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2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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2018 CBEES-BBS TOKYO, JAPAN
CONFERENCE ABSTRACT
2018 8th International Conference on Bioscience,
Biochemistry and Bioinformatics (ICBBB 2018)
January 18-20, 2018
Global Front, Surugadai Campus, Meiji University, Tokyo,
Japan
Sponsored by
Published and Indexed by
http://www.icbbb.org/
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Table of Contents
2018 CBEES-BBS Tokyo, Japan Conference Introduction 3
Presentation Instruction 4
Keynote Speaker Introduction 5
Brief Schedule for Conference 18
Detailed Schedule for Conference 20
Parallel Sessions Index 22
Schedule of Sessions 24
Poster Session 79
Conference Venue 91
One Day Visit and Tour 93
Note 94
Feedback Information 97
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2018 CBEES-BBS Tokyo, Japan Conference
Introduction
Welcome to 2018 8th International Conference on Bioscience, Biochemistry and Bioinformatics (ICBBB 2018) which is sponsored by Hong Kong Chemical, Biological & Environmental Engineering Society (CBEES) and Biology and Bioinformatics (BBS). ICBBB conference series held annually to provide an interactive forum for presentation and discussion on Bioscience, Biochemistry and Bioinformatics. The conference welcomes participants from all over the world who are interested in developing professional ties to and/or exploring career opportunities in the region. The conference should serve as an ideal forum to establish relationships from within Japan and other regions of the world.
Papers will be published in one of the following conference proceedings or journal:
International Conference Proceedings Series by ACM. Archived in the
ACM Digital Library, and indexed by Ei Compendex and submitted to be
reviewed by Scopus and Thomson Reuters Conference Proceedings Citation
Index (ISI Web of Science).
International Journal of Bioscience, Biochemistry and Bioinformatics
(IJBBB, ISSN: 2010-3638). Included in the Engineering & Technology
Digital Library, and indexed by WorldCat, Google Scholar,Cross ref,
ProQuest.
Conference website and email: http://www.icbbb.org/; [email protected]
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Presentation Instruction
Instruction for Oral Presentation
Devices Provided by the Conference Organizer:
Laptop Computer (MS Windows Operating System with MS PowerPoint and Adobe Acrobat
Reader)
Digital Projectors and Screen
Laser Stick
Materials Provided by the Presenters:
PowerPoint or PDF Files (Files should be copied to the Conference laptop at the beginning of
each Session.)
Duration of each Presentation (Tentatively):
Keynote Speech: 25 Minutes of Presentation and 5 Minutes of Question and Answer
Plenary Speech: 25 Minutes of Presentation and 5 Minutes of Question and Answer
Invited Speech: 15 Minutes of Presentation and 5 Minutes of Question and Answer
Regular Oral Presentation: 12 Minutes of Presentation and 3 Minutes of Question and Answer
Instruction for Poster Presentation
Materials Provided by the Conference Organizer:
The place to put poster
Materials Provided by the Presenters:
Home-made Posters
Maximum poster size is A1
Load Capacity: Holds up to 0.5 kg
Best Presentation Award One Best Oral Presentation will be selected from each presentation session, and the
Certificate for Best Oral Presentation will be awarded at the end of each session on January
18, 2018 and January 19, 2018.
Two Best Poster Presentations will be selected from poster session, and the Certificate for
Best Poster Presentation will be awarded at the dinner banquet on January 19, 2018.
Dress Code Please wear formal clothes or national representative of clothing.
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Keynote Speaker Introduction
Keynote Speaker I
Prof. Kenta Nakai
The University of Tokyo, Japan
Kenta Nakai was born in Osaka, Japan, in 1963. He received the PhD degree on the prediction
of subcellular localization sites of proteins from Kyoto University, Kyoto, Japan, in 1992.
From 1989, he has worked at several institutions, including, Kyoto University, National
Institute of Basic Biology, and Osaka University. From 1999 to 2003, he was an Associate
Professor at the Human Genome Center, the Institute of Medical Science, the University of
Tokyo, Japan. Since 2003, he has been a full Professor at the same institute. He was elected as
the president of the Japanese Society for Bioinformatics in 2006 and 2007. His main research
interest is to develop computational ways for interpreting biological information, especially
that of transcriptional regulation, from genome sequence data. Two of his papers reporting the
development of a knowledge-based prediction system of subcellular localization sites have
been cited more than 1,200 times so far.
Topic: “Analyses of Transcriptional Regulatory Codes through a Variety of NGS Data”
Abstract—Nowadays, it is becoming more and more important to interpret the impact of
differences between individual genomes and/or the genomes of normal and abnormal cells
(e.g., tumor cells). In many cases, however, this is difficult mainly because we do not
understand how the gene regulatory information is encoded in the non-coding regions of the
genome. To overcome this difficulty, so-called next generation sequencers (NGSs) are quite
useful: they are not only useful just for reading DNA sequences per se but also getting a
variety of information, such as the information on gene expression profiles in a single cell
under various conditions/stages. They are also useful for probing the epigenetic status and the
approximate 3D structure of chromatin. In this talk, I will introduce some of our research
activities in this direction. The talk will include (1) our efforts in automatically extracting the
frequent patterns of motif placements in the genome and our future plan of constructing an
encyclopedia of gene regulatory regions; (2) our ongoing project to combine multiple sources
of Hi-C data for understanding the differential gene expression through differential chromatin
loop structure; and (3) our efforts in analyzing single-cell transcriptome data to understand
inter-tissue communication in the mammalian immune system.
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Keynote Speaker II
Prof. Sung Wing Kin, Ken
National University of Singapore, Singapore
Prof. Dr. Wing-Kin Sung received both the B.Sc. and the Ph.D. degree in the Department of
Computer Science from the University of Hong Kong in 1993, 1998, respectively. He is a
professor in the Department of Computer Science, School of Computing, NUS. Also, he is a
senior group leader in Genome Institute of Singapore. He has over 20 years experience in
Algorithm and Bioinformatics research. He also teaches courses on bioinformatics for both
undergraduate and postgraduate. He was conferred the 2003 FIT paper award (Japan), the
2006 National Science Award (Singapore), and the 2008 Young Researcher Award (NUS) for
his research contribution in algorithm and bioinformatics.
Topic: “Fast, Sensitive and Accurate Detection of Virus Integrations in Cancer”
Abstract—The study of virus integrations in human genome is important since virus
integrations were shown to be associated with diseases. In the literature, few methods have
been proposed that predict virus integrations using next generation sequencing datasets.
Although they work, they are slow and are not very sensitive.
This talk introduces a new method BatVI to predict viral integrations. Our method uses a fast
screening method to filter out chimeric reads containing possible viral integrations. Next,
sensitive alignments of these candidate chimeric reads are called by BLAST. Chimeric reads
that are co-localized in the human genome are clustered. Finally, by assembling the chimeric
reads in each cluster, high confident virus integration sites are extracted.
Finally, we applied BatVI to some cancer datasets and we will discuss the findings we have.
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Keynote Speaker III
Prof. Manoj R. Tarambale
Marathwada Mitra Mandal’s College of Engineering, Pune, India
Prof. Manoj R. Tarambale has received Bachelor's Degree (B.E.) in Electrical Engineering
from BVCOE, Pune-43, University of Pune, India, in 1992 and Master of Engineering Degree
(M.E.) in Control System ( specialization in Instrumentation ) from WCOE, Sangli, Shivaji
University, Kolhapur, India in 2002. Currently, he is pursuing his Ph.D. degree from
PACIFIC University, Udaipur, India in the field of Biomedical Engineering. He has one year
industrial experience and twenty one years teaching experience. At present, he is working as a
Head of Electrical Engineering Department of Marathwada Mitra Mandal’s College of
Engineering, University of Pune, Pune-52, India.
He has published fifteen papers in prestigious International Journals and in International
Conferences. He has got “Most Excellent Paper Award” and “Inter Science Scholastic Young
Investigator Award” for his technical papers published. His main research interests are in the
field of Bio-Medical Image Processing, Bio-Medical Instrumentation, Bio-sensors, Green
Environment, Computer Applications in Bio-Medical Analysis, Artificial Intelligence in
Disease Detection and Electrical Engineering. He is giving an important contribution in
implementing various early detection cancer technique projects through Bio-Medical Image
Processing. He has also done two consultation projects for the reputed industries. Prof.
Tarambale is a member of Institute of Engineers (India) and Indian Society for Technical
Education (ISTE).
Topic: “Detection of Medical Disease (Lung Cancer) Using Image Processing Tool–An
Engineering Approach”
Abstract—Today, cancer is one of the most formidable health problem faced by mankind.
This cancer confirmation process is complex, time consuming and costly. It might be possible
during the above process the stage of the cancer may change. Though MRI, CT scan, PET etc.
radiological images helps us to detect lung nodule easily, but because of huge cost involve
still avoided in poor and developing country. Inexpensive method involve detection of cancer
from simple chest X-ray. Abnormal masses seen in the chest X-ray in the form of white spot
/ tumors are analyze through engineering image processing methods, so that in early stage
cancer conformation took place, which reduce the mortality rate. In an engineering approach
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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to detect lung cancer various steps involved are data acquisition, image processing,
segmentation, feature extraction and use of artificial intelligence. First digitized images are
obtain by scanning X-ray image by high resolution scanners and adjusted to standard size by
applying size normalization algorithm. The image processing refer to the tasks necessary for
enhancing the quality of acquired digitizes X-ray scanned images. . The quality of the original
image obtained can be improved further by applying histogram equalization algorithm and
segmentation of suspicious region is done by using various edge detection algorithms,
labeling algorithm etc. Various features are extracted on the basis of mathematics using
simple software programming which help to classify suspicious tumor as malignant or benign
using machine learning process or also called it as using Artificial Intelligence. The proposed
system will not replace the doctor’s role in detection of cancer but it will help doctor to take
correct decision in short time with accuracy (It will act as second opinion before conformation
of cancer).
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Keynote Speaker IV
Prof. Satoru Miyano
The University of Tokyo, Japan
Satoru Miyano, PhD, is the Director of Human Genome Center, the Institute of Medical
Science, the University of Tokyo. He received the B.S. (1977), M.S. (1979) and PhD (1984),
all in Mathematics from Kyushu University, Japan. He is an ISCB Fellow. His research
mission is to develop "Computational Medical Systems Biology towards Genomic
Personalized Medicine, in particular, cancer research and clinical sequence informatics. He
has been involved as PI with the International Cancer Genome Consortium, the Grant-in-Aid
for Scientific Research on Innovative Areas (MEXT) "Systems Cancer Research in
Neo-dimension", and MEXT Priority Issues on Post-K computer "Integrated Computational
Life Science to Support Personalized and Preventive Medicine". By massive data analysis and
simulation with the supercomputers, his group is developing computational methods to link
differences in our genomes to diseases, drugs, and environmental factors with systems
understanding.
Topic: “Unraveling Cancer Systems Disorders from Big Data by Supercomputers”
Abstract—Cancer is a very complex disease that occurs from accumulation of multiple
genetic and epigenetic changes in individuals who carry different genetic backgrounds and
have suffered from distinct carcinogen exposures. We present our computational methods for
breaking cancer big data by using the supercomputers SHIROKANE at Human Genome
Center of University of Tokyo and K computer at RIKEN Advanced Institute of
Computational Science. The first challenge is unraveling gene networks and their diversity
lying over genetic variations, mutations, environments and diseases from gene expression
profiles of cancer cells. The second part of this lecture gives details of a suite of
bioinformatics tools named Genomon for analyzing cancer genomes and RNA sequencing
data produced by next-generation sequencers. We present some of our recent results on cancer
genomics with Genomon, including a mechanism involved with “how cancer evades immune
systems.
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Keynote Speaker V
Prof. Chan Jin Park
Incheon National University, South Korea
Prof. Chan Jin Park graduated from Korea University, and got Master and PhD degrees in
same university. His major fields of research are the air pollution control, greenhouse gas
technology and odor management technology. His another interests is green growth policy.
He is now full-professor in Incheon National University at Urban and Environmental
Engineering School. He is member of INU ensemble taking part in piano (Chamber orchestra
of his University).
Topic: “Development of Air Quality Management in Urban Cities”
Abstract—As the economic growth increases various air pollutants are generated to
atmosphere gradually until the air pollution become severe especially in urban areas.
Transportation and industrial facilities and power plants are main reasons of urban air
pollution in major cities of asian regions. Trans-boundary air pollutants such as fine
particulates and odors have been especially common challenge to comfortable and healthful
life of human being. In these situations clean air policy using clean fuel and new and
renewable energy are clearly the main solution to make sustainable air quality. In this research
current states of air quality in major cities were analyzed and the various emission sources
and policies of air quality managements were estimated. Effective methods of air quality
control in urban were suggested to make more clean cities in urban areas.
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Keynote Speaker VI
Prof. Harold Yih-Chi Tan
National Taiwan University, Taiwan
Employment History:
2011~, Director, Center for Weather Climate and Disaster Research
2007-2009 Director, Disaster Research Center, National Taiwan University
2003-2009 Director, Hydrotech Research Institute, National Taiwan University
2003/2-2003/5-Visiting Scholar, Civil and Environmental Engineering, UCLA
1994-now Professor, Bioenvironmental Systems Eng., National Taiwan University
Professional Career Highlights:
1. Professor and Director, National Taiwan University
2. Vice Chairman- ICID- Working Grouping of Environmental Impact of Irrigation, Drainage
and Flood Control
3. National Correspondents of the IAHS Association, IUGG- Chinese Taipei Committee
4. Secretary General, ICID-Chinese Taipei Committee
5. Chairman, Taiwan association of Engineering and Environment
Prof. Harold Yih-Chi Tan's researches related to earthquake to analyze the groundwater level
changed with analytical solution and observed data to be verified to published at Water
Resources Research. Furthermore, the national policy of watershed management and Flood
mitigation after the 921 Chi-Chi Earthquake in Taiwan is published on Natural Hazards. His
research has focused on climate change, land subsidence and disasters reduction.
Topic: “University Capacity Building to Help Local Government Setup Resilient Community
in Taiwan”
Abstract—Global warming impacts of climate and environment changes and it increased
frequency of extremely weather. It causes not only high frequency of disaster, but raising the
high intensity of hazards. For examples, Taiwan 921 earthquake in 1999, South Asia tsunami
in 2004, Hurricane Katrina in 2005, United State of Indiana hail stone in 2006, China Sichuan
earthquake in 2008, Typhoon Morakot at Taiwan in 2009, Haiti earthquake in 2010, Tohoku
earthquake and tsunami in 2011, Eastern Australia in 2012, Typhoon Haiyan at Philippines in
2013, and Bosnia, Serbia and Croatia encountered the most serious floods in past 120 years in
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2014. South Asia floods in 2015. Jiuzhaigou earthquake and typhoon Hato damages threated
Macau and Hong Kong in 2017. Hurricane Harvey is a catastrophe in American in 2017. The
local government is concerning more for damages brought by disasters in Taiwan. The
capacity and manpower of local government is not enough to implement full disaster
prevention in communities level without the help from outside resources. Universities of
Taiwan contribute professional technology, manpower and knowledge to support local
government to raise disaster management capacity in three stages, including risks
identification, adaptation and mitigation, and emergency response.
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Plenary Speaker I
Assoc. Prof. Hiroyuki KUDO
Meiji University, Japan
Prof. Dr. Hiroyuki Kudo received both the M.E. and Ph.D. degrees in the Department of
Electronics and Communications from Waseda University in 1999 and 2004, respectively. He
was a research officer of MEMS laboratory, Tokyo Metropolitan Industrial Technology
Research Institute from 2003 to 2007. He worked at Tokyo Medical and Dental University as
an assistant professor from 2005, a junior associate professor from 2007 to 2011 and an
associate professor from 2011 to 2013. Currently, he has been an associate professor of
Department of Electronics at Meiji University. Currently, his research interests include
biomicrosystems based on enzymatic biosensors and immunosensors and for life science
applications.
Topic: “Electrochemical Biosensors for Healthcare IoT”
Abstract—The internet of things (IoT), which is a new paradigm rapidly spreading in highly
developed information society, have been expected to open new window in treatment and
diagnosis in medicine and daily personal healthcare. Particularly, plenty of physical
information (e.g. body weight, temperature, fat rate, etc.) has been already utilized in cloud
services. However, hurdles for using biochemical information in such information services are
still high. One of the most considerable reason is that simplified means for obtaining
biochemical information are not provided. From this point of view, we position that simplified
method to measure or assess biochemical information (e.g. blood content, saliva content, etc.)
as the key technology for providing health big data and healthcare IoT. It is necessary to
realize a series of processes from ‘sampling’ to ‘measurement’ by an operation that anyone
can do with simple and low-cost instruments for the applications in those fields. We have been
developed new biosensors and biomicorsystems for those purposes by combining the
microelectromechanical system (MEMS) techniques and biochnology. In this talk, our recent
status of development and possible applications will be presented.
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Plenary Speaker II
Prof. Keimei Oh
Akita Prefectual University, Japan
Dr. Keimei Oh was born in Shanghai, China. He received B.Sc. in the Department of
Chemistry from Shanghai University and Ph.D. degree from the Graduate School of
Agricultural and Life Sciences, The University of Tokyo in 1997. After working at RIKEN as
a Special Postdoctoral Fellow, he joined the Department of Biotechnology faculty at Akita
Prefectural University in 1999. In 2003, he worked as a visiting scientist at US Department of
Energy, Plant Research Laboratory in Michigan State University. He was appointed as
Associate Professor at Akita Prefectural University in 2007. Currently, he is working in the
field of design and synthesis biological active chemicals targeting plant hormone biosynthesis
and signaling transduction pathways. He received numerous awards including the Society
Award of the Japanese Society for Chemical Regulation of Plants.
Topic: “Chemical Genetics Strategy Identifies Small Molecules Induce Triple Response in
Arabidopsis”
Abstract—To explore small molecules with ethylene like biological activity, we conducted a
triple response based assay system for chemical library screening. Among 9600 compounds,
we found that N-[ (1, 3, 5-trimethyl-1H-pyrazol-4-yl) methyl]-N-methyl-2-
naphthalenesulfonamide (EH-1) displayed promising biological activity on inducing triple
response in Arabidopsis seedlings. Chemical synthesis and SAR analysis of EH-1 analogues
with different substitution on the phenyl ring structure of the sulfonamide group indicated that
3, 4-dichloro-N-methyl-N-(1, 3, 5-trimethyl-1H-pyrazol-4-yl-methyl) benzenesulfonamide
(compound 8) exhibits the most potent biological activity. To determine the action mechanism,
we conducted RNA-Seq analysis of the effect of EH-1 and ACC, the precursor of ethylene
biosynthesis, following the quantitative RT-PCR confirmation. Data obtained from RNA-Seq
analysis indicated that EH-1 and ACC significantly (above 20 fold of control) induced the
expression of 39 and 48 genes, respectively. Among which 5 genes are up-regulated by EH-1
as well as by ACC. We also found 67 and 32 genes are significantly down-regulated,
respectively. Among which 7 genes are in common. For quantitative RT-PCR analysis. 12
up-regulated genes were selected from the data obtained from RNA-Seq analysis. We found a
good correlation of quantitative RT-PCR analysis and RNA-Seq analysis. Based on these
results, we conclude that the action mechanism of EH-1 on inducing triple response in
Arabidopsis is different from that of ACC.
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Plenary Speaker III
Assoc. Prof. Riichi Kajiwara
Meiji University, Japan
Riichi Kajiwara is Associate Professor of Department of Electronics and Bioinformatics at
Meiji University. He received a B.Eng. from Tohoku University in 1993, and M.S. and Ph.D.
degree in information science from Tohoku University, in 1995 and 1998. During the Ph.D.
course degree, he was working at Electrotechnical Lab AIST in Tsukuba science city, and
learned about the basis of electrophysiology and the optical imaging technique with the use of
voltage-sensitive dye. From 1998 to 2013 he worked at AIST, eventually as a Senior Research
Scientist. His research interest is the brain network physiology of the mnemonic and
emotional function.
Topic: “Network Properties of Limbic Neurons Revealed by Voltage-Sensitive Dye Imaging”
Abstract—The 'limbic system' is considered as a crucial structure for the neural plasticity
caused by learning and memory behavior. Therefore the neural circuitry of limbic system is
the primary target of functional modifications relating to the higher cognitive dysfunctions
caused by various factors. The in vitro brain slice preparations have been used for the purpose
so often, due to the difficulty in recording neural activities from deep brain structures under in
vivo conditions. Particularly, hippocampal slice preparations are more commonly used to
investigate the plasticity on a cellular / synaptic level. However, the functional connectivity
should also be examined in much larger scales. Here we discuss about this issue by showing
the optical imaging data obtained from two types of ex vivo brain preparations,
cortico-hippocampal brain slices and isolated whole brain preparations. The isolated whole
brain preparation, in which multi-synaptic circuits and the intracellular activity they generate
are well preserved, can be useful to examine such large scale neural circuitry. In the
presentation, we describe about the functional connectivity of various brain regions in the
limbic system using this unique preparation. This experimental approach combines the
advantages of the in vivo experimental condition with those of in vitro slice preparations, i.e.
an intact synaptic network, excellent mechanical stability, and control over the ionic and
biochemical extracellular environment. In particular, it provides easy access to brain areas of
the limbic system and preserved the neuronal network of the entorhinal-hippocampal loop.
Here we present example data obtained from this preparation in combination with optical
imaging of voltage-sensitive dyes.
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Invited Speaker I
Assoc. Prof. Siew Woh Choo
Xi'an Jiatong-Liverpool University, China
Choo completed his PhD study in Genetics from the University of Cambridge and secured a
scholarship from the Singapore government, to pursued MSc at the School of Medicine at the
National University of Singapore. He is currently an Associate Professor at Xi'an
Jiatong-Liverpool University. Choo has 15 years of working experience in research, academia
and private industry in the fields of genetics and bioinformatics, and mastered a number of
high-end core technologies. He has led a team to participate in local and international projects
with a total funding amount of about RMB 10 millions and published more than 53
peer-reviewed articles (1st or corresonding author in at least 42 articles) in prestigious
journals including Nature and Genome Research with >4850 citations. He has set up and led
the International Pangolin Research Consortium that has members from prestigious Yale
University, Peking University, Washington University, St. Petersburg State University and
Smithsonian Conservation Biology Institute. Choo has also actively transformed scientific
research achievements by setting up a high-tech enterprise and won several national awards.
He has been the Chairman and CEO of a biotechnology company, as well as a Senior
Scientist and Consultant at a personal genomics company. He has served on the editorial
board of Scientitic Reports and has been appointed as National Bioinformatics Expert
Appraiser Panel of the National Malaysian Qualifications Agency (MQA). In addition, he
devoted himself to the higher education industry, taught and educated many postgraduates,
postdoc, undergraduates, and research assistants.
Topic: “Pangolin Genomes and the Evolution of Mammalian Scales and Immunity”
Abstract—Pangolins, unique mammals with scales over most of their body, no teeth, poor
vision, and an acute olfactory system, comprise the only placental order (Pholidota) without a
whole-genome map. To investigate pangolin biology and evolution, we developed genome
assemblies of the Malayan (Manis javanica) and Chinese (M. pentadactyla) pangolins.
