molecular mechanisms of yield & abiotic stress tolerance in rice joint laboratory for plant cell...

Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice

Joint Laboratory for Plant Cell Biology & Rice Genetics 

Professor LIN Hong-Xuan (SIPPE, Chinese Academy of Science)

Professor JIANG Liwen (The Chinese University of Hong Kong) 

中科院—裘槎基金联合实验室资助计划项目总结会

Review Meeting of the CAS-Croucher Funding Scheme for Joint Laboratories

Kunming (18th October 2012)

• Rice Genetics

• Rice breeding

• Rice Molecular biology

Prof. Lin (Rice Genetics)

Prof. Lin (Rice Genetics)

• Plant Cell Biology

• Protein trafficking and localization

• Organelle biogenesis and function

Prof. Jiang (Cell Biology)

Prof. Jiang (Plant Cell Biology)

• Genetics vs. Cell Biology

• Excellent track records in respective research areas

• Have on-going collaborative research

• Complement and syngenism 

The two PIs

Our goals and research plan• To understand the mechanisms of yield traits and stress tolerance in rice

• Potential application

• Important biological questions on rice biology and biotechnology

• Training of students and researchers

Yield traits of Rice

Molecular mechanisms of yield traits

Molecular breeding for high-yield crops

Molecular breeding for high-yield crops

Functional analysis of yield trait genes/QTLsFunctional analysis of yield trait genes/QTLs

Cell biological analysis of yield trait genes/QTLs

Cell biological analysis of yield trait genes/QTLs

Mapping and cloning of genes/QTLs related to yield traits

Mapping and cloning of genes/QTLs related to yield traits

NIL(GW2)                 S-7                     S-65                    S-85

FAZ1 NIL(GW2) FAZ1 NIL(GL3.1)

Research Plan

 Stress tolerance traits of Rice

Molecular mechanisms of stress tolerance

Molecular breeding for crops with improved tolerance

Molecular breeding for crops with improved tolerance

Functional analysis of stress tolerance genes/QTLs

Functional analysis of stress tolerance genes/QTLs

Cell biological analysis of stress tolerance genes/QTLs

Cell biological analysis of stress tolerance genes/QTLs

Mapping and cloning of genes/QTLs related to stress tolerance

Mapping and cloning of genes/QTLs related to stress tolerance

Research Plan

Progress of on-going collaboration

Mapping based cloning and functional analysis of grain length QTL GL3.1

GL3.1

Cloning of GL3.1

Overexpress GL3.1-WY3 increase the grain length

GL3.1 is predicted to encode a Ser/Thr

phosphatase with kelch domain

GL3.1 increase the grain length, weight and yield

The plot grain yield was significantly increased in NIL (+11.1%)

Subcellular location of GL3.1

GL3.1 in both parents was detected throughout the entire cell

Subcellular location of Cyclin-T1;3

When GL3.1 was co-expressed with Cyclin-T1;3, increased accumulation of GL3.1 was observed in the nuclei

• The Croucher Foundation• The CAS-Croucher Funding Scheme for Joint

Laboratories• CAS/SIPPE and CUHK