ti MIDICI"A &IOLOGICA APRILI · GIUGNO 2004

M. De Bellis, N. Frasca

••.

TREATMENT OF WRINKLES AND SKIN SLACKENING USING THE INTRADERMAL INjECTION OF A COMPLEX HOMEOPATHIC REMEDY (MADE®) RESULTS OF A COHORT CLINICAL STUDY

ON 681 PATIENTS

SUMMARY

Wrinkles and skin slackening are the

most obvious slgn of the passíng of

time, i .e., 8geing from a mere biotogicat

viewpoint. However, they also reflect

the psycho·neuro-endocrino-immuno­

loglcal (PNEI) vlsion of the human being:

in otller words. the skin is tlle main tar­

get of one's psychological experiences

during the somato-psychic process,

whereas it is the starting polnt of or­

ganic wounds that will eventually be­

come the "soul scar" during the psycho­

somatic proce·ss .

The beauty ot the face has been atways

conneeted with a smooth, glowing and

young skin. Therefore, In order to exor­

cise ageing, our society makes us turn

to Plastie Surgery 01' Aesthetic Medi­

cine, whieh are not often eompletely

sueeessful or do not satisfy the pa­

tients ' requlrem..nts fully.

For more than five years, the homeo­

pathle remedy MAOe" (Guna, Milan), has

been an etfective alternative to con­

ventional pharmaeologieal treatment

and can eertalnly be regarded as a ref­

erenee drug In Aesthetic Medicine. The

cohort study hereunder, earried out be­

tween 1998 and 2003 on 681 patients.

has proved the efficacy and good toler­

ability of MAOe- both in preventive and

therapeutle terms, in the homeo­

mesotherapie treatment of skin slaek­

enlng as well as all types of wrlnkles,

espeeially linear perioeular and perlt ­

abial wrlnkles , showing the best re·

sults in patients aged between 30-40

years and 40-50 years.

KEY WORDS

SKIN AGEING, WRINKLES, MAOE0,

HOMOTOXICOLOGY

A "'E~ICINA &10 OGICA APRllE· GIUGNO 2004

INTRODUCTION

The most significant and obvious sign of

the transilíon from youth to old age is

the appearance of facial wrinkles.

" Beauty" has always been associated

with having young, smooth and glowing

skin . However, apart from external

beauty, the skin also reflects a person's

troubles, anxiety and pain - it is a mir­

ror of the sou l and every w rinkle tells

the story of that person 's experiences in

life .

The skin is the pattern on which the

psycho-neuro-endocrino-immunologi­

cal (PNEI) vision of Medicine is based

and where the psychosomatic signs of

psychological troubles become clearly

visible over the years The skin is also

the starting point for the organic wound

that wi II eventua Ily become the "sou I

scar" (non-acceptance of the se lf) du­

ring the somato-psychic process.

On this basis, we can understand why

nowadays, regardless of one's level of

education, social class or religion , it has

become so important to "exorcise" the

aging process, sta rting with trying to eli­

minate the problem of w rinkles.

According to the American Society of

Plastic Surgeons

(wwwplasticsurgeryorgl), blepharo­

plasty and fa ce lifts account for Just a

third of the total number of plastic sur­

gery operations requested by Ameri­

canso Even in the non-surgical sector of

Aesthetic Medicine, figures are sti ll im­

pressive Collagen Implants, Hyaluronlc

Acid fillers, Goretex implants, Botulinic

Toxin, mechanical dermal abrasion and

C02 laser skin resurfacing are still the

most common therapeutlc stronghold

for specia I ists .

However, results do not always meet the

patients ' high expectations.

For more than five years, the homeopa­

thic remedy MAOE ® (GUNA, Milan),

has been an effective alternative to con­

venti onal pharmacological treatment

In this article, the biological interpreta­

tion of the etiopathogenesis of sk in

aging and wrinkles will be on the basis

of the correct therapeutic rationale for

these problems: by studying the com­

position of MAOE®, 'vve wlll be able to

identify this rationale in its homeophar­

macological structure .

The results of an observation study, car­

ried out between 1998 and 2003 on

681 male and female patients, into the

efficacy of MAOE® in the treatment of

wrinkles and slackening of the face and

neck tissues , will be examined and de­

scribed later on.

SKIN AGING Chronological aging of the skin is the re­

sult of a mixture of biological, bioche­

mical and molecular events established

by the genetic code of each individual

(chronoaging). This is why not all peo­

pie grow old in the same way and the

skin is not the same in al l mdividuals.

Other environmental chemical and

physical factors contribute to aging, and

these are of varying importance in de­

termining ilS type and severity (photoa­

ging)

In order to fully understand the path o­

genic mechanisms leading to aging of

the skin, we need to analyse and con­

sider the same mechanisms that lead to

general organic aging and examine the

metabol ic and structural characteristlcs

of human skin.

We a Iso need to consider that chronoa ­

ging and photoaging cou ld have a 51­

gnificant impact on the alteration ofso­

me physiological cutaneous mecha­

nisms, not only as independent events

but also as synergic factors .

The results of sk m aging are clearly vi­

sible in 1055 of elasticity and turgidity,

and the appearance of wrinkles It can

be traced back to a slowdown in ce ll

turnover (SYCOTIZATION Process ac­

cording to c lassic Homeopa thy)

with a subsequent reduction in elastic

ti ssue and 1055 of the support structure

(but not j ust the support structure as

maintained by Pi schi nger and Heine)

represented by the subcutaneous, loose

fibrillar connective tissue (dermis) .

