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Vi lng bit n su sc em xin chn thnh cm n PGS.TS Nguyn

Vn !"u #$ gi%& #' ti( t"n t)nh h*ng +,n - t& #i'u /i0n thu"n l1i gi23 em

h&n thnh t4t /h&5 lu"n t4t nghi03 ny.

6m xin chn thnh cm n 7%n ch8 nhi0m /h&% 9&5 h:c( c5c th;y c5c

c< t=&ng b> m mng h*ng tH ht nhn( 3hng 3hF hI3 thJ

nguyKn tL thu>c Vi0n h&5 h:cBT=ung tm /h&% h&5 tD nhiKn - c

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MC LCTrang

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PHN I. TNG QUAN

. Ah5i Ru5t -' h: Sim Uy=t%ce%e

. TFng Ru%n -' chi Eleist&c%lyx

-.- #l2i3toc%ly4 ci&cumci33%

-.. #l2i3toc%ly4 nig&%n3

-.5 #l2i3toc%ly4 n2&6o3um -.7 #l2i3toc%ly4 &2hn2&6inu3 W

-.8 #l2i3toc%ly4 con3p2&ipu%ct%tu3 W

. TFng Ru%n -' cy -4i XEleist&c%lyx &3e=cul%tus Y&xbZ [

.. !\c #im thDc -"t [

.. Phn b4 [

.. ]ng +Jng c8% cy -4i [..W Thnh 3h;n h&5 h:c ^

..W. Thnh 3h;n h&5 h:c c8% tinh +;u nJ h&% -4i ^

..W. Thnh 3h;n h&5 h:c c8% nJ h&% -4i _

.W U>t s4 t=itec3en c` h&t tanh sinh h:c liKn Ru%n #n #4i t*1ng

nghiKn cu

.W. E5c t=itec3en /hung &le%n%n

.W. E5c h13 chIt /h5c [

.[ U>t s4 tanh chIt l%-&n&i+ liKn Ru%n #n #4i t*1ng nghiKn cu

c8% #' ti

.[. SD 3hn b4 l%-&n&i+ t=&ng thDc -"t

.[. Tanh chIt #Onh tanh c8% l%-&n&i+ ^

.[. T5c +Jng sinh h:c c8% l%-&n&i+ _

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PHN II. MC TIU V PHNG PHP NGHIN C!U [

. UJc tiKu [

. Ph*ng 3h53 nghiKn cu [

PHN III. CC "#T QU V TH$ LU%N

. NguyKn li0u thDc -"t

. Ehit c5c h&t chIt sinh h:c t=&ng l -4i ^

. ThL #Onh tanh c5c l%-&n&it t=&ng c5c c\n d

.W Phn l"3 c5c chIt t=&ng c\n E d

.W. Sc / l3 mfng /h& s5t c\n E d

.W. Phn l"3 c5c chIt tinh /hit t=&ng c\n E

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3.6.1 '"# t$%c ch"t '5.

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PHN IV. TH&C NGHI'M ^

W. U,u nguyKn li0u ^

W. Jng cJ - h&5 chIt ^

W. Thit bO nghiKn cu d

W.W NghiKn cu c5c chIt t=&ng l5 -4i d

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W.W. Ehit 3hn l3 c5c chIt t=&ng l5 -4i d

W.W. Phn ng #Onh tanh l%-&n&it

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W.W.[ Phn l"3 c5c chIt tH nh`m E[bjng sc / c>t nh%nh W

