life technologies introduction: 6-dye evolution, prepare your lab for the future of ce fragment...

1 Life Technologies™ |

6 Dye Evolu+on : Prepare Your Lab for the Future of CE Fragment Analysis

Ann Allison Human Iden/fica/on Life Technologies


What is our Current Official Company Name?

A.  Applied Biosystems B.  Applied Biosystems part of Life Technologies C.  Life Technologies D.  I don’t care I am s/ll going to call you ABI no ma@er what


GROUNDED in Science INSPIRED by Scientists DRIVEN by Innovation

1 Company / 8 Trusted Brands / 600,000+ Citations


Quick Facts


Media Recognition

Ranked #9 in 2012

Ranked #15 in 2012

Ranked Among “The 50 Most Innovative

Companies in 2012”

318 Chip Awarded for Design & Engineering

In Embedded Technologies


Ion Semiconductor Sequencing Rapid, Benchtop Sequencing for All


Webinar hosted by Forensic Magazine Ion Torrent™ Semiconductor Sequencing for Forensic Applica9ons

Lisa Calandro Director of Product Management Life Technologies

Dr. Walther Parson Institute of Legal Medicine University of Innsbruck, Austria

Available to view “on-demand”: www1.gotomeeting.com/register/134635184


Improving Customer Experience Ini+a+ves


Customer Product Communication System

A web-‐based system that ensures the /mely delivery, and real-‐/me repor/ng, of important technical product informa/on to appointed contacts at each North America customer site. Launched in North America October 2011 §  Communica/on about cri/cal product quality issues

affec/ng specific HID customers: product re/rement, product discon/nua/on, new soJware version or patch release, user bulle/n, and non-‐cri/cal technical updates

§  Not a marke+ng channel! §  To sign up, go to:

h@p://marke/ng.appliedbiosystems.com/mk/get/ HID_Product_No/fica/on_Sign_Up


Is Your Lab Receiving Product No+fica+on Emails?

A.  Yes B.  No C.  Don’t know


HID Resource Hub - Coming Soon http://www.lifetechnologies.com/hid


ACTIVATE (Touch)

AIM (Autoscan)

RESULTS (COA info)

New Life Tech App – Technical Document App “Coming Soon” to an App Store near you

Sample -‐ Search for COA


www.lifetech.com/forensicnews


Supply Chain Improvements


Minor Modifications to Several AmpFlSTR® Kits §  Manufacturing of the AmpliTaq Gold® enzyme and 10X PCR Buffer II components transi9oning from Roche to Life Technologies −  Both components in the following kits: Profiler®, Profiler Plus®, Profiler Plus® ID, SGM Plus®,

COfiler®, Iden/filer®, Yfiler® & MiniFiler™ Kits

−  The buffer component only in the Iden/filer® Direct Kit (this kit already contains the AmpliTaq Gold® enzyme manufactured by Life Technologies)

−  Manufacturing of both components by Life Technologies will be conducted according to the same specifica+ons used previously by Roche.

−  All other kits already contain both the buffer and enzyme components manufactured by Life Technologies

§  Why are we doing it? −  To exercise greater control over the quality of key raw materials −  To prevent formula/on or QC issues which affect kit performance −  To be@er maintain reagent supply


Minor Modifications to Several AmpFlSTR® Kits

§  Our internal studies show the kits containing the components manufactured by Roche and Life Technologies to be interchangeable −  The buffer and enzyme components manufactured by Life Technologies

have already been tested rigorously during developmental valida/on of the Iden/filer® Plus, NGM™ and NGM SElect™ Kits

§  User Guides and/or Product Inserts for each of the affected kits will be updated to include our internal developmental valida/on studies demonstra/ng no substan/ve difference in performance compared to previous versions

§  A full revalida/on should not be necessary; however, laboratories should determine their own implementa/on requirements


Improving Workflow Efficiency


Time to Result

(hrs/per 24 samples) ~1-‐3.5 ~0-‐2 ~4 ~1.5 ~1

Maximized Efficiency for Single Source Sample Processing

Time to Result

(hrs/per 24 samples) 0 0 0.75 0.55 0.1

Total Time to Result = ~7-‐12 hrs

Collec/on Extrac/on Quan/ta/on/ Normaliza/on Amplifica/on Detec/on Analysis

Total Time to Result = ~2 hrs



Sample Prepara/on

Quan/ta/on/ Normaliza/on Amplifica/on Detec/on Analysis

Time to Result

(hrs/per 6-‐16 samples) 1-‐48 2 3.5 0.75 2-‐3 Time to Result

(hrs/per 13 samples) 1-‐3 2 1.5 0.55 1-‐2


Dependent on sample type and method

Improved Efficiency for Casework Samples


A short history of STR Multiplexing

Kit Name Release Date # of Loci # of Dyes*

AmpFlSTR® Blue 1996 3 2

AmpFlSTR® Profiler® 1997 10 4

AmpFlSTR® Identifiler® 2001 16 5

GlobalFiler™ 2012 24 6

*Includes size standard


What is the main benefit of 6-‐Dye chemistry?

A.  Increased discrimination power B.  Improved profile recovery from challenging samples C.  Improved genotyping accuracy D.  Increased concordance with legacy data (previous kits) E.  All of the above


Today’s Agenda

1:00 Introduc+on & Overview Ann Allison, Life Technologies

1:15 The GlobalFiler™ Kit: Going Further for Casework Jennifer Schroeder, Life Technologies

1:45 Next Genera+on Y-‐STR & Quan+fica+on Assay Development Updates Jill Muehling, Life Technologies

2:30 Break

2:45 Overview of 3500 and GeneMapper® ID-‐X Features and Func+onality Kellie Fenesan, Life Technologies

3:30 Moving to 6-‐Dye Analysis: Simplifying the Process Jennifer Schroeder, Life Technologies

4:00 Ques/ons and Answers

4:30 Mee/ng Concludes

Most of the presentations will be posted to SlideShare: http://www.slideshare.net/Lifetech_HID

life technologies introduction: 6-dye evolution, prepare your lab for the future of ce fragment...

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