abkürzungen - hs-mannheim.de · 5.1-mbpd industrial bioprocess development ibde 2 s, r 3/7 (3)...

Abkürzungen

BB Bachelor-Studiengang Biotechnologie BCB Bachelor-Studiengang Biologische Chemie BME Master-Studiengang Biotechnology (Englisch) BME-BPD Master BME mit Schwerpunkt Bioprocess Development BME-BST Master BME mit Schwerpunkt Biomedical Science and Technology

Abkürzungen in den Regelstudienplänen BB und BCB:

Für unterschiedliche Arten von Lehrveranstaltungen werden folgende Abkürzungen verwendet: L = Laborveranstaltung S = Seminar U = Übung V = Vorlesung

Für die Studienleistungen und Prüfungsleistungen werden folgende Abkürzungen verwendet: A = Anwesenheit Kxx = Klausurarbeit, Dauer xx Minuten LA = Laborarbeit Lxx = Laborprüfung, Dauer xx Minuten M = Mündliche Prüfung MA = Masterarbeit PA = Praktische Arbeit / Projektarbeit PB = Praktikumsbericht PP = Projektpräsentation PR = Präsentation PU = Pflichtübung R = Referat, Präsentation STA = Studienarbeit

Sonstige Abkürzungen: CR = Credits, Anrechnungspunkte, Kreditpunkte LV = Level MG / FG = Gewicht der jeweiligen Fachnote bzw. Modulnote bei der Bildung der Gesamtnote PL = Prüfungsleistung PLG = Gewicht der jeweiligen Prüfungsleistung bei der Bildung der Fachnote SWS = Semesterwochenstunden SL = Studienleistung

Akkreditierung 2017 Seite 3

Inhaltsverzeichnis

Modulhandbuch:

1. Regelstudienplan Bachelor-Studiengang Biotechnologie, BB……………………………… 5-7 2. Regelstudienplan Bachelor-Studiengang Biologische Chemie, BCB……………………… 8-10 3. Modulbeschreibungen für das erste Studienjahr BB und BCB…………………………..….11-25 4. Modulbeschreibungen für das 2.-4. Studienjahr BB……………………………………….….. 27-63 5. Modulbeschreibungen für das 2.-4. Studienjahr BCB…………………………………………. 65-83 6. Regelstudienplan Master-Studiengang Biotechnology, BME……………………………… 85-87 7. Modulbeschreibungen für BME mit Focus BPD……………………………………..……………89-113 8. Modulbeschreibungen für BME mit Focus BST…………………………………..………....…114-127


6. Regelstudienplan Master-Studiengang Biotechnology, BME

(1) Der Masterstudiengang MSc Biotechnology bietet zwei Schwerpunkte zur Wahl: BioprocessDevelopment (BPD) und Biomedical Science and Technology (BST).(2) Die Unterrichtssprache ist Englisch.(3) Der Gesamtumfang der für den erfolgreichen Abschluss des Studiums erforderlichenLehrveranstaltungen im Pflicht- und Wahlbereich beträgt in der Vertiefung BPD in den ersten beidenSemestern zusammen 46 Semesterwochenstunden und in der Vertiefung BST 42 Semester-wochenstunden. Dies entspricht 60 Anrechnungspunkte (CR) in der jeweiligen Vertiefungsrichtung. Mitder Masterarbeit werden insgesamt 90 Anrechnungspunkte (CR) erworben. Dabei entspricht einAnrechnungspunkt (CR) einer Arbeitsbelastung (work load) des Studierenden im Präsenz- undSelbststudium von 30 Stunden.(4) Die für den erfolgreichen Abschluss des Studiums erforderlichen Lehrveranstaltungen, sowie dieAnzahl der Studien- und Prüfungsleistungen im Pflicht- und Wahlpflichtbereich ergeben sich ausnachstehenden Tabellen.(5) In der Vertiefungsrichtung BPD kann anstelle der 10 CR-Praxis/Forschungsmodule "AdvancedAnimal Cell Technology" oder "Process Automation" ein optionales Forschungsmodul (ORME)gewählt werden. Um in der Säugetier-Zellkultur oder der Prozessoptimierung eine hohe Bandbreite anWissen vermittelt zu bekommen bzw. der spezifischen Neigung für bestimmte Themen zuentsprechen, kann nach Rücksprache und Genehmigung durch den Modulverantwortlichen und denStudiendekan dieses optionale Forschungsmodul innerhalb oder außerhalb der Hochschule von denStudierenden gewählt werden.In der Vertiefungsrichtung BST kann anstelle der 10 CR-Praxis/Forschungsmodule "Cell Science" oder "Advanced Bioanalytics" ein alternatives Forschungsmodul (ORME) gewählt werden. Um in der Angewandten Zellbiologie oder Bioanalytik eine hohe Bandbreite an Wissen vermittelt zu bekommen bzw. der spezifischen Neigung für bestimmte Themen zu entsprechen, kann nach Rücksprache und Genehmigung durch den Modulverantwortlichen und den Studiendekan dieses optionale Modul innerhalb oder außerhalb der Hochschule von den Studierenden gewählt werden. Für ein internes, optionales Forschungsmodul muss ein Professor der Hochschule die Betreuung und Benotung übernehmen. Bei externen Arbeiten muss zusätzlich ein lokaler Supervisor benannt werden. Die Benotung erfolgt durch den / die Betreuer mittels Bewertung eines schriftlichen Projektberichtes und einer Präsentation sowie einer wissenschaftlichen Diskussion. (6) Im Wahlmodul (Electives) sind jeweils drei Lehrveranstaltungen auszuwählen. Nicht alle deraufgeführten Lehrveranstaltungen sind jedes Semester verfügbar. Das gesamte Angebot wird vorVorlesungsbeginn in der Stundenplanung bekannt gemacht. Bei Fächern, die nur einmal im Jahrstattfinden, ist eine Wiederholungsklausur zum Ende des jeweiligen Semesters eingeplant, um denregulären Abschluss des 1. und 2. Semesters zu gewährleisten. Zusätzliche, fakultätsübergreifendeWahlfächer (Electives) können aus dem Master-Studienprogramm beispielsweise der FakultätVerfahrenstechnik (V) gewählt werden und bedürfen der Genehmigungspflicht durch denStudiendekan. Mit der Zeugnisbeantragung teilt der Studierende mit, welche der von ihm gewähltenLehrveranstaltungen als Wahlpflichtfächer ins Zeugnis aufgenommen werden. WeitereLehrveranstaltungen können auf Wunsch des Studierenden als Zusatzfächer im Zeugnis aufgeführtwerden, allerdings nur mit Ausweisung der erzielten Note.(7) Die Gesamtnote ergibt sich als gewichteter Mittelwert der Prüfungsleistungen. Gewichtsfaktor istder MG Wert.


Überschriften Abk. SL PL PLG CR MGModule / Lehrveranstaltungen 1 2 3

1-MBPD-BST Biostatistics BSTE 4 K120 1 5 52-MBPD Strain Development 6 6

2.1-MBPD Expression Systems EXSE 2 K90 1/2 (3)2.2-MBPD Metabolic Engineering MBEE 2 K90 1/2 (3)

3-MBPD Advanced Animal Technology *) 10 103.1-MBPD Cell Culture Process Development CPDE 2 K90 1/3 (4)3.2-MBPD Cell Culture Technology Lab CCTE 6 LA, S 2/3 (6)

4-MBPD Applied Biocatalysis LA, M 6 64.1-MBPD Enzyme Technology and Biocatalysis ENTE 2 (3)4.2-MBPD Enzyme Technology Lab ETLE 4 (3)

5-MBPD Process Development 7 75.1-MBPD Industrial Bioprocess Development IBDE 2 S, R 3/7 (3)5.2-MBPD Process Monitoring PRME 2 K90 4/7 (4)

6-MBPD Process Optimisation *) 10 106.1-MBPD Bioreaction Design BRDE 4 S, PA 1/2 (5)6.2-MBPD Advanced Bioreaction Engineering ABEE 4 S, PA 1/2 (5)

7-MBPD Downstream Processing in Biotechnology 7 77.1-MBPD Protein Downstream Processing PDPE 2 K90 1/3 (3)7.2-MBPD Downstream Processing Lab DPLE 4 LA, R 2/3 (4)

8-MBPD-BST Electives 9 9 select three courses from **) **) 2 **) 1/3 (3)

**) 2 **) 1/3 (3)**) 2 **) 1/3 (3)

9-MBPD-BST Final Research Project MA, R, M 1 30 309.2-MBPD-BST Research Seminar RSEE9.1-MBPD-BST Master Thesis MTHE

Summen 24 22 90 90


10-MBPD Optional Research Module ORME PA, R, M 1 10 10

FOCUS: Bioprocess Development (BPD)SWS

*) Course Replacement: Um in der Säugetier-Zellkultur oder der Prozessoptimierung eine hohe Bandbreite an Wissen vermittelt zu bekommen bzw. der spezifischen Neigung für bestimmte Themen zu entsprechen, kann nach Rücksprache und Genehmigung durch den Modulverantwortlichen und den Studiendekan alternativ ein optionales Forschungsmodul (ORME) innerhalb oder außerhalb der Hochschule von den Studierenden gewählt werden. Dies kann alternativ das Modul "Advanced Animal Cell Technology" oder das Modul "Process Optimisation" ersetzen. Innerhalb des Studienprogrammes kann nur ein optionales Forschungsmodul durchgeführt werden.

Module No.

Modul No.

*) Course Replacement BME-BPDSWS

8

Course Replacement: In order to acquire a wide spectrum of knowledge in mammalian cell culture technology or in process optimization, respectively, and to gain insights into fields of particular interest, an optional research module (ORME) can be chosen. The ORME module has to be approved by the module coordinator and the MSc program coordinator (Studiendekan) and can be performed in house or outside the university. An approved optional research module replaces either the module "Advanced Animal Cell Technology" or "Process Optimisation", respectively. No more than one optional module can be taken during the study program.



