Dr. Sc., Ph.D., Senior specialist

Moscow Regional Research and Clinical Moscow Regional Research and Clinical Institute “MONIKI” named after Institute “MONIKI” named after

M.F.VladimirskyM.F.Vladimirsky

PhotonicsPhotonics

Speaker: Dmitrii Rogatkin Speaker: Dmitrii Rogatkin

Moscow, RFMoscow, RF

Agenda / contentAgenda / contentAgenda / contentAgenda / content

1. Description of the Institute

2. Description of the research activities and used photonic technique

3. General medical problem taken into consideration where co-operation is sought

4. Project ideas, our experience and background

5. Summary

1. Description of the Institute

2. Description of the research activities and used photonic technique

3. General medical problem taken into consideration where co-operation is sought

4. Project ideas, our experience and background

5. Summary

Moscow Regional Research and Clinical InstituteMoscow Regional Research and Clinical Institute ««MONIKIMONIKI»» named after M.F.Vladimirskiy:named after M.F.Vladimirskiy:

1. Description of the Institute1. Description of the Institute1. Description of the Institute1. Description of the Institute

- One of the oldest State medical institute in Russia;

- Was founded in 1773;

- Multidisciplinary institute;

- Around 2000 employees;

- Around 300 researchers;

- Around 100 Ph.D. and Dr. Sc.;

- 3 Members of Russian Academy of Science;

- Budget per year is around 150 million USD.

2. Research activities and used 2. Research activities and used photonic techniquephotonic technique

2. Research activities and used 2. Research activities and used photonic techniquephotonic technique

- Fundamental and applied research in most main areas of medicine:Oncology, Gastroenterology, Radiology, Surgery, Therapy, etc.

- More than 50 scientific projects, programs and contracts per year;

- More than 1000 publications per year.

Laser Noninvasive Laser Noninvasive Medical Medical

SpectrophotometrySpectrophotometry

Laser Doppler Flowmetry (LDF)

Tissue Reflectance Oximetry (TRO)

Laser Fluorescent Diagnostics (LFD)

General:General:

Photonics:Photonics:

Tested Volume (U)

U 2-3 mm3

Ner 30000 un.

Laser Doppler Flowmetry (LDF) Technique

Perfusion (pf. un.):Im = Ner V

arterioles2–3 mm/s

capillaries0,3 mm/s

venules2,5 mm/s

Velocity, V

Average magnitude Im

Аctive and passive regulation processes

(frequency rhythms) in a microcirculation system

Tissue Reflectance Oximetry (TRO) technique

Hb

HbO2

H2O

Melanin

Other

Wavelength, nm400 550 700 850 , nm

a, mm-1

10

1

10-1

10-2

10-3

10-4

Arterio-venules anastomosis

venulescapillaries

arterioles

SO2 =

CHbO2

CHbO2 + CHb

Vb =

CHbO2+Hb

CHbO2+Hb + Cother CHb

Evaluation of tissue’s SO2 in TRO

Optical probe

2-3 mm

SO2a

SO2v

tissue’s SO2

SO2

Sampling volume

Laser fluorescent diagnostics (LFD)

laser

tissue

Backscattering and fluorescent spectra

-- NADH -- FP -- Lipopigments -- Porphyrin -- others

Content in tissue:

Center of tumor

Intact tissue

Standard scatter Acc

umul

atio

n of

por

phyr

ins

4. General medical problem which 4. General medical problem which is taken into consideration and is taken into consideration and where co-operation is soughtwhere co-operation is sought

Biophotonics:Biophotonics: noninvasive laser diagnostics in radiology

We are looking for the European partner(s) to study the efficiency of the complex laser noninvasive diagnostic technique in radiology and oncology.

Medical specialists and research teams are Medical specialists and research teams are preferable.preferable.

Radiotherapy of tumors and prognosis of efficacy

1,8 Gy. х 2 times per day 1,8 Gy. х 2 times per day

Total Doze - 69,6 Gy

Schedule of Dynamic Multifractioning of Irradiation Doze

Pause14 days

Others

Head and neck cancer

%

%28,3

Direct nearest positive result

Without nearest positive visible results

Result ?

Continue ?

Surgery intervention ?

%

%

75

5. Project ideas, our experience 5. Project ideas, our experience and backgroundand background

Photonics equipment:Photonics equipment:

Multifunctional laser noninvasive diagnostic system (MLNDS)

Oral cavity cancers

Skin cancersPilot model of MLNDS

Preliminary results by LFDEfficacy of radiotherapy of oral cavity cancers in depend on initial fluorescence of porphyrins and flavins in the tumor

Initial coefficients of fluorescent contrast (Kf) Number of cases Flavins,

normal mucosa Porphyrins,

normal mucosa Flavins, tumor

Porphyrins, tumor

Normal tissues 20 0,520,23 0,100,06 - -

Positive dynamics of radiotherapy

14 0,500,21 0,160,09 0,430,20 0,250,13

Absence of positive

dynamics 6 0,510,25 0,180,1 0,120,09 0,780,35

Preliminary results by TRO:

SO2sp=(100-SO2)/Vb

Evaluation of oxygen status of a tumor(oxygen uptake in tumor)

SO2m=SO2/Im

Characteristics of a tumor

S02spsurround. tissue

S02sptumor

Ratiotumor/surround.

Nearest outcome of radiotherapy

T1N0M0 1,6 0,69 0,43 Full regression

T2N0M0 1,28 0,26 0,2 Full regression

T2N0M0 0,57 0,07 0,12 Regression 90%

T3N1M0 1,02 0,09 0,09Weak dynamics, regression 50%

Tumor perfusion before and after ionizing irradiation procedure (distant photon therapy (DPT))

1. Before irradiation 2. 30 min after irradiation

Preliminary results by LDF:

6. Summary6. Summary

1. Title of the Project: Complex noninvasive laser medical diagnostics in the modern radiology.

2. Main idea of the Project – clinical research in a field of radiology and oncology to study an efficacy of laser noninvasive spectrophotometry to predict a positive radiotreatment outcome.

3. Duration time of the Project – 2-3 years.

4. It is necessary for the Project to have several clinic international platforms for independent double (triple) reliable study.

Biophotonics medical Project:Biophotonics medical Project:

Thank you !Thank you !Thank you !Thank you !

Contact details:Contact details:

Dr. Dmitrii Rogatkin

“MONIKI”, Shepkina str. 61/2, Moscow, RF

rogatkin@monikiweb.ru

www.monikiweb.ru

Tel. +7-495-631-73-17

Tel. +7-985-767-01-38