SKIN PHYSIOPATHOLOGY AND THE ROLE OF SUBCUTAI\IEOUS COI\lI\lECTIVE TlSSUE

The normal physiological process of

chronoaging affects a II the structures of

the tegumental system: at epidermis le­

vel , one can see a reduction in mitoses,

a tendency towards premature keratini­

sati on. the dispersion of Melanocytes,

and a reduction in Langerhans cells.

The basal membrane shows a progres­

sive smoothing with the disappearance

of the epithelial crests and dermal pa­

pillae

The dermi s shows a 1055 of thickness

and thinning out of the vascular sup­

port: the co llagen fibres are fragmented;

the elastic fibres are disorganised; the

interstitial substance tends to become

un iform , and there are lower numbers

o f fibroblasts , mastocytes and Langer­

hans cells.

But wha t causes these phenomena?

There are two ma i n theones:

1) accord i ng to the first theory ("pro­

grammatic H ) , the programming of

cel l death lieswithin the geneti c co­

de;

2) according to the second theory ("de­

generative H

), aging is a process that

is dependent on exogenous factors

(especia Ily photoaging for the ski n)

and endogenous factors (horm ona l

and immuno logical factors, for

example) that synergically cause a

series of metabolic failures (Oepo­

si tion Phase according to the Table

of Homotoxicosis) and alterations in

molecular syntheses. You need only

to think about w hat happens in par ­

ticular areas of the face, such as the

eyelids and some periocular areas ­

here a reduction in collagen type I

synthesis is clearly linked to age, but

ultraviolet radiation , and the subse ­

quent produ ction of free radical s,

causes a drastic reduction in elastin

and collagen storage and this pro­

bably affects post-transcription me­

chanisms.

WRINKLES

Wrinkles are the most visible evidence

of cutaneous atrophy and are caused by

damage to the collagen and elastic Fi­

bres.

We must remember that, as the skin

does not have its own muscular struc­

tures, it is the contraction oF the muscles

below it that determines its shape. As ti­

me goes by, due to changes in tone and

elasticity, the skin can no longer relax

and the First marks remain etched on the

skin and gradually get worse.

Wrinkles can be divided into the Follo­

wing categories:

Linear wrinkles: these are linked to

facial expressions; at first they are re­

versible and are more common in

women . They mainly appear around

the eyes (crow 's Feet) , between the

eyes (from Frowning). around the

mouth (vertically on the upper lip or

around the mouth, due to smoking

For example), horizonta lIy on the Fo­

rehead (related to emotions, in par­

ticular to anxiety);

Glyphic wrinkles: these are related

to a greater accentuation oF the or­

dinary cutaneous structure. They ap­

pear on cheeks in particular ;

Creases these are caused by pro­

longed Facial expressions (for in­

stance while sleeping) ;

Wrinkles between the nose and

mouth : these are deep incisures ap­

pearing between the external edge

oF the mouth and the nose wings,

delimited by muscles (mouth orbi­

cular and buccinator musc les).

HOMOTOXICOLOGICAL INTER­PRETATION OF THE ETIOPATHO­GEr'lIESIS OF WRINKLES AND SKIN SLACKENING

According to homotoxicological

physiopathology, wrinkles can be cate­

gorised under Deposition Phase - Im­

pregnation of the Tegumental System

.rll I mil . From a homotoxicological

point-oF-view, it is apparent how wrin­

kles and the slackening oF Face and neck

tissues are caused by changes in the

MATRIX (in Fact, both the Deposition

Phase and the Impregnation Phase are

part of the "Matrix Interstitial Substance

Phases").

The connective tissue has mistakenly

been regarded aSJust a support tissue;

the dermis, which has always been re­

garded as just the tissue on which the

skin lays.

However, Homotoxicology regards the

matrix as a truly specialised organic

structure, the "Basic Regulation

System": all internal and external chan­

ges to our environment aFFect cell me­

chanisms via the interstitial substance.

It is via the matrix that the cells are able

to communicate with the external en­

vironment - the amount oF inFormation

sto red in the matrix and passed on to

cells as instructions on their physiolo­

gical functioning is enormous. The ma­

trix is the place where the neurovege­

tative endings unravel and the psycho­

lA loIIOICIN4 510l001e. APRILI· GIUGNO 2004

brillar connective tissue are in the der­

mis, then a subsequent pathological al­

teration will obviously appear.

In fac!. it is essential to keep the dermis

well-drained and metabolically eFfi­

cient in order to keep the skin looking

young .

The dermis is composed oF:

Fibroblasts and Fibrocytes and their

extracellular metabolites

Collagen fibres and elastic Fibres

Glycosaminoglycans (GAGs) and

proteoglycans (PGs)

Blood vessels and nerves

Immunocompetent cells

There are two diFFerent areas in the der­

mis:

1. the papillary dermis, which is su­

perFicial and thin and located near

HOMOTOXICOLOGY SIX-PHASE TABLE

Simplified table

fll'Uttl

allergle

_di D"","~lo.n.

-1­ ----.

neuro-endocrino-immune informatlon

is conveyed through the neural and en­

docrine substances and cytokines.