1-MBPD-BST Biostatistics BSTE 4 K120 1 5 52-MBST Biomedical Science 6 6

2.1-MBST Human Physiology HPYE 2 K90, PA 1/2 (3)2.2-MBST Molecular Medicine MOME 2 K90 1/2 (3)

3-MBST Cell Science *) 10 103.1-MBST Cell Physiology CPHE 2 K90 1/3 (3)3.2-MBST Cell Based Assays Lab CBAE 6 LA,R 2/3 (7)

4-MBST Pharmacology K120 1 6 64.1-MBST Pharmacokinetics PHKE 2 (3)4.2-MBST Pharmacodynamics PHDE 2 (3)

5-MBST Bioinformatics and Proteomics 7 75.1-MBST Bioinformatics BINE 2 K90 3/7 (3)5.2-MBST Proteomics PROE 2 K90 4/7 (4)

6-MBST Advanced Bioanalytics *) 10 106.1-MBST Tissue Analytics TSAE 2 R, S 1/3 (3)6.2-MBST Advanced Bioanalytics Lab ADBE 6 LA, R 2/3 (7)

7-MBST Biosensing and Analytical Technologies 7 77.1-MBST Biosensors BISE 2 K90 3/7 (3)7.2-MBST Modern Analytical Methods MAME 2 K90 4/7 (4)

8-MBPD-BST Electives 9 9 select three courses from **) **) 2 **) 1/3 (3)

**) 2 **) 1/3 (3)**) 2 **) 1/3 (3)

9-MBPD-BST Final Research Project MA, R, M 1 30 309.2-MBPD-BST Research Seminar RSEE9.1-MBPD-BST Master Thesis MTHE

Summen 22 20 90 90


10-MBST Optional Research Module ORME PA, R, M 1 10 10

Module No.

Modul No.

FOCUS: Biomedical Science and Technology (BST)SWS

*) Course Replacement BME-BSTSWS

8

Course Replacement: In order to acquire a wide spectrum of knowledge in advanced cell technology or in bioanalytics, respectively, and to gain insights into fields of particular interest, an optional research module (ORME) can be chosen. The ORME module has to be approved by the module coordinator and the MSc program coordinator (Studiendekan) and can be performed in house or outside the university. An approved optional research module replaces either the module "Cell Science" or "Advanced Bioanalytics", respectively. No more than one optional module can be taken during the study program.

*) Course Replacement: Um in der fortgeschrittenen Zelltechnologie oder Bioanalytik eine hohe Bandbreite an Wissen vermittelt zu bekommen bzw. der spezifischen Neigung für bestimmte Themen zu entsprechen, kann nach Rücksprache und Genehmigung durch den Modulverantwortlichen und den Studiendekan alternativ ein optionales Forschungsmodul (ORME) innerhalb oder ausserhalb der Hochschule von den Studierenden gewählt werden. Dies kann alternativ das Modul "Cell Science" oder das Modul "Advanced Analytics" ersetzen. Innerhalb des Studienprogrammes kann nur ein optionales Forschungsmodul durchgeführt werden.


8.1-MBPD-BST Entrepreneurship EPRE 2 S, R 1 3 38.2-MBPD-BST Drug Development DDTE 2 S, R 1 3 38.3-MBPD-BST Clinical Chemistry (SS) CCHE 2 K90 1 3 38.4-MBPD-BST Immunology IMUE 2 K90 1 3 38.5-MBPD-BST Environmental Biotechnology (WS) EBTE 2 K90, LA 1 3 38.6-MBPD-BST Plant Biotechnology (SS) PBTE 2 K90 1 3 38.7-MBPD-BST Transgenic Animals in Medicine (SS) TGAE 2 K90 1 3 38.8-MBPD-BST Human Physiology (WS) HPYE 2 K90, PA 1 3 38.9-MBPD-BST Pharmacodynamics PHDE 2 K90 1 3 3

8.10-MBPD-BST Pharmacokinetics PHKE 2 K90 1 3 38.11-MBPD-BST Proteomics PROE 2 K90 1 3 38.12-MBPD-BST Modern Analytical Methods MAME 2 K90 1 3 38.13-MBPD-BST Bioinformatics BINE 2 K90 1 3 38.14-MBPD-BST Expression Systems EXSE 2 K90 1 3 38.15-MBPD-BST Metabolic Engineering MBEE 2 K90 1 3 38.16-MBPD-BST Biosensors BISE 2 K90 1 3 3

Module No.

**) Not all the electives listed are offered every semester. Four weeks prior to the start of the semester the faculty will announce which elective courses are available. Other interfaculty electives from department V (Process Engineering; Master Program) must be approved by the supervisory committee.

**) Electives for BME-BPD and BME-BSTSWS


7. Modulbeschreibungen für den Master BME mit Focus BPD

Module 1-MBPD-BST: Biostatistics

Code: BSTE

Module coordinator: Prof. Dr. Gerhard Rufa

Lecturer: Prof. Dr. Gerhard Rufa Place in the curriculum: Mandatory course in the first semester BPD and BST, Level 4 Language: English Teaching form: Lecture and tutorial, 4 teaching units Work load: 60 hours course time and 90 hours personal learning including tutorial Credit points: 5 Prerequisites acc. to PO: None Recommended prerequisites: Fundamentals of Biostatistics

Assessment: Written examination, 120 min (K120)

Learning objectives: The students • are familiar with random experiments, random and pivotal variables and their probability distributions.• are able to determine quantiles of estimators and testing statistics based on the corresponding sampling

distributions.• learn to apply the linear and quadratic regression model.• understand the concepts of statistical inference: point and interval estimation, hypothesis testing.• learn to formulate hypotheses and to perform significance tests.• are able to calculate estimates of population parameters.• can calculate one- and two-sided confidence intervals for population parameters.• are familiar with correlation and regression analysis.• learn to perform inferences about normally and binomially distributed populations.• can calculate minimal sample sizes regarding estimation and hypothesis testing.• are able to design biomedical studies.

Course content: • Basic model of system and data analysis• Regression• Sampling distributions• Point estimates• Confidence Intervals•Normal regression analysis• Inferences about normally and binomially distributed populations•Nonparametric and distribution-free methods•Multivariate methods• Biostatistical design of medical studies

Resources and literature: • Lecture notes and series of exercises• L.D. Fisher, G. van Belle: Biostatistics• R.A. Fisher: Statistical Methods and Scientific Inference


Module 2-MBPD: Strain Development

Code: EXSEE and MBEE

Module coordinator: Prof. Dr. Matthias Mack

Course 2.1-MBPD: Expression Systems (EXSE)

Lecturer: Prof. Dr. Petra Kioschis Place in the curriculum: Mandatory course in the first semester BPD, Level 4

Elective course in the first or second semester BST, Level 4 Language: English Teaching form: Lecture, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: None


Learning objectives: The students • acquire an understanding of different genetic systems, their organization and expression mechanisms.• achieve comprehensive knowledge of heterologous expression systems in prokaryotes and eukaryotes.• gain advanced knowledge of DNA regulatory elements and different recombinant expression vectors.• understand applications of protein expression systems in research in contrast to industrial production.• develop competency in manipulation of host systems and creation of a production line.• gain learning experience that would integrate the understanding of genetic systems with specific

requirements of protein synthesis research and industrial applications.

Course content: • Introduction to expression systems – bacterial, yeast, insect and mammalian cells.•DNA regulatory elements.• Properties of prokaryotic and eukaryotic / integrated expression vectors.• Prokaryotic expression systems: Features of E. coli expression.• Protein expression systems in yeast: Saccharomyces cerevisiae and Pichia pastoris.• Insect cells - Baculovirus expression systems.• Protein expression systems in mammalian cells (non-inducible / inducible)• Expression systems for production of biopharmaceuticals

Resources and literature: • Recombinant Gene Expression. Reviews and Protocols. Editors: Paulina Balbás, Argelia Lorence Humana

Press; 3rd ed. 2012 edition, ISBN: 978-1617794322•Gene Control, David Latchman, Garland Science (2010), ISBN-10: 0815365136• Production of Membrane Proteins: Strategies for Expression and Isolation. Anne Skaja Robinson,

Wiley-VCH; 1 edition (2011), ISBN-10: 3527327290• Protein Expression in Mammalian Cells: Methods and Protocols. James L Hartley, Humana Press, 2012,

ISBN-10: 1617793515• Production of recombinant proteins: microbial and eukaryotic expression systems. Gellissen, G. (ed.)

Wiley-VCH, Weinheim, Germany; 1 edition (February 11, 2005)• Therapeutic Proteins. Methods and Protocols. Voynov, Vladimir, Caravella, Justin A., Humana Press

(2012), ISBN 978-1-61779-921-1• Students are referred to published journal papers throughout the course.


Course 2.2-MBPD: Metabolic Engineering (MBEE)

Lecturer: Prof. Dr. Matthias Mack Place in the curriculum: Mandatory course in the first semester BPD, Level 4

Elective course in the first or second semester BST, Level 4 Language: English Teaching form: Lecture, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: None


Learning objectives: The students • are familiar with the basic concepts of metabolic engineering.• understand how massively parallel techniques (“-omics”) contribute to metabolic engineering.• understand the complex biochemistry of a microbial cell.• understand the different concepts of regulation of gene expression in prokaryotic bacteria.• know how microbial strains can be engineered in order to overproduce a desired compound (bulk

chemicals and fine chemicals).• are familiar with a variety of different production processes which have been optimized using metabolic

engineering.

Course content: • Principles of metabolic engineering.•Genome, transcriptome, proteome and metabolome studies as prerequisites for metabolic engineering.• The complexity of bacterial metabolism.• Regulation of bacterial metabolism.• The stable introduction of heterologous genes in microorganisms.• Case studies:

- Biotechnological synthesis of vitamin C using Erwinia herbicola.- Biotechnological synthesis of riboflavin (vitamin B2) using the bacterium Bacillus subtilis and the

fungus Ashbya gossypii.- Synthesis of the riboflavin analog roseoflavin in Streptomyces davawensis.- Synthesis of other antibiotics employing Streptomycetes.- Lactic acid synthesis using the yeast Kluyveromyces lactis.- Biomass to fuels.- Biopolymer production.- Reduction of diacetyl during ethanol fermentation employing Saccharomyces cerevisiae.- Production of single cell protein (SCP).- Synthesis of amino acids using Corynebacterium glutamicum.

