Download - 分子生物学 仪器介绍及应用
Template
2013.03. 14 XTBGXTBG1.
Illumina Miseq2.
3.
PCR4.
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6.
Miseq
NightSHADE
Roche LightCycler 480II
THERMOFISHER Varioskan FlashCFlow
BIO-RAD ChemiDocXRS
PERCIVAL LED
Thermo Fisher PICO-21Tomy-SX500
Bionano
Thermo Fisher
Bio-RAD PCR
28
NightSHADE
XTBG8NightSHADE LB 985
CCDLEDluciferaseGFP9
tch4Plant Physiology vol 130 ,2002, p770-783TCH4, ,TCH4LUC11
121264813
Promoter Gene XLuciferase Genetranscript
XLuciferaseProtein XmRNA Luciferase
LUC
LUClucDNAATP16
CAB2 48hPaul F. Devlin: Signs of the time: environmental input to the circadian clock Journal of Experimental Botany, Vol.53,No.374.pp.1538-1550.July1,2002CAB2CAB217
Col- 03CCD4indiGOCCD Side mounting of camera
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Side mounting of camera
Side view of plant growing chambers
Plant Cell, Arabidopsis FHY3 Specifically Gates Phytochrome Signaling to Circadian Clock, 2006
Berthold FHY3(far-red elongated hypocotyl3)6CAB2-LUC1629Plant Cell, Arabidopsis FHY3 Specifically Gates Phytochrome Signaling toCircadian Clock, 200622
ELENA V. DOUKHANINA et.al , 2006
Expression of Human Nuclear Receptors in Plants for the Discovery of Plant-Derived Ligands25
Illumina Miseq System
XTBG27Illumina Miseqwww.themegallery.comCompany LogoIllumina Technology OverviewMiSeq IntroductionPerformanceApplications16S rDNAV(de-novo sequencing)Small RNA
Illumina Miseq
Illumina Miseq
135%201%300.1%Q20Q3020301G0.9G20Q2090%30 16s rRNAV(V3\V4),
1.16S rDNAV16s rRNAV(V3\V4)3116S rRNA Structure
Miseq 16SrRNA16S9Miseq16SV4254bp,bp32V4Liu Z, Lozupone C et.al 2007. Short pyrosequencing reads suffice for accurate microbial community analysis.
V4Miseq150bp250bpV446bpV4V4P5P724V433Phylum-level
MiseqHiseq2000read 1 Miseq34DNADNA N2Biorupter-UDC-200NexteraIllumina Miseq 16SrRNA V435
Bionano Qubit 2.0 Fluorometer
DNA A260=1.8-2.0DNA ug/ulinput DNADNA 300bp