Strikingly, we found that interferon epsilon (IFNE), exclusively expressed in epithelial cells
and important in skin and mucosal immunity, is pseudogenized in all African and Asian
pangolin species that we examined, perhaps impacting resistance to infection. We propose that
scale development was an innovation that provided protection against injuries or stress and
reduced pangolin vulnerability to infection. Further evidence of specialized adaptations was
evident from positively selected genes involving immunity-related pathways, inflammation,
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energy storage and metabolism, muscular and nervous systems, and scale/hair development.
Olfactory receptor gene families are significantly expanded in pangolins, reflecting their
well-developed olfaction system. This study provides insights into mammalian adaptation and
functional diversification, new research tools and questions, and perhaps a new natural
IFNE-deficient animal model for studying mammalian immunity.
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Brief Schedule for Conference
Day 1
January 18, 2018 (Thursday)
Venue: Lounge in Front of C5
Arrival Registration 09:30~18:25
Afternoon Conference
Venue: Room C5 of Global Front (17th Floor)
13:00~13:05 Opening Remarks (Prof. Tomohiro Araki)
13:05~13:35 Keynote Speech I (Prof. Kenta Nakai)
13:35~14:05 Keynote Speech II (Prof. Sung Wing Kin, Ken)
14:05~14:35 Keynote Speech III (Prof. Manoj R. Tarambale)
Session 1: 14:35~16:20
Venue: Room C5 of Global Front
(17th Floor)
7 presentations-Topic: “Analysis of
Protein Structure and Function”
Session 2: 14:35~16:20
Venue: Room C6 of Global Front
(17th Floor)
7 presentations-Topic: “Gene
Expression and Analysis”
Venue: Lounge in Front of C5
16:20~16:40 Coffee Break & Group Photo Taking
Session 3: 16:40~18:25
Venue: Room C5 of Global Front
(17th Floor)
7 presentations-Topic: “Enzyme
Engineering and Application”
Session 4: 16:40~18:25
Venue: Room C6 of Global Front
(17th Floor)
7 presentations-Topic: “Medical Image
Processing”
Poster Session: 13:00~18:25 Venue: Lounge in Front of C5
Day 2
January 19, 2018 (Friday) 09:30 ~18:50
Morning Conference
Venue: Room C5 of Global Front (17th Floor)
09:30~09:35 Opening Remarks (Prof. Sung Wing Kin, Ken)
09:35~10:05 Keynote Speech IV (Prof. Satoru Miyano)
10:05~10:35 Keynote Speech V (Prof. Chan Jin Park)
10:35~11:05 Coffee Break & Group Photo Taking in the Lounge
11:05~11:35 Plenary Speech I (Assoc. Prof. Hiroyuki KUDO)
11:35~11:55 Invited Speech I (Assoc. Prof. Siew Woh Choo)
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Day 2
Lunch 12:00~13:00 Venue: Lounge in Front of C5
Afternoon Conference
Venue: Room C5 of
Global Front (17th
Floor)
Venue: Room C6 of
Global Front (17th
Floor)
Venue: Conference
Room 1 of University
Hall (3rd Floor )
13:00~13:30
Plenary Speech II
(Prof. Keimei Oh)
13:00~13:30
Plenary Speech III
(Assoc. Prof. Riichi
Kajiwara)
13:00~13:30
Keynote Speech VI
(Prof. Harold Yih-Chi
Tan)
Session 5: 13:30~16:00
10 presentations-Topic:
“Molecular Biology and
Bioinformatics”
Session 6: 13:30~16:00
10 presentations-Topic:
“Biomedical
Engineering”
Session 7: 13:30~16:00
10 presentations-Topic:
“Microorganism and
Ecosystem”
Venue: Lounge in Front of C5
16:00~16:20 Coffee Break
Session 8: 16:20~18:50
10 presentations-Topic:
“Gene Expression and
Analysis”
Session 9: 16:20~18:50
10 presentations-Topic:
“Basic Medicine and
Drug Development”
Session 10: 16:20~18:50
10 presentations-Topic:
“Environmental Quality
Assessment and
Management”
Dinner 19:00 Venue: Lounge in Front of C5
Day 3
January 20, 2018 (Saturday) 09:30~17:30
One Day Visit and Tour
09:30~10:00 Departure from APA Hotel Kanda Jimbocho Ekihigashi
10:00~11:00 Visit Meiji University Museum
11:00~12:00 Have Lunch in Local Restaurant
12:30~17:30 City Tour
17:30~ Go Back to APA Hotel Kanda Jimbocho Ekihigashi
Tips: Please arrive at the Conference Room 10 minutes before the session begins to upload PPT into
the laptop.
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Detailed Schedule for Conference
January 18, 2018 (Thursday)
Venue: Lounge in Front of C5
09:30~18:25 Arrival and Registration
Afternoon, January 18, 2018 (Thursday)
Venue: Room C5 of Global Front (17th Floor)
13:00~13:05
Opening Remarks
Prof. Tomohiro Araki
Tokai University, Japan
13:05~13:35
Keynote Speech I
Prof. Kenta Nakai
University of Tokyo, Japan
Topic: “Analyses of Transcriptional Regulatory Codes through a
Variety of NGS Data”
13:35~14:05
Keynote Speech II
Prof. Sung Wing Kin, Ken
National University of Singapore, Singapore
Topic: “Fast, Sensitive and Accurate Detection of Virus Integrations
in Cancer”
14:05~14:35
Keynote Speech VI
Prof. Manoj R. Tarambale
Marathwada Mitra Mandal’s College of Engineering, Pune, India
Topic: “Detection of Medical Disease (Lung Cancer) Using Image
Processing Tool–An Engineering Approach”
Morning, January 19, 2018 (Friday)
Venue: Room C5 of Global Front (17th Floor)
09:30~09:35
Opening Remarks
Prof. Sung Wing Kin, Ken
National University of Singapore, Singapore
09:35~10:05
Keynote Speech III
Prof. Satoru Miyano
The University of Tokyo, Japan
Topic: “Unraveling Cancer Systems Disorders from Big Data by
Supercomputers”
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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10:05~10:35
Keynote Speech IV
Prof. Chan Jin Park
Incheon National University, South Korea
Topic: “Development of Air Quality Management in Urban Cities”
11:05~11:35
Plenary Speaker I
Assoc. Prof. Hiroyuki KUDO
Meiji University, Japan
Topic: "Electrochemical Biosensors for Healthcare IoT"
11:35~11:55
Invited Speaker
Assoc. Prof. Siew Woh Choo
Xi'an Jiatong-Liverpool University, China
Topic: “Pangolin Genomes and the Evolution of Mammalian Scales
and Immunity”
12:00~13:00 Lunch Time
Venue: Lounge in Front of C5
Afternoon, January 19, 2018 (Friday)
Venue: Room C5 of Global Front (17th Floor)
13:00~13:30
Plenary Speaker II
Prof. Keimei Oh
Akita Prefectual University, Japan
Topic: "Chemical Genetics Strategy Identifies Small Molecules Induce
Triple Response in Arabidopsis"
Venue: Room C6 of Global Front (17th Floor)
13:00~13:30
Plenary Speaker III
Assoc. Prof. Riichi Kajiwara
Meiji University, Japan
Topic: "Network Properties of Limbic Neurons Revealed by
Voltage-Sensitive Dye Imaging"
Venue: Conference Room 1 of University Hall (3rd Floor )
13:00~13:30
Keynote Speech V
Prof. Harold Yih-Chi Tan
National Taiwan University, Taiwan
Topic: “University Capacity Building to Help Local Government Setup
Resilient Community in Taiwan”
Note: (1) The registration can also be done at any time during the conference.
(2) The organizer doesn’t provide accommodation, and we suggest you make an early reservation.
(3) One Best Oral Presentation will be selected from each oral presentation session, and the Certificate for
Presentation will be awarded at the end of each session on January 18, 2018 and January 19, 2018.
Let’s move to the session!
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Parallel Session Index
January 18, 2018
Session Time Venue Session Topic
1
(P-24~P-27)
14:35~16:20 Room C5 of Global
Front (17th Floor)
Analysis of Protein
Structure and Function
B0038, B0078, B0082, B0098, B0020, B0025, B0085
2
(P-28~P-32)
14:35~16:20
Room C6 of Global
Front (17th Floor)
Gene Expression and
Analysis
B0011, B0032, B0059, B0062, B0069, B0074, B0077
3
(P-33~P-37)
16:40~18:25 Room C5 of Global
Front (17th Floor)
Enzyme Engineering
and Application
B0073, B0010, B0013, B0033, B0052, B0084, B0092
4
(P-38~P-42)
16:40~18:25 Room C6 of Global
Front (17th Floor)
Medical Image
Processing
B0008, B0039, B0043, B0045, B0046, B0051, B0110
January 19, 2018
Session Time Venue Session Topic
5
(P-43~P-48)
13:30~16:00 Room C5 of Global
Front (17th Floor)
Molecular Biology and
Bioinformatics
B0034, B0107, T1001, B0021, B3013, B0041, B3011, B0104, B0108, T0008
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Session Time Venue Session Topic
6
(P-49~P-54)
13:30~16:00 Room C6 of Global
Front (17th Floor)
Biomedical
Engineering
T0005, B0018, B0035, B0044, B3012, T0015, B0040, B0003, B0031, B0093
7
(P-55~P-60)
13:30~16:00 Conference Room 1 of
University Hall (3rd
Floor )
Environmental Quality
Assessment and
Management
T0003, T0006, T0014, T0021, T0011, T0013, T2005, T1002, T0004, T0023
8
(P-61~P-66)
16:20~18:50 Room C5 of Global
Front (17th Floor)
Gene Expression and
Analysis
B0081, B0086, B0088. B0114, B0076, B0019, B0115, B0036, B0099, B0014
9
(P-67~P-72)
16:20~18:50 Room C6 of Global
Front (17th Floor)
Basic Medicine and
Drug Development
B0058, B0067, B0075, B0083, B0087, B0096, B0027, B0055, B0071, B0079
10
(P-73~P-78)
16:20~18:50 Conference Room 1 of
University Hall (3rd
Floor )
Microorganism and
Ecosystem
T0010, T3001, T3002, T3003, T3004, T3007, T0016, T4006, T0028, T0009
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Session 1
Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session.
Afternoon, January 18, 2018 (Thursday)
Time: 14:35~16:20
Venue: Room C5 of Global Front (17th Floor)
Topic: “Analysis of Protein Structure and Function”
Session Chair: Prof. Kenta Nakai
B0038
Presentation 1
(14:35~14:50)
Coarse-Grained Molecular Dynamics Simulation of Sulerythrin and LARFH
for Producing Protein Nanofibers
Takashi Ozawa, Hironao Yamada, Takeshi Miyakawa, Ryota Morikawa,
Sota Yagi, Satoshi Akanuma, Akihiko Yamagishi and Masako Takasu
Tokyo University of Pharmacy and Life Sciences, Japan
Abstract—Artificial creation of fibers utilizing proteins has been a target of
bionanotechnology. Yagi et al. succeeded in designing artificial protein
fibers using two types of proteins: LARFH and sulerythrin. Binding
interfaces were designed for sulerythrin and LARFH by introducing
mutations, and the fibrous structures were confirmed by atomic force
microscopy. However, branching was observed in the fibrous structure,
possibly because of non-specific interactions between the proteins. In this
study, we analyzed the behavior and binding sites of sulerythrin mutants and
LARFH mutants using coarse-grained molecular dynamics (MD) simulation.
Binding simulations were performed for a system of one sulerythrin and one
LARFH, and also of two sulerythrin molecules and four LARFH molecules.
These results suggested that glutamic acids originally possessed by
sulerythrin contribute to non-specific binding at sites other than the designed
interfaces.
B0078
Presentation 2
(14:50~15:05)
Isolation and Characterization of Collagen from Red Cheek Barb Scale
(Puntius orphoides)
Sawanit Aichayawanich and Thanya Parametthanuwat
King Mongkut’s University of Technology North Bangkok, Thailand
Abstract—This research involved experimental investigation isolation of
some characteristics of collagen from red cheek barb scale which is the
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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waste from pickled fish factory. Firstly, the effect of decalcification
conditions including HCl solution concentration (0.8, 1.0, 1.2 N) and
decalcification time (4, 6 h) on ash content of the scale was studied. The
result showed that the lowest ash content was found when decalcification at
highest HCl solution concentration and decalcification time. The decalcified
scales which had the lowest ash content were then extracted the collagen
using three interesting methods to receive acid soluble, pepsin soluble, and
acid soluble - heat collagens. Yields of acid soluble, pepsin soluble, and acid
soluble - heat collagens were 0.43, 0.60 and 0.77% (based on dry weight),
respectively. Tmax of acid soluble, pepsin soluble, and acid soluble - heat
collagens were in the range of 29.2-31.7oC. Tmax of acid soluble - heat
collagen was lowest. L*, a*, and b* values of the collagens were slightly
affected by the extracting method. However, sensory evaluation scores of the
collagens were not depended on extracting methods.
B0082
Presentation 3
(15:05~15:20)
Improvement of Template-Based Protein Structure Prediction by using
Chimera Alignment
Shuichiro Makigaki and Takashi Ishida
Tokyo Institute of Technology, Japan
Abstract—The determination of a protein’s structure provides important
information that can be used for various practical applications in the
biological sciences, such as virtual screening, function prediction, etc.
Protein structures can be precisely predicted using template-based modeling
if we can find good template structures from a database. However, such
predictions sometimes fail even if a template with sufficient quality is found
because the sequence alignment used for the modeling is incorrect.
In this paper, we propose a new method for improving sequence alignment
in single-template-based modeling. The sequence alignments used as an
input of template-based modeling are normally generated by homology
search tools, and the alignments vary depending on the search algorithm
used. Each single alignment is often imperfect, but most of them have
suitable parts for template based modeling at different positions. Thus, a
profile of multiple alignments is typically constructed to obtain a consensus
among the alignments by multiple template search tools. Integrated
alignments are generated by random sampling, and the final prediction
model is selected based on model quality assessment scores and the joint
probability of the profile.
We performed evaluation tests using template-based modeling targets in
CASP11 and compared the proposed method to several existing major
alignment algorithms. The results showed that the proposed method could
improve the model accuracy of single-template modeling.
B0098 Development of Ocular Surface Tribometer and Frictional Characteristics of
Human Ocular Surface
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Presentation 4
(15:20~15:35)
Sarwo Pranoto, Shingo Okamoto, Ryoichiro Kataoka, Jae Hoon Lee,
Atsushi Shiraishi, Yuri Sakane, Masahiko Yamaguchi and Yuichi Ohashi
Ehime University, Japan
Abstract—The purpose of this research is to determine the frictional
characteristics of human ocular surfaces. An ocular surface tribometer was
developed to measure the normal forces, frictional forces, and velocities of
the probe on human ocular surfaces simultaneously. In this research, the
measurement on six trial subjects was performed with the developed ocular
surface tribometer. In addition, a mathematical model was proposed to
describe the frictional coefficient of the human ocular surface. The frictional
characteristic curves were found by using the computational program
employing BSG-Starcraft of PSO and LSM developed by the authors in the
previous research. It was possible to classify the frictional characteristics of
human ocular surfaces into three types.
B0020
Presentation 5
(15:35~15: 50)
Targeting lncRNA ASBEL with antagoNAT for Breast Cancer Therapy
Yang Xia and Wang Sheng
Beijing University of Technology, China
Abstract—LncRNAs are RNA transcripts longer than 200nt that lack
protein-coding potential. LncRNA ASBEL has been identified as an
anti-sense transcript of BTG3 gene, which encodes an anti-proliferation
protein. Remarkable down-regulation of BTG3 has been reported in
triple-negative breast cancer (TNBC). In the present study, a number of
single-stranded modified antisense DNA oligonucleotides (antagoNAT) were
designed, synthesized and screened for specific lncRNA ASBEL
knockdown. The antagoNAT played a significant tumor suppressive role in
TNBC by effective down-regulating lncRNA ASBEL, which in turn led to
increased BTG3 expression. We also managed the co-delivery of antagoNAT
and curcumin by using hyaluronic acid-chitosan nanoparticles for TNBC
therapy. The obtained data suggest that the nanosystem-based co-delivery of
antagoNAT and curcumin could achieve synergistic effects on tumor
suppression.
B0025
Presentation 6
(15:50~16:05)
Machine Learning in Predicting Hemoglobin Variants
Monalisha Saikia Borah, Bikram Pratim Bhuyan, Mauchumi Saikia Pathak
and P. K. Bhattacharya
Asian Institute of Management & Technology, India
Abstract—Disease diagnosis is of utmost importance in providing proper
medical treatment. Genetic diseases like hemoglobinopathies and
thalassemia needs to be diagnosed accurately and on time. Though Hb
variants is diagnosed using HPLC based hemoglobin typing machine but
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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still proper interpretation of the data obtained is necessary and this requires
trained professionals. Machine learning helps in interpreting the obtained
data and helps in predicting the type of Hb variants, thus reducing the work
of health professionals. In this study, the prepared data are classified using
the following classifiers namely Logistic Regression, Support Vector
Classifier (SVC), k-Nearest Neighbor (KNN), Gaussian Naïve Bayes,
Perceptron Classifier, Linear SVC, Stochastic Gradient Descent, Decision
Tree, Random Forest and Multi-layer Perceptron. The pre-processing,
visualization and the classification step were implemented using Python 2.7
with an Intel Core i5 computer. The performance of each classifier is then
tested by first creating a confusion matrix. Indices like ‘precision’, ‘recall’
and ‘f1-score’ are used to quantify the quality of each model. KNN,
Decision Tree and Random Forest shows better classification results in
comparison to the other classifiers. With a precision of 93.89%, recall of
92.78% and f1-score of 93.33%, the decision tree and random forest
classifiers proves to be better classifiers in predicting Hb variants with a
higher accuracy rate.
B0085
Presentation 7
(16:05~16:20)
Study of Cytosolic and Mitochondrial H2O2 Production in LPS-Induced
Microglia Cells Using the H2O2-Sensitive Protein HyPer
Minkyoung Kam, Hanseop Kim and Dong-Seok Lee
Kyungpook National University, South Korea
Abstract—Dysregulation of the production of pro-inflammatory mediators
in microglia exacerbates the pathologic process of neurodegenerative
disease. ROS actively affect microglia activation by regulating transcription
factors that control the expression of pro-inflammatory genes. However,
accurate information regarding the function of ROS in different subcellular
organelles has not yet been established. Here, we analyzed the pattern of
cytosolic and mitochondrial H2O2 formation in LPS-activated BV-2
microglia using the H2O2-sensitive protein HyPer targeted to specific
subcellular compartments. Our results show that from an early time,
cytosolic H2O2 started increasing constantly, whereas mitochondrial H2O2
rapidly increased later. In addition, we found that MAPK affected cytosolic
H2O2, but not mitochondrial H2O2. Consequently, our study provides the
basic information about subcellular H2O2 generation in activated microglia,
and a useful tool for investigating molecular targets that can modulate
neuroinflammatory responses.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Session 2
Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session.
Afternoon, January 18, 2018 (Thursday)
Time: 14:35~16:20
Venue: Room C6 of Global Front (17th Floor)
Topic: “Gene Expression and Analysis”
Session Chair: Prof. Sung Wing Kin, Ken
B0011
Presentation 1
(14:35~14:50)
Comparative Genomic Analysis of Cellulosimicrobium Cellulans and
Phylogenetic Species Searching for Cellulosome-Like Multiprotein
Complex-Encoding Genes
Tongyi Dou, Ran Duan, Chenglin Liu and Le Liu
Dalian University of Technology, China
Abstract—Objective: Previously, we have reported the discovery of a
cellulosome-like multiprotein complex produced by the wild type strain
F16 identified as Cellulosimicrobium cellulans and the structure-function
studies revealed by genomic and proteomic techniques. This work aimed at
comparative genomic analyzing of Cellulosimicrobium cellulans and
phylogenetic-related species, the species of family
Promicromonosporaceae and family Cellulomonadeceae, searching for
cellulosome-like multiprotein complex-encoding genes. Methods: Firstly,
comparative genomic alignment study of the 13 phylogenetic species was
performed using CVTree. Secondly, on protein level, 27 protein sequences
previously identified as subunits of the cellulosome-like complex were
searched by Blastp using the 13 protein-sets as local database. Results:
CVTree results revealed that strain F16 is most close to
Cellulosimicrobium funkei JCM 14302, which is in confliction with the
16S rRNA phylogenetic results whereas strain F16 is most close to
Cellulosimicrobium cellulans JCM9965. Since whole genomic
comparative analysis is more reliable to the 16S rRNA results, this result
may overturn previous conclusions. Moreover, according to local Blastp
results, gene M768_06655, M768_10315, and M768_18415 were
identified in consistent with the production of the cellulosome-like
complex. Conclusion: This work provides clues and predictions for
uncovering molecular mechanism of cellulosome-like complex production
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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by species of family Promicromonosporaceae and family
Cellulomonadeceae.
B0032
Presentation 2
(14:50~15:05)
Simulation Study for Wild Type and C101F Mutant of LIM2 Domain in
FHL1
Hiroki Takeuchi, Hiroki Okajima, Hironao Yamada, Takeshi Miyakawa,
Ryota Morikawa, Masako Takasu and Yukiko Hayashi
Tokyo University of Pharmacy and Life Sciences, Japan
Abstract—Myopathy is a rare disease lacking a fundamental therapy.
Several genetic factors are involved in myopathy; those caused by
mutations in FHL1 are rare. We performed molecular dynamics simulation
of the LIM2 domain in FHL1 (four and a half LIM domain protein 1). We
simulated a partial system consisting of only the LIM2 domain for the
wild-type and C101F mutant to confirm the structural stability. We found
that structural changes and fluctuations were larger for the mutant type
than for the wild-type. Therefore, mutant type structures are unstable in
water when the mutations are in residues constituting the zinc finger.
Similar results were observed in the simulation of the LIM1+LIM2
domain.
B0059
Presentation 3
(15:05~15:20)
Alignment of Unaligned Reads for the Detection for Novel Genes Using
the Hanwoo Whole Genome
Kelsey Caetano-Anolles, Kwondo Kim, Woori Kwak, Samsun Sung,
Dajeong Lim, Bong-Hwan Choi and Heebal Kim
Seoul National University, laboratory of bioinformatics and population
genetics, South Korea
Abstract—Hanwoo, also known as Korean native cattle, are a breed of
cattle world renowned for the high percentage of fat marbling in their meat.
This breed has begun to be used as a main source of meat in the past
century; since then, demand for this product in Korea has skyrocketed.
Genomic data gathered from 136 Hanwoo cattle was analyzed using
advanced statistical methods in this study. The primary objective of this
study was to assemble and map unaligned reads in order to identify and
predict novel genes. These genes were cross-checked using a protein
domains identifier. Cross-referencing with the Pfam database identified
associated protein domains covering a total of 178 regions. These domains
were characterized mostly by immune-related domains related to
Immunoglobulin function. Interesting was the expression of the Interferon
group of signaling proteins, which is crucial for immune system response.