W.W. Phn l"3 c5c chIt tH nh`m Ebjng sc / c>t nh%nh WW

W.W.^ Phn l"3 c5c chIt tH nh`m E^bjng sc / c>t nh%nh W

PHN V. "#T LU%N Wd

TI LI'U THAM "H$ W

PH LC

M U

Vi0t N%m c` /ha h"u nhi0t #i km nKn h0 thDc -"t =It 3h&ng 3h2 -i

hn ___ l&i cy chi% lm [__ chi - __ h:. !y l ngun ti nguyKn

thiKn nhiKn Ru gi5( cung cI3 ngun nguyKn li0u ch& ngnh h*ng li0u( m

3hkm - h&5 +*1c. E5c nh nghiKn cu #$ tFng h13 #*1c =It nhi'u h13 chIt

c` t5c +Jng ch?% b0nh( s&ng c5c h13 chIt ny c` m>t s4 hn ch nhIt #Onh(

nh* l gy nh?ng 3hn ng 3hJ /h

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#*1c #\c bi0t Ru%n tm l nghiKn cu c5c h13 chIt #*1c t5ch =% tH sn 3hkm

thiKn nhiKn.

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T=ung ( #*1c nhn +n +Mng nIu n*c u4ng( /ach thach tiKu h&5. 5 -4i - nJ

-4i lm thu4c ch?% mJn nh:n( l l&ot( ghp. Ui #y -i0n !t( -iKm h:ng( b0nh ng&i +%. TH

nm t= li #y( m>t s4 ct s4 chItc` h&t tanh /h5ng 9rV. !\c bi0t m>t nghiKn cu g;n #y nhIt ch& thIy n*c

chit c8% nJ V4i l thnh 3h;n c8% thu4c t=1 tim. Nh* -"y( cMng -i sD thu"n

l1i -' nguyKn li0u th) #y l nh?ng l +& /hach l0 ch2ng t

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PHN I. TNG QUAN

(.( "h)* +,)- / h0 1*2 M34-a56a6

Ey -4i c` tKn /h&% h:c l #l2i3toc%ly4 op2&cul%tu3Y&xb( thu>c chi

#l2i3toc%ly4( h: Sim Uyt%ce%e. E5c cy thu>c h: Sim th*@ng m:c xen l,n

-i c5c cy g /h5c thnh #%i =Hng nhi0t #i( at /hi m:c thu;n l&i.

9: Sim 3hn b4 c5c -Mng =Hng nhi0t #i - nhi0t #i( ch8 yu

chu U - chu c.

9: Sim l m>t h: ln gm _ chi - ___ l&i( cy thu>c h: Sim c`

#\c #im thDc -"t nh* s%u v

Ey g ln( cy nhQ h&\c cy bJi. 5 #n( h&\c mo3 c` /ha% =ng( l5

th*@ng m:c #4i nh%u - c` tuyn mQ. 9&% m:c thnh cJm n5ch h%y #;u

cnh( c` h)nh xim h&\c h)nh chMm. 9&% #'u l*Qng tanh. !i h)nh 4ng +anh

h&n t&n -& b;u h%y chw +anh m>t at. 5 #i gm WB[ c5nh x3 l13 -& nh%u(

at c` 4ng +i nguyKn.

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thnh 4ng ngn v b%& 3hIn #anh l*ng h%y g4c. !% m"t /ht

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thuz t=&ng b;u th) n&$n x3 thnh h%i h%y nhi'u +$y. Cu n%ng h&\c thOt( c`

s1i - th*@ng #*1c b:c /an bi 4ng #i. T=&ng Ru th*@ng c` ht( ht c` n>i

nhq( 3h

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n3 c%& h&% c` W c5nh( c%& mm( c` nhi'u tiu nhJy. Ph) Ru t=n h%y +i( t&

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Phn b4 v =Hng - 7c T=ung N%m.

1.2.4 Cleistocalyx rehnervinus

TKn Vi0t N%m v V4i gn mng

!\c #im thDc -"t v Ey #i m>c nhf( nh5nh t=n x5m( t& Bmm. 5

c` 3hin b;u +Jc #n t=5i x&%n( t& BWx[.[B^cm ch`t l5 =>ng( #5y t=n gn

3hJ mOn( c5ch nh%u WBdmm( gn c5ch b)% Bmm. Ph5t h&% l5 - ng:n. 9&%nh* /ht nm c` mM% h&% n -& th5ng - gi?% th5ng ^.