Resources and literature: • Stephanopoulos, G. N., Aristidou, A. A., Nielsen, J. “Metabolic Engineering: Principles and

Methodologies". San Diego: Academic Press•Gottschalk, G., “Bacterial Metabolism”. Berlin: Springer•Quizzes.•Original publications in the field of metabolic engineering and regulation of bacterial metabolism.


Module 3-MBPD: Animal Cell Technology

Code: CPDE and CCTE

Module coordinator: Prof. Dr. Philipp Wiedemann

Course 3.1-MBPD: Cell Culture Process Development (CPDE)

Lecturer: Prof. Dr. Philipp Wiedemann Place in the curriculum: Mandatory course in the first semester BPD, Level 4 Language: English Teaching form: Lecture, 2 teaching units Work load: 30 hours course time and 90 hours personal learning Credit points: 4 Prerequisites acc. to PO: None Recommended prerequisites: Background in cell biology, molecular genetics and engineering

principles


Learning objectives: The students • know the different concepts of biopharmaceuticals and conventional pharmaceuticals. • understand the combination and importance of genetic engineering, cell biology and process technology

for biopharmaceutical process development and production. • can name the needs and problems of a cell culture production process. • know the critical parameters for characterizing an animal cell culture process. • can assess the importance of early process design for large scale applications. • understand the implication of upstream process parameters for harvest and downstream. • can fit a biopharmaceutical process and its development in the larger setting of plant design and

economics.

Course content: • Classes of biopharmaceuticals • Mammalian cell line development and cell banking • Cell metabolism and its implication for cell culture processes • Cultivation parameters of mammalian cells • Metabolic engineering of mammalian cells for process optimization • Media design for cultivation, media strategies for large scale production • Cell culture bioreactor choice and operation • Process design and scale-up • Upscale parameters for animal cell culture processes • Overview over harvest and downstream technology • Large scale production and plant design aspects, economic considerations

Resources and literature: • Walsh G; Biopharmaceuticals; 2nd edition Repr., Wiley, 2006 • Freshney I; Culture of Animal Cells; 5th edition Wiley, 2005 • Hu WS; Cell Culture Bioprocess Engineering; http://www.cellprocessbook.com/, 2112 • Al-Rubeai M; Cell Engineering Vol. 9: Animal Cell Culture; Springer, 2015 • Review articles and research papers as appropriate


Course 3.2-MBPD: Cell Culture Technology Lab (CCTE)

Lecturer: Prof. Dr. Philipp Wiedemann Place in the curriculum: Mandatory course in the first semester BPD, Level 4 Language: English Teaching form: Laboratory and Seminar, 6 teaching units Work load: 90 hours course time and 90 hours personal learning Credit points: 6 Prerequisites acc. to PO: None Recommended prerequisites: Background in cell culture techniques and cell biology

Assessment: Laboratory Assessment (LA) and Seminar (S)

Learning objectives: The students • apply cell cultivation techniques to relevant questions of cell culture process design.• obtain a first understanding of the structure and organisation of development projects by themselves

being involved in organising relevant parts of the course.• strengthen their skills of carrying out temporally focused laboratory projects.• experience the large range of topics dealt with when designing a cell culture process in a hands-on

fashion.• analyze and interpret their experimental data in the light of up-to-date scientific information.• defend their results and discuss them with their peers.• are involved in current research activities of the institute.

Course content: • Culture of mammalian cells in different process relevant cultivation systems•Development of a stock culture scheme•Determination of cell and metabolic culture parameters of mammalian cells• Small-scale product formation in mammalian cells, process monitoring and product titer determination• Introduction to cell culture bioreactor operation, process control and data acquisition• Assessment of the influence of metabolic engineering of the apoptotic pathway on cell culture

parameters• Flow cytometric analysis of mammalian cells with respect to apoptosis• Additional contents as appropriate with respect to current research activities of the institute

Resources and literature: •Handout Recombinant Protein Production, Hochschule Mannheim•Walsh G; Biopharmaceuticals; 2nd edition Repr., Wiley, 2006• Freshney I; Culture of Animal Cells; 5th edition Wiley, 2005•Hu WS; Cell Culture Bioprocess Engineering; http://www.cellprocessbook.com/, 2112• Al-Rubeai M; Cell Engineering Vol. 9: Animal Cell Culture; Springer, 2015• Review articles and research papers as appropriate


Module 4-MBPD: Applied Biocatalysis

Code: ENTE and ETLE

Module coordinator: Prof. Lasse Greiner

Course 4.1-MBPD: Enzyme Technology and Biocatalysis (ENTE)

Lecturer: Prof. Dr. Lasse Greiner Place in the curriculum: Mandatory course in the first semester BPD, Level 4 Language: English Teaching form: Lecture, 2 teaching units Work load: 30 hours course time and 30 hours personal learning Credit points: 2 Prerequisites acc. to PO: None Recommended prerequisites: None

Assessment: Oral examination (M) combined with ETLE

Learning objectives: The students ● know the potential of enzyme catalysis in industrial biotechnology.● know exemplary industrial processes.● understand how to derive an adsorption rate law from a hypothesis.● know the main influence factors affecting enzymatic rate.● are familiar with the basics of enzyme immobilisation methods.• understand screening approaches for finding and improving industrial enzymes.

Course content: ● Structure of enzymes● Enzyme classes● Enzyme assays● Enzyme kinetics and data interpretation● Enzymes with incomplete selectivity: E-value● Kinetic racemic resolution● Dynamic kinetic racemic resolution● Process stability● Non-conventional reaction media● Enzyme immobilisation● Configuration and choice of enzyme reactors● Immobilized enzyme reactors (PFR, CSTR)● Implications of mass transfer

Resources and literature: ● Klaus Buchholz, Volker Kasche and Uwe T. Bornscheuer: Biocatalysts and Enzyme Technology, Wiley-VCH-

Verlag, 2005.● Andreas S. Bommarius und Bettina R. Riebel: Biocatalysis, Wiley-VCH-Verlag, 2004.● Levenspiel, O., Chemical Reaction Engineering● Doran, P. M., Bioprocess Engineering Principles, 2nd edition● Liese, A., Seelbach, K., Wandrey, C., Industrial biotransformations● Lecture notes● Various resources as deposited in moodle platform: Research articles, notes etc.


Course 4.2-MBPD: Enzyme Technology Lab (ETLE)

Lecturer: Prof. Dr. Lasse Greiner Place in the curriculum: Mandatory course in the first semester BPD, Level 4 Language: English Teaching form: Lecture, 4 teaching units Work load: 60 hours course time and 60 hours personal learning Credit points: 4 Prerequisites acc. to PO: None Recommended prerequisites: None

Assessment: Laboratory Assessment (LA) and oral examination (M), with ENTE

Learning objectives: The students ● are able to characterize an enzyme with respect to a process. ● plan kinetic experiments with simulation or design of experiments tools. ● know how to characterize different enzyme reactor types. ● are able to run lab-scale continuous reactions (enzyme membrane reactor or fluidised bed). ● are able to carry out enzyme immobilisation (adsorption or covalent binding). ● are able to determine the operational stability and the space-time yield of a biocatalytic process.

Course content: ● Initial rate determination ● Covalent and adsorptive enzyme immobilization ● Determination of kinetics and fitting of a suitable kinetic model for the free and immobilised enzyme. ● Simulation of a continuous reaction and performing the reaction according to the simulation. ● Commissioning of different enzyme reactors (membrane reactor, packed-bed reactor and CSTR). ● Characterization of enzymatic reactors concerning space time yield, turn over number, residence time

and turnover frequency.

Resources and literature: ● Klaus Buchholz, Volker Kasche and Uwe T. Bornscheuer: Biocatalysts and Enzyme Technology, Wiley-VCH-

Verlag, 2005. ● Andreas S. Bommarius und Bettina R. Riebel: Biocatalysis, Wiley-VCH-Verlag, 2004. ● Levenspiel, O., Chemical Reaction Engineering ● Doran, P. M., Bioprocess Engineering Principles, 2nd edition ● Liese, A., Seelbach, K., Wandrey, C., Industrial biotransformations ● Script of the course. ● Various resources as deposited in moodle: Research articles, notes etc.


Module 5-MBPD: Process Development

Code: IBDE and PRME

Module coordinator: Prof. Thomas Beuermann

Course 5.1-MBPD: Industrial Bioprocess Development (IBDE)

Lecturer: Prof. Dipl.-Ing. Michael Kopf Place in the curriculum: Mandatory course in the second semester BPD, Level 4 Language: English Teaching form: Lecture, 2 teaching units Work load: 30 hours course time and 90 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: BSc.-Level Basics in Process Engineering, Physical Chemistry,

Mechanical processing

Assessment: Seminar (S) and presentation (R)

Learning objectives: The students • gain insight into the workflow of large-scale (tons/year) industrial bioprocess development.• learn how to capture and build-up industrial process knowledge.• can combine theoretical understanding with practical applications related to relevant industrial systems,

product design and manufacture.• become familiar with processes and techniques to manipulate materials to meet industrial technology

and product design challenges.• understand the appropriateness of products, safety as well as economical aspects.• are trained to study and assess different development strategies and experiences critically.

Course Content: • Process to develop new or alternative production process for products of interest:

ideation process, basic concept, critical analysis, development steps• Value proposition of novel product / process:

quality, performance, price,.eco-efficiency, regional aspects• Critical aspects along the development process:

feedstock issues, design to cost of production (CoP), quality and performance, regulatory issues,eco-efficiency (raw material and energy efficiency), LCA• From lab to production (focus of lecture):

phases of a development process (explorative research, proof of principle, proof of concept, scale-up andapparatus design, plant design, production, operational excellence)• Competitor intelligence :

competitors with their “own” processes, alternative products, similar in application• Benchmarking as a development tool:

cost benchmarking, CoP as a development tool to identify optimization potential• Production scenarios:

own investment, toller, production partner

Resources and literature: • Primary literature• Case studies


Course 5.2-MBPD: Process Monitoring (PRME)

Lecturer: Prof. Dr. Thomas Beuermann Place in the curriculum: Mandatory course in the second semester BPD, Level 4 Language: English Teaching form: Lecture, 2 teaching units Work load: 30 hours course time and 90 hours personal learning Credit points: 4 Prerequisites acc. to PO: None Recommended prerequisites: None


Learning objectives: The students •will understand the operation principle of the most commonly used sensors in biotechnology.• can explain the working principle of a closed-loop control of a bioreactor.•will know the time response of a controlled system.• can balance the advantages and disadvantages of different measuring principles and sensors.• are familiar with the most important sensors and analytical setups for bioprocess monitoring.• gain advanced knowledge of the application potential of optical spectroscopy in biotechnology.