Although research into the genetic aspects of and expression of genes
related to immunoglobulin has been widely conducted in humans and mice,
research in this field is lacking when it comes to livestock breeds,
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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particularly cattle. This kind of information can be used to create synthetic
recombinant species-specific antibodies, which could be used to treat and
prevent infectious diseases.
B0062
Presentation 4
(15:20~15:35)
Regulation of Host Genome Methylation and Expression in HIV Infection
Xi Zeng and Kwok Wing Stephen Tsui
The Chinese University of Hong Kong, Hong Kong
Abstract—It has been shown that boththe methylationand gene
expressionplay rolesin HIV-host interplay.In this study, tofurther
investigatethe relationship between HIV infection andpatterns of
genome-wide methylation and gene expression, we performedMeDIP-seq
and RNA-seqfor five T cell linesand each cell line contained HIV+ and
control samples.Withindifferentially expressed genes (DEGs)between
HIV+ and control samples,the top enriched pathways included “primary
immunodeficiency” and “inflammatory response”. Eighteenup-regulated
and eightdown-regulated DEGswere shared byfourcell lines. Moreover, on
average,1,736differentially methylated regions (DMRs)were found
betweenHIV+ and control samplesfor each cell line. DMRs were
significantly enriched in promoter and exon regions. The top enriched
pathways ofDMR-associated genes were“ubiquitin-dependent protein
catabolic process”and “GTPase activator activity”.Besides, 635 genes were
also involvedin the crosstalkof methylation and expression, out of which
ATP1B1, CAMK2D, GRIN2A, MAPK10, CACNA1C and F2R were shared
by more than two cell lines and enrichedin “cAMP signaling pathway”,
which affectsHIVreplication and infection.This study broadens our
understanding on the mechanism of HIV/AIDSandprovidesa resource
about dynamic changesat thetranscriptome and epigeneticlevelsduringHIV
infection.
B0069
Presentation 5
(15:35~15: 50)
Iterative Scaling Fuzzy Additive Spectral Clustering (is-FADDIS)
Algorithm for Subtypes Cancer Identification using Gene Expression Data
Ito Wasito, Muhammad Faturrachman, Ionia Veritawati, Indra Budi and
Betha Nurinasari
Universitas Indonesia, Indonesia
Abstract—Cancer is one of main non-communicable disease. Analysis of
subtypes cancer identification is important to determine the proper
treatment for each patient. However, cancer data analysis is difficult
problem since it has multiple risk factors genes and clinical condition of
patients. This study aims to identify subtypes cancer using Iterative
Scaling-FADDIS (is-FADDIS) algorithm which extends the FADDIS
algorithm in sparse data problems. In this research, is-FADDIS technique is
applied to microarray data to identify subtypes cancer in two data sets:
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Colorectal Carcinoma and B-Cell Lymphoma. Then, the clusters structure
those to be found will be validated based on Correlation Ratios and Global
Silhoutte. For Colorectal Carcinoma cancer, the correlation ratio between
cluster and the existence of tumour will be analyzed. The results of
experiments show that is-FADDIS works well on both data sets. For
Colorectal Carcinoma cancer data, the results show that those clusters to be
found by is-FADDIS has successfully correlated with the existence of
tumour. For B-Lymphoma dataset, is-FADDIS algorithm has successfully
well separated two clusters of cancer subtypes with Global Silhoutte value
0.4035.
B0074
Presentation 6
(15:50~16:05)
Exploring Genetic Variation in 40 Pigmented Rice Varieties via Whole
Genome Sequencing Data for Rice Breeding
Rabiatul Adawiah Zainal Abidin, Zeti Azura Mohamed Hussein and
Norliza Bakar
National University of Malaysia (UKM), Malaysia
Abstract—Single nucleotide polymorphisms (SNPs) provide a valuable
resource in understanding the genetic basis of pigmented rice varieties.
Unfortunately, not much analysis has been done on the SNPs of pigmented
rice varieties. We have performed a comprehensive bioinformatic analysis
on the 40 varieties of pigmented rice whole genome data to identify SNPs
that are involved in the production of flavonoids and other compounds with
antioxidant properties. Out of 2,460,172 high-quality SNPs discovered,
8514 of these SNPs are polymorphic. Gene ontology (GO) enrichment
analysis revealed the classification of these polymorphic SNPs in various
biological processes such as cellular metabolic process, secondary
metabolite biosynthesis, amongst others. In addition, some genic SNPs
were found in genes encoding enzymes of flavonoid biosynthesis in both
black (4119 SNPs) and red (6369 SNPs) rice genomes. From these
combined datasets, 2138 non-synonymous SNPs were identified where this
shows possible mutations in the genes encoding enzymes of flavonoid
biosynthesis; i.e. encoding for anthocyanin in black rice and
proanthocyanidin in red rice. Results of this study can be used as a
resource to be applied in rice breeding program towards the improvement
of its nutritional value.
B0077
Presentation 7
(16:05~16:20)
Selection of the Most Informative Schemes for Multi-Locus Sequence
Typing
Ji-Wei Lin, Yen-Yi Liu and Chih-Chieh Chen
National Sun Yat-sen University, Taiwan
Abstract—To investigate and surveillance outbreak of disease we need
molecular fine subtyping. We present a website tool, PGAdb-builder.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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PGAdb-builder can establish a pan-genome allele database (PGAdb) and
whole genome multilocus sequence typing (wgMLST) approach applied to
achieve subtyping layout that can be comparable among laboratories. We
build a PGA database of Salmonella enterica Serovar Typhimurium as a
test for the website. The dendrogram (genetic relatedness tree) shows
epidemiologically well-characterized S.Typhimurium isolates. But large
amount locus of the strain makes the subtyping process time-consuming.
We implement Python program to improve the situation by making use of
some feature selection methods (variance threshold, univariate selection,
recursive feature elimination, principal component analysis). These
methods utilized to reduce the quantity of the locus by removing low
influence or useless ones. Decreased strain locus dataset can create similar
group of outbreak strain compared with the raw dataset result. Employing
the feature selection method can be a more efficient way to reach the same
goal.
Coffee Break & Group Photo Taking
16:20~16:40 Lounge in Front of C5
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
- 33 -
Session 3 Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session. Afternoon, January 18, 2018 (Thursday)
Time: 16:40~18:25
Venue: Room C5 of Global Front (17th Floor)
Topic: “Enzyme Engineering and Application”
Session Chair: To be added
B0073
Presentation 1
(16:40~16:55)
Metabolic Engineering of Extended MEP Pathway for Production of
Cis-Abienol and Isoprene
Egils Stalidzans, Katrina Daila Neiburga, Vitalijs Komasilovs and Agris
Pentjuss
Latvia University of Agriculture, Latvia
Abstract—To utilize the potential of plants as “green factories”, new
regulatory and biosynthesis pathways have to be introduced and correctly
expressed to maximize productivity. Metabolic modeling is applied to test
the opportunities of production of cis-abienol and isoprene in Arabidopsis
thaliana as model plant. Kinetic models of 2-C-methylerythritol 4-phosphate
(MEP) pathway with extensions towards production of 1) isoprene and 2)
cis-abienol are developed and optimised for better yield. Total optimization
potential (TOP) approach is applied to find minimal enzyme set necessary to
reach requested fraction of TOP without full combinatorial search of
adjustable parameter (enzyme concentrations) combinations. Total enzyme
activity constraint is applied to represent the limited capacity of protein
building machinery. Homeostatic constraint is applied to limit the impact of
intermediate metabolite concentration changes on reactions outside the
scope of the model. The initial values of carotenoids, chlorophyll and
plastoquinones fluxes are fixed during optimization. COPASI software and
COPASI wrapper SpaceScanner are used. Several metabolic designs (sets of
enzyme concentration changes) are proposed for experimental
implementation.
B0010
Presentation 2
Structure-Activity Relationship Study of Pentacyclic Triterpenoids as Potent
Inhibitors against Human Carboxylesterase 1
Yangliu Xia, Tongyi Dou, Lili Jiang, Yong Liu and Guangbo Ge
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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(16:55~17:10)
Dalian University of Technology, China
Abstract—Human carboxylesterase 1 (hCE1), one of the most important
serine hydrolases distributed in liver and adipocytes, plays key roles in
endobiotic homeostasis and xenobiotic metabolism. In the present study, a
series of pentacyclic triterpenoids were selected as the model ligands and in
vitro inhibitory activities against hCE1 were tested. By using the
three-dimensional quantitative structure-activity relationships (3D-QSAR)
method of comparative molecular field analysis (CoMFA), the structure and
activity relationship of these compounds was studied. The 3D-QSAR modes
displayed good correlation between the variations on percent inhibition and
the steric-electrostatic properties, with the cross-validation coefficient,
non-cross validation coefficient and external verification of the complex
correlation coefficient of 0.512, 1, and 0.72, respectively. Converting the
C-3 keto of the pentacyclic ring to hydroxyl, and C-17 hydroxymethyl to
carboxyl group, led to a significant enhancement of the inhibitory effect on
hCE1. These exciting findings inspired us to design and synthesize the more
potent compound (IC50 0.015 μM) as a novel inhibitor against hCE1, which
was 897-fold more potent than the parent compound betulin. All these
findings will be implicated for the design and further optimization of potent
hCE1 inhibitors, which could serve as a promising tool for exploring drug
candidates for clinical applications in selectively modulating
hCE1-associated toxicities.
B0013
Presentation 3
(17:10~17:25)
NEDD4 is Down-Regulated by OTU-T Deubiquitinating Enzyme
Jun-Hyeok Park, Soo-Yeon Kim, Da-Hye Lee and Kwang-Hyun Baek
CHA University, Republic of Korea
Abstract—Deubiquitination is an inverse process of ubiquitination mediated
by deubiquitinating enzymes (DUBs). DUBs have the ability to deconjugate
ubiquitin molecules from ubiquitinated substrates and stabilize them. Thus,
DUBs have been considered to be pivotal mediators in diverse cellular
systems including various signal transduction pathways such as cell death
and cell proliferation. OTU-T, one of DUBs, which is a member of the
ovarian-tumor protease (OTU) family, and is found that it interacts with
Neural precursor cell Expressed, Developmentally Down-regulated 4
(NEDD4) through bioinformatics tools. OTU-T is one of the ubiquitin E3
ligases, which is involved in the regulation of diverse processes including
insulin-like growth factor signaling and neural architecture. In this study, we
demonstrated that OTU-T binds to NEDD4, and NEDD4 interacts with
OTU-T regulating cell proliferation. And knock-out and overexpression
studies were performed to investigate the effect of OTU-T on cell
proliferation. Immunoblotting and colony forming assays revealed that the
level of NEDD4 protein was decreased with a gradual increase of OTU-T
expression, while the rate of cell proliferation was decreased in a
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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dose-dependent manner of OTU-T expression. Important directions for
future studies will include the analysis of mechanisms that regulate cell
proliferation mediated by Hippo signaling pathway.
B0033
Presentation 4
(17:25~17:40)
Differentially expressed deubiquitinating enzymes in the presence of toxic
environmental chemicals
Youngjae Hwang, Da-Hye Lee, Jihye Choi and Kwang-Hyun Baek
CHA University, Republic of Korea
Abstract—Premature ovarian failure (POF) is the loss of function for the
ovaries in women less than 40 years of age. Diseased POF ovaries cannot
produce a normal amount of estrogen and cannot ovulate periodically,
resulting in infertility. However, the cause of POF is not fully understood
yet. To investigate the differential expression of deubiquitinating enzyme
(DUB) genes in patients with POF, we developed a POF model by treating
with toxic environmental factors including formaldehyde, which might
cause POF in human. Using this model, a multiplex RT-PCR assay was
performed to identify putative biomarkers by comparing expression levels of
DUB genes encoding cysteine proteases. Interestingly, the assay revealed
that the level of expression for POF-DUB1, POF-DUB2 and POF-DUB3
was increased. The difference in the expression level of several proteolytic
enzyme genes was confirmed by repeated experiments, and the possibility of
using them as biomarkers to diagnose POF was demonstrated.
B0052
Presentation 5
(17:40~17:55)
Uses of Copra Waste and Wheat Bran for Cellulase Production by
Trichoderma reesei in Solid State Fermentation
Teerin Chysirichote, Lexpet Nareerat, Kaisaard Sawwanit and Chysirichote
Teerin
King Mongkut’s Institute of Technology Ladkrabang, Thailand
Abstract—The potential use of copra waste from coconut pressing process
for cellulase production by solid-state fermentation (SSF) was studied in this
research. Trichoderma reesei TISTR3080 was cultivated at 30°C for 7 days
on the copra waste (CW) with and without supplementary of wheat bran
(WB) as a nitrogen source. The results showed that adding WB increased
both the fungal growth and productions of cellulase including activities of
total cellulase, CMCase and β-glucosidase. The fungal growth which was
indirectly measured by glucosamine measurement increased from 0.33 d-1
in the CW without WB to 1.1 d-1 in the CW added WB at the ratio of 3:3.
Moreover, adding WB to CW at the ratio of 3:3 accelerated the activity of
β-glucosidase from 12.6 to 16.8 U/ g dry substrate at 1 d. However,
supplementing CW with WB at the ratio of 3:2 enhanced the total cellulase
activity and CMCase activity from 0.31 to 5.23 FPU/ g dry substrate at 2 d
and 0.42 to 5.18 U/ g dry substrate at 6 d, respectively.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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B0084
Presentation 6
(17:55~18:10)
The Epithelial-Mesenchymal Transition in Colon Cancer is Promoted by
Peroxiredoxin 5
Jin-Woo Yoo, Kyung-Min Kim and Dong-Seok Lee
Kyungpook National University, Republic of Korea
Abstract—Globally, colorectal cancer (CRC) is common cause of
cancer-related deaths. The high mortality rate of patients with colon cancer
is due to cancer cell invasion and metastasis. Initiation of the
epithelial-to-mesenchymal transition (EMT) is essential for the
tumorigenesis. Peroxiredoinxs (PRX1-6) have been reported to be
overexpressed in various tumor tissues, and involved to be responsible for
tumor progression. However, the exact role of PRX5 in colon cancer
remains to be investigated enhancing proliferation and promoting EMT
properties. In this study, we constructed stably overexpressing PRX5 and
suppressed PRX5 expression in CRC cells. Our results revealed that PRX5
overexpression significantly enhanced CRC cell proliferation, migration,
and invasion. On the other hand, PRX5 suppression markedly inhibited these
EMT properties. PRX5 was also demonstrated to regulate the expression of
two hallmark EMT proteins, E-cadherin and Vimentin, and the
EMT-inducing transcription factors, Snail and Slug. Moreover, in the
xenograft mouse model, showed that PRX5 overexpression enhances tumor
growth of CRC cells. Thus, our findings first provide evidence in CRC that
PRX5 promotes EMT properties by inducing the expression of
EMT-inducing transcription factors. Therefore, PRX5 can be used as a
predictive biomarker and serves as a putative therapeutic target for the
development of clinical treatments for human CRC.
B0092
Presentation 7
(18:10~18:25)
In silico identification of novel kinase inhibitors by targeting PIM1 from
natural products database
Tian Yu, Youling Sun, Xinjie Lian, Feng Liu and Zheng Shi
Chengdu University, China
Abstract—Kinase provides a rich and diverse source of potential targets for
blocking tumor growth and survival. And, protein kinase inhibitors have
made a substantial beneficial impact on the therapeutic care of cancer
patients. The PIM family of serine/threonine kinases is named due to their
mode of discovery as proviral common integration sites in moloney murine
leukemia virus-induced lymphomas. PIM kinases are involved in a number
of signaling pathways which plays a vital role in cancer cells. They act in
downstream effector functions as inhibitors of apoptosis and as positive
regulators of G1-S phase progression through the cell cycle. Since virtual
screening has become an integral part of the drug discovery process, it is of
great significant to identify novel PIM inhibitors by structure-based virtual
screening. In this study, we carried out a series of elegant bioinformatics
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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approaches, such as virtual screening, molecular dynamics simulations to
identify PIM inhibitors from Food and Drug Administration
(FDA)-approved small molecule drugs. Following the 5 ns MD simulations,
Dofetilide (ZINC49583080) were recognized as “new use” FDA-approved
drug for PIM harboring cancer patients. Together, our findings may provide
successful application of virtual screening studies in the lead discovery
process, and suggest that our discovered small molecules could be effective
PIM inhibitor candidates for further study.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Session 4 Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session. Afternoon, January 18, 2018 (Thursday)
Time: 16:40~18:25
Venue: Room C6 of Global Front (17th Floor)
Topic: “Medical Image Processing”
Session Chair: Prof. Manoj R. Tarambale
B0008
Presentation 1
(16:40~16:55)
Automatic Liver Segmentation in Abdomen CT Images using SLIC and
AdaBoost Algorithms
Mucahid Barstugan, Rahime Ceylan, Mesut Sivri and Hasan Erdogan
Selcuk University, Turkey
Abstract—This study is an implementation of liver segmentation on
abdomen CT images. The liver organ was segmented by using SLIC
super-pixel and AdaBoost algorithms. Firstly, the images were clustered by
SLIC super-pixel algorithm. Then, the liver was segmented by AdaBoost
classifier. The segmentation process was done automatically. The automatic
segmentation is based on the classification of overlapping patches of the
image. The results of automatic segmentation and manual segmentation
were compared and the efficiency of the method was observed. The best
Dice rate was obtained as 92.13% and the best Jaccard rate was obtained as
85.8% on 16 abdomen CT images.
B0039
Presentation 2
(16:55~17:10)
Efficient Vertebra Segmentation Using Graph Cuts
Jin-Yuan Hsue, Chan-Pang Kuok, Ting-Li Shen, Bing-Feng Huang and
Yung-Nien Sun
National Cheng Kung University, Taiwan
Abstract—Spine surgeries are highly dangerous when applied for treating
spine diseases or injuries. In order to achieve less tissue damage and faster
recovery, minimally invasive surgery is desirable. Before operation, the
surgeon has to precisely plan the procedures based on the
three-dimensional(3D) virtual spine model which is usually segmented from
the patient’s computed tomography(CT) images. The segmentation accuracy
of conventional methods relies on the initial contour and much efforts are
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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spent for initialization. We propose a graph cuts based segmentation method
to segment the vertebra from CT images and build a 3D model for the
surgical planning. In this study, a multi-region graph cuts algorithm with
simple initialization is applied to segment the vertebra in the axial view CT
images. The segmentation results are used to build the 3D spine model. The
average dice coefficient is 0.96. We compare the segmentation performance
with the Chan-Vese level set method. We find the proposed result is better
fitted to the ground truth and needs less computational time. Consequently,
we proposed an efficient and automatic segmentation method to segment
vertebra and build a 3D spine model for pre-operational planning.
B0043
Presentation 3
(17:10~17:25)
Quantitative Assessment of Skeletal Muscle Contusion Recovery using
Ultrasound Statistical Parameter and Image Texture Feature Extraction
Zih-You Yu, Hsiou-Yi Lin and Shyh-Hau Wang
National Cheng Kung University, Taiwan
Abstract—Contusion is a common injury and that may be further diagnosed
clinically via the evaluation of medical images by experienced experts. In
the present study, efforts were made to extensively assess the recovery of
contusion tissue using ultrasound quantitative parameters and texture
feature. The experiments were performed from SD rats where the contusion
were achieved by an impact of 600g mass and 30cm height falling from a
lab-made impact platform. The integrated backscatter (IB), statistical
Nakagami parameter, and texture features extraction using gray-level
co-occurrence matrix (GLCM) were calculated from the acquired ultrasound
radio-frequency signals and B-mode images with a 30 MHz transducer. IB
and Nakagami parameter of muscle tissue before the contusion were
estimated to be above 20 dB and 0.4, respectively. After muscle contusion,
hematoma formation in the middle of necrotic muscle fibers led both IB and
Nakagami parameter to be lower than 20dB and 0.4 respectively. Twenty
days after muscle contusion, the quantitative parameters tended to recover to
those of healthy muscles. The texture features of bruise muscle using GLCM
was also found to be less echogenicity and evenly distributed, which were
postulated owing to and the hematoma. This study validated that
high-frequency ultrasound and related parameters as well as GLCM texture
features may be a feasible diagnostic means for quantitatively characterize
the degree and recovery of muscle contusion.
B0045
Presentation 4
(17:25~17:40)
Detection of Kinetic Trajectory of Tissues in the Wrist Using Ultrasound
Images for Assessing the Degree of Carpal Tunnel Syndrome
Cheng-Che Tsai, Chia-Huang Lin and Shyh-Hau Wang
National Cheng Kung University, Taiwan
Abstract—The ultrasonic measurement of the kinetic trajectory of median
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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nerve (MN) has been found to be potentially capable of diagnosing carpal
tunnel syndrome (CTS). Previous studies were performed from healthy
subjects by using normalized cross correlation (NCC) tracking technique,
and which demonstrated to be a feasible technique but required tremendous
computational time. In this study, efforts were made to apply optical flow
tracking (OFT) and adaptive Bayesian speckle tracking (ABST) techniques
to detect the movement of MN for assessing the degree of CTS. The
measurements were performed using a commercial ultrasound scanner in
healthy subjects and CTS patients. Results showed that three techniques are
capable of detecting the movement of MN and OFT takes the least
computational time. The kinetic trajectory of healthy subjects is different
from that of CTS patients. The maximum displacement of MN for subjects is
larger than those of patients. Furthermore, the movements of tendons
surrounding MN were found to provide another information for assessing
the degree of CTS. The distance between the tendon and the membrane of
subjects tended to be larger than those of patients. Current studies
demonstrate that ultrasonic detection of movements of tissues may be a
feasible means for CTS diagnostics noninvasively.
B0046
Presentation 5
(17:40~17:55)
Classification of Ultrasonic Nakagami Parameter by AI-based Method for
Identifying Liver Fibrosis
Ting-An Wu, Bo-Yen Huang and Shyh-Hau Wang
National Cheng Kung University, Taiwan
Abstract—Clinically, the diagnostics of liver fibrosis using biopsy and
histopathological slice may achieve the highest accuracy; however, the
invasive nature of these means is still with the potential of liver
inflammation and psychological discomfort for patients. The non-invasive
ultrasound (US) images were acquired from the liver of SD rats, for which
the fibrosis was induced by 𝐶𝐶𝑙4. An AI-based Convolutional Neural
Network (CNN) model was proposed to analyze US image for classifying
the degree of normal, fibrosis and cirrhosis of the liver. The feature images
in the CNN model were extracted in the convolution layers, and then
reduced into low-order feature images in the pooling layers. Finally, the
fully-connected (FC) layers compute the weight of the feature images.
Nakagami parameter (𝑚), an US statistical parameter for quantitatively
analyzing scatterer properties, was also added into the CNN model to
increase the accuracy of assessment. Results demonstrated that 𝑚 parameter
of control group was 0.58±0.03, mild group was 0.78±0.02, and severe
group was 0.83±0.02. Previous researches demonstrated that both transfer
learning and data augmentation could increase the categories and the amount
of data. Following the inclusion of these methods, the classification accuracy
of current model could approach to 90% or better.
