macromolecules mystery lab day 2

WHO TOOK LESLIE ’S PHONE?

Macromolecules Mystery Lab Day 2

Day 2 Lab Safety

Chemical Safety Biuret indicator solution

Very dangerous to eyes – students MUST wear goggles Dangerous to skin – students must rinse skin for 15 minutes if contact

occurs Iodine indicator solution

Will stain skin – rinse thoroughly with water Will stain clothing – may not come out with washing Mildly dangerous to eyes – wear goggles

Glassware and general lab safety Report all spills to teacher immediately Report broken or chipped glassware immediately

Do not attempt to clean yourself Horseplay, unauthorized use of materials, and off task behavior

will not be tolerated.

Lab Jobs

Materials manager – gets the stuff, makes sure you have what you need, organizes equipment

Recorder – takes careful notes on procedure, observations (sight, sound), notes any errors, data

Group manager – reads the directions and helps keep the group on track, liaison with Beck

Dishwasher – ALL OF YOU!

Let me give you a quick tour of the room

Hypothesis

First, work with your team to complete the hypothesis for what substances these foods will test positive for

Pretzels

1. Crush pretzels with a mortar and pestle until a course powder is formed

2. Pour pretzel powder back into plastic container 1. DO NOT DO CHEMICAL TESTS IN THE MORTAR!

3. Perform a lipids test by rubbing some of the pretzel powder on brown paper

4. Add 2 mL (2 full dropper pipettes) of water to the pretzel powder

5. Perform a glucose test with the glucose test strip6. Perform a starch test by adding 5 drops of iodine7. Crush up more pretzels, add 2 mL of water, and perform

the protein test with Biuret solution. 1. DO NOT DO CHEMICAL TESTS IN THE MORTAR!

8. Record your results in the Day 2 data table.

Peanut Butter

1. Perform a lipids test by rubbing some of the peanut butter on brown paper

2. Add 2 mL (2 full dropper pipettes) of water to the peanut butter and stir to mix

3. Perform a glucose test with the glucose test strip

4. Perform a starch test by adding 5 drops of iodine

5. Get more peanut butter, add 2 mL of water, and perform the protein test with Biuret solution.


Jelly

1. Perform a lipids test by rubbing some of the jelly on brown paper

2. Add 2 mL (2 full dropper pipettes) of water to the jelly and stir to mix



5. Get more jelly, add 2 mL of water, and perform the protein test with Biuret solution.


Yogurt

1. Perform a lipids test by rubbing some of the yogurt on brown paper

2. Add 2 mL (2 full dropper pipettes) of water to the yogurt and stir to mix



5. Get more yogurt, add 2 mL of water, and perform the protein test with Biuret solution.


Beans

1. Mash beans with a mortar and pestle until a course mush is formed

2. Scrape bean mush back into plastic container 1. DO NOT DO CHEMICAL TESTS IN THE MORTAR!

3. Perform a lipids test by rubbing some of the bean mush on brown paper

4. Add 2 mL (2 full dropper pipettes) of water to the bean mush and stir to mix

5. Perform a glucose test with the glucose test strip6. Perform a starch test by adding 5 drops of iodine7. Smash up more beans, add 2 mL of water, and perform

the protein test with Biuret solution.1. DO NOT DO CHEMICAL TESTS IN THE MORTAR!


Dry evidence

1. Perform a lipids test by rubbing some of the dry evidence on brown paper

2. Add 2 mL (2 full dropper pipettes) of water to the dry evidence and stir to mix



5. Get more dry evidence, add 2 mL of water, and perform the protein test with Biuret solution.


Wet evidence

1. Perform a lipids test by rubbing some of the wet evidence on brown paper

2. Add 2 mL (2 full dropper pipettes) of water to the wet evidence and stir to mix



5. Get more wet evidence, add 2 mL of water, and perform the protein test with Biuret solution.


Record your data in the Day 2 table

Collaborate with other groups to complete the data table

Food

Carbohydrate Tests Protein Test Lipid Test

Test strip color

Glucose present

Iodine test color

Starch present

Biuret test color

Protein present

Lipid present

Pretzel (crumble into the container)

Butter

Jelly

Fat-free yogurt

Beans (mash into

a paste)

Dry evidence

Liquid evidence

Worker in break room Lunch he or she was eating

JoeAshleyBruceKiaraThief

Bean burrito with cheese

Fat-Free YogurtToast with peanut

butter and jellyPretzelsCombined liquid +

dry evidence

Who took Leslie’s new phone?

Conclusion

Work together to figure out who took Leslie’s new phone

Worker in break room

Lunch he or she was eating Glucose Starch Protein Lipid

Joe Bean burrito with cheese

Ashley Fat-Free Yogurt

Bruce Toast with butter and jelly

Kiara Pretzel

Thief Combined liquid + dry evidence

Conclusion

Worker in break

room

Did he/she take

Leslie’s new

phone?

How do you know? Describe the evidence that supports your conclusion.

Joe

Ashley

Bruce

Kiara

Make an evidence based claim for each person

Put in large trash can Clean/return

Food containers Brown paper bagWhite paperGlucose test strips

Wipe down and dry lab stations if needed

Return iodine to teacher

Clean stirring rods and dropper pipettes and return to teacher

Clean up

Your report needs

TitlePurposeHypothesis – 2 tables

Day 1 hypothesis Day 2 hypothesis

Results – 2 tables Day 1 test results Day 2 test results

Conclusion – 2 tables Table of who ate what Table with evidence based claims

Signature

macromolecules mystery lab day 2

Education