Course content: • Sensor signals, measurement data acquisition, analog-to-digital converter• Sensors and instruments: resistor temperature devices, thermo couples, differential pressure cells,

volume and mass flow meters (Vortex, Orifice, Rotameter, electromagnetic, ultrasonic, Coriolis), fillinglevel sensors (ultrasonic, microwave, hydrostatic pressure, uplift of a displacer, capacitive, load cell),amperometric oxygen sensor

•Optical spectroscopy (UV/VIS, NIR, MIR, Raman, fluorescence, scattering of light) and optical sensors formeasuring CO2, O2, pO2, cell density, sugar, ethanol, culture fluorescence

• Bioprocess monitoring, on-line carbon balance of yeast fermentations using optical sensors• Control loops and control theory, time response of a controlled system, methods for optimizing a closed-

loop system (CHR, Ziegler-Nichols)• Tank system with level control (with Lab Course)

Resources and literature: •Geörg, D., Schalk, R., Methner, F.-J., Beuermann, T.: MIR-ATR sensor for process monitoring, Meas. Sci.

Technol. 26 (2015) 065501 (11pp).• Beuermann, T., Egly, D., Geörg, D., Klug, K.I., Storhas, W., Methner, F.-J.: On-line carbon balance of yeast

fermentations using miniaturized optical sensors, J. Biosci. Bioeng., 113(3), 399-405 (2012).• Egly, D., Geörg, D., Rädle, M., Beuermann, T.: A compact multi-channel fluorescence sensor with ambient

light suppression, Meas. Sci. Technol. 23 (2012) 035702 (8pp).• Beuermann, T., Egly, D.: „MIR-ATR-Spectroscopy in combination with multivariate methods for

fermentation control and determination of intracellular yeast constituents”, Proceedings of the 23rd VH-Yeast Conference, April 26-27, 2010 in Vienna, 19-38.

• Lecture notes (Moodle)


Module 6-MBPD: Process Optimisation

Code: BRDE and ABEE Module coordinator: Prof. Dr. Karlheinz Preuß

Course 6.1-MBPD: Bioreaction Design (BRDE)

Lecturer: Prof. Dr. Karlheinz Preuß Place in the curriculum: Mandatory course in the second semester BPD, Level 4 Language: English Teaching form: Lecture, 4 teaching units Work load: 60 hours course time and 90 hours personal learning Credit points: 5 Prerequisites acc. to PO: None Recommended prerequisites: None

Assessment: Seminar (S) and independent project work (PA)

Learning objectives: The students will learn how to • develop, program and check numerical models of bioreactors.• apply Good Engineering Practice as quality assurance method for engineering calculations.• use numerical models for the aim of parameter identification and process optimisation.

Course content: • General procedure to derive (partial) mass balances for typical process equipment• Program partial mass balances in mathematical calculation software• Verifying calculations by means of design specification, code review and software test• Modelling bioreactions and their kinetics• Building mass balance based models for not ideally mixed types of bioreactor• Identifying parameters of bioreactions (e.g. specific reaction rates and yield coefficients)• Optimizing the performance of bioreactors using process models and optimisation methods

Resources and literature: • Lecture slides• Exercises with solutions and comments• Software tutorials


Course 6.2-MBPD: Advanced Bioreaction Engineering (ABEE)

Lecturer: Prof. Dr. Karlheinz Preuß and Prof. Dr. Lasse Greiner Place in the curriculum: Mandatory course in the second semester BPD, Level 4 Language: English Teaching form: Lecture, 4 teaching units Work load: 60 hours course time and 90 hours personal learning Credit points: 5 Prerequisites acc. to PO: None Recommended prerequisites: None

Assessment: Seminar (S) and independent project work (PA)

Learning objectives: The students will learn how to • make use of scientific publications for the development of bioreactions and for the design of process

control applications.• employ simulation and optimisation methods for process development and scale-up.• carry out experimental planning and model choice for bioreactions.

Course content: • General approach to model and simulate processes with multiple parallel bioreactions• Methods, applications and challenges of using exhaust gas analysis for process analysis and control• Advanced bioprocess control (state estimation, SoftSensors, adaptive model based feedback control)• Practical implementation and GMP validation of process control software• Experimental planning using design software and optimisation algorithms• Data evaluation and experimental planning excercises for model discrimination

Resources and literature: • Lecture slides• Case studies• Software tutorials


Module 7-MBPD: Protein Downstream Processing

Code: PDPE and DPLE

Module coordinator: Prof. Dr. Christian Frech

Course 7.1-MBPD: Protein Downstream Processing (PDPE)

Lecturer: Prof. Dr. Christian Frech Place in the curriculum: Mandatory course in the second semester BPD, Level 4 Language: English Teaching form: Lecture, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: None


Learning objectives: The students • acquire detailed knowledge and skills to deal with diverse and complex processes and products that exist

in bio-manufacturing and an essential understanding of the range of technology and techniques availableto support this activity.

• develop a critical understanding of the relationships and interactions between the various components ina bioprocess system to achieve the overall goal of successful downstream processing.

• develop and use a significant range of science and engineering skills, techniques and practices in proteindownstream processing.

• critically review existing practice and develop original and creative solutions to problems within the fieldof protein purification.

Course content:

• Introduction to downstream separation techniques• Influence of product type on separations•Methods for in-vitro refolding of recombinant proteins• Chromatographic techniques used for protein purification• Applications and case studies• Performance Assessment and Case Studies

Resources and literature: •Handout• Industrial Bioseparations: Principles and Practice; Daniel Forciniti (2007) ISBN 978-0-8138-2085-9 - Wiley

& Sons• Bioseparations Engineering: Principles, Practise, and Economics; Michael R. Ladisch (2001) ISBN 978-0-

471-24476-9 - Wiley & Sons 2001• Protein Chromatography: Process Development and Scale-Up; Carta & Jungbauer (2010) ISBN 978-3-527-

31819-3 - Wiley-VCH, Weinheim


Course 7.2-MBPD: Downstream Processing Lab (DPLE)

Lecturer: Prof. Dr. Christian Frech Place in the curriculum: Mandatory course in the second semester BPD, Level 4 Language: English Teaching form: Teaching Lab, 4 teaching units Work load: 60 hours course time and 60 hours personal learning Credit points: 4 Prerequisites acc. to PO: None Recommended prerequisites: None

Assessment: Laboratory Assessment (LA) and Presentation (R)

Learning objectives: The students • acquire detailed knowledge and skills to deal with diverse and complex processes and products that exist

in bio-manufacturing and an essential understanding of the range of technology and techniques availableto support this activity.

Course content: •Multi-step purification of a monoclonal antibody from clarified cell culture supernatant using

chromatography• In-vitro oxidative refolding of lysozyme

Resources and literature: • Laboratory notes


Module 8-MBPD-BST: Electives in the Master BPD and BST

Code: ELCE - see individual elective

Module coordinator: Prof. Petra Kioschis

Course 8.1-MBPD-BST: Entrepreneurship (EPRE)

Lecturer: Prof. Dr. Karin Arregui Place in the curriculum: Elective course in the first or second semester BPD and BST, Level 4 Language: English Teaching form: Lecture and seminar, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: None

Assessment: Seminar and presentation (R)

Learning objectives: The students will acquire knowledge of • the generation of a basic understanding of intellectual property, innovation, and economic

value creation e.g. from academic research results.• the development of a viable and compelling business idea.• the presentation of the business idea in front of a critical audience.

Course content: • Creating a business model using the Business Model Canvas/ Lean Canvas:

Problem, Customer Segments, Unique Value Proposition, Solution, Distribution Channels, RevenueStreams, Cost Structure, Key Metrics, Unfair Advantage; Customer Relationships, Key Partners, KeyResources, Key Activities

•Different sources of capital for funding a startup in different stages (debt versus equity; Angels, VCs,crowd-funding platforms)

• Structure and design of a pitch presentation• Participants are grouped in founder teams to work jointly on business ideas. Each founder team will

receive a current research result/article/patent about an invention in the field of life sciences or healthtechnology as a basis for a business idea and will pitch the business idea.

Resources and literature: •Maurya, Ash. Running Lean. 2nd ed. O’Reilly and Associates, 2012.• Blank, Steve and Dorf, Bob. The Startup Owner’s Manual: The Step-by-Step Guide for Building a Great

Company, K&S Ranch, 2012.• Yock, Paul et al. Biodesign. 2nd ed. Cambridge University Press, 2012.


Course 8.2-MBPD-BST: Drug Development (DDTE)

Lecturer: Prof. Dr. Karin Arregui Place in the curriculum: Elective course in the first or second semester BPD and BST, Level 4 Language: English Teaching form: Lecture and seminar, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: None

Assessment: Seminar and presentation (R)

Learning objectives: The students • acquire a comprehensive overview of the complex process of bringing a drug to the market.•will be aware of the interdependence of the different disciplines in the drug development process.• learn strategies for effective and efficient drug development.•will be able to identify future fields of interest.

Course content: This course provides an overview over the drug development process, covering • preclinical drug development• clinical development•manufacturing•market application

Further topics include topics such as: •medical device development• drug delivery systems• digital health• biobanks and personalized medicine• pharmaceutical project management• pharma marketing• business development• quality management• trends in the pharmaceutical and biotechnological industries.