We know that correct cell Function ing is

based upon the anatomical and Func­

tional integrity oF the matrix , and u lti ­

mately on its "cleanliness" and its de­

toxiFication levels. An accumulation of

stress factors at this level can lead to a

potential triggering oF a pathological

process. If these changes in the loose fi­

the dermatoepidermalJunction, and

is rich in matrix but poor in collagen

and elastin ;

2. the reticular dermis, a thicker area

located between the papillary der ­

mis and the subcutaneous adipose

tissue: it is rich in collagen and ela­

stic Fibres, contains lower quantities

oF matrix, and is well vascularised

(blood and Iymph capillaries affe­

rent from the underlying subcuta­

~ MEDIWIA II0lO(;I" APRILE· GIUGNO 2004

neous adipose tissue) .

The action of the homeopathic drug

MAOE~ is targeted on these two struc­

tures ,

The etiopathogenetic process that leads

to the destructuring of the derm is and ,

therefore, to wrinkles is characterized

by a series of connected events 11 111 r l i: - Phase 1: a reduced supply of 02 and

nUlrients to the dermis cel!s caused by

the pol!ution of lhe matrix due to cala­

bol ites produced in cel! turnovers (chro­

noaging) and by undrained toxins (free

radical photoaging) , causes a slowdown

of intracellular metabolisms and sub­

sequent enzymatic damage;

- Phase 2: the meta bol ie d istress of the

fibroblast affects its activity leading to

a drastic reduetion in the incretion of

lhe matrix components (in particular

Hyaluronic acid) and the col!agen and

elastic fibres;

- Phase 3: the connective tissue weave

loses compactness, The lack of glycosa­

minoglycans has a dramatic effect on

skin hydration (Hyaluronic acid is a

highly hydrophilic molecule) and ilS tur­

gidity; the reduced vascularisalion of

the epidermis causes lhe skin to lose its

brightness and normal Iymphatic drai­

nage is slowed down, resulting in a vi­

cious cycle that is difficult to break .

Chronoaging + Photoaging

Enzymatlc damage

Slowdown in cell metabolism

Cell alteration

Tissue destructuring (wrinkle)

wrinkles are primarily a METABOLlC

AlTERATION (a result of suffering aged

cel!s)

Instead , a wrinkle trealment should be

an integrated therapy w here fibroblasts

can begin their synthesizing activily (af­

ter specific organ preparation stimula­

lion) as their proper meta bol ic function

has returned as a result of the action of

the coenzyme substrates of the Krebs

cycle and they, therefore, have sufficienl

energy levels to maintain their

neosynthesis activity.

At lhe same time, lhe integrity of lhe

functlonal structure of the dermis

should be maintained by means of con­

tinuous detoxification and drainage,

The homeopathic drug MADE® acts on

lhis "cascade" via the synergic aetion of

its four nuclei of components

IPI( n rwsl ,1

7, INTERMEDIATE CATAlYSTS

(Vitamin C 06, Vitamin B7 06, Vi­

tamin 86 06, Nicotinamid 06, Ac.

Cis aconitum 06, Ac, Fumaricum

06, Ac Alpha-ketoglucaricum 06,

Baryum oxalsuccinicum 06, Na­

trium oxalaceticum 06, Natrium

pyruvicum 08, Magnesium gluco­

nicum 06, Manganum phosphori­

cum 07);

[' B I

If any wrinkle trealmenl is to be reliable

then it must take al! these pathogenic

processes into consideralion and not

just act on one aspect of them .

A therapy that is simply a means of

compensating for a deficiency in Hya­

luroni e acid from chronoaging or in

other eomponents of the dermal matrix ,

would nollake into accounl the fact that

2, "SUIS" ORGANOTHERAPIES

(Co/lagen suis o8-o30-Funiculus

umbilicalis suis oJO-030-Cutis suis

08-030, Placenta suis 070, Mu­

sculus suis 020, Hepar suis O JO, Glandula suprarenalis suis O JO),

3, CLASSIC HOMEOPATHIC REMEDIES

(Sulphur 072, Mercurius solubilis

Hahnemanni 020, Calcium fluora­

tum 030, Galium aparine 06, Thu­

ja 06),

4, HOMEOPATHIZED ENZYMES

(Hyaluronidase 08-030)

Through its own components, each o{

the drug's {our nuclei develops a struc­

tural and functional tropism that is spe­

cific to each o{ the steps in the process

o{ the etiopathogenic cascade o{ wrin­kles PI' T' IRrr: ~ . - -,:

1 - THE NUCLEUS OF INTERMEDIA TE CATALYSTS

Its eleelive target is the milochondrion

and, in particular, the Krebs eycle. It has

a release action on the mechanisms as­

signed to energetic metabolism, via the

enzymatic stimulation induced by ho­

meopathic dilutions

The intermediate calalysts in MADE ®

are therefore vital, as wilhout the relea­

se of the oxidative phosphorylation pro­

cesses and the cells ' renewed produc­

tion of energy, lhe fibroblasts could not

reacl to the prol iferalive trophic stimu­

lation simultaneously induced by the

"Suis" organotherapies.

