ch4 indappenz
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8/9/2019 Ch4 IndAppEnz
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Industrial Applications
of Enzymes
Carbohydrates
Essential components of all livingorganisms and most abundant.
Chemical & Energy Balance in theBiosphere
Photosynthesis: Conversion ofatmospheric CO2 into carbohydrate
Respiration: Carbohydrates areingested by higher animals to yieldenergy and CO2 is released
nCO nH O nO CH O)2 2 2 2 n+ ⇔ + (
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Monosaccharides
(CH2O)n
Derivatives of
Polyhydroxy (-OH) aldehyde (-CHO) – Aldose
polyhydroxy (-OH) ketone (=CO)
– Ketose
n = 3 ~ 8 triose
tetrose
pentose
hexos
heptose
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Asymmetric carbon (AC):
Carbon with 4 different substituents
Number of stereo isomer = 2AC
Aldohexos has 4 AC.
It has 16 possible isometric forms. 8 L form: OH on the left side of
the highest AC
8 D form: OH on the right side
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Glucose
Glucopyranose
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Haworth Projection
α-D-glucopyranose
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Fructose
CH OH2
C=O
HOCH
HCOH
CH OH2
b
b
-D-fructose
( -D-fructofuranose)
HCOH
D-fructose
OHOH C2
CH OH2
OH
H
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Disaccharides
OCH OH2
OHOH C2
CH OH2
OH
O
Sucrose
OCH OH2
OCH OH2
OH
O
Lactose ( form)b
OCH OH2
OCH OH2
OH
O
Cellobiose ( form)b
OCH OH2
OCH OH2
OHO
Maltose ( form)a
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Amylose
Amylopectin
Cellulose
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Starch Hydrolysis Corn (or Glucose) Syrup
Ethanol Fermentation
High Fructose Corn Syrup – 42% fructose (Similar sweetness as
sugar)
Fructose
– 2.3 times sweeter than glucose
– 1.4 times sweeter than sugar
UsageSoft drinks, Canned fruits, Juice, Icecream, etc
Source:
Corn
– Low costs
– Useful by-products: oil, gluten, cornmeal, dry starch, fiber, etc.
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Corn Refining Process
α− amylase
StarchSlurry
Liquefactiontank
80 - 150 °CpH 6 - 73 hrs.
Saccharifi-cation tank
55 - 60 °CpH 4 - 4.5
40 to 80 hrs.
Flash Cooler
Filtration
Carbon Refining
Ion Exchanger
Evaporator
Isomerizationwith Immobilized
Glucose Isomerase
60 - 65 °CpH 7 - 8.530 mins. Filtration
Carbon Refining
Ion Exchanger
Evaporator
Cooler
HFCS42% Fructose
Glucose Syrup
Fungal Glucoamylase
15% D.E.
95%D.E.
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Cellulose Hydrolysis
Cellulose:
Most abundant in nature Typical Dry Wood
Cellulose 41%
Hemicellulose 26%
– Lower MW than cellulose – Contains pentoses
Lignin 28%
– Cementing stiffening polymer
– Polymers of phenolic compoundsOther 5%
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Structure of Cellulose Fibers
Cellulose Moleculres
Elementaryfibrils
Microfibrils
Cell wall
componentof wood
Elementaryfibril
Polyoses
Lignin
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Economic Bottlenects to
Biomass Utilization
Availability in large quantity
High costs of pretreatment
Total biomass utilization
Low solid reaction High cost of separation
Economic Bottlenects forEnzyme Hydrolysis
Slow reaction rate
High costs of enzymes High costs of pretreatment
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Attrition Bioreactor (ABR)
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0
1 0 0
2 0 0
3 0 0
4 0 0
5 0 0
6 0 0
7 0 0
0 1 2 3 4 5 6
T i m e ( h o u r s )
R e d u c i n g
S u g a r ( m g / g D M )
A B R w i t h 0 . 4 7 6 c m S .S . b a l l s
S T R w i t h 1 w k b a l l m i l l i n g
S T R
Hydrolysis of Newsprint in ABR
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0
0 .2
0 .4
0 .6
0 .8
1
1 .2
0 4 8 12 1 6 2 0Tim e (hours)
R e l a t i v e E n z y m e A c t i v i t y
Se a led Reactor
O p e n R e a c to r
Enzyme Stability in ABR
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