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Zika virus1947Flaviviridae)FlavivirusRNA1947196819841966 20072010Haddow AD, Schuh AJ, Yasuda CY, Kasper MR, Heang V, Huy R, et al. (2012) Genetic Characterization of Zika Virus Strains: Geographic Expansion of the Asian Lineage. PLoS Negl Trop Dis 6(2): e1477. doi:10.1371/journal.pntd.0001477

200747Federated States of MicronesiaYap>3732013102014211

20072013201552015

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/2015.05WHO

http://stacks.cdc.gov/view/cdc/37467

http://www.cdc.gov/zika/geo/

05102~7()3~124IgM80

mosquito byLuis Prado

Trans R Soc Trop Med Hyg. 1983Ae. hensiliiwas the predominant mosquito species present on Yap during the ZIKV disease outbreak in 2007, but investigators were unable to detect ZIKV in any mosquitoes on the island during the outbreak10

80/non-purulent / hyperemic

Yap

Duffy MR, et al. N Engl J Med 2009;360:2536-43.

4IgMIgM1440Plaque reduction neutralization test (PRNT) : Fixed tissues: Immunohistochemical (IHC) staining for viral antigens or RT-PCR

Reverse transcriptase-polymerase chain reaction (RT-PCR) for viral RNA in serum collected 7 days after illness onset

Serology for IgM and neutralizing antibodies in serum collected 4 days after illness onset

Plaque reduction neutralization test (PRNT) for 4-fold rise in virus-specific neutralizing antibodies in paired sera

Only available at CDC

Immunohistochemical(IHC) staining for viral antigens or RT-PCR on fixed tissues

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IgMneutralizing antibody

Zika virus serology (IgM) can be positive due to antibodies against related flaviviruses(e.g., dengue and yellow fever viruses)

Neutralizing antibody testing may discriminate between cross-reacting antibodies in primary flavivirusinfections

Difficult to distinguish infecting virus in people previously infected with or vaccinated against a related flavivirus

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acetaminophenaspirin nonsteroidal anti-inflammatory drugs (NSAIDs)NSAIDs

27Guillain-Barre Syndrome (GBS)

4idiopathic thrombocytopenic purpura (ITP)21http://www.hygiene-publique.gov.pf/IMG/pdf/bulletin_zika-dengue_.pdf8,50329,00067

2641Guillain-Barre Syndrome

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(AP Photo/Felipe Dana)

201520

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21WHO1201520162014PHEIC[Public Health Emergency of International Concern]PHEIC/WHO EISWHO

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1. arthralgiaarthritisnon-purulent /hyperemic conjunctivitis2. *

*33rd percentile1.

2. A. B. A1+B1 A1+B2 A2B

IgM IgG IgM IgG 4 1.

2. C. B. C1 C2 C4B1+C3 B2+C3

DEETDEET

DEET

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DEET

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Zika virus in semen44-year-old man in Tahiti developed asthenia, low grade fever and arthralgia8 weeks later: fever, headache, arthralgia2 weeks later: hematospermiaBlood: rRT-PCR for ZIKV negativeSemen: rRT-PCR for ZIKV positive3 days laterBlood: rRT-PCR for ZIKV negativeUrine: rRT-PCR for ZIKV positiveSemen: rRT-PCR for ZIKV positiveMusso D, Roche C, Robin E, Nhan T, Teissier A, Cao-Lormeau VM. Potential sexual transmission of Zika virus. Emerg Infect Dis. 2015 Feb [date cited].http://dx.doi.org/10.3201/eid2102.141363

Zika virus in BloodStudy conducted using blood donated during outbreak of Zika virus in French PolynesiaBlood donated during November 2013 to February 2014 were testedAll blood donors were asymptomatic for Zika virus infection42 (2.8%) of 1,505 donated blood were found positive for ZIKAV by PCRMusso D, Nhan T, Robin E, Roche C, Bierlaire D, Zisou K, Shan Yan A, Cao-Lormeau VM, Broult J. Potential for Zika virus transmission through blood transfusion demonstrated during an outbreak in French Polynesia, November 2013 to February 2014 . Euro Surveill. 2014;19(14):pii=20761. Article DOI: http://dx.doi.org/10.2807/1560-7917.ES2014.19.14.20761

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22144634, 5621arthralgiaarthritisnon-purulent /hyperemic conjunctivitis22123123IgMIgG44546151

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TORCH: toxoplasma, OTHERS, rubella, CMV, HSVOtherssyphilis, varicella-zoster, parvovirus B19

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syphilistoxoplasmosisrubellacytomegalovirus [CMV]lymphocytic choriomeningitis virus [LCMV]herpes simplexASTALTbilirubin

(evoked otoacoustic emissions testing)(auditory brainstem response testing)

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