cory ni bacteria
DESCRIPTION
بكتريااااااااااااااااTRANSCRIPT
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Genus:corynebacteria
Members of the genus corynebacteria
GENERAL CHARACTER:
1-Morphology:
-G+ve pleomorphic bacili with club shaped swelling at
one or both ends.
-arranged in chinese letters
(coryneform morphology)include coccoid ,rod,club and filamentous shape.
-Non(spore forming,motile,capsulated).
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2-culturally:-
-facultative anaerobic
(37c and PH7.2-7.4)
-grow on blood agar producing hemolysis
3-Biochemically:-
-catalase positive
(except Arcanobacterium pyogenes)
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Most common species:-
c.Pseudotuberculosis
(c.ovis)
c.renale
c.Pyogenes
(Arcanobacterium pyogenes)
c.equi
(Rhodococcus equi)
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Pathology of corynebacteria:-
Species HostDiseasec.Pseudotuberculosis c.ovisSheep and goat(ovine biotype)Caseous lymphadenitisHorses(equine biotype) ulcerative lymphangitsCattle and buffaloOedematous skin diseasec.Renale groupcattlecystitisSheep and goatUlcerative balanoposthitisArcanobacterium pyogenes(c.pyogenes)cattle-suppurative infections in different tissues-summer mastitisRhodococcus equi (c.equi)Foals and horses-suppurative pneumonia and pulmonary abscessation) -
Pathogenicity of corynebacteria:-
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c.Pseudotuberculosis
1-Morphology:-
-G+ve bacili.
-chinese letters.
-Non motile.
-Non spore forming.
-Non capsulated.
2-culture characteristics:
-Facultative anaerobic
(with optimum temp is 37c and PH is 7.2-7.5)
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**Media for isolation**
-Blood agar beta hemolysis
-coloies are small whitish.
3-Biochemical reactions:-
-catalase positive.
-Ferment glucose and maltose.
-Have 2 types:
Ovine biotype is (nitrate reduction-negative)
Infect ovine,caprine
Equine biotype is(nitrate reduction-positive)
Infect equine,bovine
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Virulence factors:
1-cell wall
2-Exotoxins:
-phospholipase D(PLD)
Membrane active cytotoxic toxin
-Serine protease
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4-Disease produced by c.ovis:
biotypespeciesdiseaseEquine biotypeHorseUlcerative lymphangitiscattleOedematous skin diseaseOvine biotypeSheep and goatCaseous lymphadenitis -
5-Lab.diagnosis of c.ovis:
Sampling:
Samples collected depends on the clinical condition.
-pus in case of suppurative lesions.
1-Direct microscopic examination of Gram stained smear
(Gram positive pleomorphic bacili)
2-Isolation of bacteria
Culture requirements
facultative anaerobic(37c and PH 7.2-7.4)
Media used for isolation
(blood agar:small whitsh colonies surrounded by narrow zone of beta hemolysis)
3-Identification by:
-Colonial characteristics
-Biochemical reactions.
-Animal inoculation(strauss reaction)
-serodiagnosis
-PCR
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6-Treatment:
-carried out by prolonged penicillin therapy
-but antibiotic treat in sheep,goat is ineffective
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Corynebacterium renale group
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3serotypes:
1-c.renale(type|)
2-c.pilosum(type||)
3-c.cystitidis(type|||)
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1-Morphology:
2-culture characteristics:
-Facultative anaerobic
(with optimum temp is 37c and PH is neutral)
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**Media for isolation**
-Blood agar non hemolytic
-pigment production is variable:
c.renalePale yellow coloniesc.pilosumYellow coloniesc.cystitidisWhite colonies -
3-Biochemical reactions:--catalase positive.
-strong urease-positive.
-CAMP test positive.
4-Disease produced by c.renale group:
cattleCystitis and pyelonephritissheepUlceratie balanoposthitis -
5-Lab.diagnosis of c.renale:
Sample collected is mid-stream urine.
1-Direct microscopic examination of Gram stained smear
(Gram positive pleomorphic bacili)
2-Isolation of bacteria
Media used for isolation
(blood agar:small non hemolytic colonies after 24 hours)
-pigment production is evident after 48 hours
3-Identification by:
Hemolysis,pigment production)) -Colonial characteristics
-Biochemical reactions.
(catalase positive ,strong urease positive)
-serodiagnosis
-PCR
(specific primers)
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6-Treatment:
-carried out by prolonged penicillin therapy
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Genus:Rhodococcus
R.equi
(c.equi) -
1-Morphology:
-G+ve coccobacili.
-Non motile.
-Non spore forming.
-capsulated
2-culture characteristics:
-Facultative anaerobic
(with optimum temp is 37c and PH is 7.2-7.5)
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**Media for isolation**
-Blood agar non hemolytic
-colonies are mucoid salmon pink
3-Biochemical reactions:-
-catalase-positive.
-ferment glucose and maltose.
-Nitrare reduction-positive.
-Urease-positive.
Variability:-
27 capsular serotype
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4-Disease produced by R.equi:-
-Infect young foals producing suppurative pneumonia and pulmonary abscessation.
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5-Lab.diagnosis of R.equi:-
Samples collected pus from suppurative lesions.
1-Direct microscopic examination of Gram stained smear.
(Gram positive coccobacili)
2-Isolation of bacteria
Media used for isolation
blood agar: non hemolytic colonies). )
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3-Identification by:
-Colonial characteristics
-Biochemical reactions.
(catalase positive , urease positive, not ferment sugars)
-serodiagnosis
-PCR
(using specific primers)
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6-Treatment:
-preferable combined treatment of erythromycin and rifampicin.
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Genus:Arcanobacterium
A.pyogenes -
1-Morphology:
-G+ve pleomorphic bacili.
-Non motile.
-Non spore forming.
-Non capsulated.
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2-culture characteristics:
-Facultative anaerobic
(with optimum temp is 37c and PH is neutral)
-Fastidious bacteria require enriched media.
-Blood agar beta hemolysis
-Colonies are translucent pin point
**Media for isolation**
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3-Biochemical reactions:-
-catalase-negative.
-Ferment lactose.
-Liquefy gelatin.
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-:virulance factors
1-cell wall.
2-Exotoxin:-
-pyolysin O(PLO)
-(acholestrol dependent cytolysin)
-Neuraminidase(for adhesion)
3-Miscellaneous productus as Dnase.
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4-Disease produced by A.pyogenes:-
-Purulent infections of
lung,liver,joints,uterus and udder(summer mastitis)
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5-Lab.diagnosis of A.pyogenes:-
-samples collected from(milk,pus or exudate).
1-Direct microscopic examination of Gram stained smear.
(G+ve pleomorphic bacili arranged in Chinese letters)
2-Isolation of bacteria
Culture requirement
(facultative anaerobic,37C and PH=7.2-7.4)
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Media used for isolation:-
Blood agar:48hours pin point colonies surrounded by asmall zone of beta hemolytic.
3-Identification by:
-Colonial characteristics
-Biochemical reactions.
(catalase negative,ferment lactose)
-serodiagnosis
-PCR
Using specific primers
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6-Treatment:-
-Drainge of abscess.
-Antimicrobial therapy by pencillin.
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Thank you very much
With my best wishes:
By:Aya Mohamed