Resources and literature: •Ng, Rick. Drugs: From Discovery to Approval. 2nd ed. Wiley-Blackwell, 2008• Friedhoff, Lawrence. New Drugs: An Insider’s Guide to the FDA’s New Drug Approval Process. PSPG

Publishing, 2015• Fischer, Dagmar, Breitenbach, Joerg. Die Pharmaindustrie. 4th ed. Spektrum Akademischer Verlag, 2012


Course 8.3-MBPD-BST: Clinical Chemistry (CCHE)

Lecturer: Prof. Dr. Tobias Werner Place in the curriculum: Elective course in the first or second semester BPD and BST, Level 4 Language: English Teaching form: Lecture and seminar, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: Lecture Human Physiology (HPYE)

Assessment: Written examination, 90 min, (K90)

Learning objectives: The students • understand the basics of the issued disease related pathobiochemical pathways and can conclude the

needs for the analytical processes.• understand basic principles of pre-analytical treatment.• understand simple strategies of stepwise laboratory diagnostics.• can describe context between disease and measured disease markers.• can distinguish needs that are required to develop methodologies for novel tests and assays.

Course content: • Fundamentals of instrumentation and basic methodology in the clinical chemistry laboratory• Basic principles and methods such as water balance, acidosis/alkalosis, blood buffers and gases, and

electrolytes, pre-analytical treatment and point of care testing.•Disorders of carbohydrate and lipid metabolism and methods of diagnosis of different types of diabetes

mellitus (glucose, c-peptid und HbA1c); understanding of the glucose homeostasis• combination of medical disorders that increase the risk of developing cardiovascular disease and diabetes

(metabolic syndrome and insulin resistance)• Renal disease and test strip methods for urine diagnosis• Proteins as marker for diseases and methods for diagnosis of proteinuria (protein-electrophoresis and

immunfixation etc.)• Laboratory diagnostics of acute coronary syndrome (creatine kinase, myoglobin and troponin)• Laboratory diagnostics of common infectious diseases (viral vs. bacterial)• Possibly - study trip for laboratory visit (e. g. Roche-Diagnostics)

Resources and literature: • C.A. Burtis, E.R. Ashwood, D.E. Bruns, Tietz Fundamentals of Clinical Chemistry, A Saunders Title 6. Edition

(2007)• Lecture notes


Course 8.4-MBPD-BST: Immunology (IMUE)

Lecturer: Prof. Dr. Roswitha Stenzel Place in the curriculum: Elective course in the first or second semester BPD and BST, Level 4 Language: English Teaching form: Lecture and seminar, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: None


Learning objectives: The students will be able to • describe the various elements of immune system responses.• describe the sequence of events during inflammation.• explain how the elements of the immune system interact in protecting the body against bacterial and

viral infection.• describe how the immune system may be manipulated by vaccination and explain how this results in

protection against infectious disease.• describe the mechanisms and consequences of the malfunctioning immune system.• apply different components of the immune system (e.g. cytokines and antibodies) in therapy.

Course content: • Cells, organs,• Structure of antibodies, generation of antibody diversity• Complement system• Cell-mediated immunity (T-cells, MHC, Antigen-presenting cells)• Cytokines• Innate immune system• Leucocyte activation and migration, chemokines• Cancer and the immune system and antibody therapy• Vaccination• Transplantation• AIDS•Hypersensitivity• Antibody Drug conjugates• Autoimmune Diseases

Resources and literature: • Immunology, Roitt, Brostoff, Male, 7.ed., 2012,Mosby• Janeway `s Immunobiology, Murphy, Travers, Walport, 8.ed., 2016, Garland Science• Kuby Immunology, Owen, Punt, Stanford, 7. ed., 2013, Macmillan• Cellular and Molecular Immunology, Abbas, Lichtman, Pillai, 8.ed. 2014, Elsevier Saunders• Script of the lecture


Course 8.5-MBPD-BST: Environmental Biotechnology (EBTE)

Lecturer: Prof. Dr. Thomas Schwartz Place in the curriculum: Elective course in the first or second semester BPD and BST, Level 4 Language: English Teaching form: Lecture and seminar, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: None


Learning objectives: The students • realize the biodiversity of bacteria in their natural habitats and their industrial benefit.• know about the different molecular techniques for taxonomy and phylogeny approaches for prokaryotes.• know about the principles of modern microbial processes during wastewater conditioning (nitrogen

removal, phosphorous removal, activated sludge dynamics, removal of xenobiotica etc.).• know about the technical principles of drinking water conditioning processes (embankment filtration,

flocculation, filtration techniques, disinfection efficiencies etc.).• realize the dynamics of biofilms and their relevance in biotransformation processes, stability of water

compartments, food industries etc.• know about bacteria with relevance for agriculture, environment and biotechnology.• know about antibiotic drug production, bacterial resistance development, and dissemination of antibiotic

resistance genes (horizontal gene transfer in natural systems).

Course content: • introduction in environmental biotechnology and identification of relevant micro-organisms involved in

such processes• performance of different kind of fingerprint techniques for population analysis (i.e. PCR-DGGE, FISH,

ARDRA) in theory and practical course• Relevance of microbes during wastewater and drinking water conditioning• Fundamental biological transformation reactions during conditioning processes (nitrification,

denitrification, enhanced biological phosphorous removal)• Composition, dynamics and signalling via Quorum Sensing molecules in biofilms (EPS, enzymes, eDNA

etc.)• horizontal gene transfer in natural environments (conjugation, transformation, transduction) in concern

of antibiotic resistances and its medical consequences• Application of different fingerprint techniques for population analyses, evaluation and presentation of

the results (report and talk).

Resources and literature: • Script for the lecture and script for the laboratory course• Actual scientific publications for presentations and discussions


Course 8.6-MBPD-BST: Plant Biotechnology (PBTE)

Lecturer: Prof. Dr. Gaby Krczal, Dr. Michael Wallbraun Place in the curriculum: Elective course in the first or second semester BPD and BST, Level 4 Language: English Teaching form: Lecture and seminar, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: General Genetic, Molecular Genetic, General Biochemistry


Learning objectives: The students • know the basics of plant taxonomy and morphology.• understand how to establish transgenic plants by learning about

- tissue culture- construction of plasmids and- plant transformation methods

• know requirements and problems of successful agricultural production, including legislative background.• understand how to engineer resistance against different phytopathological pathogens.• are familiar with the basics about epigenetic gene regulation (transcriptional and post transcriptional

gene silencing).

Course content: • Plant taxonomy•Molecular aspects of plant differentiation (development of embryos and meristemes, flower

differentiation)• Plant tissue culture (micropropagation, production of disease free plant stock, genetic modification of

plant cells)• Plant hormones (mode of action)• Plant transformation (construction of plasmids, Agrobacterium transfer of foreign genes, regeneration of

transgenic plant cells, molecular characterisation of transgenic plants)•New plant breeding techniques• Plant viruses (genome organisation, replication, infection cycle)• Strategies to engineer pathogen resistance (resistances against viruses, bacterias, fungi and insects)• Legal background of producing and marketing transgenic plant material• Transcriptional (TGS) and post-transcriptional gene silencing (PGTS) (induction of TGS and PGTS,

chromatin remodelling, transitivity of gene silencing, RNA-mediated DNA-Methylation, heritability ofepigenetic modifications, viral suppressors of gene silencing)

• Plant regulatory pathways involving non-coding small RNAs (miRNAs, ta-si RNAs, nat-si RNAs)

Resources and literature: • Plant Biotechnology and Genetics: Principles, Techniques, and Applications, Neal Stewart, John Wiley &

Sons Inc;• Plant Biotechnology: The Genetic Manipulation Of Plants, Adrian Slater, Nigel W. Scott, Mark R. Fowler,

Oxford University Press• Lecture notes• (Review) Publications on demand


Course 8.7-MBPD-BST: Transgenic Animals (TGAE)

Lecturer: Prof. Dr. Sigrid Hoffmann Place in the curriculum: Elective course in the first or second semester BPD and BST, Level 4 Language: English Teaching form: Lecture and seminar, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: General Genetic, Molecular Genetic, General Biochemistry


Learning objectives: The students • have a basic understanding in early embryonic development and the female reproductive system.• know the basics of different techniques for transgenesis and pronuclear microinjection.• are able to design transgenic constructs for a specific scientific problem.• know the principals and pitfalls of genotyping and characterization of transgenic animals.• know the breeding procedures for modifying the genetic background for laboratory animals.• are theoretically able to create complex transgenic animals with conditional inducible transgene

expression.• understand the achievements in biomedical research, made with the use of transgenic models.

Course content: • Physiology of reproduction and early embryonic development. The female reproductive system•Methods for gene transfer into the germ line (pronuclear microinjection, retroviral infection, ES cell

manipulation, blastocyst injection, sperm mediated gene transfer, embryotransfer)•Design and cloning of transgenic constructs inducible transgenesis• Characterization of transgene integration and expression in transgenic animals• Knock-out and knock-in techniques (gene targeting constructs, conditional and tissue specific knock-out)• Animal husbandry, breeding strategies and documentation for establishing a transgenic line• Effect of the genetic background on the transgene expression• Rederivation and cryoconservation of the transgenic lines• Strategies to overcome the position effects in transgenesis• Prospects in future (nuclear cloning)•Guidelines for handling of transgenic animals and nomenclatura• Power of transgenic techniques and models in biomedical research - examples

Resources and literature: • Lecture notes• http://www.med.umich.edu/tamc/• http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/T/TransgenicAnimals.html•Manipulating the Mouse Embryo: A Laboratory Manual. Hogan B, Beddington R, Constantini F, Lacy E.

1994. Cold Spring Harbor Press. New York.SCIENCE Book Stacks - QL959 .M2651 1986• Transgenic Animal Technology: A Laboratory Handbook. Pinkert CA, ed. 1994. Academic Press. New York.

SCIENCE Book Stacks - QH 442.6 .T691 1994•Gene Targeting. Sedivy, JM, Joyner, AL. 1992. W.H. Freeman. NY . SC. Book Stacks - QH 442 .S431 1992•Gene Targeting: A Practical Approach, 2nd Edition. Joyner AL, ed. 2000. IRL Press at Oxford University

Press. New York. SCIENCE Book Stacks - QH 442 .G43851 2000.


