ori pvs1
DESCRIPTION
ori pRi. kan r. pTF101.1::Ri. 10.1 kbp. ori RK2 (incP ). RB. 2x P 35S. TEV enhancer. bar. ori pVS1. Tvsp. pTF101.1. 9.2 kbp. LB. aadA. pgl. ori colE1. ori RK2 (incP ). pE2759. 30.1 kbp. ori pSa (incW). pTF101.1::BIN19. pTF101.1::UCD2. tet r. 11.0 kbp. 13.0 kbp. - PowerPoint PPT PresentationTRANSCRIPT
ori pVS1pTF101.1
9.2 kbp
TEV enhancer
RB
Tvsp
LB
ori colE1
2x P35S
aadA
bar
ori pSa(incW)pTF101.1::UCD2
13.0 kbp
tetr
ori RK2(incP)
pTF101.1::BIN19
11.0 kbpkanr
ori pRipTF101.1::Ri
10.1 kbp
ori RK2(incP)pE2759
30.1 kbp
tetr
pgl
picASupplemental Figure 1. Schematic representation of the various T-DNA binary vectors and integration plasmid used in this study. The parent plasmid was pTF101.1. The region highlighted in blue indicates the T-DNA region plus the aadA gene incorporated into all other plasmids. LB/RB, T-DNA
left/right border regions; ori, vegetative origin of replication; 2xP35S, cauliflower mosaic virus double 35S promoter; TEV, tobacco etch virus; bar, phoshinothricin-resistance gene; Tvsp, vsp polyA addition signal; aadA, spectinomycin-resistance gene; tet/kanr, tetracycline/kanamycin resistance gene; pgl/picA, pgl/picA genes from the C58 chromosome.