The core of the nucleus of catalysls is

_-ketoglutaric aCld, a Krebs eyele sub­

strate that acls on the enzyme, _-keto­

glularic-dehydrogenase, which is often

blocked in the initial phases (stil! rever­

sible) of fibroblast degeneration,

Homeopathized vitamins are, of cour­

se, included for their antioxidanl activity

(aclion against photoaging and protec­

tion of the matrix glycosaminoglycans),

Vitamin C was included as il ís an im­

portant cofaetor in the transformation of

Prol i ne into Hydroxyprol ine,

The two oligo elements (Magnesium

gluconicum 06, Manganum phospho­

ricum 010) are particularly important

for their catalytic action on col!agen

metabolism.

aparine 06; ThU¡a 06

"-.1. .l.'-J~.

Inhibirion ef the deslrueluríng

ae/ion 01 aulologou

Trophie ae/ion on skill

and eonneetive

Anlioxidant and eatalylie ae/ion on eollagen

metabollsm

lA MEDICINA !IOLOGICA APRILE· GIUGNO 2004

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2 - THE NUCLEUS OF "SU/S" ORGANOTHERAP/ES

In accordance with the observations of

Dr. H . H. Reckeweg, "Organotherapy "

with homeopathized organ preparations

is based on the use of pigs as donors.

From a homeopathic point of view, we

can confirm that a pig organ or homeo­

pathized pig tissue is extremely similar

to the human homologous organ and as

a result of this "similarity" (greater than

that of other animal species), the thera­

peutic efficacy of a homeopathized

preparation is even greater

This likeness is particularly apparent at

immunological level.

The result is the marked organotropism

of the " Suis" protein for the human ho­

mologous protein.

Due to pigs' almost completely ineffec­

tive detoxification systems. their tissues

and, therefore. their proteins are parti­

cularly toxic (steatosis degeneration).

One, therefare, crea tes a protein struc­

ture that has the patential characteristics

af a nasade, plus the specific properties

af arganotropism.

A "Suis" organotherapeutic homeopa­

thic preparation is an organ-specific no­

sode, that, via a subliminal immunolo­

gical mechallism, triggers the immune

response of not only the entire RES

Target sites

of MADE®

components

WAINKLES

CLASSIC HOMEOPHATY REMEDIES Sulphur 012: Mercurius sol Hahn 020. Calcium fluoralum 030; Gallum

•

aval! ,ronirlJ>=

Therapeutic strategy

'ORAININ-G DRUGS....",. ..... ' .r­ -'­ ,~J'

MADE ~

'INTCRMEfiIATE CATALYSTS ANO ÓUGOElEMENTs. '.Vil. C 06/ Vil. 81 06/ Vil. B6 06/ Nlc01inamid 06 / Ac. Cis.' ;-aéOríll. 06/ Ar:. lumaricum 06/ Ac. A-keloglul. 05/ Barlum ,oxalsucc. 06/ NatriulTI oxalac. 06/ Natrium pyruv. 08/

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LA MEOICINA aIO~OGIC_ APRILE - GIUGNO 2004

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Some examples ot tratment with MACEe. Left side: befare treatment; right side: after treatment.

system (hystiocytic macrophagicJ, but

also of the target organ or tissue. We can

confirm that the use of organotherapies

in treatment causes a localized "mi­

croinflammation" , which although, of

course, not clinical (because of the di­

lution), is sufficient to "waken" the func­

tionality of the connective matrix.

Their action is also partly "trophic": the­

se Suis proteins, or some substances

contained in them, are substrates for the

enzyme reactions of protein synthesis

("codifying matrices") The fact that they

are diluted in accordance with the Laws

of enzyme kinetics, makes them per­

form as inductors, speeding up protein

synthesis

The following are of particular interest:

• Collagen suis

Although Collagen suis triggers, with

the immunological mechanism, the

function of the loose fibri llar connecti ­

ve tissue of the dermis via a subclmical

inflammatory type process, it also car­

ries out a trophic action by stimulating

the fibroblast to increte autologous col­

lagen.

Therefore, we cannot refer to Coll agen

suis simply as having a supplementa­

tion, and, therefore plastic action, but

real biostimulation.

• Funiculus Umbilicalis suis

It is well-known that this organ prepa­

ration is extremely rich in glycosaml­

noglycans (especially Hyaluronic acid).

When these substances are homeopa­

thically diluted, according to enzyme

kinetics Laws, they act as mductors,

starters, and codifying matrices for the

synthesis of autologous glycosami­

noglycans.

• Musculus suis and Cutis suis

It is easy to guess at the rationale behind

the action of these remedies - they sti­

mulate the function of their respective

targets, performing an anti-degenerati ­

ve action and stimulating their tro­

phism.

• Placenta Suis

Some of the Growth Factors contained

in the placenta are of particular interest

- especially FGF (Fibroblast Growth

Factor) that can stimulate the fibroblast

receptors to activate their metabolism;

and EGF (Epidermal Growth Factor), a

polypeptide that acts on the epidermal

metabolism.

Placenta derivatives are also well ­

known for their action on microcircula­

tion.

• Hepar Suis

The inclusion of this organotherapy was

necessary for activating the emunctory

drainage of this organ, which is closely

I inked to the skin, both from an energe­

tic (Traditional Chinese Medicine) and

an ontogenetic point of view.

• Glandula suprarenalis Suis

According to Homotoxicology, the sti­

mulation of the suprarenal gland is the

basis for the treatment of pathologies

that are degenerative or somehow con­

nected with ageing.