B0051 RFID Based Tracking and Positioning for Hospital Pneumatic Delivery
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Presentation 6
(17:55~18:10)
System
Kuo-Sheng Cheng and Yi-Syong Chen
National Cheng Kung University, Taiwan
Abstract—Although pneumatic tube delivery system has been widely used
since the late 19th century, some important issues associated with its
functions are not thoroughly investigated. One of the most common
problems is how to quickly track the carrier getting stuck in complicated
tubing system, especially in hospital. The exhaustive search for locating its
position is usually very time consuming. This paper proposes the use of
radio frequency identification (RFID) technology to track and locate the
carrier’s position during delivery. The average speed for carrier during
delivery is about 6-10 m/sec. In this high-speed applications, UHF based
RFID is considered as a suitable solution. The tags are placed on the outside
of the transport tubing system and the RFID transponder is attached fixedly
in the carrier. Each tag is coded for position or sender/receiver. The proposed
system inside the carrier contains mobile power supply, RFID reader, PCB
antenna, and Raspberry pi 3 controller with Wi-Fi module. The EPC code is
employed for designating the tube position, sending/receiving unit, and
goods inside the carrier. The programming language for software
development is Python. When the carrier is sent from the sender, the
controller will check the code as a start. Then, the series of positions’ tag are
read and stored in the memory. Afterwards, the code for receiver is check.
Once it is confirmed, the route for carrier is sent to the PC system through
Wi-Fi. If it is stuck in the tube, then the location may be alarmed for
repairmen. The proposed system is demonstrated to be feasible and helpful
in hospital application.
B0110
Presentation 7
(18:10~18:25)
Discriminative Local Feature Fusion for Ear Recognition Problem
Ibrahim Omara, Xiaoming Li, Gang Xiao, Khan Adil, and Wangmeng Zuo
Harbin Institute of Technology, China
Abstract—Ear recognition problem is known as selecting whether two ear
images belong to the same person or not, this consider as a challenge due to
variation in lighting, background, pose, scale, and occlusion. This paper
presents an improvement method for unconstrained ear recognition problem
based on local feature fusion, and further analyzes the performance and
efficiency of discriminative local feature fusion for aligned and non-aligned
ear images. Firstly, local discriminative features such as LPQ, HOG, LBP,
POEM, BSIF and Gabor features are extracted from the ear images. Then,
Discriminant Correlation Analysis (DCA) is exploited for fusion and
reduction dimension. Finally, support vector machine (SVM) is adopted for
classification. Experiments are conducted on popular ear databases, USTB I,
USTB II, and IIT Delhi II. Furthermore, we report an encouraging result on
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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a difficult and challenging ear database called annotated web ear (AWE) that
is collected from the wild. The experimental results show superior of
proposed approach that can achieve a high performance for non-aligned
images (AWE and USTB II datasets), on the other hand, unique local
features can achieve promising recognition rates for aligned images, USTB I
and IIT Delhi II datasets.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Session 5 Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session. Afternoon, January 19, 2018 (Friday)
Time: 13:30-16:00
Venue: Room C5 of Global Front (17th Floor)
Topic: “Molecular Biology and Bioinformatics”
Session Chair: Prof. Satoru Miyano
B0034
Presentation 1
(13:30~13:45)
Structural Analysis of Metal Binding Peptides using Molecular Dynamics
Mao Watabe, Hironao Yamada, Takeshi Miyakawa, Ryota Morikawa,
Masako Takasu, Tatsuya Uchida and Akihiko Yamagishi
Tokyo University of Pharmacy and Life Sciences, Japan
Abstract—Proteins that specifically bind metals have been the target of the
research for developing new organic-inorganic hybrid materials. Some
amino acid sequences that bind metal have been reported, and the
structures of proteins and peptides are considered responsible for binding
to metal. The purpose of this study is to identify molecular structures
responsible for binding metals. We performed molecular dynamics
simulations and structural analyses of metal-binding peptides. The most
frequently appearing structure of each peptide was identified. Combined
with the previous experimental results, peptides with a stable, specific bent
structure were suggested to have strong binding abilities. Peptides with a
different bent structure have been suggested to be responsible for weak
binding ability.
B0107
Presentation 2
(13:45~14:00)
mRNA Structure in Translation Regulation and its Selective Shaping on
Sequence
Yuming Xu and Wanjun Gu
Southeast University, China
Abstract—Many studies have suggested various functional roles
potentially played by the secondary structure of mRNA in gene regulation.
One of previous works demonstrated reduced stability of the mRNA
structure near the start codon in almost all cellular life and this reduction is
probably selected for efficient translation initiation. We utilized similar
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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methods to systematically further our investigation in structures around
two other regions. In animals, but not in plants, we discovered a universal
trend of increased mRNA stability near the 5’cap in mRNAs regulated by
miRNA. GC content of 5’UTR, 5’UTR length and number of miRNA
target sites may influence local structure near the 5’cap, which is likely
selected to facilitate miRNA-mediated translation regulation. Another
consistent pattern--decreased structure just before the stop codon followed
by a strong signal of increased stability--was found near the translation
termination site in many species, except in yeast and worms. This pattern is
selected for efficient bounding of termination release factors and prevents
erroneous stop codon readthrough. However, among simple organisms
readthrough heterogeneity may enhance phenotypic diversity and facilitate
their adaptation to changing environments.
T1001
Presentation 3
(14:00~14:15)
Effect of Water Hyacinth on the Mechanical, Morphological and Physical
Properties of Baked Starch Foams
Sukaree Chaireh and Kaewta Kaewtatip
Prince of Songkla University, Thailand
Abstract—The objective of this work was to study the effects of water
hyacinth (WH) on the properties of baked starch foam and compare them
with the effects of commercial cellulose (CC). The effect of the both fillers
on the properties of baked starch foams including density, morphology and
impact strength was investigated. FTIR analysis revealed the structure of
WH to be composed of cellulose, hemicelluloses and lignin, whereas CC
contains only cellulose. It was found that the impact strength of starch/WH
composite foam was greater than the impact strength of both starch/CC
composite foam and starch foam without filler by about 58% and 43%,
respectively. This increase may be the result of a more efficient transfer of
stress from the starch matrix to the filler due to the strong interaction
between the hydroxyl groups in the structure of the three-dimensional
network of lignin in WH and starch. Moreover, the starch/WH composite
foam showed the best morphology of small and uniform cell size, probably
because the WH can increase the steam bubbles in the starch batter. It can
be concluded that water hyacinth is a novel filler that plays an active role
in improving the properties of baked starch foam.
B0021
Presentation 4
(14:15~14:30)
Enumerating Chemical Mono-Block 3-Augmented Trees with Two
Junctions
Yuui Tamura, Aleksandar Shurbevski, Hiroshi Nagamochi and Tatsuya
Akutsu
Kyoto University, Department of Applied Mathematics and Physics, Japan
Abstract—Enumerating chemical graphs with given constraints on their
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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topological structure is a fundamental problem in chemoinformatics and
bioinformatics, with a wide range of applications, including structure
determination and designing novel chemical compounds. We specify the
constraint on the structure by the frequencies of all paths up to a fixed
length in the graph. Given upper and lower bounds, we consider the
problem of enumerating chemical graphs whose path frequency satisfies
one withing the given bounds. A kaugmented tree is a connected
multigraph where the number of pairs of adjacent vertices minus that of
vertices is k −1, and a monoblock k-augmented tree is a k-augmented tree
that contains one biconnected component. The biconnected component of a
monoblock 3-augmented tree with two junctions contains two vertices
joined by four internally disjoint paths. We design an algorithm for
enumerating all mono-block 3-augmented trees with two junctions that
satisfy given upper and lower bounds on path frequencies. Experimental
results indicate that our algorithm performs favorably when compared to a
state-of-the-art production program, and we obtain large numbers of
structures with up to 35 atoms excluding hydrogen, which has not been
possible with existing methods.
B3013
Presentation 5
(14:30~14:45)
Peroxiredoxin 3 Promotes Cell Motility via Modulation of the Extracellular
Matrix
Jayantha Gunaratne, Pei-Jou Chua and Boon-Huat Bay
Institute of Molecular & Cell Biology, A*STAR, Singapore
Abstract— Peroxiredoxin 3 (PRDX3), is a thiol-specific antioxidant
enzyme that reduce various cellular peroxides substrates using a
cysteine-containing active site. It is overexpressed in most cancers,
however, its exact roles and mechanisms in carcinogenesis have yet to be
fully elucidated. In this study, we employed biochemical, cell biological
and proteomics approaches to understand its roles in breast cancer. First,
we performed immunohistochemistry in breast cancer tissues that showed
that high expression of PRDX3 in breast tumor tissues compared to
adjacent normal tissues. In addition, we observed that high expression of
PRDX3 was also associated with lymph node metastasis. Biochemical
assays of PRDX3 knockdown and overexpressed MDA-MB-231 breast
cancer cell line showed that PRDX3 is positively correlated with cell
migration and invasion. To delineate PRDX3 roles in these processes, we
carried out quantitative proteomics analysis of PRDX3 knockdown and
wildtype MDA-MB-231 cells that allowed identification of 159
differentially expressed proteins (DEPs) in PRDX3 knockdown cells.
Pathway and protein network analysis of these DEPs revealed enrichment
of proteins associated with mainly extracellular remodeling processes.
Ongoing validation experiments pertaining to these processes together with
PRDX3 interactome analysis will shed light onto specific mechanisms of
PRDX3 in breast cancer cell migration and invasion.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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B0041
Presentation 6
(14:45~15:00)
Effect of Various Composition of Medium on Antioxidants and
Antioxidation Capacity in Sweet Potato (Ipomoea Batatas Lam.)
Suspension Culture
Guam-Jie Cheng and Chien-Kuo Wang
Asia University, Taiwan
Abstract—In this research, by changing growth hormone, cytokines,
sucrose, iron ions and organic nitrogen concentration, the changes of
phenol, flavonoid contents and the anti-oxidation ability in sweet potato
cell cultures were studied. Results show that increasing content of sucrose
and KNO3 in the medium resulted in significant improvement of total
phenolic compounds and flavonoids content as well as cell growth.
Changing the NH4+
and FeSO4 concentration in B5 medium could cause
the same effects except increase in cell growth. A suspension cell culture
has the highest total phenolic compound contents, 9.5mg, and flavonoid
contents, 132.7 ug, respectively; and it has the highest anti-oxidation
ability in this research, too.
A potato suspension cell culture with high phenolic compound contents,
high flavonoid contents and high anti-oxidation ability was obtained, it
could be used in further study in the potato breeding.
B3011
Presentation 7
(15:00~15:15)
Fetal Movement Detection During Pregnancy with the Electrical
Impedance Tomography
Konki Sravan Kumar, Anil Kumar Khambampati, You Jung Han, Sung
Yob Kim and Kyung Youn Kim
Jeju National University, South Korea
Abstract—An appropriate and inexpensive, continuous, non- invasive
methods are required to detect the fetal movements, which are important
and fundamental clinically significant parameters of fetal growth and
well-being during pregnancy. This work introduces dynamic electrical
impedance tomography imaging technique for continuous monitoring of
fetus movements. Dynamic electrical impedance tomography (EIT) is
continuous monitoring, a non-invasive, non-ionizing and low cost imaging
modality in which the electrical conductivity or resistivity in the interior of
a pregnant abdomen can be reconstructed by injects sinusoidal electrical
currents to the surface of the pregnant abdomen and collects the voltage
measurements. In this paper EIT forward problem solved by using
boundary element method with (BEM) with complete electrode model
(CEM) and applied smooth variable structure filter (SVSF) method as an
inverse solver to estimate the conductivity distribution inside the pregnant
woman's abdomen. Numerical simulations were performed in Matlab
software and simulation results are accomplished to verify the performance
of the dynamic EIT system.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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B0104
Presentation 8
(15:15~15:30)
Soy Peptone as a Non-Animal Origin N-Source in Salmonella Typhi
Culture Medium For Production of Vi Polysaccharide as a Material of
Typhoid Vaccine
Triana Rahmila, Wardono Niloperbowo and Erman Tritama
School of Life Sciences and Technology – ITB, Indonesia
Abstract—Currently, typhoid vaccine is produced by using a cultivation
media that uses materials originating from animal (animal origin) as one of
its main components. Thus, there is a need for a research about the
production of typhoid vaccine that uses a cultivation media that is free of
animal origin (non-animal origin). This study was done to demonstrate the
correlations between the concentration of capsular polysaccharide Vi, the
antigen source for the production of typhoid vaccine, with the bacterial
strains, medium contents and cultivation methods. The strain that was used
in this study was production strain, Ty2 and patient isolate. One of the
chosen strain candidates was used to compare between the mediums that
used animal origin and non-animal origin in a batch process and fed-batch
process. It was found that the concentration of Vi increased by threefold
when the medium was made using soy peptone (non-animal origin),
instead of casamino acid in a fed-batch process. It was also found that the
optimum glucose concentration of the feed media is 50% of the original
media that was use in the batch phase. The parameters that was used to
analyse this study was optical density at 600nm (OD600), pH, glucose
concentration, and the concentration of Vi that was measured by ELISA.
This study has shown that soy peptone can replace casamino acid as the
component of the medium in the production of capsular polysaccharide Vi.
B0108
Presentation 9
(15:35~15:45)
Reconstruction of Secondary Metabolism of Cordyceps Militaris
Warasinee Mujchariyakul, Chayapat Wizaza and Wanwipa Vongsangnak
Kasetsart University, Thailand
Abstract—Cordyceps militaris is one of entomopathogenic fungi which is
widely used for biometabolites production e.g., cordycepin, adenosine,
cordycepic acid, ergosterol, and polysaccharide. So far, a genome sequence
of C. militaris is available; however there remains largely unknown on
secondary metabolism. Therefore, this study aimed to annotate enzyme
functions for further reconstructing secondary metabolism of C. militaris
using bioinformatics approach. To carry out, we initially used 9,651
predicted protein sequences of C. militaris for enzyme annotation using
EnzDP algorithm. Upon EnzDP together with KEGG database, the results
afterwards showed that 15 secondary metabolic pathways were identified.
Of these pathways, puromycin biosynthesis, indole diterpene alkaloid
biosynthesis, betalain biosynthesis and carbapenem biosynthesis were
considered as the primary results for secondary metabolism reconstruction
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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of C. militaris. The findings provide a basic knowledge in secondary
metabolite biosynthesis in C. militaris.
T0008
Presentation 10
(15:45~16:00)
Synchronization between Respiration and Mastication Functional
Coordination of Respiratory and Digestive Systems
Yutaka Yoshida, Emi Yuda, and Junichiro Hayano
Nagoya City University, Japan
Abstract—The purpose of our study is to evaluate the relationship between
respiratory period and chewing period. In 10 healthy peoples (6 males and
4 females, age 26 ± 7 years), EMG of masticatory muscles and respiratory
curve during chewing of gum were measured and synchronicity between
respiration and chewing were analyzed using the phase of chewing
occurring within respiratory period. Results, the coherence between the
two signals at the peak frequency of respiration was 0.06 ± 0.03 (mean ±
SD). In all subjects, the relationship between respiration period and
chewing period was random and was not correlated. In this experiment,
chewing period wasn’t controlled. Therefore, it is considered that
respiration may have been synchronized if the period of chewing was
controlled.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Session 6 Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session. Afternoon, January 19, 2018 (Friday)
Time: 13:30-16:00
Venue: Room C6 of Global Front (17th Floor)
Topic: “Biomedical Engineering”
Session Chair: To be added
T0005
Presentation 1
(13:30~13:45)
Assessment of Flow Mediated Dilation by Pulse Wave Conduction Delay
Junichiro Hayano, Yutaka Yoshida, and Emi Yuda
Nagoya City University, Japan
Abstract—This study aimed at developing a new method for assessing
flow mediated dilation (FMD) by bilateral differences in pulse conduction
time. Delay in pulse conduction time (PCD) that accompanied FMD
induced by forearm reactive hyperemia with 5-min avascularization was
evaluated. PCD increased with FMD but the correlation coefficient
between PCD and FMD was 0.33. PCD may have been influenced by
unmeasured factors other than FMD.
B0018
Presentation 2
(13:45~14:00)
Added Force-Based Methods Using Load Cells to Evaluate Walking and
Tumbling with a Japanese Walking Support System
Shinji Kawakura and Kiyoshi Fujiwara
National Institute of Advanced Industrial Science and Technology (AIST),
Japan
Abstract—We have conducted sequential studies to develop promising
systems that address nursing challenges, and have developed applied
sensing systems to solve the difficulties faced to advanced support systems
for physically challenged and elderly people. In this study, our main aim
was to present the utilities of the immediate and distant measurement
systems by presenting qualitative and quantitative timeline data from one
user of a typical Japanese walking support System. We suggested a
physical analysis system using four bar-shaped metal load cells, Arduino
microcomputers, and set specialized integrating and amplifying circuit
boards on the arm rest to obtain data of the added weight of users on the
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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front point of the machine. Additionally, we developed a physical analysis
system, which uses two video cameras to obtain visual data of a subject
simulating using programs written in Visual C++ and OpenCV packages,
including the libraries and header files. Finally, we presented the multiple
output values of the physical measurements obtained from various
viewpoints, and examined the characters of timeline data and their
kinematic parameters. We believe these mixed methodologies could be of
practical use in providing alternative supports and useful directions for
developers and care managers to assess and treat users’ conditions.
B0035
Presentation 3
(14:00~14:15)
Optimal-channel Selection Algorithms in Mental Tasks based
Brain-computer Interface
Han Sun, Bruce Gluckman, Xiong Zhang, Yu Zhang, Xuefei Zhong and
Zhaowen Fan
Southeast University, China
Abstract—Brain computer interface (BCI) for healthy people is a growing
field. Minimizing the number of electroencephalography (EEG) channels is
a key technological advantage for the application of BCI, which would
make the system more mobile, easier to setup and long-time use in the real
life. In this paper, to decrease the number of channels, multi-channel
common spatial pattern (MCSP) algorithm is used to extract the features
with two mental tasks (i.e., mental arithmetic and spatial imagery), and
support vector machine (SVM) is used to classify the tasks performed. In
detail, the separability value of each individual channel is computed based
on between/within-group variance and a modified entropy criterion to
evaluate its contribution to classification performance. The optimal
channels are chosen based on the separability ranking. The performance of
proposed methods is compared with recursive channel elimination and
genetic algorithm. The results demonstrate that the EEG signals have
different trends between the two mental tasks with highest brain activity in
left central-parietal and parietal lobes, and the separability values allow
reduction of number of electrodes from 15 to 4 and 10 while the
classification accuracy reaches 80% and 90% respectively. Therefore, the
optimal-channel algorithms can reduce the number of channels and
improve the performance of the mental tasks based BCI.
B0044
Presentation 4
(14:15~14:30)
Development of Portable and Wireless Closed-loop Systems for
Responsive Neurostimulation and Neurofeedback Training in Animals
Sheng-Fu Liang, Yu-Hsuan Shih, Wen-Hsiu Yeh and Fu-Zen Shaw
National Cheng Kung University, Taiwan
Abstract—Advances in electronic and information technologies support the
development of many new diagnostics and treatments for healthcare. A
closed-loop platform that can perform on-line event detection and real-time
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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stimulation or feedback such as responsive neurostimulators and
neurofeedback training (NFT) systems will perform alternative treatments
in addition to pharmacological therapy. A closed-loop seizure controller
that can perform on-line seizure detection and real-time electrical
stimulation for seizure elimination is developed and applied to Long-Evans
rats with spontaneous absence epilepsy. The seizure detection accuracy is
greater than 92% and the averaged seizure detection latency is less than 1
s. 90% of the absence seizures can be suppressed by a 0.5-s 800 Hz
electrical stimulation on zona incerta. The wireless EEG monitoring
module and a LabVIEW interface are also integrated with a Skinner box to
perform neurofeedback training. If the power of alpha rhythm of an animal
is sustained and higher than the threshold, the food is given as a reward.
This NFT system can successfully train the Sprague Dawley rats to have a
progressive significant increase in the alpha amplitude and total alpha
duration in the 12 training sessions. The developed systems can also be
applied to various researches in bioelectronics, medical informatics and
neuroscience in the future.
B3012
Presentation 5
(14:30~14:45)
Effective Decolorization of Malachite Green Using Kocuria Marina
DAGII and Its Toxicological Study
Tonmoy Ray, Daiji Brahma, Ruchira Mitra and Debjani Dutta
National Institute of Technology, Durgapur, India
Abstract—Malachite Green (MG) is a highly toxic synthetic dye which is a
major component of textile industry. It alone accounts for the two-third of
the total production of the wastewater. Biological decolorization of dye is
highly effective and a promising method due to its low cost and
environmental friendly approach. The present study involves efficient
detoxification of Malachite Green by the bacterium Kocuria marina DAG
II through batch process. It was observed that more than 99 % dye removal
was achieved within 8 hours at 28°C with an initial MG concentration of
13mg/L, initial pH of 7.2 and an inoculum size of 1.2% (v/v). Microbial
toxicity test was conducted against Bradyrhizobium sp. using Agar well
diffusion method. It was inferred that the treated effluent had no zone of
inhibition but on contrary the untreated MG solution exhibited 35 ±5 mm
inhibition zone in agar plates. Additionally the toxicity of the treated dye
solution was checked on the seeds of Vigna radiata (Indian mung bean), a
major cultivated Indian pulse crop. Though the phytotoxicity results
revealed 100% germination in distilled water (positive control), untreated
and treated dye solution, however the root and the shoot length were
negligible in case of untreated dye solution. In contrast, the root and shoot
length for treated MG solution was similar to the distilled water. Thus,
the toxicological studies indicated that the treated dye solution was
appreciably less toxic compared to untreated MG solution.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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T0015
Presentation 6
(14:45~15:00)
Wii Balanced Board-based Utilization for The Human Body Center of
Pressure for the Falling Detection via Multivariate Empirical Mode
Decomposition and Multivariate Multi-Scale Entropy
Jiann-Shing Shieh, Ting-Hui Li, Chih-Dao Chen, and Maysam F. Abbod
Yuan Ze University, Taiwan
Abstract—Center of pressure (COP) evaluation is one of important
parameters in the fall detection algorithm for the elder people. The Wii
balance board (WBB) (Nintendo Co., Ltd., Redmond, WA 98052),
originally an accessory of a game controller for the Wii video game
console, is a potential device that can be utilized for the human balance
evaluation system. Initially, this study evaluates the WBB signal in
comparison to the AMTI (Advanced Mechanical Technology Inc., Newton,
MA, USA) as the reference. Initially, the experiment is conducted for three
different conditions; open eye, close eye and one-foot stand for five
volunteers. The mean of correlation coefficient between WBB and AMTI
is over 0.98 on X-direction and over 0.99 on Y-direction. The multivariate
multi-scale entropy (MMSE) and multivariate empirical mode
decomposition (MEMD) are used for the signal feature evaluations. The
results show that complexity indexes (CI) from the MSE-based algorithms
have significant results only between young and elderly groups and young
and elderly-fall groups. However, there are no significant differences on
between elderly and elderly-fall groups. In conclusion, this study shows
that the WBB can be applied as the balance-measuring device. Meanwhile,
further investigations need to be conducted for the advanced algorithms to
fall detection for the elderly people.
