School of Life SciencesAnnual Research Symposium

Crieff Hydro Hotel 16th-18th March 2017

Scientific Posters1 ‘An Affinity-Directed PROtein Missile (AdPROM) System for Targeted

Proteolysis of Endogenous Proteins’ Luke J Fulcher, Luke D Hutchinson, Thomas Macartney, Polina Bozatzi, Annika Hornberger, Craig Turnbull, Alejandro Rojas Fernandez, Gopal P Sapkota

2 ‘Root Hairs are a Determinant of the Rhizosphere microbiota’ Senga Robertson-Albertyn, Katharin Balbirnie and Davide Bulgarelli

3 ‘CMTR1 stimulated translation is influenced by DHX15 to regulate cell proliferation’ Francisco Inesta Vaquera, Alison Galloway and Victoria H Cowling

4 ‘ Protein complexes in T. brucei and changes in the proteome across the cell cycle’ Thomas W. M. Crozier; Michele Tinti; Angus I. Lamond; Michael A. J. Ferguson

5 ‘Probing the role of PINK1/Parkin’ Kristin Balk, Alban Ordureau, Thomas Macartney, Wade Harper and Miratul Muqit

6 ‘Making Biological Data Work for You’ Chris Cole, Marek Gierlinski and Geoff Barton

7 ‘mRNA cap methyltransferases in T cell activation’ A Galloway, A Kaskar, V H Cowling

8 ‘Direct Detection of O-GlcNAc Glycosylation Stoichiometry and Dynamics Using an Affinity Retardation Gel’ Chuan Fu, Daan van Aalten

9 ‘DNA replication and Epigenome maintenance’ Constance Alabert10 ‘Understanding pea aphid virulence using Next-generation sequencing

and genetics’ S. Altmann, S. Kanvil, P. Thorpe, C. Turnbull, J. Bos11 ‘Strategies to determine the effects of subunit loss from the

mammalian SWI/SNF complex’ Seraina Blümli, Nicola Wiechens and Tom Owen-Hughes

12 ‘Mechanisms of the initial kinetochore-microtubule interaction in human cells’ Alexander JR Booth, John K Eykelenboom, Helfrid Hochegger, Tomoyuki U Tanaka

13 ‘Molecular control of Rnf12 function in pluripotency and disease’ Francisco Bustos, Lennart Brandenburg, Anna Segarra-Fas, Rachel Toth, Helen Walden and Greg Findlay

14 ‘Understanding PP1 and PP2A-B56 specificity at kinetochores’ Marilia H. Cordeiro*, Richard J. Smith* and Adrian T. Saurin (*equal contribution)

15 ‘Identifying highly divergent glycosyltransferases in the African trypanosome’ Samuel M Duncan and Michael AJ Ferguson

16 ‘Biomarkers in Parkinson’s disease’ Ying Fan, Andy Howden, Francesca Tonelli, Genta Ito, Dario Alessi, Esther Sammler

17 ‘Distinct Brd2-Brd4 functionalities coordinate pluripotent exit with Smad2-dependent lineage specification’ Rosalia Fernandez-Alonso, Lindsay Davidson, Jens Hukelmann, Charles A. C. Williams, Michael Zengerle, Alan R. Prescott, Angus Lamond, Alessio Ciulli, Gopal P. Sapkota and Greg M. Findlay

18 ‘Is the centromere localisation of Aurora B kinase essential for chromosome bi-orientation?’ Luis J García Rodríguez and Tomoyuki Tanaka

19 ‘Using cysteine-GlcNAc as a non-hydrolysable O-GlcNAc mimic’ Andrii Gorelik, Vladimir Borodkin, Andrew Ferenbach and Daan van Aalten

20 ‘Accelerating barley breeding with Genomic Selection’ Ruth Hamilton, Claire Halpin, Robbie Waugh, Helena Oakey, Bill Thomas, Hazel Bull

21 ‘Investigation of molecular mechanism of biofilm formation by Listeria monocytogenes’ Chih-Yu Hsu, Lynne Cairns, Katharina Schleicher, Laura Hobley, Nicola Stanley-Wall

