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DC LIU CHA ANTHRANOID

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PHAN T

Tn khoa hc: PTD la hep Senna angustifolia Mill. = Cassia angustifolia Vahl., Phn h Ceasalpinioidae, H Fabaceae PTD la nhn Senna acutifolia (Del.) = Cassia acutifolia Del. Phn h Ceasalpinioidae, H Fabaceae Nhiu tac gia xem hai loi nay la mt: Senna angustifolia Mill.

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C. angustifolia C. acutifolia

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PHAN T

- B phn dng: l cht (Folium Sennae).

- Cn dng ht

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THNH PHN HA HC

Anthranoid chim 2 - 3 %.

-Dng t do: rhein, aloe emodin, chrysophanol

-Dng glycosid:

R1 = R2 = H cc sennidin

- R3 = COOH, trans : Sennidin A

- R3 = COOH, cis : Sennidin B

- R3 = CH2OH, trans : Sennidin C

- R3 = CH2OH, cis : Sennidin D

R1 = R2 = Glu cc Sennosid tng ng

OH

COOH

R3

OHOR1

OR2

O

O

HH

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THNH PHN HA HC

-Cc flavonoid: kaempferol, isorhamnetin.

-Cht nha !!!!

Kaempferol

OH O

CH3

CH3

Glu

OH

Tinnevellin glucosid

INH TINH (SKLM) AG: n-propanol EA nc (4:40:30) AQ: benzen acid acetic (80:20)

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NH LNG - Dch chit (nc) loi tp chuyn sang dng

oxy ha thy phn thu dch anthraquinon/KOH UV 500 nm

- Cht chun: 1,8-dihydroxyanthraquinon

- Hm lng tnh theo sennosid

1 mg 1,8-dihydroxyanthraquinon = 1.797 mg sennosid

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TC DNG - CNG DNG

- Ngi Rp dng t TK IX, sau nhp sang Chu u, hin nay rt ph bin

- Liu dng:

- 2 g: tr tiu ha

- 3 - 4 g: nhun trng

- 5 - 7 g: ty x

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MUNG TRU

Tn khc: Mung lc, cy lc,

Tn khoa hc: Senna alata (L.) Roxb., Fabaceae

= Cassia alata L.,

BPD: L (Folium Cassiae), ht (Semen Cassiae)

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THNH PHN HA HC Trong l, qua, r u c anthranoid:

L

AQ

chrysophanol, aloe emodin, rhein,

emodin.

AG

Rhein-8-glucosid, physcion-8-glucosid

Aloe-emodin-8-glucosid

Flavo Kaempferol

O

O

OH

COOH

OGlu

Rhein-8-glucosid

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Qua

AQ

chrysophanol, aloe emodin, rhein,

emodin.

AG

Rhein-8-glucosid, Aloe-emodin-8-

glucosid

Flavo Kaempferol

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TC DNG - CNG DNG

- Dng cha lc: gi nt p ln ch nhim

- Cha to bn: dng 4 - 5 g l dng thuc sc

- Cha giun a: phi hp vi ht Trm bu

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Dc in Vit Nam IV qui nh:

Rheum palmatum L., Polygonaceae

Rheum officinale Baillon, Polygonaceae

Hoc ging lai ca 2 loi trn.

palmatum: hnh bn tay

officinale: dc dng

I HONG

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Cy thao ln (2m).

L hnh tim to (30-40cm), c b cha (Polygonaceae)

R. palmatum c thy su hn R. officinale.

Sau 3-4 nm mc 1 ngn thn hnh chy mang nhiu hoa.

Cy a m, mt. Mc cao >1000m. Tt nht 2200-4000m.

C ngun gc t Trung Quc. Vit Nam cn phai nhp.

I HONG

M T THC VT

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Rheum sp

21 21 Rheum palmatum

22 22 Rheum palmatum

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Rheum palmatum

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B phn dng: thn r Rhizoma Rhei

Thn r nhng cy t 3-4 nm tui.

