dti lecture 100710
TRANSCRIPT
Introduction to Diffusion Tensor Imaging
Why DTI? Diffusion – what it is, how it affects MR
signal Tensor – how we represent diffusion Imaging – how we measure it in MRI
Stroke/ischemia Alzheimer’s Disease Multiple Sclerosis Brain maturation studies Ischemia and stroke Neoplasm Preoperative planning Traumatic brain injury Congenital anomalies and
diseases of white matter Encephalopathies Neurodegenerative diseases Spinal Cord Injury Epilepsy Dementia, schizophrenia,
depression Developmental disorders Autism Aging
Why diffusion?
http://www.vh.org/Providers/Textbooks/BrainAnatomy/Ch5Text/Section18.html
http://eclipse.nichd.nih.gov/nichd/DTMRI/mri/
Conceptually: in vivo histology
Why diffusion? Diffusion is EXTREMELY SENSITIVE to
differences and changes in tissue microstructure Myelination/Demyelination Axon damage/loss Inflammation/Edema Necrosis
It is NOT a biomarker of white matter integrity
It is NOT just about white matter Gray matter Cardiac tissue
Example DTI image
“Fractional Anisotropy” map “map” is a computed
parameter, unlike an “image” which is acquired signal
Also called a “tractogram” since it clearly shows major white matter fiber tracts
What is Diffusion?
stochastic movement of particles in a solvent, driven by the thermal molecular motion of the solvent…
… and also applies to motion of the solvent itself (Einstein, 1905)
time NOTE: In the limit N→∞, use the Central Limit Theorem to assume “step size” is fixed and equal to the average of individual displacements i.
1D Fick’s Law - what the flux?
t = t0 +
x
t = t0
0 2x 0x 0x 0x0 2x
t = t0 +
x
t = t0
0 2x 0x 0x 0x0 2x
What is the flux (J) through x0 after one time interval ?
C1(x)C2(x)
dxdCJ
2
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Adolf Fick, 1855: Flux is proportional to the particle concentration gradient(conservation of mass)
The Diffusion Coefficient
3D Fick’s Law Note the minus sign: flux
goes from high to low concentration
del operator replaces partial derivative
factor of 6, not 2 (why?) D is the diffusion
coefficient This is the expression for
isotropic diffusion
62D
CDJ
CJ
6
2
Isotropic Diffusion (water)
water
ink
Dtr 6
r1
t1
r2
t2
62D
Diffusion in Tissue (Anisotropic)
t
ink
r2
r3
r1
diffusionellipsoid
tDr 11 2
tDr 22 2
tDr 33 2x
y
z
laboratoryframe
DON’T try this
at lab!!!!!
The Diffusion Tensor
zzyzxz
yzyyxy
xzxyxx
DDDDDDDDD
x
y
z
r2
r3
r1
3
2
1
000000
DD
Ddiagonalization
Lab frame Intrinsic frame
Tensor Invariants
Eigenvalues: diagonalization (iterative QR factorization)
Eigenvectors
xx xy xz
xy yy yz
xz yz zz
D D DD D DD D D
1 2 3D D D
321 eee
Tensor Invariants
Shape invariants: analytical calculation directly from tensor coeffs
xx xy xz
xy yy yz
xz yz zz
D D DD D DD D D
13av xx yy zzD D D D
12
2 2 2
13
xx yy xx zz yy zz
surfxy xz yz
D D D D D DD
D D D
12 3
2 22xx yy zz xx yz
volxy xz yz zz xy yy xz
D D D D DD
D D D D D D D
Scalar Anisotropy Indices
avND
D
DADC
DDDADC
32131
2
2
23
22
21
23
22
21
1
23
mag
surfND
D
DD
FA
DDD
DDDDDDFA
FA vs. ADC
FAx 10 -4 )
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
Prob
abili
ty
0.0000
0.0002
0.0004
0.0006
0.0008
0.0010
WMGMCSF
MD x 10 -3 mm2/s)
0 500 1000 1500 2000 2500 3000 3500 4000
Prob
abili
ty
0.000
0.001
0.002
0.003
0.004
0.005
WMGMCSF
FA and ADC are very useful clinically, but are very different.