22 ‘The regulation of TGF-beta signalling by salt-inducible kinases (SIKs)’ Luke D. Hutchinson, Dr. Nicola Darling, Prof. Philip Cohen and Dr. Gopal P. Sapkota

23 ‘Omics-Enabled Detection and Studies of Phytophthora capsici in the Field’ Rory McLeod, Christiaan Cornelisse, Leighton Pritchard, Petra Bleeker, Edgar Huitema

24 ‘Dangerous sweets - Elucidation of the group A carbohydrate biosynthesis pathway’ Azul Zorzoli, Benjamin Meyer, Melanie Schmittner, Helge Dorfmueller

25 ‘Role of ubiquitylation in the termination of DNA replication in budding yeast’ Progya Mukherjee, Marija Maric, Michael Tatum, Ron Hay and Karim Labib

26 ‘To Determine the Protein Dynamics Associated with RNF4-Mediated Ubiquitin Transfer’ Paul Murphy

27 ‘Studying the role of Bod1 in the development of iPS-derived neuronal tissue’ Porter IM, Porter M, Davidson L and Swedlow JR

28 ‘Role of O-GlcNAc transferase TPR repeats in glycosylation substrate recognition’ Olawale Raimi, Karim Rafie, Vladimir Borodkin, Andrew T. Ferenbach, Daan M.F. van Aalten

29 ‘The regulation and function of the transcription factor NFIL3 in CD8+ T cells’ Christina Rollings, Sarah Ross, Hugh Brady and Doreen Cantrell

30 ‘The functional characterisation of APETELA2-Like Transcription Factors in Barley’ Jennifer Shoesmith, Sarah McKim and Kelly Houston

31 ‘Regulation of amino acid transporter SLC7A5 couples methionine metabolism to the epigenetic control of T cell differentiation’ Linda V Sinclair, Jens L Hukelmann, Sarah Thomson, Andrew N Macintyre, Peter M taylor, Jeffrey C Rathmell, Jason W Locasale, Angus I Lamond and Doreen A Cantrell

32 ‘Developing ways to control localised phosphatase activity’ Richard J. Smith, Giulia Vallardi and Adrian T. Saurin

33 ‘Investigating the function and regulation of FAM83H, a gene that is mutated in Amelogenesis Imperfecta’ Theresa Tachie-Menson, Luke Fulcher, Gopal Sapkota

34 ‘Understanding the basis for PP2A-B56 isoform-specificity’ Giulia Vallardi and Adrian T. Saurin

35 ‘Dimerisation dynamics of cytokine receptors’ Stephan Wilmes, Ignacio Moraga, Zhi Li, Maximillian Hafer, Christopher Garcia, Sandra Pellegrini and Jacob Piehler

36 ‘Investigating the protein composition of PBRM1-deficient SWI/SNF complexes in renal cell carcinoma’ Meng-Ying Wu, Seraina Blümli, Nicola Wiechens and Tom Owen-Hughes

Support Services 37 ‘The FACS of Life (sciences)’ Rosie Clarke, Arlene Whigham, Mercede

Lee and Doreen Cantrell38 ‘The FingerPrints Proteomics Facility - From Identification through

Characterisation to Large Scale Quantitation’ Abdelmadjid Atrih, Kenneth A Beattie, Wenzhang Chen, Samantha Kosto, Amy L Tavendale, Karolina Wrobel and Douglas J Lamont

39 ‘Public Engagement in SLS: a world of opportunities!’ Amy Cameron and Erin Hardee

40 ‘The National Phenotypic Screening Centre: Open-access Platforms for High throughput Quantitative Biology and Drug Discovery’ Paul Andrews, Zoe Gage, Emma Gutcher, Alistair Langlands, John Raynor, Neil Carragher, Daniel Ebner, Den Barrault, Andrew Hopkins

Image “A primary adult lung fibroblast from the mito-QC reporter mouse” by Prescott, McWilliams & Ganley

Programme - Thursday 16th March0840-0950 Hotel Check-in (Ballroom)

Coffee and Registration (The Hub)

Session 1 – Chaired by Kate Storey

0950-1000 Introduction and welcome by Julian Blow

1000-1030 Ian Gilbert (BCDD)“Anti-infective drug discovery in the DDU”

1030-1100 Jason Swedlow (GRE)“Adventures in Cell Divison and Data Publication”