3 - THE NUCLEUS OF CLASSIC HOMEOPATHIC REMEDIES

The main homeopathic polycrests with

recognised anti-degenerative properties

were selected, such as Galium aparine

(essential detoxifying and cleavage ac­

tion on toxins acting on connective tis­

sue metabolism) and Thuja (main re­

medy against dysmetabolic mesenchy­

mal pathologies which are the source of

the pathogenesis of wrinkles)

Sulphur is the most su itable remedy for

the skin (the skin contains large

amounts of Cysteine, a sulphur amino

acid (-SH) The sulphur enzymes are ex­

tremely important for the proper func­

tioning of the tegumental system. Sul­

phur also has an important toxin cen­

trifugation action and, therefore, a drai­

nage action as well

Calcium fluoratum and Mercurius so­

lubilis Hahnemanni act on areas prone

to wrinkles - typical of the "Fluoric"

Homeopathic constitution - where we

can see the "wearing out" of the con­

nective tissue leading to varicose veins,

ptoses and wrinkles. In its pathogenesls,

lA MEDICINA !IOlOGICA APRILE· GIUGNO 2004

Mercurius solubilis Hahnemanni is cha­

racterized by signs of destruction.

4 - THE HOMEOPATHIC ENZYME NUCLEUS

The inclusion of Homeopathic Hyalu­

ronidase is MAOE" s real innovation.

The 08 and 030 homeopathic dilutions

of this enzyme regulate and limit the

physiological destructuring activity of

the autologous hyaluronidase. As a re­

sult, the interstitial substance is com­

pacted and the wrinkle is reduced.

It is essential to act on the Hyaluronic

acid as a whole and "protect" it as, to all

intents and purposes, it can be regarded

as the true "conductor" of the connec­

tive matrix structure and function - it

packs the main macromolecular com­

ponents of the dermis around itself,

such as collagen, proteoglycans, fibro­

nectin and fibropectins, acting as the

framework.

This remedy is, therefore, a true thera­

peutic unit whose structure provides the

correct homotoxicological strategy for

treating degenerative skin diseases

In order to apply an effective wrinkle

treatmen!, we should assume that wrin­

kles are a metabolic alteration. We can

then understand the therapeutic impor­

tance of the nucleus of intermediate ca­

talysts, the importance of including

"suis" organotherapies and homeopa­

thized Hyaluronidase and why classic

homeopathic remed ies are effective:

.. The physlological metabollc activity

of the fibroblast is re-establ ished as a re­

sult of the enzymatic release promoted

by these substances and this is a funda­

mental condition for the dermis cells to

be able to respond to the stimulus in­

duced by the suis organotherapies via

the neosynthesis of the components of

the Interstitial Substance. The modula­

tion of the degenerative process, con­

trolled by the classic homeopathic re­

medies in the preparation, slows down

the a Iteration of the cOllnective stroma,

assisted by the homeopathized hyalu­

ronidase which, by reducing the activity

Il MEDICIt.¡f, 510l0GIC~ APRILE· GIUGNO 2004

PHASE4 TAEATMENT OFAAEAS PAONETOTHE APPEAAANCE OFWAINKLES

CONNECTlVE TlSSUE DAAINAGE

Conslltul/on dra/nage and support

Epidermis

Popillory dermis

Corium

Tendinous dermis

Hypodermic vascular plexus

Hypodermis

Subcutaneous cell tissue

¡~~oJl/dant and ca/a/ytic ' •1_ sellon on /he collageñl-.

.. :-", 'metabol/sm;·N .:1, ./ ~, . . .::

Prolilmtive lrophlc and anllgenetlll/vesI/mula/ron on ltu!

skillilnd lhe CO/Jnsctivs T lissul!

Nerve ond ~ubpopillory vmol plexus

Superficial nervQUS plexus

Fibrous eones

Adipose lobules

Superficíollayer

Subcutoneous vessels and nerves

Aponeurosis

of the corresponding enzyme, fosters

the slowing down of the degenerative

process and, all things considered, en­

courages the compacting of the dermis.

BIOSTIMULATlON OF THE SKIN. MATERIALS AI\lD IVIETHODS

Both the mesotherapy needle (4 mm.

30G) and the collagen needle (13 mm­

30G, Flf 1. :\ ) can be used for this pro­

cedure. The mesotherapy needle is used

for making classlc intradermal wheals in

accordance with the mesotherapy me­

thod, injecting 0.2-0.3 mi for each

wheal IPIr II IH hlll.

- The collagen needle is used for can­

nulating the wrinkle by moving the

needle gently from left to right while in­

jecting the contents of the syringe

IPKn RI 71.

We recommend treating the whole af­

fected area possibly some acupuncture

points:

LI 18 (Large I ntesti ne 18): on the an­

terior margin of the sternocleidoma­

stoideus, on a level with the upper

margin of the thyroid cartilage.

ST 4 (Stomach 4): about 1 cm late­

rally to the corner of the mouth.

ST 5 (Stomach 5) on the vertical li­

ne of the pupil, below the inferior

margin of the orbit.

TH 23 (Triple Heater 23) superio­

rally and posteriorally to the extre­

mity of the eyebrow.

GB 1 (Gall bladder 1) 1 cm laterally

to the external margin of the orbit.

SI 19 (Sma 11 Intestine 19) anterior to

the auricular tragus.