Phn b4 v Th%nh 9&5( Ngh0 Tnh( 7)nh T=O ThiKn( Cung N%m( !

N~ng( Gi%%iBA&nTum.

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nh?ng #4m mu nu( cu4ng +i B.[cm. 9&% g;n nh* /h

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Nm __( !& ThO Th%nh 9i'n( t=*@ng !9 *1c 9 N>i - nh?ng

ng*@i /h5c #$ tin hnh thL nghi0m nghiKn cu m>t s4 t5c +Jng c8% cy -4i.

At Ru ch& thIy vB Tanh /h5ng /hukn c8% l5 -4i( #\c bi0t l l5 -4i 8 c` t5c +Jng =It t4t

t=Kn -i /hukn:coli( l l&i -i /hukn th*@ng gy =% b0nh #*@ng =u>t( - h%i -i

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u4ng n*c sc l5 -4i # ch?% b0nh tiKu chy - tm n*c sc l5 -4i # ch?%

b0nh -iKm +% c8% nhn +n.

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#;y bJng( /h` tiKu.

B At Ru thL t5c +Jng #>c t b& c8% m,u chit tH l5 -4i b*c #;u ch&

thIy c tinh +;u - c%& th< t&n 3h;n #'u c` /h nng c ch sD 3h5t t=in t

b& ung th* Xung th* g%n( ung th* mng tim( ung th* tL cungZ. !i'u ny m

=% h*ng nghiKm cu mi #4i -i +*1c li0u l5 -4i.

Nm d( Nguyn !c Uinh( 3hng #

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Thnh 3h;n h`% h:c c8% tinh +;u -4i #*1c chw =% t=&ng bng +*i

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l*1ng c8% c5c chIt /h5c nh%u( t=&ng tinh +;u l5 -4i th) at chIt hn Xd chItZ.

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:,;< axit oleanolic c= ho>t tnh 0h!n2 chn2 ?i 0h#@n B.

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1.4.2 Cc hp cht khc

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phn l&p t loi icania hete$o7o$pha< l>i thR hiSn 0h nFn2

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thIy c` l%-&n&it v m%=ylli+%ce%e( =%ce%e( E%nn%ce%e( E&mmelin%ce%e(

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c` nh`m &B+ihy+=&xy3hen&l.

^ PhRn n9 9hv g* 2,* *awEn*.E5c +,n chIt l%-&n&it c` nh`m 9 -O t=a ^ c` th 3hn ng -i mu4i

+i%&ni # t& thnh chIt mu %&ic -ng c%m #n #f.

1.5.3 T6c '(ng sinh h-c c)a lavonoit

E5c +,n chIt c8% l%-&n&it c` /h nng +"3 tt c5c g4c tD +& nh* 9(

Y. E5c g4c ny sinh =% t=&ng t b& bi nhi'u nguyKn nhn - /hi sinh =%

cnh N th) s gy =% nh?ng nh h*ng nguy hi nh* gy =% bin +O( h8y

h&i t b&( gy ung th*( tng nh%nh sD l$& h`%. Tha nghi0m ch& thIy /h

nng +"3 tt c8% m>t s4 l%-&n&it the& th tDv

Uy=icetin Rue=cetin m&=in +i&smetin n%=ingenin %3igenin

c%techin [(^ +ihy+=&xylB( W( [Bt=imeth&xy l%-&n =&binin /%em3e=&l

l%-&n.

l%-&n&it t& #*1c 3hc -i c5c i&n /im l&i m chanh c5c i&n /im l&i

ny l x2c t5c c8% nhi'u 3hn ng &xy h`%. E5c l%-&n&it c` ( [( ( WB

hy+=&xy c` /h nng liKn /t t4t -i c5c i&n /im l&i #` the& 3hc

&xych=&m&n ( &xic%=b&nyl h&\c ( WB&ct&B+i&xy3hen&l .