Course 8.8-MBPD-BST: Human Physiology (HPYE)

Identical to: Course 2.1-MBST

Course 8.9-MBPD-BST: Pharmacodynamics (PHDE)


Course 8.10-MBPD-BST: Pharmacokinetics (PHKE)


Course 8.11-MBPD-BST: Proteomics (PROE)


Course 8.12-MBPD-BST: Modern Analytical Methods (MAME)


Course 8.13-MBPD-BST: Bioinformatics (BINE)


Course 8.14-MBPD-BST: Expression Systems (EXSE)

Identical to: Course 2.1-MBPD

Course 8.15-MBPD-BST: Metabolic Engineering (MBEE)

Identical to: Course 2.2-MBPD

Course 8.16-MBPD-BST: Biosensors (BISE)



Module 9-MBPD-BST: Final Research Project

Code: MTHE and RSEE

Module coordinator: Prof. Dr. Petra Kioschis

Course 9.1-MBPD-BST: Master Thesis (MTHE)

Lecturer: Selected Thesis Advisor Place in the curriculum: Mandatory course in the third semester BPD and BST, Level 4 Language: English Teaching form: Research Project Work load: 900 hours research time and personal learning Credit points: 30 including RSEE Prerequisites acc. to PO: None Recommended prerequisites: Successful completion of all modules of the MSc program

Assessment: 1) evaluation of the thesis2) oral presentation/defense with the censors (RSEE)

Learning objectives: The master’s thesis will demonstrate that the candidate is able to work scientifically on a current research topic in the field within a specified time frame, with an increasing degree of independence applying scientific methods and presenting the results in a scientifically appropriate form. Students in the M.Sc. program must perform a credible original investigation of at least six months under supervision of two major advisers. Students shall prepare a written thesis describing the completed research performed. An oral defense, consisting of a (public) presentation of the thesis and the advisers meeting to discuss the thesis shall be scheduled to examine the student’s research, thesis and underlying fundamental knowledge of the discipline encompassed by the student’s research.

The students • get the opportunity to experience a comprehensive research experience while developing an original

independent project, which allows them to use their knowledge in the pursuit of their own academicinterest.

• gain experimental design competency and thus, are able to design, plan and execute safely an individualresearch project on a current research topic.

• enhance problem-solving skills and will demonstrate an appropriate level of ability to analyze and solvescientific problems.

• can communicate research findings through a written thesis and oral defense.

Course content: • Extensive training in experimental design, the practical use of advanced technologies, data analysis and

interpretation as well as substantial subject-specific knowledge through the project.• Integration and evaluation of information from a variety of sources.•Generation of a comprehensive protocol of own experimental work• Presentation and discussion of results from own experimental studies.

Resources and literature: • Scientific literature and databases related to the project.


Course 9.2-MBPD-BST: Research Seminar (RSEE)

Lecturer: Selected Thesis Advisor Place in the curriculum: Mandatory course in the third semester BPD, Level 4 Language: English Teaching form: Work load: Credit points: Included in MTHE Prerequisites acc. to PO: Research project Recommended prerequisites: Successful completion of all modules of the MSc program

Assessment: seminar talk (30 min) and extended discussion about own experimental work (30 min)

Learning objectives: The students • are exposed to and can discuss ongoing research and published work with their academic advisor and

their research groups.• know and understand concepts, theories, methods and research techniques necessary for accomplishing

her/his educational research project.• demonstrate analytical competencies within the field of research in performing and writing her/his

research project.• have acquired advanced knowledge of academic writing.• can analyse and critically interpret research literature, can collect and analyse empirical data, and create

arguments based on this.•masters the language and terminology of the chosen field of research, and can communicate and discuss

research-based knowledge with educational professionals through academic writing and oralpresentations.

Course content: • Individual support in the development of the research topic.•Written communication skills: appropriate level of written communication skill with respect to grammar,

syntax, spelling and use of vocabulary to effectively present information, including the use of figures,tables and citations.

• Planning structure and solutions for organising a master thesis.• Seminar talk (30 min.) and extended discussion about own experimental work (30 min.).

Resources and literature: • Scientific literature and databases related to the project.


Module 10-MBPD-BST: Optional Research Module

Code: ORME


Lecturer: Course leader of the substituted module Place in the curriculum: Optional course in the first or second semester BPD and BST, Level 4 Language: English Teaching form: Research project, 8 teaching units Work load: 120 hours course time and 180 hours personal learning Credit points: 10 Prerequisites acc. to PO: None Recommended prerequisites: None

Assessment: Independent project work (PA), report (LB) and presentation (PR)

Learning objectives: The module affords students the opportunity to select a preferred independent project work in order to develop and demonstrate their individual research skills and abilities. The optional research module (ORME) is worth 10 credits and can be taken in substitution of one of the compulsory 10 credit practice modules in either the BPD or BST specialization, respectively. The ORME module can be taken in-house, at an external research institution or in industry. The chosen research topic for ORME has to be approved by the course leader of the substituted module. Based on their project choice students will be assigned an academic in-house supervisor, and in case of an external project placement an additional local supervisor. The comprehensive assessment system includes the preparation of a final report, conducting an oral presentation and being assessed by the supervisor(s). The students • attain a level of independent research and creativity.•will develop a working knowledge of the basic principles of responsible research practices.• receive solid training and personal development experience in bioscience and transferable skills and

attitudes.• gain an integrated and critical understanding of research principles and methodologies in biomolecular

sciences and biotechnology.• developed critical skills, both qualitative and quantitative, in the interpretation of data;

put these practical, data analysis and writing skills into practice through undertaking a supervised projectthat reflects their research interest in an area with biotechnology.

• establish contact with research and industrial sectors before starting their Master Thesis.

Course content: • conducting a small independent laboratory project• under supervision, the student participates in a working environment that is relevant to his or her studies• project planning: scheduling activities and estimating their duration• project controlling: recording research results• literature searches using online databases

Resources and literature: • Primary literature: articles, pre-publication prints of articles, conference proceedings• Bioscience and Biotechnology databases


8. Modulbeschreibungen für den Master BME mit Focus BST

Module 1-MBPD-BST: Biostatistics (BSTE)

Identical to: Module 1-MBPD-BST described in Chapter 7


Module 2-MBST: Biomedical Science

Code: HPYE and MOME


Course 2.1-MBST: Human Physiology (HPYE)

Lecturer: Prof. Dr. Norbert Gretz Place in the curriculum: Mandatory course in the first semester BST, Level 4

Elective course in the first or second semester BPD, Level 4 Language: English Teaching form: Lecture, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: None

Assessment: Written examination, 90 min (K90); homework, 28 hours

Learning objectives: The students • are able to explain the interaction of neurons.• can calculate glomerular filtration rate.• can give reasons for hypertension.• are able to explain physiology of ventilation.• can explain the function of different vasoactive hormones.• can explain bone structure and muscle physiology.

Course content: • Basic principles and their coordination, homeostasis and regulation, cellular mechanisms, cellular

membrane transport mechanisms, nerve and synaptic function, autonomic and hormonal control•Nervous system, sensory systems and function, motor functions and their control, integrated functions of

the central nervous system•Gastrointestinal physiology, function of the intestine, pancreas, liver, nutrition• Kidney function, fluid and electolyte balance, glomerular filtration rate, acid-base metabolism•Heart and circulation, electrophysiology of the heart, electrocardiography• Bone structure•Muscle physiology

Resources and literature: •Ganong WF: Review of Medical Physiology•Greger R, Windhorst U: A Comprehensive Human Physiology: from Cellular Mechanisms to Integration•Guyton AC, Hall JE: Pocket Companion to Textbook of Medical Physiology•Marieb EN: Human Anatomy and Physiology•Martini F, Karleskint G: Foundations of Anatomy and Physiology


Course 2.2-MBST: Molecular Medicine (MOME)

Lecturer: Prof. Dr. Petra Kioschis Place in the curriculum: Mandatory course in the first semester BST, Level 4 Language: English Teaching form: Lecture, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: Bachelor level molecular biology, molecular genetics and cell biology


Learning objectives: The students • review the important clinical syndromes associated with complex human diseases.• discuss the relationship between genetic background and predisposition to certain human diseases.• review data concerning the epidemiology of diseases.• understand molecular mechanisms / molecular defects underlying human diseases.• explain key methodologies currently used in molecular diagnostics.• recognize and discuss applications and limitations of molecular diagnostics.• show an awareness of ethical issues associated with the diagnosis of disease.• discuss publications and scientifically present state-of-the-art developments in molecular medicine.• analyze and scientifically discuss future developments of molecular medicine from different

perspectives including emerging techniques and their potential impact on biotechnology research,industry, and clinical practice.

• according to relevant issues of the lecture, students critically analyze and understand the ethical,economic, political, cultural and technical significance of molecular medicine for society.

• according to relevant issues of the lecture, students discuss/communicate in a meaningful fashion theimplications of the various technological and scientific advances from numerous perspectives. Byanalyzing a problem from a different perspective than they are accustomed to, students will developskills that will help them to recognize the many dimensions of problems, and of their solutions as well astheir potential ethical impacts for society.

Course content: • Advances in molecular medicine into the major specialties of medicine.• Overview of disease mechanism• Molecular diagnostic testing• Insights into the pathophysiology and underlying molecular mechanisms of selected common diseases

faced by society today (e.g. cancer, autoimmune diseases, cardiovascular diseases, inflammatorydiseases, neurodegenerative disorders, infectious disease)

• Current treatments and development of new and effective therapies• Modern platform technologies in molecular medicine and their application

Resources and literature: • Genetics and Genomics in Medicine. T. Strachan, J. Goodship, P. Chinnery, 2014• Human Molecular Genetics 4. Taylor & Francis Ltd.; Auflage: 4th ed. 2010• Molecular Diagnostics.G. Patrinos, W. Ansorge, Academic Pr Inc., 2009• Selected publications in the field of molecular medicine and molecular diagnosis• Lecture notes Molecular Medicine• www.ncbi.nlm.nih.gov/Omim/ and others


Module 3-MBST: Cell Science

Code: CPHE and CBAE

Module coordinator: Prof. Dr. Rüdiger Rudolf

Course 3.1-MBST: Cell Physiology (CPHE)

Lecturer: Prof. Dr. Rüdiger Rudolf Place in the curriculum: Mandatory course in the first semester BST, Level 4 Language: English Teaching form: Lecture, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: None

Assessment: Written examination,90 min (K90)

Learning objectives: The students • significantly extend their basic knowledge about eukaryotic cells and their functioning. • learn about fundamental and applied aspects of mammalian cell biology and physiology. • practice scientific discussion.