The treatment genera lIy consists of 7-10

treatments carried out once a week,

followed by one or more maintenance

sessions which can be monthly, bi- or

trl-monthly

lA !\¡DI'IN~ arO~OGICA APRILE· GIUGNO 2004

With homeo-mesotherapic biostimula­

tion, one can see an overall revitali­zation, a considerable improvement in

tissue tone and a clear easing of the

wrinkles.

The improvement is gradual and, above

all, lasting. The end result of the therapy

is a relaxed and rested face with toned,

glowing skin.

MAOE' can be used both as a treatment

for reducing the signs of aging and as a

preventive treatment for maintaining a

youthful face.

As a result of its specific homeopathic

preparation, the medicine has no col­

lateral effects or contraindications. No

biocompatibility test is therefore nee­

ded

PATIENTS ANO METHOOS

In this study, we evaluated the effecti ­

veness of the homeopathic complex re­

medy MADE® in the treatment of wrin­

kles and skin slackening via a series of

subjective and objectlve clinical indi­

cators.

It was an observation multicentric study,

carried out according to the Godd Cli ­

nical Practice Rules.

For this study we included 681 patients

of both sexes (578 female - 103 male)

aged between 35 and 75 (female) and

between 40 and 70 (male), divided in­

to d ifferent age ranges.

AII patients attend i ng the practices of

the doctors taking part in this study ha­

ve been included, without exclusion

criteria.

The period of the study lasted 5 years

from 1998 to 2003

The treatment consisted of 8 sessions on

a weekly basis was carried out. For 50­

me patients the treatment was conti ­

nued on the basis of one session a

month after the end of the treatment and

another every 2-3 months.

3.8% of the patients dropped out of the

treatment after the first few sessions, for

reasons that were not dependent on the

programme.

.. THE TUNNELLlNG TECHNIQUE

It is a method involving the décollement of thtissue triggering a small inflammatory reaction and leading to the disappearance of wrinkles.

The method applied involved making li­

near infiltrations, 1 cm apart, which we­

re parallel to the skin surface in the me­

dium and medium-deep dermis, accor­

ding to the mesotherapic technique, or

making infiltrations inside the wrinkles,

according to the tunnelling technique.

1I I!. 71

The resu Its were eva I uated before and

after the specific treatment via the sub­

jective classiflcation of the visual and

tactile characteristics of the wrinkles

and the slackening of the face and neck

tissues II \I! . 'l .

Mild reactions to the treatment were ob­

served in 20 cases (3%) with slight ery­

thema in the inJection site, which dis­

appeared of its own accord after a few

minutes.

DISCUSSION - CONCLUSIONS

This observation multicentric study has

shown that MAOE® is highly effective

and has high levels of tolerability in the

homeo-mesotherapic treatment of all

types of wrinkles and skin relaxation,

especia Ily linear periocu lar and peri la­

bial wrinkles and the revitalisation of

the face and the neck - in Groups A and

B, in particular, there was a considera­

ble reduction in the compromisation of

these 2 areas of facial skin, with the dis­

appearance ofwrinkles; in Groups C, O

and E there was a steady improvement,

with the compromisation of the areas

progressing from being obvious to slight

{groups A rnr.u :\ ¡ I(.l R] " H. D) and B

II'\AII 1IIIe I RI:'" 1(1 1111\1lll-1I ); Groups

C l' \BLl:i (lIGLlH .~ 12. ni T\Bl( ti!. 0 1(,1, DU{ilfll..LRCS II F.lT\I:IU IUI and E Ir\

111 F 7 (I·lt,1 J{rs 1 h. 17\1

The best resu Its were observed in fema­

le patients between 30-40 and 40-50

years old (groups A and B): this is par­

ticularly significant as it corresponds to

the results one would expect from a bio­

stimulating therapy, which regards a

young person/adult responder as an in­

dividual who still has an optimum reac­

tion capacity both from a metabolic and

a histologic point of view, and with a

state of connective tissue that has not

yet been compromised

The drug's performance was extremely

good both for the patients (Iow cost, vi ­

sible and lasting results, satisfied the re­

quest for biological therapies) and for

the doctors (absence of any risk, ap­

preciable results).

~This study has proved the practicabi­

lity, tolerability and, above all, the effi­

cacy of MAOE@both in preventive and

therapeutic terms. and it can therefore

be regarded as a reference drug in Ae­

sthetic Medicine. •

II MIOICI~~A 810 OGltA APRllf" GIUGNO 2004

Before ~

Alter

o "0--

Absenl Slighl Obvious

FEMALE PATIENTS - Group A (30-40 years) Perilabial area

% 100 90 80 70 60 50 40 30 20 10 O

Compromised face ski n

I \R I

Group B

(female patients ­

40-50 years old),

before (B) and after

(A) therapy; n = 96

8 A

85 90 Absent (89) (94)

6 3 Slight (6) (3)

5 3Obvious

(5) (3)

FEMALE PATIENTS - GROUP 8 (40-50 years) Perilabial area

% 100 90 80 70 60 50 40 30 20 10 O

Absenl Slighl Obvious Compromised face ski n

FEMALE PATIENTS - Group A (30-40 years) Periocular area

% 100 90 80 70

50 40 30 20 10 O

-- ­

60 ---1 ---'~-i

6 1--

Absenl Slighl Obvious Compromised face skin

8 A 8 A B A 8 A

65 73 28 58 61 64 49 50 (68) (76) (29) (60) (64) (67) (51) (52)