B0040
Presentation 7
(15:00~15:15)
A Wearable Wireless fNIRS System
Yu Zhang, Xiong Zhang, Han Sun, Xuefei Zhong and Zhaowen Fan
Southeast University, China
Abstract—Neurovascular coupling mechanisms in functional brain activity
has always been important areas of brain science. Functional near-infrared
spectroscopy (fNIRS) has been utilized in the recent decades for examining
functional brain activity by measuring the changes in the concentrations of
oxygenated and deoxygenated hemoglobin. fNIRS is a noninvasive
detecting technology, it has many characteristics such as high measurement
speed, simple operation, fNIRS represents a good compromise in terms of
spatial and temporal resolution, and so it’s becoming more and more
widely used. But recent fNIRS still non-wearable and participants are
nonmoving.
In this work, we design a low-cost, low power, wearable and wireless
fNIRS system. This system has two wavelengths light of 760nm and
850nm, and transfer data via Bluetooth. We design and make a plastic
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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housing for fix the optical fiber by 3D printer. The battery can last several
hours. In an experiment, this system can detect change of Hb and HbO2’s
concentration.
B0003
Presentation 8
(15:15~15:30)
Preparation of an Electrically Conductive Graphene Oxide/Chitosan
Scaffold for Cardiac Tissue Engineering
Lili Jiang, Daoyu Chen, Zhongmin Zhang, Yangliu Xia, Hongyu Xue and
Yong Liu
Dalian University of Technology Panjin, China
Abstract—Cardiac tissue engineering is thought to be of great importance
for therapeutic and pharmaceutical applications. The scaffolds that can
provide electrical conductivity and structural organization would be highly
beneficial for cardiac tissue engineering. Here, we developed conductive
scaffolds with electrical conductivity and porous structure composed of
chitosan (CS) blending with graphene oxide (GO) for cardiac tissue
engineering. Our results showed that the swelling, porosity, and conductive
properties of GO/CS scaffolds could be modulated via adjusting the ratio
of graphene oxide to chitosan. More importantly, GO/CS scaffolds had
swelling ratio ranging from 23.20 to 27.38 (1000%) and their conductivity
(≈0.7×10-5 S·cm -1) fell in the range of reported conductivities for native
cardiac tissue. Furthermore, we assessed their biological activity by
seeding H9C2 cells in GO/CS scaffolds. Our data showed that these
GO/CS scaffolds exhibited good cell viability, promoted cell attachment
and intercellular network formation, upregulated expression of the cardiac
specific gene and protein involved in muscle conduction of electrical
signals (connexin-43). Overall, it is concluded that the GO/CS scaffolds
promoted the properties of cardiac tissue constructs, demonstrated an ideal
scaffold for cardiac tissue engineering.
B0031
Presentation 9
(15:35~15:45)
Measurement and Evaluation of the Bioelectrical Impedance of a Rubber
Humanoid Phantom Wrapped with Gel Sheets
Shinji Kawakura, Yoshihiro Nakabo and Kiyoshi Fujiwara
National Institute of Advanced Industrial Science and Technology (AIST),
Japan
Abstract—This study aimed to estimate the impedance values of an
originally made, artificial rubber phantom attached with one elastic gel
sheet using Physion MD (Doctor of Medicine). The electromagnetic
characteristics of the material were similar to human body. In previous
studies, specially designed bioelectrical impedance acquisition systems and
MRI (magnetic resonance imaging) methods were used to independently
and repetitively measure bioelectrical impedance and serial cross-sectional
images of the trunk, forearms, upper arms, lower legs, and thighs of
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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healthy adult men. Bioelectrical impedance was significantly correlated to
muscle and fat volumes measured by MRI. Based on this relationship, the
estimated values of muscle and fat could also be calculated from the
bioelectrical impedance. However, past methodologies have various
limitations. In this current, subsequent research, only impedance values
were measured, while varying superficial variables (e.g., attaching gel
sheets, changing electrode types, and changing the insertion depth of
needle-shaped electrodes) This present study indicates that bioelectrical
impedance analysis for this rubber phantom including the version with
specific gel sheets attached and the version containing normal saline
solution is a promising physical simulator, and may be useful for the
prediction of not only virtual muscle volume, however, also diverse inner
body conditions.
B0093
Presentation 10
(15:45~16:00)
Preparation of Calcium Phosphate Biocomposite and the Study of Bone
Formation in Mice
Lijia Cheng, Jianhong Yang, Tao Chen, Xinrong Xie and Zheng Shi
Chengdu University, China
Abstract—Objective: To prepare a type of thermos-sensitive
hydrogel/tricalcium phosphate (TSH/TCP) biocomposite, and investigate
its osteogenic ability. Methods: The TSH was dissolved at 4℃, then the
TCP powder was added into TSH at a ratio of 24/76, and the
24TSH/76TCP biocomposite was prepared above 27 ℃ . Next, the
composite materials, hydroxyapatite (HA) and β-tricalcium phosphate
(β-TCP) were implanted into the thigh muscles of BALB/c mice; twelve
weeks after the operation, three types of materials were harvested, fixed
and paraffin embedded, then the sections were performed histological
staining to observe the new bone formation. Results: At 12 weeks, a large
number of new bone tissues were detected in both TSH/TCP and β-TCP
materials with different formation mode; however, there wasn’t any bone
tissues in HA. Conclusion: TSH/TCP is a biocomposite which can be
shaped into any shape according to different needs, and it has strong
osteogenic ability in mice.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Session 7 Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session. Afternoon, January 19, 2018 (Friday)
Time: 13:30-16:00
Venue: Conference Room 1 of University Hall (3rd Floor )
Topic: “Microorganism and Ecosystem”
Session Chair: Assoc. Prof. Siew Woh Choo
T0003
Presentation 1
(13:30~13:45)
Deforestation across the World: Causes and Alternatives for Mitigating
Alexandre Marco da Silva and John Rodgers
São Paulo State University, Brazil
Abstract - We underline the main causes of deforestation and present a
framework for mitigating the continual loss of forest habitat around the
world. The causes of deforestation are complex and they operate at
multiple spatial and temporal scales, yet they are related to societal and
political factors. Societal factors consist of human migration within rural
settings and poverty. Political factors include ineffective land management
and negligence of monitoring and protecting forest commodities.
Combined, these factors present arduous challenges to protecting forest
habitats. In turn, there are at least three strategies to reduce further forest
loss. The first strategy involves the cessation of timber cutting and
extraction of other forest products. But just as important is the second
strategy of creating and maintaining more Environmental Protected Areas.
The third strategy is perhaps the greatest challenge, and it involves the
adoption of the “green nudge principle”, whereby individuals are educated
to modify individual behaviors related to patterns of consumption and to
the conservation of remaining forests. This is especially true for the
increasingly endangered primary forests. An adoption of these mitigation
strategies will help reduce deforestation and will help improve global
environments.
T0006
Presentation 2
(13:45~14:00)
Developing Ecosystem Accounts as Basic Information System for
Monitoring the Changes of Peatland Ecosystem in Indonesia
Resti Salmayenti
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Wageningen University and Research, The Netherlands
Abstract—Indonesian peatlands only cover 8% of Indonesian surface but
provide enormous benefits for human throughout agricultural sector.
However, activities in peatlands lead to various environmental impacts like
land degradation. Plenty studies about peatlands have been conducted,
however, available information is not developed in an integrating system
for giving insight the changes of environmental aspects and economic
activities. This research applies SEEA-EEA framework to monitor the
ecosystem changes concerning their physical and monetary values. This
study starts indicating the changes of eight-peatland cover (1990-2014) in
extent account, 52% of forest area in 1990 have been converted to other
land uses. Next, ecosystem condition account explains the changes of
environmental states using four indicators (precipitation, burned area,
degraded land and forest biomass). Third, ES account indicates seven-main
ecosystem services (oil palm, biomass for pulp, paddy, timber,
CO2-sequestration, biodiversity habitat and nature watching). These
services are worth around €5.5-billion (2000) and increased to €7-billion
(2014). Oil palm production has been expanded significantly, contributed
to 49% of total value in 2014. Carbon account shows that CO2-emission
highly increased from 210Mt-CO2/year (€5.8-billion) (2000) to
385Mt-CO2/year (€10.8-billion) (2014). Lastly, ecosystem asset presents
the estimated NPVs of paddy field, oil palm and acacia plantation in
20-year period.
T0014
Presentation 3
(14:00~14:15)
Investigation on Thermochemical Behavior of Oil Palm Residues and their
Bio-Oil from Pyrolysis Process
Yanisa Chantanumat
Prince of Songkla University, Thailand
Abstract—The palm oil industry generates an abundance of oil palm
residues namely empty fruit bunch, mesocarp fiber and kernel shell. These
residues are one of the biomass resources that can be utilized for the
production of bio-oil or chemicals via the pyrolysis process. Thermal
behavior of palm empty fruit bunches (EFB), palm mesocarp fiber (PF)
and palm kernel shell (PKS) was investigated using thermogravimetric
analysis (TGA) conducted under N2 atmosphere at a heating rate of
10°C/min from 50 to 600°C. Palm empty fruit bunches, mesocarp fiber
and kernel shell were pyrolyzed in a fixed bed reactor at 500 °C for 60 min
under the flow rate 400 mL/min of N2 as carrier gas. The chemical
composition of bio-oil was analyzed by a gas chromatography/mass
spectrometry (GC/MS). The thermal profiles of the three residues appeared
to correlate with the cellulose, hemicellulose and lignin content in the
samples. A great range of chemicals such as hydrocarbons, ketones,
aldehydes, acids, phenols and nitrogen compounds were indicated in PKS,
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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EFB and PF bio-oil. Pyrolysis oil obtained from PKS, EFB and PF has the
potential to be utilized as chemical feedstocks or possibly upgraded for
fuels.
T0021
Presentation 4
(14:15~14:30)
Landscape capacity for supplying ecosystem services in Taiwan Alishan
National Scenic Area
Fu-Chiang Yang and Yi-Tui Chen
National Taipei University of Nursing and Health Sciences, Taiwan
Abstract—For some service facilities, the unused service capacity cannot
be stocked overnight and will become a burden to the service provider. The
main purpose of this article is to analyze the effects of seasonality on
capacity choice decisions and examine the firm’s pricing strategies. We
present a mathematical model and find that higher operating costs lead to
lower service fees in booming seasons and higher service fee in normal
seasons, but the capacity should be decreased; the higher proportion of
booming seasons in a year results in higher service fee in booming seasons
and lower service fee in normal seasons, and the capacity should be
increased. The price markup in booming seasons is higher if the
proportion of booming seasons increase, but lower if operating costs
increase.
T0011
Presentation 5
(14:30~14:45)
Optimization of indole-3-acetic acid production by diazotrophic B.subtilis
(KP455653), isolated from rhizosphere of Eragrostis cynosuroides
Sonali Kumari, Chander Prabha, Abha Singh, Sushma Kumari, and
Shilpi Kiran
Patna University, India
Abstract—Diazotrophic rhizobacteria, trigger and enhance plant growth as
well as yield through various mechanisms, so their use can reduce the
application frequency of chemical fertilizers. Indole-3-acetic acid (IAA), a
most common natural auxin influences several physiological processes of
the plant’s health. The present study is aimed to optimize the conditions for
IAA production, along with assay for plant growth promoting traits of
Bacillus subtilis DR2 (KP455653), which is a diazotrophic Gram positive,
rod bacterium, isolated from rhizosphere of road side weed, Eragrostis
cynosuroides from Danapur, Patna, Bihar, India. The screening for IAA
production was done in JNFbˉ broth with tryptophan (1 g.l-1
) and without
tryptophan at pH 5.8, 30±2 °C temperature and 48 h incubation. 137.81
µg.ml-1
and 100.26 µg.ml-1
IAA was produced in Trp+ and Trp
- media,
respectively. Under various optimized conditions, maximum IAA was
produced at 96 h incubation (137.81 µg.ml-1
), 35 °C temperature (141.92
µg.ml-1
), pH 7 (158.79 µg.ml-1
), mannitol as carbon (160.85 µg.ml-1
) and
ammonium sulfate as nitrogen (162.93 µg.ml-1
) sources with tryptophan at
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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final concentration of 1.2 µg.ml-1
(168.09 µg.ml-1
), which enhanced the
production by 1.2 fold. The findings suggest that B. subtilis DR2 is a
potent organism to be used as biofertilizer.
T0013
Presentation 6
(14:45~15:00)
Isolation and Characterization of thermostable amylase producing bacteria
from hot springs of Bihar, India
Shilpi Kiran, Abha Singh, Cahander Prabha, Sushma Kumari, and Sonali
Kumari
Patna University, India
Abstract—Amylases are starch degrading enzyme with wide industrial
applications. Microbial production of amylase is more simplified and
economical than other sources. In this study, amylase producing bacteria
were isolated from hot springs of Munger (Bihar, India). Water samples
were collected from three different kunds, viz., Sita kund, Rishi kund and
Bhimbandh kund with temperature 40-50°C and pH 5.2-6.3. Amongst
amylase positive isolates, RK6 was selected for amylase characterization,
exhibiting enzyme activity at highest optimum temperature of 80°C. On the
basis of 16S rRNA gene sequence, it was identified as B. subtilis RK6
(KX247637). Amylase of RK6 was characterized with optimum pH of 8.0
and was thermoactive, retaining its activity upto 90°C and stability at
60-90°C after preheating for 30 min. Vmax and Km for enzyme activity were
60.56 U/ml and 1.86 mg/ml respectively, showing an appreciable affinity
for substrate. The enzyme was activated in the presence of Ca2+
, Mg2+
, and
Fe2+
, while that of Zn2+
and Cu2+
resulted in its inhibition. The present
finding indicates potential of thermo-alkaliphilic amylase of B. subtilis
RK6 for various biotechnological applications.
T2005
Presentation 7
(15:00~15:15)
Evaluating Hydrophobic Bacteria as a Potential Adsorbent for Oestrogen
Removal in Wastewater Treatment
Nur Nasimi Abdul Latif, Dr. Paul Sallis and Dr. Russell Davenport
Newcastle University, United Kingdom
Abstract—Oestrogens are endocrine disrupting compounds known to
produce an adverse effect on the aquatic population by altering their
reproductive functions, even at very low concentration i.e. part per trillion.
This study makes use of the hydrophobicity and obtains highly
hydrophobic bacterial strains that can adsorb oestrogens to a level that has
no health and ecological implications. Strains of Rhodococcus erythropolis
were adapted using hydrocarbons and new bacterial species were isolated
from a diesel-contaminated soil through soil-enrichment. N-hexadecane
and hexane (hydrocarbons) were used as substrates.
This study shows that the Rh. Erythropolis DSM311 adapted in
n-hexadecane had 6.7% Cell Surface Hydrophobicity (CSH) increment
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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compare to its parent strain and the soil-isolated SD2-1 strain showed
91.33% CSH. Moreover, the stationary phase bacteria (collected at day 10
incubation) were found to have a higher CSH and produced higher
oestrogen removal compared to the exponential phase cell (collected at day
5). An optimization study revealed that 50mg of SD2-1 strain collected at
the stationary phase was found to be the optimum condition for 95.3%
oestrogen removal. This performance was almost comparable to the
activated carbon adsorbence. In conclusion, higher CSH bacterial strains
adapted with hydrocarbon are more efficient in removing oestrogen
compared to the low CSH strains.
T1002
Presentation 8
(15:15~15:30)
Enhanced Phytoextraction of Cadmium by Dianthus Chinensis with
Biodegradable Chelators for Soil Remediation
Erh-Hsuan Tsai and Zeng-Yei Hseu
National Taiwan University, Taiwan
Abstract—Phytoextraction is a technique by harvesting the plants which
can remove contaminants from soils. Different chelators have been used to
increase the bioavailability of heavy metals and enhance the efficiency of
phytoremediation. Rainbow pink (Dianthus chinensis Linn.) has been
proved to accumulate a high amount of Cd from soil, and thus this study
explored the Cd accumulation in Dianthus chinensis treated with different
biodegradable chelators in Cd contaminated soils (10 and 20 mg Cd/kg) for
50 days. The tested chelators were N,N'-disuccinic acid (EDDS),
3-Hydroxy-2,2'-Iminodisuccinate (HIDS), citric acid (CA), and succinic
acid (SA). The experimental results indicated that the soluble Cd
concentration was the highest in the EDDS treatment and up to 367 μg/L,
followed by HIDS, CA, and SA in the 10 mg Cd/kg soil. However, all
chelator treatments were significantly higher than control. In the 20 mg
Cd/kg soil, the soluble Cd concentration in all chelator treatments exceeded
1000 μg/L. There was no significant (P < 0.05) difference on plant weight,
chlorophyll and proline contents, and the reactivity of triphenyltetrazolium
chloride (TTC) in roots in all chelator treatments, but different efficiency
of Cd uptake existed among them. The maximum Cd concentration in the
shoot was 52.8 mg/kg in the EDDS treatment, followed by CA, SA and
HIDS treatment, while the control was only 31.7 mg/kg. The results shows
that the addition of biodegradable chelators can be used for the enhanced
phytoextraction of Cd and EDDS is the best chelator in this study.
T0004
Presentation 9
(15:30~15:45)
Synthesis and Water-Repelling Properties of New Acrylates with Short
Fluorinated Alkyl Chains
Eun-kyung Kang, Eun-Ho Sohn, and Byung Min Lee
University of Science and Technology, Korea
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Abstract—Fluorine compounds which are stable to high temperature,
strong acid and base have been widely used in many fields because of their
excellent surface properties. Fluorinated compounds with long
fluoro-carbon chains, especially perfluorooctanoic acid (PFOA) and its
derivatives, are harmful to human health and the environment. The use of
long perfluoroalkyl compounds such as perfluorooctanoic acid (PFOA)
was restricted owing to their non-biodegradable and toxic effects in the
environment. In order to solve these problems, we were investigated the
new repellents having short fluorinated alkyl chains. The structures of
designed products were identified by NMR and FT-IR. Water repellency of
synthetic water-repellent agents was confirmed by measuring the water
contact angle.
T0023
Presentation
10
(15:45~16:00)
Adsorption of Fe Ions from Aqueous Solution Using α-keratin-coated
Alginate Biosorbent
Ganjar Fadillah, Elsa Ninda Karlinda Putri, Syahna Febrianastuti, Easy
Vicky Maylinda, and Candra Purnawan
Institute of Technology Bandung, Indonesia
Abstract—The biosorbent α-keratin-alginate (KA) was prepared by using
the encapsulation technique in CaCl2 2 % (w/v) solution. The biosorbent
was characterized by Fourier Transform Infrared (FTIR) and Scanning
Electron Microscope (SEM). The extent of adsorption was found to be a
function of the composition of α-keratin and alginate, the pH of solution
and contact time. The optimum adsorption of Fe ions in aqueous solution
was found at the composition of α-keratin and alginate of 1:2 (w/w), the
pH at 7.0 and contact time at 60 minutes. The adsorption of Fe ions on KA
biosorbent was comparatively higher than α-keratin and alginate only. The
adsorption capacity of KA biosorbent has the maximum adsorption
capacity of 658.4 mg/g while biosorbent α-keratin and alginate are 464.7
mg/g and 528.1 mg/g, respectively. Adsorption of Fe ions in aqueous
solution followed the Freundlich adsorption isotherm, and the dynamic
adsorption model could be described through a pseudo-second order
kinetics.
Coffee Break
16:00~16:20 Lounge in Front of C5
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Session 8 Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session. Afternoon, January 19, 2018 (Friday)
Time: 16:20~18:50
Venue: Room C5 of Global Front (17th Floor)
Topic: “Gene Expression and Analysis”
Session Chair: Prof. Egils Stalidzans
B0081
Presentation 1
(16:20~16:35)
Comparative Co-expression Analysis between Soybean and Arabidopsis
Zhixing Zhu, Jing Peng, Yuhua Fu, Yangjing Gan and Daipeng Luo
Wuhan University of Technology, China
Abstract—Arabidopsis thaliana, an important model species, is widely
used in biology research, and employing its knowledge to study other
related species is one of the major applications. Soybean is a major legume
crop and it is of great economic and nutritional value. Therefore, many
co-expression databases including these two species are constructed. But,
there is little research focused on the differences of their orthologous gene
co-expression relationship. In this paper, we calculated the general
co-expression coefficient of any two genes in orthologous genes, compared
the differences of this coefficient between Arabidopsis and Soybean, and
analyzed the similarity of these two species. Although many studies on
Soybean are based on the orthologous relationship with Arabidopsis, our
results show that there only about 38.85% gene pairs are similar between
the two species. Our study indicates that researchers should have an eye on
these existed differences when conducting research on Arabidopsis and
Soybean.
B0086
Presentation 2
(16:35~16:50)
Genome Scale Metabolic Model of Arabidopsis Thalina for Isoprenoid
Production
Agris Pentjušs and Egils Stalidzans
Latvia University of Agriculture, Latvia
Abstract—Capacity of plants of converting light to different valuable
products makes them highly relevant to “green factories”. To reduce
burden of arable land usage for valuable product production there is need
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to increase significant plant productivity.
In this study to we use Arabidopsis thaliana genome scale model in a
synthetic biology based approach to increase production of z-abienol and
isoprene depending on light intensity and diurnal cycle. Genome scale
model is primarily derived from annotation aracyc 13.0 in the Aracyc
database. We are using linear programming to demonstrate model
capability to produce biomass components (amino acids, nucleotides,
lipids, starch, cellulose). Model structure uses cytosol, mitochondria,
plastid, vacuole and peroxisome compartments. Compartmented reactions
are mostly used for interaction analysis of light and diurnal cycle involved
reactions.
Kinetic model of MEP as plant isoprenyl precursor pathway located in
plastids will be used in combination with genome scale model to assess the
feasibility of kinetic model steady states suggested by optimizations.
B0088
Presentation 3
(16:50~17:05)
Molecular Cloning and Comparative Analysis of Variable Regions of
Monoclonal Antibody against Enrofloxacin Clone 48
Jirawat Mala, Songchan Puthong, Kittinan Komolpis and Sarintip
Sooksai
Suratthani Rajabhat University, Thailand
Abstract—Enrofloxacin is fluoroquinolone antibiotic which prohibited
approved for treatment in animals. However, their residues in animal
products can pose adverse side effects to consumer. Therefore, the
maximum residue limit of these drugs has been enforced in many
countries. In this study, the cDNA encoding VH and VL genes was
amplified from monoclonal antibody which was specific to enrofloxacin
clone 48, cloned and sequenced. The obtained sequences were compared in
the NCBI databases by using blastp program. The results found that VH
nucleotide was composed of about 399 bps and theirs deduced amino acids
showed 80-85% degree identities to the Ig superfamily group. A totally 356
bps of VL nucleotide was found and showed the degree of identities of
97-100% with an immunoglobulin kappa light chain. Moreover, the CDR I,
CDR II and CDR III of the VH and VL sequences were specified. The
results indicated that the highest degree of VH sequence homology (highest
relationship) found in accession no. B26471 with 80% homology.