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o o7e o o7eo

7eo

oxych$o7on oxyca$Monyl o$thoioxyphenol

Thnh 3h;n c8% mng t b& c` c5c chIt li3i+ + bO 3e=&xyt h`%( t& =%

nh?ng sn 3hkm lm =4i l&n sD t=%& #Fi chIt cqng +,n #n sD h8y h&i t

b&. !*% c5c chIt ch4ng &xi h`% nh* l%-&n&it -& c th # b& -0 t b& th)

c` th ngn ngH% c5c nguy c nh* x -?% #>ng mch( t%i bin mch( l$& h`%(tFn th*ng +& bc x( th&5i h`% g%n.

l%-&n&it cMng -i %xit %sc&=bic th%m gi% t=&ng Ru5 t=)nh h&t #>ng c8%

enym &xi h`%B/hL. l%-&n&it cn c ch t5c #>ng c8% hy%lu=&ni+%. 6nym

ny lm tng tanh thkm c8% m%& mch. Ahi enym ny thH% th) gy hi0n

t*1ng xuIt huyt +*i +% m y h:c g:i l b0nh thiu -it%min P

XP%-it%min&seZ. E5c ch 3hkm ch% l%-&n&it chit tH c5c l&i Eit=us nh*Eem%l%-&ne( Ei=cul%=ine l%-&n&it tH l5 bc h X+i&sminZ nh*

%l&n( i&smil( l%-&n&it tH h&% he X=utinZ -i nhi'u bi0t +*1c /h5c

nh%u #$ chng minh t5c +Jng lm b'n thnh mch( lm gim tanh +n -

tanh thIm c8% m%& mch. T5c +Jng ny #*1c h13 lDc cMng -i %xit %sc&=bic.

l%-&n&it #*1c +Mng t=&ng c5c t=*@ng h13 =4i l&n chc nng tnh mch( tnh

mch suy yu( gi$n tnh mch( t=( chy m5u +& #\t -ng t=&ng 3hJ /h&%( c5cb0nh t=&ng nh$n /h&% nh* sung huyt /t mc( =4i l&n tu;n h&n -ng mc.

E5c +,n chIt %nth&cy%n&it c` t5c +Jng t5i t& t b& -ng mc - #$ #*1c

chng minh c` t5c +Jng tng thO lDc -& b%n #Km.

T5c +Jng ch4ng #>c cu% l%-&n&it th hi0n lm gim th*ng tFn g%n(

b& -0 #*1c chc nng g%n /hi m>t s4 chIt #>c #*1c #*% -& c th s2c -"t

^

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tha nghi0m XEElW(( benen( et%n&l( E9El( Ruinin( n&-%sen&lZ. *i t5c

+Jng c8% l%-&n&it ng*Qng %sc&=bic #*1c Fn #Onh #ng th@i l*1ng glyc&gen

t=&ng g%n tng. SD tach lqy glyc&gen c` ngh% Ru%n t=:ng t=&ng -i0c nngc%& chc nng gii #>c g%n.

Vi0c sL +Jng m>t s4 +*1c li0u t=&ng #i'u t=O -iKm g%n( x g%n( b& -0

t b& g%n =It hi0u Ru nh* v cy %ctis&( c` bi0t +*1c l Eh&3hyt&l. Ey

CiliDum m%&i%numG%e=tn c` bi0t +*1c l Eh&3hyt&l. Ey CiliDum m%&i%num

G%e=tn c` bi0t +*1c eg%nl&n cy bJt +ImB!iDi3cu3 3%D@%&iEE%.

T5c +Jng /ach thach tit m"t th c5c chIt thu>c nh`m l%-%n&n(l%-&n( l%-&n&l - l%-%nBB&l.

l%-&n&it th hi0n t5c +Jng ch4ng c& tht nh?ng tF chc c nh~n Xt2i

m"t( 4ng +,n m"t( 3h Run - m>t s4 tF chc /h5cZ. Va +J( %3igenin c` t5c

+Jng lm gim c& tht 3h Run gy =% bi hist%min( %xetylch&lin( se=et&nin.