Ma thu, khi cy tn li

Ct b r, thn r gt b v ngoi.

Phi hay sy kh

Ct gi 1 nm mi dng.

Mt s ni cn dng cung l lm bnh.

I HONG

THU HI CH BIN

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I HONG

M T DC LIU

DVN III

Ming hnh da, tr hay ovan

10cm

Mt ngoi mu vng nu, i khi c nhng am en nht

DVN IV

Hnh tr, hnh non, dng cu hay meo mo khng u hay nhng phin mng

= 3-10cm

Mt ngoi mu nu vng hay nu , i khi c nhng m en nht. Phin c th c nhng sc en

Vt b c mu cam

Mi c trng

V ng cht

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Rhizoma Rhei

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1. manh ban 2. manh mo mem

3. si

4. calci oxalat

hnh cau gai

5. hat tinh bot

Soi bt

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Anthranoid 3-5%

Tannin 5-12%, PC (khng thy phn) > PG

V c: Calci oxalat

Tinh bt, pectin, nha

Tannin coi nh tp cht khi nghin cu anthranoid, d tan trong nc, cn (dung mi thng dng chit xut Anthranoid)

I HONG

THNH PHN HA HC

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AQ t do 1-2 gm: chrysophanol, emodin, physcion, aloe

emodin v rhein.

AG chim 60-70% anthranoid ton phn.

AG ca cc dn cht kh (lu kho 1 nm)

Cc dianthron: cac rheidin, cac palmidin,

I HONG THNH PHN HA HC

OH O

O

OH

OH OH

COOH

COOH

OH O

O

OH

OH OH

COOH

OH CH3

Dirhein Rheidin A

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Phan ng Borntrager:

0.10g bt + 5ml H2SO4 (BM).

Lc

Lc vi Benzen, Ether (DVN III), cloroform (DVN

IV), dicloromethan (PTN DLiu)

c mu vng.

Ly lp dung mi hu c, lc vi KOH 0.1N, amoniac

10% (DVN). lp kim c mu .

I HONG

NH TNH

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Sc k lp mng:

Pha tnh: silica gel G hot ha 105oC (DVN III), silica gel F 254 (DVN IV, PTN Dliu).

Pha ng: EP-EA-acid formic (75:25:1) (DVN), Bz-EA-a. formic (75:24:1) (PTN Dliu).

Dung dch th:

Chit bng nc acid. Lc

Lc vi ether ethylic, c n cn

Ha li trong ether

Chm sc k

I HONG

NH TNH

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I HONG

Sc k lp mng:

Dung dch i chiu: emodin/ether ethylic nng 1mg/ml hoc dch chit bt H chun.

Chm trn cng ban mng.

Quan st di UV 365nm.

H ban mng trong hi amoniac.

I HONG

NH TNH

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Pht hin bng SKLM.

Pha tnh: Silica gel F254 Pha ng: EA-MeOH-H2O (100:3.5:10)

Dung dch th: chit bng ethnol, BM. Lc, chm ln ban mng 10L.

Quan st UV 365nm.

Khng c c vt pht hunh quang tm xanh khoang Rf 0.4-0.6 (vt ca rhaponticosid)

I HONG

TP CHT (DVN IV)

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Rhaponticosid xut hin khi co ln tp cac loai: R. rhaponticum hay R. undulatum.

Rhaponticosid phat hunh quang UV 365.

Co th kim tra bng SKLM vi h n-butanol-a.acetic-nc (4:1:5).

I HONG TP CHT

hRf = 50 55 70 80 95

C

T

mau th T a b lan rhaponticosid

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6.1. Phng phap Auterhoff

- thuy phan trc tiep vi AcOH bang, un soi 15 phut

- e nguoi, tach aglycon bang ether soi.

