Download - 第三章 信息高分子各论
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. 1. 2. Mendel,(phenotype),,(allele),(gene).
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Morgan,(linkage),(gene map).. 1. :(chromosome),,,(nucleic acid).2.1928, Griffith.1944, Avery.transformation:DNA.
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. DNA1. Chargaff
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2. Blender test
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3. DNA Watson & Crick1953DNA(double helix).P:, dR :G : , C : T :, A :
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4. (central dogma)DNARNA
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DNADNADeoxyribonucleic acid
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Polynucleotide and its componentPurinePyrimidineadenineguaninecytosinethymineuracilriboseadeninedeoxyribosenucleotidenucleotidenucleoside
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. Phosphodiester Bond,1 2 3 4
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. DNADNADNARNA
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. DNA
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. DNA1.
anneal
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2. Melting temperature (Tm)3. pH4. (hybridization)
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. DNADNADNADNANICK
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1. DNA: DNA2. DNA: DNA,,,(linking number, Lk). a. : ,. b. DNA,,,.
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c. :;(ethidium bromide, EB).d. (topoisomerase) I: DNA,,1. II: ATP,DNA,DNA,2.,.
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e. palindromeStemloop structure of DNA
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f. (extinction coeffecient)mgml-1, 1 mgml-1ds DNAA26020,ss DNARNAA26025.:1. ()2.
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RNA. 1. DNA.2. .3. nucleotide,.4. 90%r RNA
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,RNAsplicing
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. :,RNase.
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. RNA
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1. mRNAGenomic DNA,RNA.mRNA. mRNA,,RNA(hnRNA: heterogeneous nuclear RNA)
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2. rRNA
S(Svedberg),,,.
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3. tRNAStem structureLoop structureAmino acidanticodon
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4. snRNA small nuclear RNA,RNAsplicing.mRNAguanine
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5. RNARNA(ribozyme): rRNA,splicing,RNA.,RNA. Ribonuclease P
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.
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. 1.
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2.
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. 1. (primary structure)
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2. (secondary structure)
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3. (tertiary structure)
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4. (quaternary structure)
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. ,,.
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. (domain):.2. ():,-.
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. 1. : . :(gel filtration chromatography)SDS-PAGE
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: (ion-exchange chromatography)(isoelectric focusing) : ,,,. (affinity): His-tag,
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2. HLMGSHLVDALELVMGDRGFEYTPKAWLVV8T1: HLMGSHLVDALELVMGDRT2: GFEYTPKT3: AWLVV1: HLMGSHLVDALET2: LVMGDRGFET3: YTPKAWLVHLMGSHLVDALELVMGDRGFEYTPKAWLVEdman degradation
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3. SDS-PAGEMALDI-TOF MS (Matrix Assisted Laser Desorption Ionization, Time Of Flight MASS )
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4. X5. .6. (proteome) .
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Questions
,.,.