Course content: • Basic concepts of signal transduction and its dysfunction as major contributor to human disease • Time and spatial scales of signal transduction • Ion channels, biopotentials and channelopathies • Membrane receptors and cellular signaling • Kinases, phosphatases, first and second messengers • Secretory pathway as the major connector between the cell and its environment • Non-membrane bound receptors • Cell cycle and cell death control • Applied signal transduction: sensory systems • Hypothesis conception • Method selection & validation

Resources and literature: • Script • Alberts et al. (2015) Molecular Biology of the Cell. 6th edition. Garland Science. New York, Abingdon.


Course 3.2-MBST: Cell Based Assay Lab (CBAE)

Lecturer: Prof. Dr. Mathias Hafner, Prof. Dr. Petra Kioschis, and Prof. Dr. Rüdiger Rudolf

Place in the curriculum: Mandatory course in the first semester BST, Level 4 Language: English Teaching form: Teaching lab, 6 teaching units Work load: 90 hours course time and 120 hours personal learning Credit points: 7 Prerequisites acc. to PO: None Recommended prerequisites: Undergraduate training in molecular cell biology, basic cell culture

Assessment: Laboratory assessment (LA), seminar and presentation (R)

Learning objectives: The students • are able to verify / reject experimentally a scientific hypothesis / question employing suited methods. • design and set up cell-based assays and understand the limitations of the experimental approach. • learn practical laboratory work in the field of mammalian cellular assays including microscopy in small

groups in a strict time-frame. • interpret experimental data and identify consistent and inconsistent components. • acquire learning skills to continue to study in a manner that may be largely self-directed or autonomous,

as prerequisite to conducting a PhD project in a research setting .

Course content: This module consists of various conceptual lab projects in order to: i) introduce and critically review the concept of modern cell-based screenings; ii) obtain hands-on knowledge of current strategies for cell-based assays, and to introduce target-based and phenotypic assays. The aim is to do so in a conceptual approach: the assays need to be set up independently and according to the methods described in original research articles. • Selected examples:

- Measurement of intracellular free calcium concentration in living cells - Translocation of transcription factor NFkappaB – GFP from cytoplasma to the nucleus - Neuronal differentiation of SH-SY5Y cells - Förster Resonance Energy Transfer (FRET) based protein aggregation assay - Reporter gene assay monitoring proteasomal activity - 3D (tumor) spheroid culture

• Experiment planning and development of working hypotheses • Execution of experiments, including cell culture, live cell microscopy, biomedical image analysis • Data analysis, presentation of scientific talk, discussion of obtained results in the light of literature

Resources and literature: • Selected original publications in the field of mammalian cell based assays. • Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. 6th edition. Freshney.

(2010) Wiley-Blackwell. • Cell Biology Assays. Kreitzer, Jaulin, Espenel (2010) Academic Press. • Molecular Biology of the Cell. 6th edition. Alberts et al. (2015) Garland Science.


Module 4-MBST: Pharmacology

Code: PHKE and PHDE

Module coordinator: Dr. Mario Mezler

Course 4.1-MBST: Pharmakokinetics (PHKE)

Lecturer: Dr. Mario Mezler Place in the curriculum: Mandatory course in the first semester BST, Level 4

Elective course in the first or second semester BDP, Level 4 Language: English Teaching form: Lecture, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: Organic Chemistry

Assessment: Written examination,120 min (K120) – combined with PHDE (1:1)

Learning objectives: The students • know the relevance of pharmacokinetics from early phase drug development to late stage clinical trials• understand the pharmacokinetical characteristics of small molecule drugs and drugs based on biological

molecules.• know about the challenges and opportunities in the pharmaceutical development of biologics including

bioanalytical assays, bioequivalence and exposure-response assessments as well as drug delivery.• have deepened the subject by application of pharmacokinetical concepts into the drug development plan

by means of selected case studies.

Course content: • Pharmacokinetics and Pharmacodynamics in the development of pharmaceutical and biotech drugs• Basic Principles of Pharmacokinetics: absorption, distribution, metabolism, elimination• Pharmacokinetical parameters and models• Pharmacokinetics of peptides and proteins, monoclonal antibodies, antisense nucleotides, viral and non-

viral gene delivery vectors• Bioanalytical methods for pharmacokinetic evaluations of biotech macromolecules• Pharmacokinetics of new drug delivery methods• Examples for the integration of pharmacokinetic and pharmacodynamic concepts in biotech drug

development

Resources and literature: •Goodman, Gilman, The Pharmacological Basis of Therapeutics, McGraw Hill•Meibohm, Pharmacokinetics and Pharmacodynamics of Biotech Drugs, Wiley-VCH•Wu-Pong, Shargel, Yu, Applied Biopharmaceutics and Pharmacokinetcs, McGraw Hill• Rang , Dale, Ritter, Flower, Lamb, Pharmacology, Churchill Livingstone


Course 4.2-MBST: Pharmacodynamics (PHDE)

Lecturer: Dr. Reinhold Müller Place in the curriculum: Mandatory course in the first semester BST, Level 4

Elective course in the first or second semester BDP, Level 4 Language: English Teaching form: lecture, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: Organic Chemistry

Assessment: Written examination,120 min (K120) – combined with PHKE (1:1)

Learning objectives: The students • know the general principles and strategies involved in discovering and designing new drugs and

developing them for the market.• have an overview over the current methods to find drug leads and to use physicochemical properties and

molecular modelling to optimize them.• know the relation of drug structure and solubility and different pharmaceutical and chemical methods for

improvement.• know the types of molecular targets used by drugs.• know the interactions involved when a drug meets a target and the consequences of these interactions.• know the effect of representative drug structures on a wide range of indications.

Course content: •Overview over drug development, sources for drugs, classification of drugs• Lead finding and optimisation• Common methods and routes of drug administration• Basic principles of pharmacodynamics and mechanisms of drug-target interaction•Drugs acting at synaptic and neuroeffector junctional sites•Drugs acting on the central nervous system•Drug therapy of inflammation•Drugs affecting cardiovascular function•Drugs effecting gastrointestinal function• Chemotherapy of microbial diseases• Chemotherapy of neoplastic diseases• Immunomodulators•Hormones and hormone antagonists

Resources and literature: •Goodman, Gilman, The Pharmacological Basis of Therapeutics, McGraw Hill•Wermuth, The principles of Medicinal Chemistry•Mutschler, Arzneimittelwirkungen• Boehm, Klebe Kubinyi, Wirkstoffdesign


Module 5-MBST: Bioinformatics and Proteomics

Code: BINE and PROE

Module coordinator: Prof. Dr. Carsten Hopf

Course 5.1-MBST: Bioinformatics (BINE)

Lecturer: Dr. Friedrich Rippmann, Dr. Eike Staub, Dr. Oliver Karch Place in the curriculum: Mandatory course in the second semester BST, Level 4 Language: English Teaching form: Lecture and tutorial, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: None


Learning objectives: Properties & similarities of biological sequences: • learn about properties of amino acids in relation to similarities, to protein structure and function. • learn about forces relevant to protein structure and function. • learn about the rational principles of sequence alignment, and practical implementations of algorithms

(double dynamic programming). • appreciate the differences between types of homology searches, enabling them to make the right choices

(global versus local alignment; Needleman-Wunsch algorithm vs. Smith Waterman). • learn how to evaluate significance measures for sequence alignments. • understand the origin of insertion sequences and recognize their presence. Protein structure: • be able to visualize and manipulate protein structure files (using PyMOL), structure-sequence coupling

(using Jalview). • learn about the principles of small molecule interactions in the active site of proteins. • understand terminology and methods to describe evolutionary relationships between genes & proteins

(homology, orthology, paralogy). Molecular evolution: • learn to reconstruct evolutionary history from sequence data; understand algorithms like UPGMA,

neighbor joining, maximum parsimony, and their computational limits. • recognize the most frequent systematic artifacts encountered in phylogenetic reconstruction. • understand measures of robustness of phylogenetic trees (non-parametric bootstrapping)

statistics of high-dimensional data. • become familiar with the landscape of methods for biological data mining. • understand when to use which statistical test for analysis of biological data. • become sensitive to problems arising from parallel performance of statistical tests when using high-

dimensional data, become familiar with multiple testing crorrections. Data mining: • recognize the difference between unsupervised and supervised data mining methods, clustering and

classification. • understand basic algorithms to cluster high dimensional genome-scale data. • learn how to assess performance of bioassays (sensitivity, specificity, negative/positive predictive value)

in relevant diagnostic settings. • understand basic methods and workflows to use high-dimensional data for classification of biosamples.

Biological databases.


• obtain an overview on the data-sources supporting the biotechnologists´ daily work.• recognize which database covers which molecular aspect best.• understand how the relevant databases are interlinked.• learn the different technologies and formats used by relevant databases.• learn how to formulate queries to the databases (beyond the user-interface offered).• learn to design a biological database.