- aglycon cho phan ng vi (NaOH + NH3)

- tach lp kiem (mau o), em o quang (515 nm)

- So sanh vi dung dch CoCl2

- (A cua d.dch CoCl2 1% # A cua d.dch istizin 3,6 ppm)

ai hoang phai cha 2,5% anthranoid, tnh theo Istizin

NH LNG

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- chiet m (g) mau bang nc nong cach thuy.

- e nguoi, them Na bicarbonat, lac, loc lay dch

- Oxy hoa bang FeCl3 2% nong, thuy phan (HCl nong)

- chiet aglycon bang ether, can ether + Mg acetat / MeOH

- o o hap thu A 515 nm (khong dung chuan)

- ket qua c tnh trc tiep X% = 64A / 100m

ai hoang phai cha 2,2% anthranoid (tnh theo rhein)

Thc chat : nh lng cac anthranoid acid

6.2. Phng phap DVN IV

NH LNG

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I HONG

Tng tit dch: tng thai nc, Na v K

Tc dng ln h c trn

Lm tng nhu ng rut nhun, ty.

Tng nhu ng bng quang, t cung PNCT, b vim bng quang khng nn dng.

Gy sung huyt CC ngi b tr.

Cha nhiu dng kh khng dng DL ti.

Nhiu Calci oxalat thn trng/si thn.

Ung c tc dng chm: 8-12 gi.

I HONG

TC DNG

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I HONG

Tc dng mnh dn theo liu lng:

0.05-0.1g : b, kch thch tiu ha.

0.1-0.15g : nhun trng.

0.5-2g : x.

I HONG

TC DNG

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NHU

Tn khac: nhau nui, cy ngao, cy mt quy , Giu

Tn khoa hoc: Morinda citrifolia L., Rubiaceae

BPD: Toan cy (r, vo thn, la va qua), nhng chu yu la

r.

Qua cng ang c lu y nhiu.

Cng dung:

Cha cao huyt ap, au lng nhc mi.

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NHAU

Ten khac: Cay ngao, Nhau nui, Mat quy

Ten khoa hoc: Morinda citrifolia L.

Ho ca phe (Rubiaceae)

Mo ta thc vat

Cay cao khoang 6-8 met.

La moc oi, phien la to, hnh bau duc, tren bong lang.

Qua phc. Qua co v chua hi khai, an c.

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Nhau nc: Morinda persicaefolia Ham., Rubiaceae

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Damnacanthal Nor-damnacanthal

Cac cht co tac dng khang khi u trong r Nhau nui

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H TH

Tn khoa hc: Fallopia multiflora Haraldson

(Polygonum multiforum Thunb.), Polygonaceae

M ta:

Dy leo, la n hinh tim,

R c mau nu o to va nc

B phn dung: r c (Radix Poligoni multiflori)

Thu hai ch bin: ao c vao mua ng, cu chng cu sai

Cng dng:

Lam thuc b mau, cha ru toc bc sm

Cha suy nhc thn kinh, mt ngu hay qun.

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L HI - Tn khac: Tng am, h thit, Nha am

- Tn khoa hc: Aloe sp., Asphodelaceae (Liliaceae, Aloeaceae)

Hai loai thng dung: Aloe vera L. va Aloe ferox Mill.

- Aloe ferox Mill. cao t 2 - 5m, l mc thnh hoa th dy, di 15 -50cm, rng 10cm gc, c gai mt di l v mp l. Hoa mu . Ch yu c nam Phi, cho l hi x Cape

- Aloe vera L. (= vulgaris Lam.) thn ngn 30 - 50cm. L ch c gai 2 mp. Hoa mu vng. Cy ngun gc bc Phi, cho l hi Barbade.

- BPD: La dung ti hay ly nha th

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Aloe ferox Mill.

Aloe vera L.

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Aloe ferox Mill.

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Cac anthranoid co trong mt s loai Aloe

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Cac C-Glycosid

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TC DNG - CNG DNG

- Kich thich tiu hoa ty x tuy liu lng

- Cht nhy dung tr bng, dung trong my phm

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HT