However, 100% of VL sequences were found similarly to accession no.
AHJ10945.1 than the others. The obtained results provided the useful and
important information for the further recombinant antibody construction
and production against enrofloxacin antibiotic.
B0114
Presentation 4
Feasibility of Using Hypersaline Lake Sediment as Inoculum for Biogas
Production from Anaerobic Digestion of Saline Wastewater
Manal Ali, Ahmed Elreedy and Ahmed Tawfik
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(17:05~17:20)
Egypt-Japan university of science and technology (E-JUST), Egypt
Abstract—Anaerobic digestion processes have been widely used for biogas
production from various substrates, however, without much attention to the
saline effluents containing organic compounds. This study aims to
investigate the use of hypersaline lake sediment as inoculum, after a
hierarchical acclimation process, at different inoculum-to-substrate ratios
(ISRs) and salt (NaCl) concentrations. Batch-mode experiments were
conducted to achieve this goal using glucose as a substrate. ISRs ranging
from 11.5 to 28.74 gVSS/gCOD and NaCl concentrations of 20-30 g/l were
tested. Maximum cumulative biogas production (CBP) of 55 ml was
achieved at ISR of 11.49 gVSS/gCOD and NaCl concentration of 30 g/l,
resulting a biogas production rate (BPR) and specific biogas production
(SBP) of 1.08 ml/h and 220 ml/l, respectively. Moreover, at same
conditions, the highest chemical oxygen demand (COD) removal
efficiency of 84.12 % was resulted. The highest acidification corresponded
by maximum production of volatile fatty acids (VFAs) of 1652.2 mg/L was
found at ISR of 11.5 gVSS/gCOD. The acetic acid was predominant with a
maximum concentration of 500 mg/L. A modified Gompertz equation
successfully described the time-course cumulative biogas production (R2 =
0.989). Eventually, at optimum conditions, the microbial enriched
sediment collected from hypersaline lakes proved its easy and effective use
as inoculum for biogas production from saline wastewater.
B0076
Presentation 5
(17:20~17:35)
Novel Strategy to Quantify Circular RNA Using Model-Based Framework
Musheng Li, Tong Zhou and Wanjun Gu
Southeast University, China
Abstract—Circular RNA is a class of widely expressed non-coding RNA
whose both ends covalently bond together to form a circular shape. Some
of them are reported to work as miRNA sponge such as CDR1as. but the
exact functionality of most circular RNA is still unknown. To gain more
understanding of their functionality. circular RNA transcript abundance
in RNA-seq datasets should be estimated. but existing state-of-art
transcript expression quantification tools are not suitable for circular RNA.
Here, we present a strategy that transform circular transcripts into
pseudo-linear transcript and estimates the expression values of both
circular and linear transcripts using existing model-based algorithm named
Sailfish. We test this strategy in both simulated and real rRNA depleted
RNA-seq data. Result shows this new strategy can estimate both linear and
circular transcript accurately. It gives superior performance on compare to
traditional count-based method. Our result also shows that by taking
circular RNA into consideration will increase accuracy of linear transcript
expression when estimating transcript abundance in rRNA depleted
RNA-seq dataset.
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B0019
Presentation 6
(17:35~17:50)
Preliminary Genetic Assessment OF Elephas maximus by Using Fecal
Samples from Selected Hotspots in Taman Negara National Parks,
Peninsular Malaysia
Kayal Vizi Karuppannan, Badrul Munir Md Zain, Salmah Yaakop and
Jesús E Maldonado
Department of Wildlife and National Parks (Dwnp) Peninsular Malaysia,
Malaysia
Abstract—One of the main goals in National Elephant Conservation Action
Plan (NECAP) is genetic assessment of Asian elephant in Taman Negara
National Parks, Peninsular Malaysia. As a first step, we examined a total of
38 Elephas maximus fecal samples using microsatellite, molecular sexing
and control region markers to gather all available genetic information.
Twenty eight fecal samples were collected from selected locations in
Taman Negara National Parks and remaining positive control samples were
from known male and female elephants of National Elephant Conservation
Centre (NECC). We use 15 pairs of fluorescently labeled polymorphic
microsatellite and 3 pairs of molecular sexing markers together with
mtDNA marker in this study. Laboratory process were started from DNA
extraction, PCR and completed by fragment analysis. Several analysis
programmes were used in interpreting data and standard genetic
assessment including sex ratio were calculated. The final outcomes of
this experiment will provide information on genotypes and sex ratio of
wild E.maximus in the study area and can be extended for other collected
samples to get a complete database on elephant population structure in
Taman Negara National Parks.
B0115
Presentation 7
(17:50~18:05)
Lymphoma-Associated Gene Expression Changes in an Inducible Model of
Myc-Driven B-Cell Lymphoma
Amir Mahani, Gustav Advirson, Alf Grandien and Anthony P. H. Wright
Karolinska Institute , Huddinge, Sweden
Abstract—The c-Myc transcription factor, identified as a proto-oncogene,
is involved in human malignancies. In Burkitt’s-Lymphoma (BL),
translocation of c-Myc (MycWT) plays a major role in lymphoma
development. In addition, mutations causing substitutions in c-Myc are
associated with BL. Interestingly, the MycT58A and MycT58I mutations
were reported to increase cellular transformation and stability of the Myc
protein. To understand the role of MycWT and associated-BL mutations in
lymphoma, we made three different B-cell lines that express MycWT,
MycT58A, or MycT58I in a doxycycline dose dependent manner. At high
levels of Myc the cell lines display characteristics associated with
lymphoma cells. In this study, we overexpressed Myc at seven different
levels in each cell line and identified cell cycle changes by flow-cytometry
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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using propidium iodide staining. Changes in cell size and number where
also measured. mRNA was sent for NGS sequencing. Myc dependent
changes in RNAseq data were analysed using generalized linear models to
identify and compare different regulatory patterns (maSigPro package)
associated with the wild type and mutant Myc proteins. GO terms could be
mapped to gene clusters with different responses to Myc and can explain
important changes that occur in lymphomas that are driven by wild type or
mutant Myc.
B0036
Presentation 8
(18:05~18:20)
Use of Low Temperature Cold Atmospheric Plasma in The Treatment of
Melanoma Cells
Pei-Ru Chen, Ming-Chen Wang and Yun-Ju Chuang
Ming Chuan University, Taiwan
Abstract—Recently, cold atmospheric-pressure (CAP) plasma have
become a novel and popular tools in biomedical applications. It has been
proved that CAP plasma treatment will induce cancer cells death. With
different CAP plasma treatment parameters, cells will have different fate
including proliferation, apoptosis and cell membrane damage. It has been
reported that a low energy plasma will cause cell proliferation while a
longer exposures lead to cell death. So, the aim for this study is to induce
melanoma cells apoptosis in a short time by CAP plasma-jet. We first
evaluate the optimal working condition of CAP plasma-jet including
treatment time, voltage, flow-rate and plasma-gas composition. Then we
use CAP plasma-jet treat on melanoma cells with different medium
volume, cell number and treating times to find a suitable condition for the
cell culture. Results demonstrate that with medium 0.6 ml and seeding 104
cells have highest cell viability while treat 180 s and 360 s are significantly
decreased. In the migration assay, cells migration ability is both
decreased when treat with plasma for 180 s and 360 s. We also do the
Hoechst 33258 stain, after treating with CAP plasma melanoma cells are
detach from the bottom and have DNA condensation. Finally, we
examine ROS expression present/ absent ROS inhibitor sodium pyruvate
(SP). Data shows that after CAP plasma treatment ROS expression is
induced while cell viability is reduced. But cell viability is recovered by
pretreat with ROS inhibitor SP. In conclusion, both cell viability and cell
migration ability are inhibited by CAP plasma-jet via ROS mediation in
melanoma cells.
B0099
Presentation 9
(18:20~18:35)
A Study of Immune Function Improvement Induced by Ganoderma
Lucidum Alkaloid in Mice
Lijia Cheng, Zheng Shi and Tian Yu
Chengdu University, China
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Abstract—Objective: To study the effect of extractive of Ganoderma
lucidum (G. lucidum) alkaloid on immune function in mice. Methods: 48
female BALB/c mice were randomly divided into 4 groups (n=12), 10
mg/kgd (group A), 50 mg/kgd (group B), 100 mg/kgd (group C) and the
control group (group D). All animals were administered by gavage for 14
days. At last, macrophage phagocytosis ability, T lymphocyte
transformation capacity and interferon-gamma (IFN-γ) levels were
measured. Results: The G. lucidum alkaloids could significantly promote
the spleen/body weight ratio, the proliferation of T lymphocytes, the
macrophage activity and the levels of IFN-γ in BALB/c mice by oral
treatment. Conclusion: G. lucidum alkaloid could improve the immune
function of mice, suggesting that G. lucidum alkaloids is a promising
candidate for promoting immune response and a potentially valuable
substance for pharmacological use.
B0014
Presentation 10
(18:35~18:50)
Modulation of Cellular Properties of Fibroblasts Cultured in High-Glucose
Environments with Low-Intensity Pulsed Ultrasound
Da-Ming Huang and Shyh-Hau Wang
National Cheng Kung University, Taiwan
Abstract—Depending on such operational modes as the frequency,
exposure duration, and duty cycle, previous studies have shown that the
insonification of low-intensity pulsed ultrasound (LIPUS) may be
beneficial to the enhancement of wound healing in the skin tissue and to
the cellular properties. In the present study, effect of LIPUS was
investigated on the fibroblasts cultured in high-glucose (HG) environments
for mimicking to that of diabetic patients. Experiments were arranged to
allow LIPUS insonification into the murine fibroblasts, which were
cultured in various medium with the glucose concentrations ranging from
5.5 to 33 mM. The LIPUS consists of 1 MHz frequency, 50 mW/cm2
intensity, 20% duty cycle within 1 ms pulse repetition period, and 10
minutes exposure duration. The cellular properties, including viability and
stiffness of cell membrane, were respectively measured by XTT assay and
nano-indenter. Results show that the growth and stiffness from the
fibroblasts cultured in HG medium tended to decrease. Remarkably, both
the growth and stiffness of cell membrane of the fibroblasts cultured in HG
environments are improved after the LIPUS insonification. It suggests the
modulation of cellular properties and the improvement of stiffness of cell
membrane are owing to mainly the micro-vibration of LIPUS
insonification.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Session 9
Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session.
Afternoon, January 19, 2018 (Friday)
Time: 16:20~18:50
Venue: Room C6 of Global Front (17th Floor)
Topic: “Drug Development and Dynamics Analysis”
Session Chair: Assoc. Prof. Jayantha Gunaratne
B0058
Presentation 1
(16:20~16:35)
Degradation Kinetics of Cyanide and Uric acid in Bamboo Shoot during
Boiling Process
Sawanit Aichayawanich, Chanthima Phungamngoen, Jittimon Wongsa
and Thanya Parametthanuwat
King Mongkut’s University of Technology North Bangkok, Thailand
Abstract—The effects of boiling temperatures (60, 70, 80, 90, and 100oC)
and shape (whole shoots, slices, and rectangular rods) on the degradation
of cyanide and uric acid in bamboo shoots during the boiling process were
investigated. Boiling temperature was found to enhance cyanide and uric
acid degradation. While, the shape of the bamboo shoot effect on the
degradation of cyanide and uric acid on the whole shoot is lower than that
of sliced and rectangular shaped shoots, respectively. Kinetic modeling of
cyanide and uric acid degradation indicated that the degradation of cyanide
and uric acid could be defined using first-order and zero-order models,
respectively. Moreover, the Arrhenius equation can explain the effect of
temperatures on kinetic rate constant of cyanide and uric acid degradation.
The consumers accept the sensory properties of boiled bamboo shoots after
boiling for 18 min in all boiling temperature and bamboo shoot shape.
B0067
Presentation 2
(16:35~16:50)
Preparation and Characterization of Ca-alginate-chitosan Capsules for
Controlled Release of Imidacloprid as Larvaecide Delivery System
Lim Gim Pao and Ahmad Muhammad Syarhabil
School of Bioprocess Engineering, Universiti Malaysia Perlis, Malaysia
Abstract—Ca-alginate-chitosan based capsules were prepared and then
loaded with imidacloprid larvaecide for its controlled release against Aedes
aegypti larvae. The Ca-alginate-chitosan capsules containing imidacloprid
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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were characterized using FTIR and SEM. Imidacloprid encapsulation was
proven through HPLC analysis. Imidacloprid loading and encapsulation
efficiency were 5.9 % and 34 %. The in vitro release test showed that the
usage of Ca-alginate-chitosan capsules positively modified the release
profiles of the imidocloprid in water. The imidacloprid release kinetics fit
the Korsmeyer–Peppas model and follows the anomalous transport profile.
The bioassay results showed that Ca-alginate-chitosan capsule loaded with
imidacloprid is effective against Aedes aegypti larvae at lower doses than
that required by the technical grade imidacloprid for the control of Aedes
aegypti larvae.
B0075
Presentation 3
(16:50~17:05)
Mining Patterns of Drug-Disease Association from Biomedical Texts
Wen-Juan Hou, Bo-Syun Lee and Hung-Chi Chen
National Taiwan Normal University, Taiwan
Abstract—Drug repurposing aims to identify new indications for approved
drugs, and it can promisingly reduce time and drug development costs. The
goal of the paper, drug-disease relation extraction automatically from
biomedical texts, is fundamental to the study of drug repurposing since lots
of clinical case studies published in an unstructured textual form. To
analyze the number of verbs and nouns pertinent to diseases and
medications in the training data, two models with different drug-disease
orders are established, and some rules are proposed at this phase. The first
model is for the sentences with the order that the disease name precedes the
drug name. The second model is for the reverse order to the first model.
These verbs and nouns are then classified into categories of “pure
association,” “pure no association” and “neutrals.” Among them, some
neutrals are further verified by the Chi-square test method. As a result, the
associations between diseases and medications are identified, which are
called patterns later. Finally, the patterns are used in the test data to extract
the disease and drug pairs. The best experimental results show the
precision value of 100%, recall value of 89.0%, and F-score value of
94.2%.
B0083
Presentation 4
(17:05~17:20)
Single Administration of Resveratrol-Loaded Nanoparticles via Carotid
Artery Improves Neuronal Damage and Promotes Neuronal Regeneration
in Transient Middle Cerebral Artery Occlusion Rats
Huae Xu, Jie Zhong, Xiaolin Li and Xiaowei Lu
The First Affiliated Hospital with Nanjing Medical University, China
Abstract—Endovascular thrombectomy (EVT) retract clots and open
occluded arteries rapidly usually leads to ischemia/reperfusion (I/R) injury
which discounts the beneficial effect. Resveratrol (RES) was reported to
attenuate oxidative stress, inflammation and apoptosis related to I/R injury.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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However, its application was restricted by poor water solubility, short
half-life in vivo and low concentration in brain. We encapsulated RES in
biodegradable Poly(N-vinylpyrrolidone)-b-poly (ε-caprolactone)
nanoparticles (RES-NPs) and tested their efficacy in a rat Intraluminal
filament transient middle cerebral artery occlusion (tMCAO) model. A
carotid artery route of administration was applied to maximize drug
localization in the brain. Localized brain delivery of RES-NPs reduced
infarct volume, maintained BBB integrity and relieved edema through
neutralized ROS, atteneuated apoptosis. Neither NPs mixed with RES-Sol
nor RES-Sol alone was effective to protect brain. Survived rats treated with
RES-NPs demonstrated neurogenesis and regained vital neurological
functions.
B0087
Presentation 5
(17:20~17:35)
Superior Therapeutic Efficacy of Self-Assemble Paclitaxel Nano-Filaments
through Enhanced Induction of Autophagy in Gastric Cancer
Huae Xu, Wenling Zhang, Na Yu, Xiaolin Li and Weihao Sun
The First Affiliated Hospital with Nanjing Medical University, China
Abstract—The side effect of Paclitaxel (Ptx) has limited its clinical
application because of poor solubility. In the current study, we designed
novel self-assembled paclitaxel nano-filaments with extremely high drug
loading efficiency by conjugating Ptx and succinic acid (SA). The
conjugate Ptx-SA could self-assemble into nano-filaments with excellent
water solubility. Cytotoxicity test showed the superior effect of Ptx-SA
against gastric cancer cell lines SCG7901 and BGC823. Ptx-SA more
efficiently induced the apoptosis and autophagy-induced cell death than the
equivalent dose of free Ptx. More autophagosomes with increasing
autophagic flux was elicited by Ptx-SA in gastric cancer cells than free Ptx.
Ptx-SA induced the expression of LC3, a marker of autophagy, more
effectively than free Ptx. Moreover, Ptx-SA nano-filaments could
significantly enhance the in vivo anti-cancer effect of Ptx. Therefore, the
self-assemble nano-filaments of Ptx-SA is a potential way to strengthen the
therapeutic efficacy of Ptx in curing gastric cancer.
B0096
Presentation 6
(17:35~17:50)
Studies and Biological Significances of Medical Plant Microecology
Guo Xiaoheng and Yan Zhuyun
Chengdu University, China
Abstract—The relationship between plants and microbes began in
mycorrhizal, which involves in plant physiological and biochemical
metabolism and gene regulation. Due to the improvement of plant
nutrition, stress resistance, the safety of ecological environment,
maintaining and enhancing the sustainable productivity of ecosystems, it
has been widely concerned from abroad; Meanwhile, it was found that
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endophytic bacteria involved in plant physiological and biochemical
metabolism and co-metabolism, with the growth promotion and biological
control. Rhizosphere beneficial microbes and roots form a mutualistic
relationship, which can improve plant nutrition, growth, cological and
environmental safety. Beneficial phyllospheric microorganism participates
in plant carbon and nitrogen metabolism, enhancing disease resistance,
insects, etc. This paper summarizes some common and specific problems
of endophytes, mycorrhizal fungi and rhizosphere microorganisms, and
points out the development trend of research. This paper explores the scope
of microbial research of medicinal plants, and indicates that the life history
of plants accompanies with microbial nutrient cycling. It demonstrates that
the microecosystem of medicinal plants consists of microorganisms
(rhizospheric microorganisms, endophytes and phyllosphere microbes) and
their host-related microenvironment (tissue, cell, metabolite, secreta), that
is, a single medicinal plant is a Microecology. The article points out that
"herbs are the product of medicinal plant microecosystem, the quality of
medicine depends on the function of plant microecosystem", and points out
the focus of plant microecological research. The study of medicinal plant
microecology has unveiled plant microecological relationships and a new
way of microbial resources, which will have a wider impact in a number of
areas. The real significance of microbial research in medicinal plants lies in
the use of microbes and host plants to interact with each other to produce
safe and effective medicinal herbs to ensure the quality of traditional
Chinese medicine and realize the sustainable production and utilization of
Chinese herbal medicines.
B0027
Presentation 7
(17:50~18:05)
Study of Recyclability of EMIM-Ac in Rice Straw Pretreatment
Ratsarin Akkharasinphonrat, Kraipat Cheenkachorn, Surapun
Tepaamorndech, Atthasit Tawai and Malinee Sriariyanun
King Mongkut’s University of Technology, North Bangkok, Thailand
Abstract—Pretreatment is a vital process for the conversion of
lignocellulose biomass into energy. The pretreatment process is known to
enhance solubilization and dissociation of the complex lignocellulose
structure. This study focused on efficacy of ionic liquid pretreatment, using
1-ethyl-3-methylimidazolium acetate (EMIM-Ac), of rice straw for sugar
production in the comparison with acid and alkaline pretreatment. The
results showed that EMIM-Ac pretreatment resulted in dramatic changes in
chemical structures and microscopic morphology of rice straw. These
observations were associated with the high levels of sugar production.
Evaluation of EMIM-Ac recyclability demonstrated that absolute methanol
was an anti-solvent to yield the higher sugar levels than water and acetone.
In summary, these findings show that EMIM-Ac ionic liquid is the efficient
method for rice straw pretreatment due to high sugar yields and
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recyclability.
B0055
Presentation 8
(18:05~18:20)
A Potential Therapeutic Rutaecarpine Derivatives for Inflammation-
Induced lung Dysfunction
Chi-Ming Lee, Chiao-Han Yen and Chun-Mao Lin
Taipei Medical University, Taipei, Taiwan
Abstract—Lung fibrosis is a pathological state of excessive disposition of
extracellular matrix in an organ or tissue undergoing series process of
coagulation, inflammation, myofibroblast activation and continuous
reparative or reactive process. Rutaecarpine is the main effective
component of Evodiarutaecarpa which is the wide-used traditional Chinese
medicine. It presents vasodilation、anticoagulation and anti-inflammation
activity. However, the further application is limited by its cytotoxicity.
Thus, we had designed and synthesized a structural modifications of
rutaecarpine (RUT), 10-Fluoro-2-methoxy-rutaecarpine (F-RUT), showing
well anti-inflammation, anti-invasion and anti-migration activity with very
low cytotoxicity in cells. We also observed a raised level of Transient
receptor potential vanilloid type1 (TRPV1) and eNOS in F-RUT-treated
cell. These results suggest that RUT derivatives’ potential ability in
preventing vasorelaxation/hypertension. Particularly, the F-RUT
significantly reduced OVA/alum-induced lung inflammation and the
thickness of the alveolar wall in B6 mice, and also reduced the ROS in
LPS-stimulated zebrafish. The results reveal that F-RUT might be able to
prevent lung fibrosis. Based on the limited options for fibrosis treatments,
this novel compound provides a new insight to be a pharmaceutical
strategy of fibrosis mediated by inflammatory reaction, such as asthma.
B0071
Presentation 9
(18:20~18:35)
Development of Vi Polysaccharide Purification Method for Typhoid
Conjugate Vaccine Material: Effect of Ethanol Precipitation Variation to
Impurities and Vi Polysaccharide
Dea Marsendah and Wardono Niloperbowo
PT Biofarma, Indonesia
Abstract—One way to overcome typhoid fever is by immunization using
Vi polysaccharide vaccines. Purification method of Vi polysaccharide has
been developed but effectiveness not meet satisfactory since high
impurities still present. For that reason, research on purification method of
Vi polysaccharide was developed with focuses on the effect of ethanol
precipitation variation to impurities and Vi polysaccharide. Different
concentration of ethanol solutions (20%, 30%, 40% and 50%) were used to
remove impurities. Based on the measurement of endotoxin value and
nucleic acid from samples, precipitate washed with ethanol 30% gave
endotoxin value of 60% fraction at 261.7 EU/mL and nucleic acid at 0,243
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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mg/mL and it is the lowest among others. In this research also conducted 3
times repeated washing treatment with 20% and 30% ethanol solutions.
