Download - Dr.Chen Gao cg_npwr@yahoo
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
Dr.Chen Gao
DNA的提取与含量测定
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
DNA ， DNP
变性蛋白，肝糜
氯仿
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
( 二 ) 二苯胺法测定DNA含量
二、原理 核酸含量的测定方法包括紫外吸收法、定磷法和
分别针对 DNA和 RNA的颜色反应方法
本实验采用针对 DNA的二苯胺法测 DNA的含量。在酸性溶液中， DNA与二苯胺共热生成蓝色化合物，该物质在 595nm有最大吸收，在 40～ 400mg/mL范围内其峰值与 DNA含量成正比
NH
100℃
冰醋酸，少量硫酸蓝色物DNA +
