01-fermentasi
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Agroindustri ProdukFermentasi
Teknologi Industri Pertanian
Fakultas Teknologi Pertanian
Universitas Brawijaya
PokokBahasan(1)
Sumber Karbon, Nitrogen, Mineral,Vitamin dan Faktor Tumbuh, Prekursor
Media Fermentasi4
Perhitungan Mikroba, ModelPertumbuhan, Pertumbuhan Mikroba,Kultur Fed-Batch dan Kontinyu
KinetikaPertumbuhan
3
Bakteri, Khamir, J amur, Metode Isolasi,Isolasi Mikroorganisme, PerbaikanStrain, PenyimpananKultur
MikroorganismedalamIndustri
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Pengertian fermentasi, ProdukFermentasi, Sejarah Fermentasi, Industrifermentasi masa kini
Pengertianfermentasi
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Sub Pokok BahasanPokok BahasanPertemuan
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Cara Pembuatan, Tahapan Proses,Mikrobiologi danBiokimia Tape, Ragi Tape, BremPadat
Tape8
Proses Fermentasi, Cara Pembuatan,Perubahan Mikrobia danBiokimia
Kombucha7
Mikrobiologis Inokulum, MekanismePembentukan, Faktor Berpengaruh, TahapanPembuatan, SifatFisikokimia,Keragaman Proses Pembuatan
Tempe6
Faktor Abiotik dan BiotikFaktor Lingkungan5
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PokokBahasan(3)
Fermentasi AsamAsetat, FermentasiAsamLaktat
Fermentasi AsamAsetatdan Laktat
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KecapKedelai, KecapAmpas Tahu,Kecap Ikan
Fermentasi Kecap11
Susu, ProdukFermentasi Susu, ProdukSusu Asam, Manfaat Produk SusuFermentasi
ProdukFermentasiBerbasis Susu
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Proses Pembuatan, KeragamanMikrobia
Nata DeCoco9
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Kultur dan Pemeliharaannya,Mekanisme Fermentasi,Komposisi Campuran,Penanganan Biji danPenggilingan, Kualitas BahanBaku, Peralatan danBahan baku,Produksi Alkohol dari Tetes
Fermentasi Alkohol13 - 14
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Pustaka
Dania, W.A.P., N. Hidayatdan I. Nurika. 2006. MembuatMinuman Prebiotik dan Probiotik. Trubus Agrisarana.Surabaya
Hidayat, N., M.C. Padaga dan S. Suhartini, 2006, MikrobiologiIndustri. ANDi Offset. Yogyakarta
Hidayat, N; Wignyanto; I Nurika danS Suhartini. 2006.Agroindustri ProdukFermentasi. TIP-FTP-UB. Malang
Steinkraus, K.H. (ed), 1989, Industrialization of IndigenousFermented Foods, Marcel Dekker, Inc. New York.
Ulrich, K.T. and S.D. Eppinger, PerancangandanPengembangan produk (terjemahan), PenerbitSalembateknika, J akarta.
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FERMENTATION
Agroindustri Produk Fermentasi
TIP – FTP – UB
Fermentation
Derived from ‘fervere’: boiling appearance of the action of yeaston extracts of fruit/malted grain.
It is due to the production of CO2 bubbles caused by theanaerobic catabolism of the sugars present in the extract.
Biochemists =>fermentation =generation of energy bycatabolism of organic compound
Industrial microbiologist =>fermentation =any process for theproduction of product by the mass culture of a microorganism(Stanbury& Whitaker 1984)
Fermentation =gradual change done by enzymes of microorganism (mold, yeast, bacteria) (Hidayatet al 2006)
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The range of fermentation processes
There are 4 major groups of commercially importantfermentations :1. produce microbial cells (or biomass) as the product, co : baker’s
yeast, yeast for single cell protein, ‘ragi’, probiotics2. produce microbial enzymes, co: amylase, protease, pectinase,
catalase, glucose oxidase, etc3. produce microbial metabolites, co : ethanol, citric acid, vitamins,
acetone, butanol, glutamic acid, lysine, etc4. modify a compound which is added to the fermentation– the
transformation processes, co: conversion of ethanol to aceticacid at vinegar, production of steroid, antibiotics andprostaglandin
the advantages : operating at relatively low temperature, withoutthe requirement for potentially polluting heavy metal catalyst.
The Chronological Development of TheFermentation Industry Pre 1900 : alcohol and vinegar; batch, using pure cultures and
‘good vinegar’ 1900 - 1940 : baker’s yeast, glycerol, citric acid, lactic acid,
acetone / butanol; bath & fed batch - using pure cultures 1940 - date : penicillin, streptomycin & other antibiotics,
gibberelin, amino acid, nucleotides, enzyme, transformation;batch, fed batch, continuous, mutation and selectionprogrammes essential
1960 - date : single cell protein; continuous & medium recycle,
genetic engineering of production strains 1979 - date : foreign compounds, not normally produced by
microbial cells ex : insulin, interferon; batch, fed batch &continuous batch, genetic engineering to introduce foreigngenes into microbial host
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Fermentation products and their microbialproducers
Saccharomyces cerevisiae
Lactic acid bacteria
Aspergillus oryzae, Zygosaccharomyces rouxii
Rhizopus oryzae
Hansenula, Saccharomyces
Acetobacter aceti
Aspergillus niger
Lactobacillus delbrueckii
Acetobacter xylinum
Lactic acid bacteria
Corynebacterium glutamicum
Brevibacterium lactofermentum
Penicillium chrysogenumNeurospora sitophila
Monascus purpureus
Bakery, beer, wine
Yoghurt, kefir, probiotics
Soy sauce
Tempeh
‘Tapai’
Acetic acid
Citric acid
Lactic acid
Nata Nata
Pickles, sauerkraut
Glutamic acid
Lysine
Penicillin‘Oncom’
Angkak
MicrobesProducts
The present development of industrialfermentation
Microbial cell of probiotics=> capsule, drink/beverages
Amylase and glucose isomerase for fructose syrupproduction as diet sweetener
Colouringagentfrom microorganism for textile colours
Biodieselas energy source to replace petroleum
Bioinsecticides
Microbial bioplastics(Polyhydroxyalkanoates) Isoflavonof soybean
Lipase for detergent
Lactic acid for PLA (Polylacticacid)