Precipitate washed 3 times with 20% ethanol gave endotoxin value of 60%
fraction at 139.3 EU/mL, while washing with ethanol 30% gave endotoxin
value at 102.5 EU/mL. Based on results, it can be concluded that addition
of 30% ethanol solution and repeated three times precipitation process
using 20% and 30% solutions can increase the removal of impurities
without reducing the gain of Vi polysaccharide.
B0079
Presentation 10
(18:35~18:50)
Continuous Quantitative Measurement of Lactic-Acid released from an in
vitro Mouse Brain Slice using Electrochemical Biosensor
Muhammad Izzat Firdaus bin Zailuddin, Shoto Kamada, Yoshiki Tojyo,
Ichiro Takashima, Hiroyuki Kudo and Riichi Kajiwara
Meiji University, Japan
Abstract—Although researches for lactic acid as an alternative energy
substrate have increased, its role during many neuronal activities states
remains unclear. The purpose of this research is to design the experimental
system for monitoring the lactic acid slightly released from a living brain
slice (350µm thickness). We combined the interface chamber for
electrophysiology with the microfluidic device where lactate sensor was
implemented. Lactate sensor has an electrode modified with the enzyme
Lactate Oxidase that has high selectivity and sensitivity towards lactate. By
using this system, we successfully measured the lactate signal (1.8 nA)
from the brain tissue under the perfusion of aCSF. Moreover, the signal
was dramatically increased to 7.7 nA when we decreased the glucose level
of the aCSF (10mM to 5mM). These results suggest that the brain slice
normally released a certain amount of lactic acid, and neuro-glial
interaction system in the brain might be triggered by the decrease of the
glucose level of the extracellular space. Since our system is capable for
simultaneous recordings of lactate signal and electrical activity of neurons
following electrical and/or chemical stimuli, it would be useful to
investigate the relationship between metabolism and neuronal activities
under various conditions.
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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Session 10
Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session.
Afternoon, January 19, 2018 (Friday)
Time: 16:20~18:50
Venue: Conference Room 1 of University Hall (3rd Floor )
Topic: “Environmental Quality Assessment and Management”
Session Chair: Prof. Zeng-Yei Hseu
T0010
Presentation 1
(16:20~16:35)
Double Environmental Management: Cationic Dye Removal on Sorbents
Obtained from Poly(Methyl Methacrylate) Waste
Dominik Zdybał, Małgorzata Skorupa, and Andrzej Milewski
Silesian University of Technology, Poland
Abstract—A new synthesis procedure for macroporous hydrogel was
developed. A possibility has been proven to obtain crosslinked, highly
water-swellable polymer matrix directly from non-crosslinked,
hydrophobic poly (methyl methacrylate) by a simultaneous
hydrolysis-crosslinking process in DMSO-diglyme solvent system. Waste
samples of PMMA, provided by automobile industry, have been harnessed
to obtain crosslinked poly (methacrylic acid)-co-poly (methyl
methacrylate) macroporous sorbents. Pure atactic PMMA was also used
to obtain a series of sorbents in order to optimize the synthesis conditions.
Rhodamine B was used as a model cationic dye for kinetics studies,
isotherm evaluation and to determine pH-dependent ability to remove the
contamination. Nano/micro-Fe3O4 fillings were added to amend sorbents
reuse by magnetic sedimentation process. Regeneration of sorbents has
been performed using a green solvent system. In summary, a new strategy
has been proposed to manage plastic waste, remove contamination from
effluents and reuse the dye.
T3001
Presentation 2
(16:35~16:50)
Magnetic Amphiprotic Catalysts and Separators Coping with
Environmental Issues
Byung Kwon Kaang and Won San Choi
Hanbat National University, Korea
Abstract—Amphiprotic catalysts have recently gained increased interest
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regarding applications in oil/water separation and pollutant purification.
Many industries emit oil/water mixtures containing pollutants as
wastewater. The amphiprotic catalysts reported thus far have used
separated processes for oil/water separation and pollutant purification,
which is not suitable for industrial applications requiring in-situ and
continuous processes for oil/water separation and pollutant purification.
Amphiprotic catalysts that can freely move between two phases, such as oil
and water, to perform functions in each phase, such as the decomposition
of aqueous and non-aqueous pollutants, have not been reported. Here, we
report a novel approach-based magnetic amphiprotic catalyst (MAC) that
can freely move between oil and water to perform functions in each phase,
enabling the in-situ and continuous processes for oil/water separation and
the decomposition of aqueous and non-aqueous pollutants in both phases.
T3002
Presentation 3
(16:50~17:05)
Surface Design of Separators for Oil/Water Separation with High
Separation Capacity and Mechanical Stability
Nara Han and Won San Choi
Hanbat National University, Korea
Abstract—A convection heat treatment that can replace existing chemical
oxidation methods was developed for the preparation of hierarchically
oxidized Cu meshes with various surface morphologies, representing a
very simple and green route that does not involve toxic chemicals. Three
types of Cu meshes [bumpy-like (BL) and short and long needle-like (NL)
structures] exhibited similar separation efficiencies of 95−99% over 20
separation cycles, as indicated by their similar water contact angles
(WCAs; 147−150°). However, these Cu meshes exhibited different flux
behaviors. Excessively rough and excessively smooth surfaces of the Cu
mesh resulted in increased resistance to flow and to a decrease of the
penetration of oil. A surface with intermediate smoothness, such as the
BL-Cu mesh, was necessary for high flux over a broad range of oil
viscosities. Furthermore, a less rough surface was more suitable for the
separation of highly viscous oil. Computational fluid dynamics (CFD)
simulations were carried out to support our experimental results. The
BL-Cu meshes also showed outstanding mechanical stability because of
their low resistance to the flow of fluids.
T3003
Presentation 4
(17:05~17:20)
Self-floating and Submerging Sponge Adsorbents for Environmental
Remediation
Yoseph Lee and Won San Choi
Hanbat National University, Korea
Abstract—Water pollution by heavy metals and organic dyes has become a
serious environment issue. Diverse technologies have been reported to
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eliminate these pollutants. Among these methods, nanoadsorbents are of
particular interest in wastewater treatment because of their high
surface-to-volume ratio, which leads to a higher uptake capacity. However,
most of the reported nanoadsorbents are flow type, which are very
vulnerable to leakage in the process of separation because they are
dispersed or precipitated. It has been recently reported that the unintended
leakage of nanomaterials into the environment can cause a significant
threat to the environment and public health. Here, we report a janus sponge
composite that can be partially floated/submerged on/in the water to
perform efficient adsorption of pollutants without leakage of adsorbents.
The janus sponge composites not only prevent adsorbents leakage but also
enable to remove heavy metal ion and carry out oil-water separation at the
same time.
T3004
Presentation 5
(17:20~17:35)
Accounting for the Effect of Temperature in Clarifying the Response of
Foliar Nitrogen Isotope Ratios to Atmospheric Nitrogen Deposition
Guoan Wang and Chongjuan Chen
China Agricultural University, China
Abstract—Atmospheric nitrogen deposition affects nitrogen isotope
composition (δ15
N) in plants. However, both negative effect and positive
effect have been reported. The effects of climate on plant δ15
N have not
been corrected for in previous studies, this has impeded discovery of a true
effect of atmospheric N deposition on plant δ15
N. To obtain a more reliable
result, it is necessary to correct for the effects of climatic factors. Here, we
measured δ15
N and N contents of plants and soil in Baiwangshan and
Mount Dongling, north China. Atmospheric N deposition in Baiwangshan
was much higher than Mount Dongling. Generally, however, foliar N
contents showed no difference between the two regions and foliar δ15
N was
significantly lower in Baiwangshan than Mount Dongling. The corrected
foliar δ15
N after accounting for a predicted value assumed to vary with
temperature was obviously more negative in Baiwangshan than Mount
Dongling. These results indicated that atmospheric N deposition had a
negative effect on plant δ15
N. Additionally, this study also showed that the
corrected foliar δ15
N of bulk data set increased with altitude above 1300 m
in Mount Dongling, this provided an another evidence for the conclusion
that atmospheric N deposition could cause 15
N-depletion in plants.
T3007
Presentation 6
(17:35~17:50)
A Novel Sulfide Control Strategy Using Conductive Iron Oxide in
Anaerobic Co-Digestion of Macroalgal Ulva Biomass with Cheese Whey
Heejung Jung, Jaai Kim, and Changsoo Lee
Ulsan National Institute of Science and Technology (UNIST), Republic of
Korea
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Abstract— Anaerobic digestion of sulfur-rich feedstock entails a concern
with sulfate reduction which produces sulfide, toxic to methanogenesis.
Conventional strategies for sulfide removal via precipitation by using iron
species are expensive and inefficient. This study studied the potential of
new approach using magnetite, conductive iron oxide, for sulfide control in
the co-digestion of sulfur rich Ulva biomass with cheese whey. Compared
to the control reactor without magnetite addition (0.55–0.83% H2S in
biogas, v/v), the magnetite-added reactor showed significant decreases in
the H2S content down to <0.02% (v/v) with increase in magnetite dosage
(0–8 mM Fe). The Raman spectra of the magnetite-added reactor effluent
corresponded the peak profile of elemental sulfur, but not that of iron
sulfide, which means that sulfate from the Ulva degradation was likely
transformed to elemental sulfur in the presence of magnetite. This likely
suggests that the oxidation of sulfide, produced by sulfate reduction, to
elemental sulfur occurred in the reactor possibly by direct interspecies
electron transfer between electroactive bacteria via magnetite. Although
the detailed mechanism is unclear, this suggests an interesting potential for
in-situ sulfide control and sulfur recovery.
T0016
Presentation 7
(17:50~18:05)
Organizational Carbon Footprint of King Mongkut's University of
Technology Thonburi
Pisitpong Intarapong and Amnat Chidthaisong
King Mongkut’s University of Technology Thonburi (KMUTT), Thailand
Abstract— A comprehensive carbon footprint assessment of organization
has been analyzed from direct and indirect greenhouse gases (GHG) in all
activities. This work aims to evaluate the carbon footprint of an academic
institution in engineering department of King Mongkut's University of
Technology Thonburi (KMUTT), Ratchaburi campus as a residential
college in Thailand and to develop alternative opportunities for GHG
reduction. A multicriterial assessment from the following activities such as
transportation, electricity consumption, materials uses, solid and water
waste, construction, land use, and other energy usages was considered. The
results showed that total carbon footprint collected from the college in
2015 was 981.16 tCO2e/yr. The average carbon footprint per capita was
2.73 tCO2e/yr. The most GHG emissions of 533.3 tCO2e/yr were caused by
electricity consumption, accounting for 54.36 % of the overall GHG
emissions. Building, land use, and solid waste generated GHG emission of
17.48, 12.14, and 6.39 % of the overall GHG emissions, respectively. The
appropriate strategies of GHG reduction have been proposed in order to
reduce the average carbon footprint per capita less than 2.50 tCO2e/yr.
Furthermore, carbon neutrality should be investigated in the campus.
T4006 Changing Organic Matter Characteristics of Littoral Sediment by Solar
Cell-Combined Sediment Microbial Fuel Cell
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Presentation 8
(18:05~18:20)
Narong Touch, Hiroki Takata, Satoshi Yamaji, and Tadashi Hibino
Hiroshima University, Japan
Abstract—Metal ion or clay mineral-adsorbed organic matter present in
littoral sediment is known as hardly decomposed organic matter, which is
difficult to use as a natural resource. This study is aimed at changing the
organic matter characteristics of littoral sediment through the application of
solar cell-combined sediment microbial fuel cell (SC-SMFC). The
experimental results showed that the sediment pH decreases and the
concentration of metal ions in the sediment pore water increases after the
application of SC-SMFC. This suggests the dissociation of metal
complexes in the sediment. From the analysis results of organic matter
characteristics, variations in the ignition characteristics of the sediment and
the absorbance at wave number ranges of 3300–3800 and 800–1800 cm-1
were confirmed, indicating changes in organic matter characteristics of the
sediment. It can be concluded that SC-SMFC can separate organic matter
from metal complexes, leading to the transformation of organic matter in
sediment from valueless into a useful product.
T0028
Presentation 9
(18:20~18:35)
Developing Hydrodynamic and Sediment Transport Modelling on Lakes: A
Preliminary Study
Rian Mantasa Salve Prastica, Herr Soeryantono, and Dwinanti Rika
Marthanty
Universitas Indonesia, Indonesia
Abstract—Lakes in Universitas Indonesia (UI) have several problems to
overcome. Water quality of UI’s lakes degrades in the last decade. One of
the problems is high turbidity. The longer turbidity in lakes, the more
dangerous the lakes environment life would be. So, this research focuses
on sediments problem or physical assessment. The main research aims to
develop hydrodynamic and sediment transport modelling of Agathis and
Mahoni Lakes in Universitas Indonesia. The authors want to increase the
quality of water in UI’s lakes and make both Agathis and Mahoni Lakes as
the representative preliminary model. Research design or methodology is
needed to concept the research to achieve goals. Unfortunately, research
methodology has not been developed yet for this case. This paper goals to
construct methodology as a preliminary study of the main research. The
authors use extensive literature review to model novel methodology.
Hydrodynamic and sediment transport modelling could be modelled by
Resources Modelling Associates (RMA). Hydrodynamic phenomena in
fluid and sediment transport modelling have the suitable governing
equation concept with RMA’s governing equation. As a thinking result, the
main research would be conducted by RMA program modelling, field
sampling, and laboratory testing. Field sampling and laboratory could
figure the amount of sediment concentration in lakes as data input. The
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final simulation of this following research is making sensitivity and
scenario analysis to prove several hypotheses.
T0009
Presentation 10
(18:35~18:50)
Color Glasses during Morning Drive for Commuting: Effects on
Autonomic Functions, Alertness, and Nocturnal Sleep
Emi Yuda, Yutaka Yoshida, and Junichiro Hayano
Nagoya City University, Japan
Abstract—Studies of illumination suggest possibility of color lights for
adjusting our mind and body to suit desired activities. To examine if
similar effects can be obtained by modulating relative contents of light
color, biological effects of color-glass wearing during early-morning drive
for commuting were studied. Compared with clear glasses, blue glasses
increased low frequency (0.04-0.15 Hz) power and deceleration capacity
and decreased respiration frequency during driving, while green, orange,
and pink glasses made no significant difference from clear glasses. None of
the color glasses showed significant difference in the performance to
psychomotor vigilance test performed at the beginning and the end of work
at job place or in the autonomic functions during sleep of that night. An
increase in the content of blue-wavelength light by blue glasses during
morning drive may attenuate sympathetic function, but it has no significant
after-effect on daytime behavioral alertness or nighttime autonomic
functions.
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Poster Session January 18, 2018 (Thursday)
Time: 13:00 ~18:25
Venue: Lounge in Front of C5
B0004
Presentation 1
Sesamin Induces Cell Cycle Arrest and Apoptosis of Human Cervical
caRcinoma Cells Via Inhibition of PI3K/ERK and Upregulation of
p53/p21
Chun-Shiang Lin, Kuan-Te Li and Shao-Hsuan Kao
Chung Shan Medical University, Taiwan
Abstract—Cervical cancer is an important life-threaten malignancy to
women in Taiwan and the world. Recently, sesamin, a major component
in sesame seeds, has been widely explored with emphasis on antitumoral
activity against several cancer cells. Here, we aimed to explore whether
sesamin possesses antitumoral activity against human cervical cancer
cells. By using MTT assay, we found that sesamin (15 - 300 uM) lowered
cell viability of Hela and SiHa cell in a dose-dependent manner. Flow
cytometric analysis showed that sesamin increased sub-G1 ratio up to
44% (150 uM). Cell cycle regulators were determined by using
immunoblotting and the results showed that sesamin upregulated p53 and
the downstream p21, while downregulating cyclin A, cyclin B, and cdc2
in Hela cell. We further observed that sesamin inhibited survival
PI3K/ERK activation, which may attribute to upregulation of PTEN. In
addition, we also found that sesamin induced apoptotic cascades
including cleavage of caspase-9, caspase-3, and poly(ADP-ribose)
polymerase (PARP) in Hela cell, but lowered anti-apoptotic Bcl-2 level
and elevated pro-apoptotic Bax level. Collectively, these findings indicate
that sesamin can inhibit cell proliferation of human cervical cancer cells,
which may attribute to upregulation of p53 and p21, and the following
activation of apoptotic signaling and suppression of survival signaling,
contributing to G1 arrest and the further apoptosis. Accordingly, we
suggest that sesamin could be beneficial to cervical cancer treatment.
B0022
Presentation 2
The Influence of VR Color image for Color Psychotherapy
Geeyun Hong, Jiwon Lee, Cho-I Moon, Songee Kim and Onseok Lee
Soonchunhyang University, Republic of Korea
Abstract—Color therapy is a diagnostic method for treating psychological
2018 CBEES-BBS TOKYO, JAPAN CONFERENCE
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problems using unique vibration and frequency color. The color has
strong subjective psychology, it can give psychological stabilization
effect as a spring as a favorite color and stability become personally
personal. Also, to give various influences on emotion, we can obtain
information via brain wave to evaluate these emotions. In this research,
after viewing two kinds of VR color images of red and blue via a mobile
terminal, we used four emotional data measurable via brain waves and
grasped the user's emotional state. In addition, the stress questionnaire
was used to quantify the emotion degree, and comparative analysis was
made on how the color image affects the change of emotions with 3D
virtual reality.
B0023
Presentation 3
Segmentation for Bioluminescence Imaging of Small Animals
Cho-I Moon, Geeyun Hong, Jiwon Lee, Songee Kim and Onseok Lee
Soonchunhyang University, Republic of Korea
Abstract—Optical molecular imaging is less costly than other medical
imaging and is in the spotlight because of its ability to detect cancer in a
short time. However, it is not easy to automatically segment the cancer
because the resolution of the image is low. In this paper, we have
performed a study to automatically segment the region of interest in
optical molecular images using the level-based method; the
reaction-diffusion level set evolution (RDLSE) algorithm. RDLSE
showed the best performances by statistically comparing performance of
various methods with visually segmented ground truth. RDLSE is robust
to the segmentation of optical molecular images, as it compensates for the
disadvantages of other methods used in the experiments.
B0024
Presentation 4
A Study of the 3D Anatomical Structure Analysis of Bio-Sample by
AMIRA: Image Processing Application
Jiwon Lee, Cho-I Moon, Geeyun Hong, Eunyoung Kim and Onseok Lee
Soonchunhyang University, Republic of Korea
Abstract—In this study, we aim to obtain the fundamental data needed for
the objective analysis and bio-mimetics of leeches, using x-ray imaging
with radiation that allows 3D analysis of the detailed anatomic structures
of leeches. It was confirmed that through using radiation, detailed images
of leeches can be obtained, allowing 3D analysis of leeches’ anatomical
structures. Also, since the data obtained through the radiation allows you
to observe the micro-structure, it can serve as a good resource for
component analysis, as well as physiological and functional research.
More, it is hoped to contribute to further research in the areas of
bio-mimetics using leeches.
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B0030
Presentation 5
Protective Effects of Artocarpus Lakoocha Heartwood Extract in
UVB-Irradiated Human Keratinocytes, HaCaT Cells
Kittiya Malaniyom, Umalee Namdaung, Sunit Suksamrarn, Orapin
Wongsawatkul and Yamaratee Jaisin
Srinakharinwirot University, Thailand
Abstract—Excessive exposure to UVB radiation (220-320 nm) causes
oxidative damage to skin, resulting in sunburn, aging, and skin cancer.
Artocarpus lakoocha (A. lakoocha) heartwood extract is a rich source of
phenolic compounds conferring high antioxidant activity. The present
investigation was undertaken to determine the effects of A.lakoocha
heartwood extract in UVB- irradiated HaCaT cells. To obtain biologically
relevant information, A. lakoocha heartwood extract was subjected to
resazurin assay and antioxidant assays including DPPH scavenging assay
and griess assay. Results reveal that pretreatment the cells with
A.lakoocha heartwood extract significantly increased cell viability and
decreased nitric oxide levels in a concentration dependent manner.
A.lakoocha heartwood extract (0.25-40 g/ml) was not toxic to the cells.
Moreover, A.lakoocha heartwood extract showed a scavenging effect to
DPPH radicals. Taken together, we suggested that the protective effect of
A.lakoocha occur due mainly to antioxidant activity which provides it to
be a herbal medicine for preventing UVB-induced skin damage.
However, the further investigation through an intervening mechanism
needs to be evaluated.
B0037
Presentation 6
Flavonoids from the Roots of Scutellaria Baicalensis Possess Inhibitory
Activity against Proprotein Convertase Subtilisin/Kexin Type 9 mRNA
Expression
Young-Won Chin, Young Hee Choi, Byoung Hoon You1 and Young Mi
Kim
Dongguk University-Seoul, South Korea
Abstract—Nine flavonoids were isolated and identified from the roots of
Scutellaria baicalensis through a bioactivity-guided fractionation. This
method utilized a proprotein convertase subtilisin/kexin type 9 (PCSK9)
monitoring assay, which also identified nine known compounds from a
chloroform-soluble fraction. All structures were established by
interpreting spectroscopic data and comparing measured values from
those in the literature. All compounds were assessed for the ability to
inhibit PCSK9 mRNA expression; compounds 1
(3,7,2'-trihydroxy-5-methoxy-flavanone) and 4 (skullcapflavone II) were
found to be active. Furthermore, compound 1 was found to increase
low-density lipoprotein receptor protein expression.
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B0042
Presentation 7
Protective Effect of Limonene Against Corticosterone-Induced
Neurotoxicity
Somrudee Saiyudthong, Chantana Mekseepralard, Yamaratee Jaisin and
Punnee Nusuetrong
Srinakrarinwirot University, Thailand
Abstract—Limonene is a major aromatic compound in citrus oils which
have long been popular in aromatherapeutic practice due to reducing
physiological and psychological stress. However, its mechanism has not
been elucidated. Therefore, the aim of this study was to investigate the
protective effect of limonene on corticosterone-induced neurotoxicity in
PC12 cells. Methyl thiazolyl tetrazolium (MTT) assay, lactate
dehydrogenase (LDH) detection and flow cytometry were investigated to
confirm the neuroprotective effect of limonene against cell damage
caused by corticosterone. PC12 was treated with corticosterone 500 µM
and difference concentration of limonene (50, 100, 200, 300, 400, 500
and 600 µM) for 24 hours. Then, cells were determined the cell viability
using the MTT assay, LDH release from the cells due to corticosterone
-induced cytotoxicity and apotosis using flow cytometer assays,
respectively. Pre-incubating PC12 with 100 and 200 µM of limonene
significantly increased cell viability compared with the group treated with
corticosterone alone (p <0.01 and p <0.05, respectively). Limonene at the
concentration of 100 µM (p < 0.01) 200 µM and 300 µM (p < 0.05)
significantly reduced LDH releasing. Pre-treatment with limonene 100
and 200 µM caused higher PC12 number (p < 0.001) than those treated
with corticosterone alone. These findings indicated that limonene exerted
a neuroprotective activity against corticosterone-induced neuronal
damage. Further studies need to investigated the precise cellular and
molecular mechanisms underlying this neuroprotective activity of
limonene.