T=Kn b> m5y tit ni0u( nhi'u l%-&n&it thu>c nh`m l%-&n( l%-&n&l th

hi0n t5c +Jng thc c5c nh`m l%-&n(

l%-%n&n( +ihy+=&l%-&n&l( %nth&cy%nin( l%-%nBB&l( ch%le&n( is&l%-&n(

bil%-&n( WB%=ylc&um%=in( WB%=ylch=&m%n #'u #*1c chng minh bjng thDc

nghi0m +& c5c chIt l%-&n&it ny c ch c&n #*@ng sinh tFng h13

3=&st%gl%+in.

d

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Ng*@i t% sL +Jng =utin( cit=in( leuc&+el3hini+in( Rue=cetin( c%techin #

#i'u t=O b%n #f( -iKm +%( tFn th*ng +% - mng nh;y t=&ng t=*@ng h13 x t=O.

T=Kn h0 tim mch ( nhi'u l%-&n&it thu>c nh`m l%-&n&l( l%-%nBB&l(%nth&cy%nin nh* Rue=cetin( =utin( my=icetin( 3el%=g&nin( hn h13 c%techin c8%

t= c` t5c +Jng lm tng biKn #> c& b`3 - tng th tach 3h2t c8% tim( tha

nghi0m lm hi 3hJc tim /hi bO ng> #>c bi E9El( Ruinin( met%n&l( b)nh

th*@ng li sD =4i l&n nhO3.

E%& chit tH l5 cy bch Ru( *inko Diloh% ch% c5c +,n chIt B

=utin&si+ c8% /%em3e=&l( Rue=cetin - is&=h%mmetin Xt=&ng l5 gi #$ -ng th)ch% gin/getin - is&gin/getinZ #$ #*1c m>t s4 h$ng c8% Ph53 b& ch thnh

bi0t +*1c -a +J( Gin/&gin/( T%n%/%n c` t5c +Jng tng tu;n h&n m5u

t=&ng #>ng mch( tnh mch - m%& mch. Thu4c +Mng ch& nh?ng ng*@i c`

biu hi0n l$& suyv =4i l&n t=a nh( /h nng lm -i0c bjng t=a `c s2t /om( mIt

t"3 t=ung t* t*ng( h%y c5u gt.

T=Kn h0 th;n /inh( m>t s4 EBl%-&n glyc&it c8% ht t5&( HiGiphu3

6ulg%i3 -%=.s3in&sus Xch% s3in&sin( se=tisin - c5c +,n chIt %cyl c8%

s3in&sinZ c` t5c +Jng %n th;n = =0t.

U>t s4 ti li0u g;n #y c` n`i #n t5c +Jng ch4ng ung th* c8% m>t s4

chIt nh* leuc&cy%ni+in( leuc&3el%=g&ni+in( leuc&+el3hini+in - t5c +Jng

/h5ng 9rV c8% m>t s4 +,n chIt thu>c nh`m l%-&n nh* ch=ysin( %c%cetin ^BB

BBg%l%ct&3y=%n&si+.

E5c +,n chIt thu>c nh`m is&l%-&n&it c` t5c +Jng est=&gen -a +J

genistein X[( ^( W t=ihy+=&xyis&l%-&nZ +%iein X^( W +ihy+=&xyis&l%-&nZ.

T5c +Jng ny #*1c gii thach +& sD g;n nh%u -' cIu t=2c -i

+iethylstillb&est=&l.

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o

oho

ho

WaiTein

ho

oh

Wiethl tilMot$ol

U>t s4 l%-&n&it /h5c thu>c nh`m =&ten&i+ nh* chIt =&ten&n c` t=&ng

+y m"t(I2&&i3 2lliptic%7enth th) t5c +Jng +i0t c

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PHN 7. MC TIU V PHNG PHP NGHIN C!U

7.( M[5 -*K,

B y +Dng Rui t=)nh chit t5ch c5c l3 chIt t=&ng l5 -4i.

