPressure limit 1 bar (14.5 psi, 0.1 MPa)

RC 10/16 reservoir

AC10/16/26 adaptor

C 26/100 column

C 26/70 column

C 26/40 column

C 16/70 column

C 16/40 column

C 16/20 column

C 10/40 column

C 10/20 column

C 10/10 column

Product

18-8793-01

Packing equipment

18-8778-01/-8766-01

Flow adaptor

28-9889-53

18-8770-01

28-9889-50

18-8768-01

28-9889-48

18-8776-01

28-9889-46

18-8774-01

18-8773-01

Code number

RK 16/26 reservoir

AK 16/26 adaptor

XK 50/30 column

XK 26/100 column

XK 26/70 column

XK 26/40 column

XK 26/20 column

XK 16/100 column

XK 16/70 column

XK 16/40 column

XK 16/20 column

Product

28-9645-03

Pack connector

28-9663-83/-84/-85

Flow adaptor

28-9644-44

28-9644-45

28-9645-13

28-9645-14

28-9644-24

28-9644-41

Code number

HiScale 16/26/50 adaptor

HiScale 50/40

HiScale 50/20

HiScale 26/40

HiScale 26/20

HiScale 16/40

HiScale 16/20

Product

19-5102-01

19-5103-01

19-5201-01

19-5202-01

19-5007-01 /-5110-01

Packing reservoir

19-5006-01 /-5109-01 /-5207-01

Flow adaptor

19-5103-01

19-5101-01

19-5003-01

19-5002-01

19-5001-01

Code number

10 LabMate PD-10 buffer reservoir 18-3216-03

50 Empty PD-10 columns 17-0435-01

Quantity Product Code number

Quantity Product Code number

25 ugpGEX-6P-1 28-9546-48

500 units PreScission Protease27-0843-01

25 ugpGEX-4T-1 28-9545-49

500 unitsThrombin protease27-0846-01

25 ugpGEX-5X-1 28-9545-53

400 unitsFactor Xa Protease27-0849-01

PD-10 & reservoir

XK column

HiScale column

Empty ColumnsEmpty Columns

Antibody purificationAntibody purificationSpecificity Guide

HiTrap columns

XK or Spin column 5/25 ml > 20 mg/ml 90 Protein G Sepharose 4 FF (for IP) 17-0618-01/-02

XK or Spin column 5/25 ml > 35 mg/ml 90 rmp Protein A Sepharose FF 17-5138-01/-02

XK or Spin column 25 ml ~ 20 mg/ml 90 Protein A Sepharose CL-4B 17-0963-03

XK or Spin column 1.5 g ~ 20 mg/ml 90 Protein A Sepharose CL-4B 17-0780-01

XK or Spin column 5 /25ml ~ 6 mg/ml 90 nProtein A Sepharose 4 FF (for IP) 17-5280-01/-04

XK or Spin column 5 /25ml ~ 6 mg/ml 90 rProtein A Sepharose FF 17-1279-01/-02

Spin column 2 x 2 ml Immunoprecipitation Starter Pack (for IP) 17-6002-35

Quantity Binding capacity Particalesize (um) Product Code number

Pre-packed Columns

GraviTrap coiumn10x1 ml~ 50 mg human IgG90rProtein A GravitTrap28-9852-54

GraviTrap coiumn10x1 ml~ 20 mg human IgG90Protein G GraviTrap28-9852-55

Spin column16 x 100l>2.5 mg human igG34Protein G HP SpinTrap28-9031-34

Spin column16 x 100l ~2 mg human igG34ProteinA HP SpinTrap28-9031-32

HiTrap column5x1/5x5ml25/125 mg human IgG34HiTrap Protein G HP17-0404-01/-0405-03

HiTrap column5x1ml5 mg human IgM34HiTrap IgM Punification HP17-5110-01

HiTrap column1x5ml100 mg IgY34HiTrap IgY Punification HP17-5111-01

HiTrap column5x1/5x5ml 20/100 mg human IgG34 HiTrap Protein A HP 17-0402-01/-0403-03

HiTrap column5x1/5x5ml50/250 mg human IgG90 HiTrap rProtein A FF 17-5079-01/-5080-02

Spin column 50x100l>1 mg IgG/ column 34 Ab SpinTrap28-4083-47

Quantity Binding capacity

Particalesize (um)

Product Code number

Protein A / Protein G Sepharose

IP

Hot

Hot

Mag SepharoseTM Xtra--

His buffer kit11-0034-00

Mag Rack 6 (28-9489-64

TiO2 Mag Sepharose28-9440-10/28-9513-771x0.5 /4x0.5 ml

NHS Mag Sepharose28-9440-09/28-9513-80

10x1 mlHis Mag Sepharose Ni28-9799-17

2x1 /5x1ml~ 50 mg histidine-tagged protein /ml

His Mag Sepharose Ni28-9673-88/-90

2x1 /5x1ml> 27 mg human IgG/ml gelProtein G Mag Sepharose Xtra28-9670-66/-70

2x1 /5x1ml > 27 mg human IgG/ml gelProtein A Mag Sepharose Xtra28-9670-56/-62

Quantity Binding capacity Product Code number

Capacity target protein non-adherentsmearingcapture target protein (g ~ mg)