19 15 45 23 28 26 34 35 (20) (16) (47) (24) (29) (27) (35) (36)

12 8 23 15 7 6 13 11 (12) (8) (24) (16) (7) (6) (14) (12)

FEMALE PATIENTS - GROUP 8 (40-50 years) Periocular area

% 100 90 - --- Before 80 Alter 70 60 50 ~

40 30 24 4 20 10 O ---'

Absenl Slighl Obvious Compromised face ski n

B A B

133 146 48 75 7 Absent (71) (78) (26) (40) (4)

35 27 110 95 126 Slight (18) (14) (58) (51) (67)

20 15 30 18 55Obvious

(11 ) (8) (16) (9) (29)

FEMALE PATlENTS - GROUP e (50-60 years) Perilabial area

% 100 90 Before­80 After ~ 70 60 50 40 30 20 -_--­10 O

Absent Slight Obvious Compromised face skin

--------------------~

------,,....:::.....---l --~

16-­

LA MEDICLNA BIOLOGICA APRILE · GIUGNO 2004

'·\B .1

Group e (female patients ­

50-60 years old),

before (B) and after

(DA) therapy; n =

A

10

(5)

133 (71)

45

(23)

100 90

80 70

B A B A 188

2 1 O 1

(1) (1 ) (O) (1)

99 103 99 102 (53) (54) (53) (54)

87 84 89 85 (46) (45) (47) (45)

FEMALE PATIENTS - GROUP e (50-60 years) Periocular area

~-----------------------

--------------------~---67 71

60 --------------1 50 -------1 40 ---~---I 30 ----- ­ --1 20 ----- ­ ----1 10 .. ., O ----'

Absent Slight Obvious Compromised face skin

r.-'\n ti

Group D

(female patients ­

60-70 years old),

before (B) and after

(A) therapy; n = 116

Absent 19

(16) 27

(23) O

(O) O

(O) o

(O) o

(O) 1

(1)

1 (1 )

o (O)

o (O)

Slight 52

(45) 45

(39) 55

(48) 66

(57) 52

(45) 66

(57) 57

(49) 59

(51) 57

(49) 62

(53)

Obvious 45

(39)

44

(38) 61

(52) 50

(43) 64

(55)

50 (43)

58

(50)

56 (48)

59

(51) 54

(47)

FEMALE PATIENTS - GROUP D (60-70 years) Perilabial area Periocular area

% 100 % 100 90 Before----------­ 90 Before- ­80 - 80

FEMALE PATIENTS - GROUP D (60-70 years)

After After 70 70 ­

6050 ___60 40 4550 40 30 30 20 ---- ­ 20

10 - ­lO o o O

4t! I

O - o-"~ Absent Slight Obvious Absent Slight Obvious

Compromised face skin Compromised face ski n

h~( 5

LA MIDI[IN~ BIOlOGICA APRllI · GIUGNO 2D04

B A B A B A B A B A

1 \ II .,

Group e (female patients ­

50-60 years old),

before (B) and after

(DA) therapy; n = 188

Absent 133 (71)

146 (78)

48

(26)

75

(40) 7

(4)

10 (5)

2 (1)

1 (1)

O (O)

1 (1)

SlIght 35

(1 8) 27

(14) 110 (58)

95 (51)

126 (67)

133 (71 )

99 (53)

103 (54)

99 (53)

102 (54)

Obvious 20 (11)

15 (8)

30 (16)

18 (9)

55 (29)

45 (23)

87 (46)

84 (4$)

89 (47)

85 (45)

FEMALE PATlENTS - GROUP e (50-60 years) .... FEMALE PATIENTS - GROUP e (50-60 years)

% 100 90 00 70 60 50

4030 20

10 O

Perilabial area % 100

I L Before;- 90

ª le ~58 -~

51 40

--26~ 1 - ~, , ~. ,-"~, .•,, . t-, '. 16

_· ... 1· . o'> ! 9 --1 ~-:< -- tl, -.­

- -'- .~

Absent Slight Obvious Compromised tace skin

B A B A

19 27 O O Absent (16) (23) (O) (O)

SlIght 52

(45) 45

(39) 55

(48) 66

(57)

Obvious 45 (39)

44 (38)

61 (52)

50 (43)

FE MAL E PATIENTS - GROUP D (60-70 years) Perilablal area

% 100 90 Befare- ­80 - Alter 70 60 50 40 30 20 10 -- O OO

Absent Slight Obvious Compromised tace ski n

5

- 00 70 60 50

4030 20

'" 10 --4­O

Absent

I_. _______~~_._----""-

B A B A

O O 1 1 (O) (O) (1) (1)

52 66 57 59 (45) (57) (49) (51 )

64 50 58 56 (55) (43) (50) (48)

Periocular area

Slight Obvious Compromised tace ski n

f-\ll b

Group o (female patients ­

60-70 years old),

before (B) and after

(A) therapy; n =116

O (O)

O (O)

57 (49)

62 (53)

59 (51)

54 (47)

~. FEMALE PATIENTS· GROUP D (60-70 years) Periocular area

% 100 90 Betare'--' ------------ ­80 - Alter70 ­60 -----­50 -----45 -43- ­40

;g ---..===----J-. 10 --0--0- ­O

Absent Slight Obvious Compromised tace skin

l A MEDICINA ¡IO LOeIC

r \B ,

Group E

(female patients - >

70 years old),

before (B) and after

(A) therapy; n =103

APRILE · GIUGNO 2004

B

3 Absent

(3)

49 Slight (48)

51Obvious

(49)

FEMALE PATIENTS - Group E (>70 years) Perllablal area

% 100 90 Before. 80 ­ ~--

Alter _..