B Phn tach thnh 3h;n h&5 h:c c5c c\n chit bjng c5c 3h*ng 3h53

SAU.

B Phn t5ch c5c chIt tH c\n chit.

B 5c #Onh cIu t=2c 3hn tL c5c chIt 3hn l"3.

7.7 PhWn9 h) n9h*Kn 5,

B Ph*ng 3h53 chit c5c l3 chIt the& #> 3hn cDc c8% +ung mt # 3hn l"3 c5c chIt tH

c\n chit.

B Ph*ng 3h53 -"t l hi0n #i rY( V( US( 3hF c>ng h*ng tH ht nhn

X chi'u - chi'uZ # x5c #Onh cIu t=2c 3hn tL c5c chIt 3hn l"3.

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Phn 3. Kt qu v tho lun

3.1 X l mu v chitX l mu. ! ?Di ((leistocal$) opeculatusZoxM) Cc

th# h!i L Jmnh ph%c< ?o th!n2 , nF7 5. ! 0hn2 Md #

Cc $a >ch< $Vi ph`i 0h t$on2 M=n2 $7< a# C= "y L

nhiSt CU 0hon2 +55' cho Cn 0h 2ibn. Y2hifn nh l!

thnh MUt 7dn CR l7 n2#yn liS# chit.

'!c l]p ch"t c= 7t t$on2 l! ?Di 7 ch%n2 ti #an t7

l c!c kla?onoit ? c!c axit t$itecpencacMoxylic. Wo ?&y< 7Ut

#i t$Gnh x l 7# CH Cc xy n2 CR chit Cdnh h]n2

hai l]p ch"t ny.

Qui trnh chit. Ut l! ?Di (1i #n2 7i L 5' ]i !p #"t th"p

Cn 0hi cbn

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7>t l5 -4iST__ g%m

Och Ue9

Och 6tcOch E9ElVOch ete +;u h&

E\n 6 v ^(Wg9i0u suIt v S(TW

E\n E v d(dg9i0u suIt v SW(T

E\n v [_(dg9i0u suIt v d(T

Ngm VBW ngyt=&ng Ue9

Ehit bjngete +;u h& Ehit bjng

E9ElV

Ehit bjng6tc

m /h

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3.3 0nh tnh 2lvonoit v it trit4r/4n5cc6o"lic

! #Onh h*ng ch& -i0c 3hn l"3( ch2ng t

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[

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3.4.2 Phn tch b"ng s#c k! c)t

'!c thnh phn ca cn chit clo$oko7 CH Cc phn

t!ch t$n cUt t$#n2 !p< wn2 thit Md t!ch 9P' t$n ch"th"p phw ilica2el $a 2ii theo 2$aient< !p wn2 ?]i c!c hn

hp nh ete # haclo$oko7< nhexanaxeton< clo$oko7

7etanol.

Wa t$n 0t # 0ho !t /'< c!c phn Co>n $a 2ii

2iDn2 nha# Cc 2o7 l>i thnh c!c nh=7 phn Co>n< 0 hiS#

t '\ ',.

'!c phn Co>n 2Up l>i Cc phn t!ch tip Mun2 sc 0

cUt nhanh ? 0t hp ?]i 0t tinh phn Co>n< t 7Ut D

phn Co>n ch%n2 tA CH th# Cc 3 ch"t tinh 0hit^

(i) 'h"t C> nh&n Cc ]i >n2 c!c tinh thR hGnh 0i7

7# ?n2

t phn Co>n '5.1N

(ii) 'h"t C? nh&n Cc ]i >n2 c!c tinh thR hGnh 0i7

7# ?n2 t

phn Co>n '6.1.+ ?

(iii) 'h"t C@ nh&n Cc ]i >n2 c!c tinh thR hGnh 0i7

7# t$sn2

t phn Co>n '.1..