B0049
Presentation 8
Protective Effects of Beta-Mangostin against 6-OHDA-Induced
Caspase-3 Activation in SH-SY5Y Cells
Yamaratee Jaisin, Piyanee Rattanachamnong, Kittiya Malaniyom and
Sunit Suksamrarn
Srinakharinwirot University, Thailand
Abstract—Oxidative stress plays a pivotal role in Parkinson’s disease
(PD). 6-Hydroxydropamine (6-OHDA) has been widely used as a
neurotoxin to generate both in in vivo as well as in in vitro models of PD.
-Mangostin is a xanthone isolated from the pericarps of mangosteen
(Garcinia mangostana Linn), a “queen of fruit” in its native Thailand.
There is a scare study of -mangostin. We thus investigate its effect
against 6-OHDA-induced toxicity in human neuroblastoma SH-SY5Y
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cells. Resazurin assay, ROS assay, and Caspase-3 fluorometric assay
were used to evaluate the protective effect of -mangostin on oxidative
stress-triggered neurotoxicity. It was found that after pretreatment with
-mangostin at the concentrations of 1 and 2.5 M before exposure to
6-OHDA (50 M) for 1 h; the cell viability has a significant increase and
the amount of intracellular free radicals as well as the activity of
caspase-3 were decreased. Taken together, the results of this study
indicate that -mangostin protects SH-SY5Y cells from toxic effects of
6-OHDA via its antioxidative effect. We suggest that it may have a
therapeutic potential in preventing oxidative stress which would be a
causative factor for inducing pathogenesis of PD.
B0053
Presentation 9
Wood-Plastic Biocomposites Prepared from Recycled High Density
Polyethylene Bottles and Wood Flour: A Comparative Study of Virgin
High Density Polyethylene
Nawadon Petchwattana
Srinakharinwirot University, Thailand
Abstract—The effect of wood flour (WF) contents on the mechanical,
physical, thermal and morphological properties of recycled high density
polyethylene (rHDPE) and virgin HDPE (vHDPE) and WF composites
were investigated in the current research. Both the rHDPE and vHDPE
composites were less stiff with the addition of WF. Their flexural and the
tensile strengths decreased with the WF contents. Microscopic
observations revealed some WF pulled-out particles and numerous
interfacial cavities within composites. Total water absorption at 90th day
increased with increasing WF content. In comparison, the composites
with rHDPE seemed to exhibited stiffer manner due to some in organic
additive added in the HDPE bottles. All the experimental results indicated
that recycled HDPE is possibly used as WPC matrix instead of using
virgin HDPE.
B0057
Presentation 10
Feasibility Application of Biodegradable Active Films Produced by
Poly(lactic acid) Incorporating with Limonene as An Antimicrobial
Packaging for Fresh Beef Meat
Sarinya Sangkasanya, Phisut Naknaen and Nawadon Petchwattana
Srinakharinwirot University, Thailand
Abstract—A novel composite-based packaging was prepared by blending
poly(lactic acid) (PLA) with various concentrations (0, 4, 6 and 8%, w/w)
of limonene and was applied to prolong the shelf life of fresh beef meat
during cold storage. The barrier properties of the prepared films and the
microbiological qualities of packed ground beef were evaluated. Both
water vapor permeability (WVP) and oxygen transmission rate (OTR)
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were reduced as the contents of limonene increased. To apply the PLA
bags containing limonene to food packaging, the beef meat was packed
with the films and stored at 4oC for 12 day. The total viable count (TVB),
total coliform, Pseudomonas spp. and Staphylococcus aureus were
monitored. According to microbiological standard, the shelf life of the
control sample and the sample packed with PLA-limonene (4%) was
approximately 6 days while the samples packed with PLA-limonene at
the concentration of 6 and 8% were assured for at least 9 and 12 days of
storage, respectively. These results indicate that the PLA containing
limonene can be utilized as an antimicrobial packaging for the beef meat.
B0100
Presentation 11
Simultaneous Synthesis of Gold Nanoparticles in Dual Chamber
Microbial Fuel Cell and Their Application to Enhance Bio-Energy
Production
Md Rezaul Karim, Thi Hiep Han and Moo Hwan Cho
Yeungnam University, Republic of Korea
Abstract—In the present work, the development of Microbial fuel cells
(MFCs) was investigated by increasing performance, reducing cost and
novel application of this system. Gold nanoparticles (AuNPs) showed
highest stability among other nanoparticles and can be used as an
excellent material to modify electrode surface. In our previous work,
inexpensive three-dimensional (3D) N-doped open-porous carbon foam
anode was prepared by calcination of melamine sponge in nitrogen
environment. In this study, simultaneous bio-synthesis and decoration of
as-prepared N-doped carbon foam by AuNPs using Shewanella
oneidensis MR1 were performed. As a result, AuNPs were successfully
synthesized by S.oneidensis MR1 in MFC anode for the first time.
Moreover, the AuNPs decorated carbon foam anode produced a power
density of 46 W/m3, which was 53.3% higher than that of the bare carbon
foam anode. Cyclic voltammetry (CV) of bare and AuNPs decorated
carbon foam supported the statement that the AuNPs decorated showed
higher capacitance currents. The highly conductive AuNPs increased
surface areas, electron transfer rate along with decreased interfacial
electron transfer resistance to step-up the MFCs performance. In addition
bio-synthesize of gold nanoparticle in MFCs anode chamber is one step,
environment friendly and also an economical process.
B0101
Presentation 12
Mussel-inspired Bio-adhesive Polymer Based Drug Delivery System and
its Biomedical Application
Seung-Hyun Kim, Mira Cho and Jae-Hyung Jang
Yonsei University, Republic of Korea
Abstract—Mussels show strong adhesion properties in the dynamic sea.
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Several previous studies demonstrated that the key element for their
adhesiveness in wet condition is the self-polymerization of catechol
functional groups in the mussel proteins and the catechol group
conjugated polymers possess the same adhesiveness. In this study, the
catechol-polymer based biocompatible and bio-adhesive drug delivery
system is used for the localized and sustained drug delivery combining
with the adeno-associated viral vector (AAV) and as a result, fast drug
adsorption and localized release of AAV were observed. Furthermore, the
physical properties (e.g. pore-size, release profiles) of this drug carrier
could be regulated for efficient therapeutic conditions. We expect that this
catechol based bio-adhesive drug delivery system could be adapted to
various drugs not only therapeutic AAV based drugs but also small
chemical and large therapeutic cells. As a result, this bio-adhesive based
drug delivery system is expected to cover a variety of biomedical
applications.
B0103
Presentation 13
Turritopsis spp. Proteins Inducing Morphological Changes of Human
Cancer Cells
Youhei Kitada, Yuki Ichii, Tatsuki Kikegawa, Kenji Etchuya, Shin
Kubota and Yuri Mukai
Meiji university, Japan
Abstract—Premature creatures including butterfly, frog and medusa,
grow up to mature individuals by repeating morphological changes. The
morphological changes are caused by cell apoptosis and differentiation
inducing factors. Detection and identification of these factors are thought
to be useful as the application of cancer therapy research and regenerative
medicine. Turritopsis spp. is one kind of medusae known as “the
immortal jellyfish” and changes itself into dumpling and polyp immatures
without dying. This phenomenon which is caused when the mature
individual approaches the time of death, is just like “rejuvenation”. In this
study, the proteins of Turritopsis spp. were analyzed to find the novel
factors for the medical treatment of human diseases.
The body fluid of Turritopsis spp. was added to the cultured human
cancer cells to confirm the interaction between the proteins of Turritopsis
spp. and human cells. As a result, almost all of the cancer cells were
changed from normal diamond shape into spherical shape. The cell
viability after morphological change was confirmed by cell staining.
Moreover, the comprehensive detection of the proteins of Turritopsis spp.
which have ability of inducing morphological changes of human cells
was conducted using "2-D Cell Blot Method" developed in our
laboratory.
B3007 Natural Dyeing of Silk Dyed with Curcuma longa Extracts Dyed in
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Presentation 14
Yellow among Obangsaek
Jin Soun Jung
Semyung University, South Korea
Abstract— As the level of human living has improved recently and
interest in health has increased, substantial interest in well-being has
increased. Natural dyeing is a nature-friendly dyeing which is dyed with
natural materials. Therefore, it gives a lot of benefits to humans such as
the treatment of skin diseases and the insecticidal effect. Natural dyes can
be obtained in various colors depending on the dyeing method and use of
the mordant, even if the dye is taken from the same plant. In addition, the
various colors thus obtained are low in saturation, resulting in a subdued
color. Thus, the colors of fabrics obtained from natural dyeing are in
harmony with each other without special color harmony and make people
feel natural and comfortable. As each country has different cultures, color
images vary from country to country. Color image is a measure of a
society's culture and citizenship. Obangsaek is a unique color of Korean
nation. It is based on five colors of blue, red, white, black and yellow. In
this study, the natural material used for natural dyeing was Curcuma
longa which is dyed yellow, one of the five colors of Obangsaek. The
surface color, fastness, UV protection activity, deodorization, and
antimicrobial activity of the fabrics dyed with Curcuma longa extracts
was measured. The results are as follows. The color of the silk fabric
dyed with Curcuma longa extracts was 0.6Y and yellowish. Also its CIE
a* value was 7.59 and b* value was 51.34, which was slightly reddish
yellow. The V value of Munsell showing the lightness was 7.7 and the
corresponding CIE L* value was 77.92, indicating a relatively bright
color. The saturation C which indicates the degree of clearness of the
color was 7.9, indicating a medium saturation. Its dry cleaning fastness
was excellent in grades 4 to 5, including discoloration. Its perspiration
fastness was high in grade 4, both in acidity and alkalinity. In the case of
rubbing fastness, both the dry state and the wet state were excellent in 4
grade. The light fastness was 1 grade and lower than other fastness. The
sun protection factor was 5.4, indicating no UV protection effect. And It
showed 99.9% of antimicrobial activity on staphylococcus aureus ATCC
653B and Klebsiella pneumoniae ATCC 4352. Therefore, it was found
that it has a high antimicrobial activity on the two bacteria.
B3008
Presentation 15
Influence of Potato Virus YO in Autophagy of Nicotiana Benthamiana
Hyeonsook Cheong, Jaeyoung Park and Wonjin Kim
Chosun University, Korea
Abstract—Autophagy plays a critical role in the innate immune response
of plants to pathogen infection. In the present study, we examined
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autophagy induced by PVYO infection in Nicotiana benthamiana. ELISA
revealed that the number of virus particles in the plant peaked at 2 weeks
gradually decreased. Also, PVYO increased in the 3rd and 4th leaves and
decreased in the 5th leaf. Moreover, PVYO infection caused changes in
the number, size, and shape of chloroplasts, whereas the number of
plastogranules increased. Furthermore, double-membrane
autophagosome-like vacuoles, including electron-dense materials,
laminated structures, and cellular organelles, were found. The induction
of autophagy after the PVYO infection of leaves was further confirmed by
the expression of lipidated microtubule-associated LC3-II, an autophagy
marker and p62, an autophagy adaptor protein. The LC3-II levels
increased daily over the 4-week period. Although virus inoculation was
performed on the basal leaves, LC3-II was expressed throughout the
leaves and the expression was higher in leaves distal to the inoculated
leaf. PVYO infection caused the activation of stress-activated protein
kinases/c-Jun N-terminal kinases. Therefore, PVYO infection-induced
autophagy was positively correlated with the virus content, suggesting
that autophagy induction following PVYO infection is involved in the
antipathogen response of the host.
B3009
Presentation 16
Regulation of Adipocyte Gene Expression and Differentiation by
Funtional Plant P. densiflora
Woong Kim, Jaeyoung Park, Beomgi Lee, Cheolwoo Park, Wonjin Kim
and Hyeonsook Cheong
Chosun University, Korea
Abstract—Obesity is a common disease in worldwide and considered a
significant risk factor causing various complications. Pinus densiflora
sieb. et zucc contains several natural compounds that have various
biological activities. However, the health beneficial effects of these
compounds have rarely been reported. In this study, to evaluate the
inhibitory effect of pine needle extract powder(PNEP) on adipocyte
differentiation in 3T3-L1 preadipocytes. The level of adipogenesis in the
3T3-L1 cells was measured by Oil Red O staining. As a result of Oil Red
O staining, PNEP significantly inhibited adipocyte differentiation by
more than 55% in 3T3-L1 preadipocytes in a dose-dependent manner.
Furthermore, We confirmed PNEP inhibited adipocyte differentiation by
suppressing the expression of the adipogenic transcription factors Fatty
acid binding protein(aP2) and Sterol regulatory element binding
transcription factor 1(SREBP1c) using real-time PCR analysis. These
results shows that PNEP inhibits adipogenesis by suppressing the
expression of adipogenic transcription factors.
B3010 Bioactivity of UVB Induced Skin Inflammation Factor in HaCaT Cells by
Needle Extract of P. densiflora
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Presentation 17
Cheolwoo Park, Woong kim, Jaeyoung Park, Beomgi Lee, Wonjin Kim
and Hyeonsook Cheong
Chosun University, Korea
Abstract—Ultraviolet B (UVB) irradiation is known to induce
inflammation, oxygen stress, skin cancer and photo-aging. UVB
irradiation generates intracellular reactive oxygen species (ROS), and
induced matrix metalloproteinase-1 (MMP-1) and pro-colleagenase.
Pinus densiflora Sieb. et Zucc. (red pine) has various biological activities
such as antimicrobial and antioxidant. However, the skin
anti-inflammation effects of red pine have rarely been reported. In this
study, we partitioned n-Hexane, EtOAc n-BuOH and H2O layer from
methanolic extract of red pine needles and tested anti-inflammation
activity on . As a result, Inhibition of TNF-α induced IL-1β expression by
n-BuOH layer than other layers. We check up on the effects of n-BuOH
layer on inflammation, photo-aging and investigated its mechanism of
action in human keratinocytes HaCaT cells. HaCaT Cells were then
exposed to UVB light (15 mJ/㎠). DCF-DA assay revealed that n-BuOH
layer (25-100 ug/mL) inhibited intracellular ROS. Furthermore, MMP-1
level, which has been known to be induced by ROS, was significantly
reduced by n-BuOH layer (25-100 ug/mL) on Real-Time PCR. Moreover,
UVB induced pro-inflammatory cytokine factors(IL-6, IL-8, TNF-a and
COX-2) were significantly suppressed by n-BuOH layer (25-100 ug/mL)
in a dose-dependent manner. Chromatography purification and
fractionation of n-BuOH layer were conducted to prove suppression
UVB-induced ROS, MMP-1 and inflammation factor level.
T0007
Presentation 18
Alcohol Drinking Strengthens the Association between Ambient Air
Pollution and Liver Enzymes
Hyun-Jin Kim, Jin-young Min, Yong-Seok Seo, and Kyoung-bok Min
Seoul National University, Korea
Abstract—Some studies have found a link between air pollutant exposure
and liver enzyme levels in restricted areas or elderly people, but the
available data cannot generalize. This nationwide study of South Korea
adults investigated the association between air pollution, liver enzyme
levels, and alcohol consumption. The annual average concentrations of
the air pollutants including particulate matter with an aerodynamic
diameter £10 mm (PM10), nitrogen dioxide (NO2), sulfur dioxide (SO2),
and carbon monoxide (CO) were calculated. Liver enzymes included
alanine aminotransferase (ALT) and aspartate aminotransferase (AST).
The PM10, NO2, SO2, and CO were found to be significantly associated
with elevated ALT and AST levels, with increase ranged from
0.0073IU/L(95% CI =0.0042, 0.0104) to 0.0251 IU/L(95% CI =0.0132,
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0.0371) per IQR increase of each pollutant(all p< 0.001). Innon-drinkers,
the PM10and CO exposures were not related to the ALT and AST levels
(all p> 0.05), whereas, in drinkers, significant associations between these
pollutants exposures and liver enzymes were observed (all p<
0.05).Chronic exposure to air pollution, especially PM10and CO, is
associated with an increase in liver enzyme levels, depending on alcohol
consumption, in the general adult population.
B0001
Presentation 19
The Effects of the Ethanol Extract from the Leaves of Malachra Capitate
L. and Lantana Camara L. on Mortality and Surface Structure of the
Culex Larvae
Saminee Suksumek, Weerawan Wonpuean, Patarapong Kroeksakul,
Phanom Sutthisaksopon and Arin Ngamniyom
Srinakharinwirot University, Thailand
Abstract—The Culex spp. are the vector-borne diseases that are widely
distributing throughout tropical countries including Thailand. These
mosquitoes are usually controlled and against by using insecticides.
However, it is concerned the pollutions of contaminations of those
chemicals in the environments leading to the negative impacts of the
human and animal health. Malachra capitate and Lantana camara are the
invasive weeds that are found in every region of Thailand. However, the
utilizations of those weeds for insect control have been evaluated.
Therefore, this study aims to investigate the effects of crude extracts from
the leaves of M. capitate and L. camara to the morality, mobility and
integuments of Culex larvae using a scanning electron microscopic
(SEM) and histological observation. In morality, 60% of lethal values and
abnormal mobility were found in the treatment of L. camara at 1 mg/L
for 24 hr. In SEM and histological analysis, the erosions and shrink of
integument and damaged cuticle layers were observed at 10 mg/L of M.
capitates, and 0.1 and 1 mg/L of L. camara crude extracts for 24 hr.
These results suggest that the crude extracts of L. camara may affect on
the morality, mobility and surface morphologies of Culex larvae.
B0109
Presentation 20
Neural Network-Based Discrimination of Golgi Type II Membrane
Proteins with Better Accuracy
Tatsuki Kikegawa, Kenji Etchuya and Yuri Mukai
Meiji University, Japan
Abstract—Type II membrane proteins in the Golgi apparatus play
important roles in biological functions, and predominantly exist as
catalysts related to post-translational sugar modification. This study
describes a new method for detecting Golgi-localized type II membrane
proteins (GLs) from post-Golgi type II membrane proteins (PGs), which
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are mainly localized in the plasma membrane and endoplasmic reticulum
(ER). The method is based on hydropathy profiles and the
position-specific scoring matrix (PSSM) in combination with the back
propagation artificial neural network (BP-ANN). The accuracy of
discriminating GLs from PGs was evaluated in a 5-fold cross-validation
test with 94.7% sensitivity and 93.5% specificity. This result shows that
our method can predict GLs with high accuracy, and that the PSSM and
BP-ANN
B0111
Presentation 21
Biological Network Analysis of Synthetic Pathogenicity Genes of
Atherosclerosis
Jhih-Ying Chen, Chia-Min Chen and Pei-Chun Chang
ASIA University, Taiwan
Abstract—Atherosclerosis is a chronic inflammatory process. In general,
it proceeded repeatedly to form a layer of plaque. Atherosclerotic plaques
are separated into two broad categories: stable and unstable. Stable
atherosclerotic plaque tends to be asymptomatic. Unstable plaques are
rich in macrophages and the extracellular matrix may separate from the
arterial lumen into circulation and eventually induce thrombus formation
in the lumen. This catastrophic event is called an infarction. One of the
most common recognized scenarios is called coronary thrombosis of
coronary artery, causing myocardial infarction. Therefore, early diagnosis
and administration of antithrombotic drugs is crucial to preventing
atherosclerosis, especially for unstable type of atherosclerotic lesions.
Synthetic lethality arises when a combination of mutations (or differential
expression) in two genes leads to cell death, whereas a mutation in only
one of these genes does not. We proposed a notion of synthetic
pathogenicity. Synthetic pathogenicity arises when a combination of
mutations (or differential expression) in two genes leads to disease,
whereas a mutation in only one of these genes does not. We isolate those
genes which locate on the hub with high connections in the network of
synthetic pathogenicity genes. These genes may be the drug targets for
Anti-thrombosis.
Dinner
19:00-20:30 Lounge in Front of C5
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Conference Venue
Global Front, Surugadai Campus, Meiji University, Tokyo, Japan
http://www.meiji.ac.jp/cip/english/about/campus/surugadai.html
Surugadai Campus is the traditional home to Meiji University. The campus is located in the
Kanda Surugadai area which, while retaining a strong atmosphere of a students’ town, is
located in proximity to Kasumigaseki, Otemachi, and other areas that are home to companies
and government ministries and agencies that are pivotal to Japan’s politics and economy. The
campus houses various educational and research facilities centering around the Liberty
Tower, which has become a landmark, and the Academy Common. In January 2013, the
Global Front was completed, where liberal arts graduate schools, research facilities,
Organization for the Strategic Coordination of Research and Intellectual Properties, and
Organization for International Collaboration are gathered under the concept of “a
state-of-the-art research cultivation hub that transmits to the world.”
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Map of Global Front and University Hall
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One Day Visit & Tour 09:30~17:30, January 20, 2018 (Saturday)
Assemble at APA Hotel Kanda Jimbocho Ekihigashi:09:30 — Visit Meiji University
Museum:10:00~11:00—Have Lunch in Local Restaurant:11:00~12:00—City Tour to Sensoji
Temple, Tokyo Sky Tree, The Tsukiji Market, and Odaiba Marin Park:12:30~17:30—Go Back
to APA Hotel Kanda Jimbocho Ekihigashi:17:30.
Based on the University's founding philosophy of "Rights, Liberty,
Independence and Self-Government", the Meiji University Museum
aims to publicly exhibit the achievements of its academic pursuits and to
also serve as an institution of learning. The Museum is divided into three
departments: The Commodity Department, the Criminal Materials
Department and the Archaeology Department.
Senso-ji Temple is located in Taito Ward, Tokyo, is Japan's existing
with "Edo style" of the public recreational land. According to legend,
the Empress Suiko Sanshiliunian, there are two fishermen fishing in
the Palace Togawa, picked up a gold one, 5.5 cm of Avalokitesvara, the
vicinity of people built a temple to raise funds dedicated to the Buddha
statues, and this is Senso-ji Temple.
Tokyo Sky Tree is a radio tower located in Sumida, Tokyo, Japan. The height
of 634.0 meters, in November 17, 2011 Guinness World Records certified as
"the world's tallest tower", becoming the tallest self-standing radio tower in
the world. It is also the second tallest building in the world after Burj Khalifa
in Dubai.
The Tsukiji Market is said that "Uogashi" or a riverside fish market
dates back to the 16th century, the beginning of the Edo period.
Tokugawa Ieyasu, the first Tokugawa shogun and builder of Edo as is
now Tokyo, invited fishermen from Tsukudajima, Osaka and gave them
a privilege for fishing in order to let them supply seafood to Edo Castle.
The fishermen purveyed fish to the Castle and sold the remains near the
Nihonbashi bridge. It was the origin of Uogashi.
Odaiba Marin Park is an artificial seaside park that can enjoy the
views of the Tokyo coastline. Swimming is prohibited here, but it’s
available for visitors play on the coast and reef, you can also enjoy the
windsurfing, overlooking the Rainbow Bridge across the street and
other beautiful scenery.
Notice: One lunch during the tour is covered, but the tour does not cover the entrance tickets. This
arrangement is temporary, The final agenda should be based on the actual one declared on the tour day.
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Note
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Note
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