DNA

DNA

DNA

DNAPRIMASEDNARNA2

DNA

DNA

SSB

nucleasesDNase RNase3-5RNaseH5-3

DNADNADNA53ATPDNADNA4DNAI

MCM2-7MCM2-7DNAORCCDc6Cdt1DNA G1-SCDc45CDc7Cdk2MCMORCXENOPUSDNADNA80bpORCMCM11DNAMCMMCMCDc45DNAMCMDNA

CDc7Cdk2 CDc45DNAMCM2-7

DNA

G1

DNA

ORCMCM82-bpDNA

MCMDNAORCDNA

MCM

DNACDC45DNA

MCMCDC7

CDC45D

DNA

MCM82bpDNAORC80bpDNAG1MCMDNADNA82bpMCMORCMCMORCDNAMCMDNACDC6CDT1 ORCCDC6CDT1DNAMCMMCMORCDNAORCMCM

MCMCDC7MCM4CDC7DCDC45MCM1MCMCDC45DNACDC45MCMMCM106MCMMCMG1GI/S

G1SG2GIG0RDNA

DNADNADNADNADNA RNARF-CDNAPCNADNA 5DNADNA NDADNADNADNAATPDNA5-33-5RFDNADNAI /II /DNAPol:,ssRP-A1CDC CDK MCM DNADNAORCORCDNAMMCM2-7SSSFP2 CDc7SCDKDNAORCMCM1DNAMCMORC22-63kbDNAPCR202DNAMCMORC24GEMININMCM3MCMCDT15NPECDc456P27KIPCDc45CDK2c45CDK2ORCMCMDNA#A&B251 bpPCRbead beads25115412094670 bpDNADNAbead2%ABCDNA beads1-56-10Geminin D67 bpDNAMCM94bpMCMOCR=11 D82bpMCMA0.3-6kbORC10-15kbORCMCM3DNAGemininMCMDNAORCDNADNAB6kbDNAMCM3ORC220-401.CMCM2-7MCM31kbPCR100bpDNAORC21kb 100bpMCM3100bpMCM7MCM4MCM3MCM2-7DNAD1kbMCM3ORC2111MCM3DNA90bp

ORCORCMCM2-7DNAORCMCM6kbORCMCM100bpMCM1M KCL1kb100bpDNAMCMDNAORC0.6M KCLDNADNAORCMCMCCDC-45DNACDK-2ORC2DNAMCMCDC45MCMCDC45DNAMCM3ORC2CDC45CDC45ORC2MCM3ABCDC45DNADNACDC45CDC45CMCMDNAMCM50%DNAMCM6%NPEDNAMCMMCM4CDK2MCM4NPECDC76MCM3ORC22016MCM4NPE42CDC7MCM2-7DNAMCMCDC7MCN2-7CDC45MCMCDC45MCMCDC45MCMCDC45MCMCDC45CDC45CDK2MCM2-7CDC7MCM