Course content: •Databanks at the European Bioinformatics Institute and the National Center for Biotechnology

Information• Algorithms for pairwise & multiple sequence alignment• Fast heuristcs: the different BLAST programs and Position specific iterated BLAST•Homology assessment using Expect-values•Gene structure in pro- and eukaryotes• Inspecting and manipulating protein structures using PyMOL, and structure-sequence coupling (Jalview)• Terminology of molecular evolution (orthology, paralogy, reading a tree)•Heuristics for assignment of orthology• Estimation of evolutionary distances of biological sequences• Reconstruction of phylogenetic trees using distance-based methods (UPGMA, Neighbor-Joining)• Reconstruction of phylogenetic trees using character-based methods (Maximum Parsimony)• Reliability of tree substructures: non-parametric bootstrapping• Speeding-up tree search: branch-and-bound• Analysis of high-dimensional data: basic statistical test & correction for multiple testing•Data normalization methods• Supervised versus unsupervised data mining – introduction to clustering & classification•Hierarchical clustering & K-means clustering• K-nearest neighbor and best-centroid classification• Assessment of classification accuracy: sensitivity, specificity, positive & negative predictive value•Workflows & best practices of data analysis•Overview of relevant biological databases•Database design, implementation, information retrieval

Resources and literature: • Bioinformatics and Functional Genomics, J Pevsner, Wiley• Biostatistical analysis, JH Zar, Prentice Hall• Inferring phylogenies, J Felsenstein, Sinauer Associates•Molecular Evolution, M Nei & S Kumar, Oxford University Press• Bioinformatik: Grundlagen, Algorithmen, Anwendungen, R Merkl, Wiley-VCH


Course 5.2-MBST: Proteomics (PROE)

Lecturer: Prof. Dr. Carsten Hopf Place in the curriculum: Mandatory course in the second semester BST, Level 4 Language: English Teaching form: lecture, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: Instrumental Analytics (IA), Bioanalytics (BAL) or similar


Learning objectives: The students • understand current proteomics technology as well as its utility in the pharmaceutical and diagnostics

industry.• apply their understanding of proteomics technology to understand and solve problems relevant to

research in the pharmaceutical and diagnostics industry.• understand and critically review key primary literature.• understand how to cite scientific literature properly and how to avoid plagiarism.

Course content: • Pharmaceutical industry today: Importance of biomarker - and drug (off-) target discovery• Proteomics Technology:

o Biochemical methods in proteomics: affinity chromatography; (multidimensional) reversed phaseHPLC techniques

o MALDI- and electrospray ionization; LC-MS coupling; nano-HPLCo TOF-, triple quadrupole-, qTOF-, TOF/TOF-, FTICR- and ion trap mass analyzers for proteomicso Protein identification using peptide mass fingerprints, fragment spectra, sequence tags; MASCOT

database searcho Quantitative proteomics methods: SILAC- and iTRAQ-labelling for relative quantification; use of

AQUA peptides for absolute quantification; label-free quantificationo Protein annotation (databases)

• Functional proteomics: analysis of protein-protein interactions and posttranslational modifications;validation of novel drug target candidates by RNA interference

• Chemical proteomics: large-scale analysis of protein-small molecule interactions for drug (off-) targetdiscovery

• Case studies from the recent primary literature

Resources and literature: •Westermeier, Naven, Höpker: Proteomics in Practice – A Guide to Successful Experimental Design, Wiley-

VCH Verlag, 2008• Collection of powerpoint slides• Selection of recent articles from scientific journals


Module 6-MBST: Advanced Bioanalytics

Code: TSAE and ADBE

Module coordinator: Prof. Dr. Carsten Hopf

Course 6.1-MBST: Tissue Analytics (TSAE)

Lecturer: Prof. Dr. Carsten Hopf Place in the curriculum: Mandatory course in the second semester BST, Level 4 Language: English Teaching form: Seminar, 2 teaching units Work load: 30 hours course time and 60 hours personal learning Credit points: 3 Prerequisites acc. to PO: None Recommended prerequisites: Human physiology, molecular medicine, pharmacodynamics

Assessment: Seminar (S) and presentation (R)

Learning objectives: The students • understand the industrial and clinical relevance of tissue analytics, for example the discovery and

monitoring of diagnostic or pharmacodynamic biomarkers; tumor classification in modern ePathology;toxicology and drug safety studies in preclinical drug development.

• can find relevant scientific literature using online search tools and can understand and analyze primaryliterature with respect to recent developments in tissue analytics.

• can present their conclusions from extensive analysis of primary scientific literature in brief, focusedpowerpoint presentations and brief handouts.

• can use their tissue analytics knowledge to discuss it effectively with fellow students.• analyze and evaluate case studies of tissue analytics projects.• understand basic principles of project management and can evaluate and appraise research plans in an

appropriate way.

Course content: • Technology for tissue analytics, e.g. whole body autoradiography, mass spectrometry imaging,

spectroscopic imaging, automated immunohistochemistry•Use cases for tissue analytics in industry and clinics, e.g. discovery and monitoring of diagnostic or

pharmacodynamic biomarkers; tumor classification in modern ePathology; toxicology and drug safetystudies in preclinical drug development

• Tissue analytics as an interdisciplinary science – combining instrumental analytics, pharmacology- andpathophysiology know-how, automation and biostatistics/bioinformatics

• Preparing focused scientific handhouts and powerpoint presentations• Judging the quality of scientific publications and how to cite them properl• Basic project management• Tissue analytics project case studies

Resources and literature: •Handout• Chosen tissue analytics-related articles from the primary literature


Course 6.2-MBST: Advanced Bioanalytics Lab (ABDE)

Lecturer: Prof. Dr. Carsten Hopf and Prof. Dr. Philipp Weller Place in the curriculum: Mandatory course in the second semester BST, Level 4 Language: English Teaching form: Teaching lab, 6 teaching units Work load: 90 hours course time and 120 hours personal learning Credit points: 7 Prerequisites acc. to PO: Biostatistics (BSTE) Recommended prerequisites: Instrumental Analytics (IA) and Bioanalytics (BAL) or similar

Assessment: Laboratory assessment (LA) and presentation (R)

Learning objectives: The students • can use standard bioanalytical sample preparation methods and/or several assays that are currently used

in the industry.• can operate state-of-the-art equipment (e.g. multilabel plate reader with automated sample injection;

infrared spectrometer- or microscope; high-performance thin-layer chromatography; Triple-quadrupole- or MALDI-TOF/TOF- or ESI-QTOF-mass spectrometers; HPLC; gas chromatography - mass spectr. etc.).

• understand quantitative bioanalytical methods (including assays) in industry and clinical application, e.g.biomarker discovery; metabolite quantification in tissue, plasma, plants or food; quantification ofpharmaceuticals or pesticide residues in various complex matrices; identification of metabolites ofpharmaceuticals; cell or product authentication.

• understand limitations of in-vitro experimentation and ethical criteria for studies in animals and humans.• understand what defines a high quality assay or (instrumental) bioanalytical method and can develop

calibration routines (including multivariate calibration models) for assays or (instrumental) bioanalyticalmethods.

• can evaluate different methods and parameter sets with respect to assay/method quality and validation.• can suggest changes to assay/method set-ups to improve assay/method performance.• can write scientifically sound reports and propose standard operating procedures.

Course content: • The course will be embedded in current research projects of the Hopf and Weller research teams

(“forschungsnahe Qualifizierung”).• Small groups of students (2-4) will develop current methods/assays (e.g. product authentication based on

HPTLC; biomarker quantification using LC-MS or GC-MS; cell classification using MALDI-TOF/TOF MS;evaluation of drug-drug interactions using cytochrome P450 assays with luminescent read-out); on one totwo current instruments; during this development, attention will be paid to:

• Key (quality) parameters in assays/methods: signal-to-background; signal-to-noise; z’ value; coefficient ofvariation; inter-assay and intra-assay variability

• bioethical considerations and DFG recommendations for “Safeguarding Good Scientific Practice”• computer programs for biostatistics and (multivariate) data analysis• searching for and working with primary literature• scientific writing of lab reports and standard operating procedures• laboratory safety

Resources and literature: • Chosen tissue analytics-related articles from the primary literature•Method development articles (MALDI, LC-MS/MS, GC-MS etc.)• In some cases: handout


Module 7-MBST: Biosensing and Analytical Technologies

Code: BISE and MAME

Module coordinator: Prof. Dr. Rüdiger Rudolf

Course 7.1-MBST: Biosensors (BISE)

Lecturer: Prof. Dr. Rüdiger Rudolf Place in the curriculum: Mandatory course in the second semester BST, Level 4



Learning objectives: Thestudents • get a broad overview on how biomedical information can be retrieved from samples.• learn about the interface between biological samples and biosensoric molecules/devices.• learn about prerequisites of biosensor set-up, bio-receptors, transduction principles, monitors.• get several examples of the use of biosensors in biomedical research and clinical practice.

Course content: • Biosensors – Definitions• The cell, the body and targets of biosensors•Molecular biosensors and their targets• Chemical biosensors•Genetically encoded biosensors•Detectors for molecular biosensors• Point-of-care and implantation devices•Multiparametric profiling of cellular physiology in vitro and in vivo• Electronic biosensors – general design• Electrochemical biosensors•Optical biosensors• Piezoelectric biosensors• Interferometric biosensors

Resources and literature: • Script• Alberts et al. (2015) Molecular Biology of the Cell. 6th edition. Garland Science. New York, Abingdon.• Renneberg (2009) Bioanalytik für Einsteiger. Spektrum Akademischer Verlag. Heidelberg.• Pawley (2006) Handbook of Biological Confocal Microscopy. 3rd ed. Springer. New York.


Course 7.2-MBST: Modern Analytical Methods (MAME)

Lecturer: Prof. Dr. Philipp Weller Place in the curriculum: Mandatory course in the second semester BST, Level 4


Assessment: Written examination, 90 min (K90) and presentation (R)

Learning objectives: The students • learn to extract information from analytical journals for method development.• learn to apply chromatographic and spectrometric techniques for qualitative and quantitative analytics.• are familiar with modern chromatographic techniques (HPLC, GC, UPLC and derivatives).• are familiar with hyphenation of chromatography to mass spectrometry.• are familiar with typical mass spectrometer types (Quadrupole, TOF, Orbitrap).• understand basic fragmentation pathways in mass spectrometry.• are able to write a proposal for an analytical strategy for a typical bioanalytical problem.

Course content: • Application of chromatographic techniques (HPLC, GC, UPLC)•Mass spectrometry (Quadrupoles, TOF, Orbitrap) and hyphenation concepts• Fragmentation pathways in mass spectrometry• Principles of quantitation in trace and semi-trace levels and method design• Application in bioanalytical scenarios (residue analytics, toxicology, pharmacokinetics)•Method development and lab demo

Resources and literature: • Scientific literature• Lecture slides


Module 8-MBPD-BST: Electives


Module 9-MBPD-BST: Final Research Project (RSEE and MTHE)


Module 10-MBPD-BST: Optional Research Module (ORME)



abkürzungen - hs-mannheim.de · 5.1-mbpd industrial bioprocess development ibde 2 s, r 3/7 (3)...

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