1100-1130 Sebastian Eves-van den Akker (PS)“Tumour formation in plants by parasitic nematodes”

1130-1145 SLS Resources: Sam Swift“Technology and resources in the Dundee Imaging Facility”

1145-1200 SLS Resources: Paul Andrews“Interrogating Complex Biology at Scale in the National Phenotypic Screening Centre: From Microbes to Man”

1205-1330 Lunch (Meikle Restaurant)

Session 2 – Chaired by John Rouse

1330-1400 Satpal Virdee“Shaking down E3 ligases with activity-based probes”

1400-1430 David McEwan (CSI)“Autophagy machinery in health and disease”

1430-1500 Guest speaker: Adrian Saurin (Medicine)“How protein phosphorylation signals are regulated and why the textbooks are misleading”

1500-1530 Tea and Coffee

1530-1600 SLS showcaseLuke Fulcher (PPU/GS): AdPROM-mediated endogenous protein degradation (Poster 1)Senga Robertson-Albertyn (PS/DB): Root Hairs and Rhizosphere microbiota (Poster 2)Francisco Inesta-Vaquera (GRE/VC): Fitting your CAP: CMTR1 regulation (Poster 3)Michele Tinti (BCDD/MAJF): T. brucei proteome changes over cell cycle (Poster 4)

Programme - Friday 17th March0730-0930 Breakfast (Meikle Restaurant)

Session 3 – Chaired by Ron Hay

0900-0930 Doreen Cantrell (CSI)“Shaping the T cell proteomic landscape by antigen receptor and cytokines”

0930-1000 Geoffrey Gadd (CSI/Geomicrobiology Group)“Geomycology: metals, minerals and fungi”

1000-1030 SLS showcaseKristin Balk (PPU/MM): Probing the role of PINK1/Parkin (Poster 5)Chris Cole (CB/GB): Making biological data work for you (Poster 6)Alison Galloway (GRE/VC): RNA cap methyltransferases in T cell activation (Poster 7)Chuan Fu (GRE/DVA): Detecting O-GlcNAc Stoichiometry by Gel (Poster 8)

1030-1100 Tea and Coffee

1100-1130 Gopal Sapkota (MRC-PPU)“Of Frogs, Mice and Men: The story of PAWS1 so far”

1130-1200 Claire Halpin (PS)“Gee-whiz GWAS”

Programme - Saturday 18th March0730-0900 Breakfast (Meikle Restaurant)

Session 4 – Chaired by Anton Gartner

0900-0930 Karim Labib (MRC-PPU)“The End of Chromosome Replication”

0930-1000 Pauline Schaap (CDB)“Why Dictyostelium stalk formation always occurs at the organizer of cell movement.”

1000-1030 Guest speaker: Michael MacDonald (Physics/Medicine)“Shaping light for imaging and manipulation in the life sciences”

1030-1100 Tea and Coffee

1100-1130 Robbie Waugh (PS)“Barley: Adaptation to different environments”

1130-1200 Tom Owen-Hughes (GRE)“Alterations to chromatin remodelling complexes in cancer”

1200 Closing remarks from Claire Halpin

Session 2 – cont.

1600-1630 Nicola Stanley-Wall (MMB)“Constructing and protecting bacterial communities”

1630-1700 Inke Nathke (CDB)“Mechanisms regulating epithelial tissue structure and function in the intestine”

1700-1710 Athena SWAN (Inke Nathke)

1710-1720 Summer School (Anton Gartner)

1720-1730 SLS Awards Presentation by Julian Blow

1900-2100 Dinner (Meikle Restaurant)

2000-2300 Ceilidh (Ballroom)

Session 3 – cont.

1200-1300 KEYNOTE LECTURER: Prof. Jay D. Keasling (Departments of Chemical Engineering and Bioengineering, University of California, Berkeley. Synthetic Biology Department, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, and Joint BioEnergy Institute, Emeryville)

“Engineering Microbial Metabolism for Production of Chemicals and Fuels”

1300-1430 Buffet Lunch (outside Drawing Room)

1300-1600 Poster Session (Drawing Room)

1900-2100 Dinner (Meikle Restaurant)

2000-2300 Disco (Ballroom)