70 ­60 50 40 30 20 }10 O O O

Absent Slight Compromised lace skin

1\11. H

Group B

(male patients ­

40-50 years old),

before (B) and after

(A) therapy; n =50

Slight

Obvious

BibJió ra h 1. De Bellis M. - Manuale di Omeomesoterap ia -

Guna Ed , Mi lano; 2003 .

2. Dipartimento Scientilico Guna - Omotoss ico lo ­

gia in Medicina Estetica "Appunti di Bio-mesote­

rapia" ; Guna Ed. , Milano; 1998.

3. Dipartimento Scientilico Guna - Quaderm di Cli­

nica Omotossicologica "11 Drenaggio"- Guna Ed.,

Mi lano: 2000.

4. Di Pietro A. . Di Sante G. - II recupero dell'elastici­

ta e del turgore cutaneo mediante iniezione intra­

dermica di acido ialuronico (Ial-Sistem") con tec­

% 100 90 ­

80 l 70 ­60 50 40

Obvious

A B A

3

(3)

O

(O)

O

(O)

56 (54)

47

(46) 53

(51 )

44

(43)

56

(54)

50

(49)

30 20 10 O

B A B A B A

O O O O O O

(O) (O) (O) (O) (O) (O)

47 51 47 48 44 44

(46) (49) (46) (47) (43) (43)

56 52 56 55 59 59 (54) (51 ) (54) (53) (57) (57)

FEMALE PATIENS - GROUP E (>70 years) Periocular ares

Before

After

O

Absent Slight Obvious Compromised lace skin

Absent

B

40

(SO)

7

(14)

3

(6)

A B A

43 38 41

(S6) (76) (82)

5 7 5

(10) (14) (10)

2 5 4

(4) (1 0) (S)

nica cross-linked - Estralto da Giornale Italiano di

Dermatologia e Venereologia vol. 136-N .3/187-194

(Giugno 2001 ) . Edizioni Minerva Medica - Torino

5 Duprat H. - Materia Medica Omeopatica - Fratell i

Palombl Edltori . Roma; 1983.

6. Reckeweg H.H. - Materia Medica Omeopatica -

Guna Ed., Milano; 1990.

7 Stevens A. , Lowe J. - Istologia Umana ­

Casa Editrice Ambrosiana. Milano; 2003.

8. Seutemann S .. Kastner R. - Omeopatia con I blo­

cataliz zatori - Guna Ed.. Milano ; 1991 .

B A B A B A

lS

(36) 21

(42)

38

(76)

40

(80) 36

(72) 39

(78)

22

(44)

20

(40)

10

(20)

10

(20)

12

(24)

10

(20)

10

(20)

9

(1S)

2

(4)

O (O)

2

(4) 1

(2)

LA ~I,DICIN_ ~1¡)LOGiC, APRIL¡ · GIUGNO 2004

1 ·\1~ 1

Group C

(ma1e patients ­

50-60 years old),

before (B) and after

(A) therapy; n = 43

B A B A B A B A B A

25 25 22 25 15 15 24 26 24 24 Absent (59) (59) (51) (58) (35) (35) (56) (60) (56) (56)

15 16 14 12 20 21 16 14 16 17 Slight (34) (37) (33) (28) (46) (49) (37) (33) (37) (39)

3 2 7 6 8 7 3 3 3 2Obvious (7) (4) (16) (14) (19) (16) (7) (7) (7) (5)

fAB. 1U

Group O

(male patients ­

60-70 years old),

before (B) and after

(A) therapy; n = 10

B A B A B A B A B A

2 2 2 3 1 1 1 1 3 3 Absent (20) (20) (20) (30) (10) (10) (10) (10) (30) (30)

5 6 5 5 6 7 5 6 5 6 Slight (50) (60) (50) (50) (60) (70) (50) (60) (50) (60)

3 2 3 3 3 2 4 3 2 1Obvious

(30) (20) (30) (30) (30) (20) (40) (30) (20) (10)

Doctor's O(O) [1 0(0) O (O) 211 (31) 470 (69)evaluation

I i ---t :, lr DE BELLlS M. - Terapia delle rughe e Patlent's I

O(O) O(O) O (O) 191 (28) 490 (72) del rilassamento cutaneo con inie­evaluatlon zioni intradermiche di un farmaco

omeopatico complesso (MADE") ­11.1) II Global evaluation on the treatment : results

Risultati di uno studio multicentr ico

su 681 pazienti.

La Med . Biol. 2004/2; 7-19.

-_-~liIiMDm:tIf! -' Doctor's Dr_ Massimo De BellisO(O) O(O) O(O) 7 (1) 674 (99)evaluation - Specialista in

Idroclimatologia Medica Patient's O(O) O(O) O(O) 20 (3) 661 (97)

I Via Cesare da Sesto, 15evaluation I - 20123 Milano

r \f: Global evaluation on tolerability