X phn t!ch c!c ch"t t cn clo$oko7 Cc 7inh hqa

nh L hGnh 3. ^

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E\n E

[(__ g%m

E&X^BZ

EXB[Z

EVXBZ

EWXVBZ

E[XV_BV[Z

EXV^BW^Z

E^XWB[Z

EdX_B^_Z

E[.BW

E[.BV

Tinh thNE[.

X[ mgZ

E..XBZ

E..X_BZ

E..V^B

E..W_BV

E^..B_

Tinh thNE..W

X[V mgZ

E^..XVBdZ

E^..VXBVWZ

E^..WW_B_

E..[W_B_

Tinh thNE^..X[ mgZ

Sc / c>t nh%nh =L% gii bjngete +;u h& XV_B_Z B c l&=&&=m XZ

Sc / c>t nh%nh =L% gii bjng%xet&n nBhecx%n XdZ

Sc / c>t nh%nh =L% gii bjngcl&=&&=m met%n&l X__Z

At tinh t=&ng met%n&lAt tinh t=&ng met%n&lAt tinh t=&ng /eten

^

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hiS# c# hai p$oton I? ICc t$n2 cho c!c chalcon cQn2

c= 7t t$on2 ph{ ny (,. pp7 ? .- pp7). Y2oi $anh. /$on2 ph{ 1IY9Z ca C>cbn c= tn hiS# ca 7Ut

nh=7 7etoxi ('I3) L 3.66 pp7 ? 7Ut nh=7 I nu7 2n

nh=7 '* (13.5, pp7). / phn tch L t$n cQn2 nh

CDi chi# ?]i c!c 2i! t$d ' t$on2 ph{ 13'Y9Z ? 0ho !t

ph{ I9r' ? I9' c"# t$%c ca ch"t C>CH Cc x!c Cdnh l

t s4 mnh chanh nh* s%uv

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Io

o

c

oh

ch3

h3c

o'I3

oh

ch3

o

h3c

oh

c

o

ch3

ch3o

ch3

oh

c

oh

o

ch*ch(

ch3

ohch3o

ch3

oh

c

o

ch

ch(

ch3

7BT1-+

7BT1.+

7BT(,3

7BT15

7BT((1

7BT))

W_

8/13/2019 cy v i

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m5*2s-x


Ph{ Y9Z ? 9X xe7 phn thc n2hiS7 ? Phw lwc :;

9Ut D hun2 D ?&t l ca C>Cc n# $a t$on2 Mn2 3.1

ChZ-

Hunhjn92,Dy5

Nh*Y-i

nn95hR3BzC

{,n92g*rl--*nh

1"LM H*Y,D,Z-h@ns

Bz|zz

}q {,n9 2g*Dy5rx

1y5 h8

Van**n

|HD1$`

c

q6C?

# S.[9)nh /imBmu -ng

s,m

[E El&=&&=m _.^W 6te +;uhf%

cl&=&&=m

XZ

Vngs,m

Nunht

.^

# .S.ng b>tBmu -ng

s,m

E 6te +;uhf%

%xet&n

_.^ 6te +;uhf%

cl&=&&=mXZ

%c%m

Nu _.^

# .S.^9)nh /imBmu -ng

s,m

_E Uet%n&l _.^ 6te +;uhf%

%xet&n

Tams,m

Ah

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Kho !t 0 h`n c!c ph{ I9r' ? I9' CH cho ph[p

0t l#&n c"# t$%c ca ch"t C? l hi$oxi57etoxi6

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9Ut D hun2 D ?&t ca C?Cc n# $a t$on2 Mn2 3.1

3.*.3 Cu tr+c cht C-.

Ph{ 1I ? 13'Y9Z cho th"y ch"t C@ c= +, p$oton ? 3

n2#yn t cacMon. Ph{ 0hDi ln2 (EA9X) ch $a pic ion phn

t 7BT +56 (9j) ? c!c 7nh phn $H L +,< 3< 1,- t`n2

4n2 ?]i phn $H eto *iels+,ldet 7Ut t$itecpen th#Uc Hy

#$1en ?]i nh=7 I L ?bn2 O. Yh ?&y< cn2 th4c phn t

ca C@ l '3I+,3.

