No.G013-1402

RNA

lncRNA-seq RNA

RNAlong noncoding RNAlncRNA

RNA200nt

RNAII

lncRNA

DNARNA

m i R N A

lncRNA

rRNAlncRNAmRNA

lncRNAmRNA

lncRNAmRNA

10G

polyAmRNAmRNA

RNApolyARNA

5G

RNA-seq

mRNA

DGE

RNA-seqDGE

10M reads

Small RNARNA,

20-30miRNApiRNA

siRNAsnoRNARNASmall RNA

RNA

RNA(microRNA, miRNA)small RNA

, , mRNA

piRNA(PIWI-interacting RNA)

RNAPIWI

2632DicerpiRNA

piRNA

piRNAmicroRNAs

piRNA

10M reads

1.

- 1 -

Small RNA-seq RNA

(DGE)

polyA-seq mRNA

No.G013-1402

l ncRNA200

RNAlncRNA

lncRNA

0.75h3h4.5h6h10h28h48h

120h10002

10

polyARNA

200-300M reads

lncRNAmRNA

lncRNA

lncRNA(Andrea Pauli et al., Genome Research. 2012)

8

[14d14d

]

GAII

8-9M reads/

33%

234

1. microRNA

RNAGuojie Zhang et al., Genome Research. 2010

1.

2. lncRNA

835lncRNAmRNA

GO10GO

268

4-7lncRNA

3lncRNA

lncRNA

4

3. lncRNAmRNAlncRNA

4. lncRNAGOlncRNA lncRNA

2.

1 - K+transmembrane transport,K+channel activity,Oxidoreductase activity,Voltage-gated K+channel complex

2 - Neuropeptide signaling,Ligand-dependent nuclear receptor activity,Cell junction

3 - Growth factor activity,Structural molecule activity,Cell adhesion,Tricarboxylic acid cycle

4 - Blood vessel development,Heart contraction,Growth factor activity,Multicellular organism development

5 - Proteasome complex,Muscle organ development,Ribonucleoprotein complex,Translation

6 - Retina Development,Embryonic pattern specification,Pancreas development

7 - Embryonic heart tube morphogenesis,RNA binding,Cytokinesis,Mesoderm development

8 - Cell cycle,Mitosis,DNA repair,Transcription initiation from RNAPLL promoter

9 - Response to DNA damage stimulus,Histone binding,Cell division,Protein-S/T kinase activity

10 - Histone binding

10GO

5. AmiRNABC 1KD

CA

B

D

- 2 -

2. KEGG

6

4BCG/Ad853

3

14PBMC

PPD-B 24h

GAIISE50

28M reads

IFNGIL22

1. PPD

RNA

Sabin Bhuju et al., PLOS Pathogens. 2012

Akshay Kakumanu et al., Plant Physiology. 2012

8. A B IFNGIL22

2. 7

6. 7

3

7. reads A BC

A

B

C

A B

No.G013-1402

- 3 -

8

GAII32M reads

10. MapManAB

9. GO GO

1. GO

:

miRNA

RNA

42162

Small RNAmiRNALi Kang et al., BMC Genomics. 2013

1. miRNA

2. MapMan

A B

12. 93miRNAGO

2. miRNA GO

No.G013-1402

small RNAmiRNA

14M reads

202miRNA93

miRNA

- 4 -

11. (A)42162miRNA(B) 15miRNA

A B

piRNA

CREBDNA

piRNA

AplysiaCNSRNA

piRNA

piRNA28

piRNA

5ug RNA

RNA

piRNA

PIWI/piRNA

CREB2CpG

RNA

piRNARajasethupathy, P. et al., Cell. 2012

lncRNA

DNA

DNAlncRNA

NAT natural antisense transcript

NAT

Huan Wang et al., Genome Research. 2014

13. RNA piRNA

15. CREB2CpG ESTsCREB2piRNA piRNAESTs

14. 372piRNA piRNA piRNAU(T)

No.G013-1402

1. NAT

16. lncNATlncNAT

CB

ED

GF

A

Col-0

Illumina HiSeq 2500

SR10038-48M reads/

37,238 NAT

ATH NAT

1,392NAT

NAT

Arabidopsis thaliana

- 5 -

Andrea Pauli, Eivind Valen, Michael F. Lin, et al., Systematic identification of long noncoding RNAs expressed during zebrafish embryogenesis. Genome Research. 2012; 22:577-591.

Guojie Zhang, Guangwu Guo, Xueda Hu, et al., Deep RNA sequencing at single base- pair resolution reveals high complexity of the rice transcriptome. Genome Research. 2010; 20:646-654.

Sabin Bhuju, Elihu Aranday- Cortes, Bernardo Villarreal- Ramos, et al., Global Gene Transcriptome Analysis in Vaccinated Cattle Revealed a Dominant Role of IL- 22 for Protection against Bovine Tuberculosis. PLOS Pathogens. 2012; 8(12):e1003077.

Akshay Kakumanu, Madana M.R. Ambavaram, Curtis Klumas, et al., Effects of Drought on Gene Expression in Maize Reproductive and Leaf Meristem Tissue Revealed by RNA- Seq. Plant Physiology. 2012; 160: 846-867.

Li Kang, Xinxing Cui, Yujie Zhang, et al., Identification of miRNAs associated with sexual maturity in chicken ovary by Illumina small RNA deep sequencing. BMC Genomics. 2013; 14:352.

Priyamvada Rajasethupathy, Thomas Tuschl, Eric R. Kandel, et al., A Role for Neuronal piRNAs in the Epigenetic Control of Memory- Related Synaptic Plasticity. Cell. 2012; 149: 693-707.

Huan Wang, Pil Joong Chung, Jun Liu, et al., Genome- wide identification of long noncoding natural antisense transcripts and their responses to light in Arabidopsis. Genome Research. 2014. Published online January 8, 2014 in advance of the print journal. doi:10.1101/gr.165555.113.

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2.

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5.

6.

7.

GUANGZHOU RIBOBIO CO., LTD

/add182C313-14510663 /tel02032290075 /fax02032290137400 686 0075 QQ400 686 0075 /http: www.ribobio.com /Email: marketing@ribobio.com : RiboBio

2.NAT

NAT

17. (A) 1h 6h(log2 ) (B) lncRNAs lncNATs(C )(D) qRT-PCRlncNATs(E) (F )qRT-PCR (SE) (n = 3); (C) (H) (R) (D) (1) 1h (6) 6h

3.NAT

18. H3K9acNAT(A,B) ATH NAT qRT-PCRNAT(C) AT2G18280 and AT2G18290 H3K9ac

A B

C D

E F

No.G013-1402

B

A

C

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