Hot

IP

FPLC

HisTrapHisTrap ColumnColumn

NEWNEW

Affinity purificationAffinity purification

Coupling gel

XK or Spin column 15 g 90 Activated Thiol Sepharose 4B 17-0640-01

XK or Spin column 15 g 90 Activated CH Sepharose 4B 17-0490-01

XK or Spin column 15 g 90 CNBr-activated Sepharose 4B 17-0430-01

XK or Spin column 10 g 90 CNBr-activated Sepharose 4 FF 17-0981-01

XK or Spin column 25 ml 90 NHS-activated Sepharose 4 FF 17-0906-01

Quantity Particalesize (um)

Product Code number

spacer arm

Ligand spacer arm - (ligand) (Mr < 5,000) spacer arm

Group specific gel

NA

NA

6 mg/ml

>18 mg/ml

NA

NA

>35 mg/ml

Binding capacity

XK or Spin column 5/100ml90Con A Sepharose 4B17-0440-03/-01

XK or Spin column 25 ml90Lentil Lectin Sepharose 4B17-0444-01

XK or Spin column 50 ml90Blue Sepharose 6 FF17-0948-01

XK or Spin column Tricorn5 ml34Stretavidin Sepharose HP17-5113-01

XK or Spin column 10 ml90 IgG Sepharose 6 FF17-0969-01

XK or Spin column 10 ml 90 Calmodulin Sepharose 4B17-0529-01

XK or Spin column 25 ml 90 Benzamidine Sepharose 4 FF (high sub)

17-5123-10

Quantity Particalesize (um)

Product Code number

Pre-packed Columns

HiTrap column5x1 ml>300nmol biotin34HiTrap Streptavidin HP17-5112-01

Spin column16 x 100l >0.6 mg biotinylated BSA34Streptavidin HP SpinTrap28-9031-30

HiTrap column5x1/1x5mlApplication dependent34HiTrap NHS-activated HP17-0716-01/-0717-01

HiTrap column5x1/1x5ml20/>100mg HSA34HiTrap Blue HP17-0412-01/-0413-01

HiTrap column5x1/1x5ml>35/175 mg trypsin90HiTrap Benzamidine FF17-5143-01/-5144-01

HiTrap column5x1/5x5ml 3 / 15 mg antithrombin III34 HiTrap Heparin HP17-0406-01/-0407-03

HiPrep20 ml 40 mg antithrombin III90HiPrep 16/10 Heparin FF17-5189-01

Spin column2.5 mlFor phosphorylated peptide90 Phos SpinTrap Fe28-9298-81

Spin column 5 ml34 NHS HP SpinTrap28-9031-28

Quantity Binding capacity

Particalesize (um)

Product Code number

DesaltingDesalting Easy, small batch purification

Pre-packed Columns

Hot

Spin column50 x 500l 70~130ul85~260PD SpinTrap G-2528-9180-04

HiPrep column1x53/4x53ml

Ion Exchange protein purificationIon Exchange protein purification

Sepharose

Pre-packed Columns

HiPrep column20 ml2,200 mg HSA90HiPrep 16/10 DEAE FF17-5090-01

HiPrep column20 ml860 mg BSA90HiPrep 16/10 ANX FF(high sub)17-5191-01

HiPrep column20 ml2,400 mg HAS90HiPrep 16/10 Q FF17-5190-01

Tricorn column1 ml< 50 mg10Mono S 5/50 GL17-5168-01

RESOURCE column1 ml< 25 mg15RESOURCE S 1ml17-1178-01

RESOURCE column1 ml < 25 mg15 RESOURCE Q 1ml17-1177-01

HiLoad column20 ml 160 mg/ml 90 SP Sepharose XL 17-5073-01

XK column 300 ml >130 mg/ml 90 Q Sepharose XL 17-5072-01

XK column 25/500 ml 5 mg/ml 90 ANX Sepharose 4 FF (high sub) 17-1287-10 /-01

XK column 25/500 ml 50 mg/ml 90 CM Sepharose FF 17-0719-10/-01

XK column 25/300 ml 70 mg/ml 90 SP Sepharose FF 17-0729-10/-01

XK column 25/500 ml 110 mg/ml 90 DEAE Sepharose FF 17-0709-10/-01

XK column 25/300 ml 120 mg/ml 90 Q Sepharose FF 17-0510-10/-01

XK or Tricorn column 25/100 ml NA 75 Capto adhere NEW 17-5444-10/-01

XK or Tricorn column 25/100 ml >45 mg/ml 75 Capto MMC 17-5317-10/-02

XK or Tricorn column 25/100 ml >100 mg/ml 90 Capto ViralQ28-9032-30/-31

XK or Tricorn column 25/100 ml >120 mg/ml 90 Capto S 17-5441-10/-01

XK or Tricorn column 25/100 ml >90 mg/ml 90 Capto DEAE NEW 17-5443-10/-01

XK or Tricorn column 25/100 ml >100 mg/ml 90 Capto Q 17-5316-10/-02

XK or Tricorn column 50 ml NA 30 SOURCE 30S 17-1273-01

Tricorn column 10/50 ml NA 15 SOURCE 15S 17-0944-10/-01

XK or Tricorn column 50 ml NA 30 SOURCE 30Q 17-1275-01

Tricorn column 10/50 ml NA 15 SOURCE 15Q 17-0947-20/-01

Quantity Binding capacity Particalesize (um) Product Code number

Hot

Hydrophobic interaction protein purificationHydrophobic interaction protein purification