'"# t$%c ca CH Cc x!c Cdnh a ?o c!c tn hiS# x#"t

hiSn t$n c!c ph{ Y9Z. Ph{ WEP/ ca C@cho th"y t$on2 3

n2#yn t cacMon< c= n2#yn t cacMon M&c MDn< 5 nh=7

'I< 1 nh=7 'I? nh=7 'I3. J Cif# ny ph} hp ?]i

nh&n x[t $un2 ch"t C@l 7Ut hp ch"t t$itecpen nF7 ?bn2.

Kt hp phn tch c!c tn hiS# t$n c!c ph{ 1I ? 13'Y9Zt Silic%gen #$ tkm chIt -& c>t( =c l3 mfng Silic%gen lKn

t=Kn # b& -0.

Tin hnh =L% gii bjng h0 +ung m 3hn cDc tng +;nv ete

+;u h& B E9Elv v^ vd v __ E9Elv E9El B Ue9 v dv

^v. At Ru thu #*1c ^_ 3hn #&n.

E5c 3hn #&n thu #*1c #em tin hnh SAU -i h0 +ung m3 c5c 3hn #&n c` sc # gi4ng nh%u thnh m>t nh`m

#5nh s4 tH E_ Ed. EIt l&i +ung m

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`.`.c Ph@n s 5)5 5hZ- -~ nh2 Ccn9 1" 5i- nhanh

Nh`m E[ l m>t hn h13 chIt =n c` /h4i l*1ng l [_mg( mu #en

s,m. 9n h13 ny ti3 tJc #*1c SAU -i nhi'u h0 +ung m

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PhF rY XA7=Z v W(d (_ ^_ W[(_ [d(^ _([d

XcmBZ.

PhF US v ch& c5c mnh m v d d W _W ^^.

PhF 9BNUY v ch& bit c` d9 t=&ng 3hn tL.

PhF 6PTB_ v ch& bit c` ^ nh`m E9.

PhF 6PTB[ v ch& bit c` nh`m E9( /h

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Tin hnh sc /a c>t nh%nh nh`m E. 90 +ung m

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Nh`m E^.s%u /hi /t tinh li nhi'u l;n t=&ng met%n&l thu #*1c chIt

tinh /hit( c` /h4i l*1ng [mg. T=Kn bn mfng c` Y _(^ -i h0 +ung m chuyn

+Och h`% h:c X33mZ nh* s%u v

d( W( ( ^(_ [[( W^(^ W([[ WW(

W(W W(_[ ( d(^d d(WW ^( (dW (_d (

(W^ _(^_ d( ^(^ ^( [([ ([ (W (^

d( ^( [([ [(

[

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PHN V. "#T LU%N

[^

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TI LI'U THAM "H$

. V Vn Ehi

TH #in cy thu4c Vi0t N%m.N7 h:c( T= _.

. TH #in 75ch /h&% *1c h:c.

N7 tH #in 75ch /h&%( 9 N>i( ( T=^[.

. GS. TS ! TIt 1i.Nh?ng cy thu4c - -O thu4c Vi0t N%m.

N7 h:c( ( T= W.

W. *ng y K T=;n !c.

Ey thu4c Vi0t N%m.

N7 NN 9 N>i( ^( T= [WB[[.

[. V Vn Ehi( Vq Vn EhuyKn - c5c c>ng sD.

Ey cf th*@ng thIy Vi0t N%m XrrZ.

N7 A9 - AT 9 N>i( ^( T= WWBW^.

. Phm 9&ng 9>Ey cf Vi0t N%m XrrZ.

N7 T=p( W.

^. Nguyn !c Uinh.

Tanh /h5ng /hukn c8% cy thu4c Vi0t N%m.

N7 h:c( ^.

[d

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d. Nguyn Tach( T=;n 913.

TKn cy =Hng Vi0t N%m.

N7 N

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