A Perfusion-based Micro 3-D Cell Culture PlatformNTHU MSCLAdviser: C. H. LiuC. He Yeh

A Perfusion-based Micro 3-D Cell Culture PlatformOutlineCulture environment at large scaleCulture environment at micro scalePrincipleFabricationConclusion & FeaturesFurther work

General Culture Equipment (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) (12) (13) (14) (15) Lab On a Chip

On Chip AdvantagesSmall dimensionSaving sampleRapid assay process (micro dimension, avoid medium or reagent diffusion time gradient or concentration different )Cheap, disposable

On Chip Culture RequirementTemperature : 37pH : 7.2 ~ 7.4 ( 95% air 5%CO2 or HEPES)Buffered saline (PBS, PBSA, etc)Medium : (Serum, glucose, amino acids, vitamin, antibiotics )

3D culture (In vivo Culture)

A Perfusion-based Micro 3-D Cell Culture Platform

Working Principle :

A Perfusion-based Micro 3-D Cell Culture PlatformFabrication :

ConclusionFeature 1: 3D well-defined culture environmentmimic an in vivo environmentdistinct cellular behaviors (cell-cell and cell-extracellular interactions )provide more meaningful data

ConclusionFeature 2 : Short Loading cell channel

Feature 3 : High throughput (8 chambers)

Further workBioassay (inject reagent instead of culture medium in vivo bioassay)Biochemical experimentTissue engineeringDrug screening

TAS

ReferenceA PERFUSION-BASEDMICRO 3-D CELL CULTURE PLATFORM S. B. Huang1, M. H. Wu2, Z. F. Cui3, Z. Cui2, J. L. Lin4 and G. B. Lee1*

MICROFLUIDIC DEVICES FOR THE ASSEMBLY AND CULTURE OF THREE-DIMENSIONAL MULTI- CELLULAR CONSTRUCTS WITH DIFFUSION-LIMITED MICROENVIRONMENTS M. Pinelis1, R. W. Kasinskas2, R. T. Borno1, J. H. Park1, E. Chu3, N. S. Forbes2 and M. M. Maharbiz1

"Differentiation of human Embryonic Stem Cells into Hepatocytes in 2D and 3D culture system in vitro" The International Journal of Developmental Biology, Vol. 50, pp. 645-652, 2006

"The multilayered postconfluent cell culture as a model for drug screening" Critical Reviews in Oncology/Hematol, Vol. 36, pp. 147-157, 2000. J. M. Pardon; C.L. van der Wilt; K. Smid; E. Smitskamp-Wilms; H.H. Baukus; P. E. Piazo; G.

"Development of PDMS Microbioreactor with Well-defined and Homogenous Culture Environment for Chondrocyte 3-D Culture M. H. Wu, J. P. G. Urban, Z. Cui and Z. F. Cui, Biomedical Microdevices, Vol. 8, pp. 331-340, 2006.

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