Tensor has a LOT of information!
Q. Which metric would you use to detect brain cancer?
Vector anisotropy measures
We can use eigenvector information from the tensor as well Represent direction of
primary eigenvector as color on a scalar map
Or render the primary eigenvectors as “fibers” for astonishing* 3D visualizations
Red = R/LGreen = A/P Blue = S/I
*but how “real” is it? Many PhD theses have asked….
Diffusion tensor coefficients Diffusion tensor invariants
Scalar anisotropy indices
Vector anisotropy indices
Effect of Diffusion on MRI signal
Signal attenuation!
Diffusion term
Diffusion weighted MRI
G G
echo
2
0
δexp δ 3M G DM
2 δδ 3b G
(boxcar gradients)“b-value”
Consider simplified diffusion experiment…
MR Measurement of Diffusion Tensor
jTj j
j
pG G q
r
0
xx xy xz j
j j j xy yy yz j
xz yz zz j
D D D pp q r D D D q
D D D rj
b
S S e
22 2γ δ Δ δ 3b G
1
6j NN
jth diffusion-weighted
image
Diffusion magnitude
Diffusion direction
Gz
Gy
Gx
...
...
...
Solving for D
20
0
xx xy xz j
j j j xy yy yz j
xz yz zz j
D D D pp q r D D D q
D D D rj
b
S S e
1. Acquire T2W image (b = 0 s/mm2)
3. Choose a diffusion gradient orientation2. Choose a b-value
4. Acquire image (Sj)5. Repeat steps 1 – 3, j = 1 … N times
6. Solve for D…. How?
Let’s do some linear algebra…
zj
yj
xj
zzzyzx
yzyyyx
xzxyxx
zjyjxjj
DDDDDDDDD
bSS
exp0
yz
xz
xy
xx
yy
xx
T
jxy
jxy
jxy
jzz
jyy
jxx
j
DDDDDD
bSS
222
ln2
2
2
01661 xNxNx xAY
B-matrix formalism
22
yzyzxzxzxyxyzzzzyyyyxxxx DDDDDDb 222222
yzyzxzxzxyxyzzzzyyyyxxxx DbDbDbDbDbDb 222
3
1
3
1i jijij Db
The “b-matrix”
The b-matrix formalism summarizes total attenuating effect of all gradient waveforms in all directions (including imaging gradients)
T2W(b = 0 s/mm2)
Y, -ZY, Z-X, Y
X, Y-X, Z+X, Z
24
SVD
DIAG
T2W(b = 0 s/mm2)
…DWI
(j = 1, 2, 3 … N)
Dij
N=27 N=55
N=13N=6
N NEX # DWI6 8 5613 4 5627 2 5655 1 56
#DWI = (N + 1) x NEX
If TR = 4 sec, then acq time = 56*4sec = 3.7 minutes
Tradeoff: N vs NEX
Rotational invariance
Hasan et al, JMRI 2001Jones MRM 2004
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Empirical Image Qualityincreasing N, decreasing NEX
incr
eas i
ng b
-va l
ue
How low can you go?
High b-values mean more attenuation, lower SNR
Lower b-values mean higher SNR, room for more N
At very low b-values, imaging gradients’ diffusion effects are no longer negligible
Lower b-values also do not probe same diffusion scale, less clinically interesting
b=100 s/mm2 b=500 s/mm2
(N = 6, 8 NEX)
Echo-Planar Imaging (EPI)
Advantages Minimal motion
artifacts NEX N
Disadvantages Eddy current artifacts T2* limits spatial
resolution Geometric distortion
(susceptibility)
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DT-MRI Alexander
SLFSLF CRCR CCCC CINGCING
Partial Volume Effects on Anisotropy
DT-MRI Alexander
Mapping Complex Diffusion
Based Upon Q-Space Theory – Model Independent ODF – orientation density function (Tuch et al., Neuron 2003)
Diffusion Spectrum Imaging (DSI) (Tuch et al. Neuron 2003, Wedeen et al. 2005)
High Angular Diffusion Imaging (HARDI), Q-Ball (Frank 2002; Tuch et al. Neuron 2003)