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سورة سورة غافر غافر

International Commission for Microbiological International Commission for Microbiological Specifications for Foods (ICMSF, 2002).Specifications for Foods (ICMSF, 2002).

E. sakazakii as severe hazard for restricted E. sakazakii as severe hazard for restricted population, life threatening or substantial chronic population, life threatening or substantial chronic sequelae or long duration.sequelae or long duration.

World Health OrganizationWorld Health Organization

(WHO)(WHO)

•Can grow at temp. as low as 5.5 Can grow at temp. as low as 5.5 ooC.C.

•One of the most thermo tolerant bacteria in the One of the most thermo tolerant bacteria in the

family Enterobacteriace.family Enterobacteriace.

•Has high tolerance to osmotic stress and Has high tolerance to osmotic stress and

desiccation.desiccation.

Antiviral , Antiallergic ,AntiinflammatoryAntiviral , Antiallergic ,Antiinflammatory

Antitumor Antitumor

Antioxidant Antioxidant

Immunostimulant, Immunostimulant, ImmanoregulatorImmanoregulator

Antibacterial Antibacterial

A - Preparation of samplesA - Preparation of samples::

B - Isolation of E. Sakazakii and B - Isolation of E. Sakazakii and

other Enterobacter species: other Enterobacter species:

http://www.ars.usda.gov/is/graphics/photos/mar05/d034-1.htm

Examined samplesExamined samplesE. sakazakiiE. sakazakiiE. agglomeransE. agglomeransE. gergoviaeE. gergoviaeE. cloacaeE. cloacaeE. aerogenesE. aerogenes

NONO%%NONO%%NONO%%NONO%%NONO%%

Raw milkRaw milk-Dairy shops-Dairy shops-Farm milk-Farm milk-Streat vendors-Streat vendors-Farmer's houses-Farmer's houses

--11--33

--6.66.6--

2020

44----66

26.626.6----

4040

--------

--------

11223333

6.66.613.313.320202020

55----11

33.333.3----

6.66.6

UHT milkUHT milkChocolate milk mixChocolate milk mixStrauberry milk mixStrauberry milk mix

11--22

1010--

2020

11--22

1010--

2020

----11

----

1010

443322

404030302020

1133--

10103030--

Kareish cheeseKareish cheeseDamietta cheeseDamietta cheese

7766

23.323.32020

33--

1010--

----

----

5511

16.616.63.33.3

44--

13.313.3--

Ice creamIce cream-Large scale -Large scale producersproducers-Small scale -Small scale producersproducers-Streat vendors-Streat vendors

114433

101040403030

22--22

2020--

2020

11----

1010----

2255--

20205050--

11----

1010--

1010

Total )180(Total )180(282815.515.5202011.111.1221.11.1313117.217.216168.88.8

Table 1: Incidence of Enterobacter species in the examined milk and some milk products Table 1: Incidence of Enterobacter species in the examined milk and some milk products samplessamples . .

StorageStorageTime/dayTime/dayControlControl

Fresh MushroomFresh MushroomPreserved MushroomPreserved Mushroom

PHPH1%1%5%5%10%10%1%1%5%5%10%10%

Finished Finished cheesecheese3.6× 103.6× 10666.4× 106.4× 10661.1× 101.1× 10661× 101× 10668× 108× 10556.6× 106.6× 10553.3× 103.3× 10555.65.6

11St St 8× 108× 10553× 103× 10551.5× 101.5× 10558× 108× 10443× 103× 10552× 102× 10553× 103× 10335.35.3

55th th 1.3× 101.3× 10559.2× 109.2× 10335.8× 105.8× 10332.4× 102.4× 10331× 101× 10449.4× 109.4× 10332.4× 102.4× 10335.25.2

88th th 3.6× 103.6× 10335.1× 105.1× 10223× 103× 10221.9× 101.9× 10228.5× 108.5× 10228.7× 108.7× 10222.3× 102.3× 10334.94.9

1111th th 3.6× 103.6× 10333.2× 103.2× 10222× 102× 1022--4.3× 104.3× 10222.4× 102.4× 1022--4.64.6

1515thth 3× 103× 10332x102x10331.1x101.1x1022--4.3x104.3x10225.1x105.1x1022--4.44.4

2020thth 4.4× 104.4× 10331.3x101.3x10331.1x101.1x1022--1.5× 101.5× 1022----4.24.2

2323thth 4.8× 104.8× 10331.3x101.3x1022----1.4x101.4x1022----44

2727thth 4.9× 104.9× 10331.3x101.3x1022----1.3x101.3x1022----44

3030thth 4.9× 104.9× 10331.3×101.3×1022----1.3x101.3x1022----44

- Initial Count 3 × 106

- Count during curdling 7 × 107

Table 2 : Effect of Mushroom on E . sakazaki growth during Table 2 : Effect of Mushroom on E . sakazaki growth during preparation andpreparation and storage of Damietta cheese at room storage of Damietta cheese at room temptemp . .

Table 3: Effect of Mushroom on E . sakazaki growth Table 3: Effect of Mushroom on E . sakazaki growth in Damietta cheese at refrigerator temp . in Damietta cheese at refrigerator temp .

StorageStorageTime/dayTime/dayControlControl

Fresh MushroomFresh MushroomPreserved mushroomPreserved mushroom

PHPH1%1%5%5%10%10%1%1%5%5%10%10%

Finished Finished cheesecheese3.6× 103.6× 10661× 101× 10668× 108× 10556× 106× 10552.2× 102.2× 10553.3× 103.3× 10551.7× 101.7× 10555.65.6

11St St 3× 103× 10551× 101× 10446× 106× 10333× 103× 10336.3× 106.3× 10553.9× 103.9× 10335.4× 105.4× 10335.55.5

55th th 1× 101× 10442.5× 102.5× 10331.6× 101.6× 10331× 101× 10332.5× 102.5× 10332× 102× 10331.8× 101.8× 10335.45.4

88th th 1× 101× 10338.5× 108.5× 10226.3× 106.3× 10226.2× 106.2× 10229.1× 109.1× 10222.4× 102.4× 10224× 104× 10225.35.3

1111th th 3.9× 103.9× 10331× 101× 10221.6× 101.6× 10336.3× 106.3× 10221.7× 101.7× 10221.2× 101.2× 10221× 101× 10225.25.2

1515thth 2.4× 102.4× 10334.1x104.1x10221.9x101.9x10221.2× 101.2× 10221.2x101.2x10331.1x101.1x10333.4× 103.4× 102255

2020thth 4.4× 104.4× 10334.6x104.6x10221 x101 x10331.9 x101.9 x10331.3× 101.3× 10331.2 x101.2 x10331.3 x101.3 x10334.94.9

2323thth 4.8× 104.8× 10331x101x10333 x103 x10332 x102 x10331.5x101.5x10331.5 x101.5 x10331.3 x101.3 x10334.84.8

2727thth 4.8× 104.8× 10331.8x101.8x10333.5x103.5x10332.2 x102.2 x10331.8x101.8x10332 x102 x10331.5 x101.5 x10334.64.6

3030thth 4.8× 104.8× 10331.9×101.9×1033 1.5x101.5x10331.6 x101.6 x10331.3x101.3x10332.2 x102.2 x10331.6 x101.6 x10334.64.6

- Initial Count 3 × 106

- Count during curdling 7 × 107