Pre-packed Columns

XK column 25/200 ml 27 mg/ml 90 Octyl Sepharose 4 FF 17-0946-10/-02

XK column 25/100 ml 7 mg/ml 90 Butyl Sepharose 4 FF 17-0980-10/-01

XK column 25/200 ml NA 90 Butyl-S Sepharose 6 FF 17-0978-10/-02

XK column 25/200 ml 36 mg/ml 90 Phenyl Sepharose 6 FF (high sub) 17-0973-10/-05

XK column 25/200 ml 24 mg/ml 90 Phenyl Sepharose 6 FF (low sub) 17-0965-10/-05

XK or Tricorn column 25/200 ml NA 34 Butyl Sepharose HP 17-5432-01/-02

XK or Tricorn column 75 ml 20 mg/ml 34 Phenyl Sepharose HP 17-1082-01

Quantity Binding capacity Particalesize (um) Product

Code number

Hydrophobicity : Butyl-S < Butyl < Octyl < Phenyl

HiTrap column5x1/1x5ml>90/450 mg ovalbumin90HiTrap Capto DEAE28-9165-37/-40

HiTrap column5x1/1x5ml75HiTrap Capto adhere 28-4058-44/-46

HiTrap column5x1/1x5ml>45/225 mg BSA at 30 mS/cm75HiTrap Capto MMC11-0032-73/-75

HiTrap column5x1/1x5ml>500 mg BSA90HiTrap Capto viralQ28-9078-09

HiTrap column5x1/1x5ml>120/600 mg lysozyme90HiTrap Capto S 11-5441-22/-23

HiTrap column5x1/1x5ml70/350 mg HSA34HiTrap Q HP17-1153-01/-1154-01

HiTrap column5x1/1x5ml55/275 mg ribonuclease A34HiTrap SP HP17-1151-01/-1152-01

HiTrap column5 x1 mlDepends on medium75 / 90HiTrap Capto IEX selection kit28-9343-88

HiTrap column5x1/1x5ml>100/500 mg BSA90HiTrap Capto Q 11-0013-02/-03

HiTrap column5x1/1x5ml160/900 mg lysozyme90HiTrap SP XL17-5160-01/-5161-01

HiTrap column5x1/1x5ml120/600 mg HSA90HiTrap Q FF17-5053-01/-5156-01

HiTrap column5x1/1x5ml130 /165 mg BSA90HiTrap Q XL17-5158-01/-5159-01

HiTrap column5x1/1x5ml50/250mg ribonuclease A90HiTrap CM FF17-5056-01/-5155-01

HiTrap column5x1/1x5ml43/215 mg BSA90HiTrap ANX FF(high sub)17-5162-01/-5163-01

HiTrap column5x1/1x5ml120/600 mg HSA90HiTrap DEAE FF17-5055-01/-5154-01

HiTrap column5x1/1x5ml70/350mg ribonuclease A90HiTrap SP FF17-5054-01/-5157-01

HiTrap column7 x1 mlDepends on medium34 or 90HiTrap IEX selection kit17-6002-33

Quantity Binding capacity

Particalesize (um)

Product Code number

Pre-packed Columns (Ion Exchange continue)

Sepharose

HiTrap column5x1/1x5mlLigand density:40umol/ml34HiTrap Phenyl HP17-1351-01/-5195-01

HiTrap column5x1/1x5mlLigand density:40umol/ml34HiTrap Butyl HP28-4110-01/-05

HiTrap column5x1/1x5mlLigand density:40umol/ml90HiTrap Octyl FF17-1359-01/-5196-01

RESOURCE column1 ml > 25 mg15 RESOURCE PHE17-1186-01

HiTrap column5x1/1x5mlLigand density:40umol/ml90HiTrap Butyl-S FF17-0978-13/-14

HiTrap column5x1/1x5mlLigand density:25umol/ml90HiTrap Phenyl FF (low sub)17-1353-01/-5194-01

HiTrap column5x1/1x5mlLigand density:40umol/ml90HiTrap Butyl FF17-1357-01/5197-01

HiTrap column5x1/1x5mlLigand density:40umol/ml90HiTrap Phenyl FF (high sub)17-1355-01/-5193-01

HiTrap column7 x1 mlDepends on medium34 or 90HiTrap HIC selection kit28-4110-07

RESOURCE column1 ml > 25 mg15 RESOURCE ETH17-1184-01

Quantity Binding capacity

Particalesize (um)

Product Code number

Capture

IntermediatePurification

Polishing

Res

olut

ion

Gel filtrationGel filtration Sepharose

Pre-packed Columns

HiLoad240 ml